2011-09 Instructions for Use for INFINITE M1000 PRO No. 30064852 Rev. No. 1.0 7
4.4Luminescence System........................................................43
4.4.1Luminescence Optics ............................................................44
4.4.2Luminescence Detection .......................................................46
4.5AlphaScreen/AlphaLISA System........................................47
4.5.1AlphaScreen/AlphaLISA Optics.............................................47
4.5.2AlphaScreen/AlphaLISA Detection........................................49
4.5.3AlphaScreen/AlphaLISA Temperature Correction .................49
5.Operating the INFINITE M1000 PRO......................................... 51
5.1Introduction .........................................................................51
5.2General Operating Features ...............................................52
5.2.1Instrument Start Up................................................................52
5.2.2Finish a Measurement Session..............................................53
5.2.3General Options.....................................................................53
5.3Optimize Fluorescence Measurements .............................54
5.3.1FI Scanning (Spectral Intensity Calibration)...........................54
5.3.2FP Measurements .................................................................55
5.3.3Instrument Parameters..........................................................57
5.3.4FI Ratio Mode........................................................................62
5.3.5Optimal Read (FI Bottom Measurements Only).....................62
5.3.6Measurement Accessories ....................................................64
5.4Optimize Absorbance Measurements................................64
5.4.1Measurement Parameters.....................................................64
5.4.2Absorbance Ratio Mode........................................................65
5.4.3Measurement Accessories ....................................................65
5.5Optimize Luminescence Measurements ...........................65
5.5.1Integration Time.....................................................................65
5.5.2Light Level Attenuation..........................................................66
5.6Optimize AlphaScreen/AlphaLISA .....................................67
5.6.1Excitation Time......................................................................67
5.6.2Integration Time.....................................................................67
5.7Injectors ...............................................................................68
5.7.1Measurement with Injectors...................................................69
5.7.2Storage Bottles and Bottle Holders........................................70
5.7.3Injector Carrier.......................................................................71
5.7.4Priming and Washing of the Injector(s)..................................72
5.7.5Injector Modes and Settings (i-control)..................................77
5.7.6Injector Cleaning and Maintenance .......................................81
5.7.7Injector Reagent Compatibility...............................................83
5.8Built-in Stacker ....................................................................84
5.9Barcode Scanner .................................................................85
6.Instrument Specifications ......................................................... 87
6.1Introduction .........................................................................87
6.2Technical Specifications.....................................................88
6.3Fluorescence Intensity and Time Resolved Fluorescence
(TRF) .....................................................................................89
6.3.1Definition of the Detection Limit:............................................89
6.3.2Fluorescein (Fluorescence Intensity) Top..............................89
