Serva BlueVertical PRIME Manuel utilisateur

eLine

Instruments for Electrophoresis

INSTRUCTION MANUAL

BlueVertical™ PRiME™

Vertical Electrophoresis Chamber

SERVA Electrophoresis GmbH �Carl-Benz-Str. 7 �D-69115 Heidelberg

Phone +49-6221-138400, Fax +49-6221-1384010

e-mail: info@serva.de �http://www.serva.de

DOMINIQUE DUTSCHER SAS

WARNING

These units are capable of delivering potentially lethal voltage when

connected to a power supply and are to be operated only by qualified

technically trained personal.

Please read the entire operator’s manual thoroughly before operating this

unit.

The BlueLine vertical electrophoresis systems are designed to give long

service and reproducible results in your laboratory. A few moments spent

reading these instructions will ensure that your expectations are reflected in

the successful use of the apparatus.

First check that the apparatus has been received complete and undamaged following

shipment. Any faults or losses must be notified to responsible SERVA Electrophoresis

GmbH distributor immediately. SERVA Electrophoresis GmbH Heidelberg cannot

accept responsibility for goods returned without prior notification.

Refer to the packing list and check that all components and accessories are present.

Warranty is 12 months from the date of delivery.

Please retain all packaging materials until the warranty period has expired.

Ver. 02/13

DOMINIQUE DUTSCHER SAS

Contents

1. Packing List 4

2. Specifications 4

3. Operational conditions 5

4. Using the vertical gel electrophoresis unit 5

4.1. Safety precautions 5

4.2. General care and maintenance 6

4.3. Gel and buffer volumes/running conditions 6

4.4. Performing electrophoresis 7

4.4.1. Insertion of pre-cast gel cassettes into the inner core running unit 7

4.4.2. Sample loading 8

4.4.3. Start of electrophoresis 8

4.4.4. End of run 8

5. Recommended reagents for vertical electrophoresis 10

DOMINIQUE DUTSCHER SAS

1. PACKING LIST

BlueVertical™ PRiME™

Cat. no.: BV 104

No. of

Items Description Cat. no.

1 Main Unit BV 104

1 Dummy Plate BV 104-7

2. Specifications

• Rugged acrylic construction

• All acrylic joints chemically bonded

• Doubly insulated cables, rated safe up to 1000 volt

• Gold plated electrical connectors, corrosion-free and rated safe up to 1000 volt

• Safety lid with integrated power connectors

• Pure platinum electrodes (0.2 mm diameter)

• Size: 16 cm x 15.6 cm x 9.5 cm (w x h x d)

• Weight: 1.2 kg

DOMINIQUE DUTSCHER SAS

Table 1: Specifications

Volume inner buffer chamber 200 ml

Voltage (max) 500 V

Current (max) 250 mA

Operating temperature 4 °C – 65 °C

Electrodes Platinum wire (0.2 mm, 99.99 % pure)

Dimensions 16 x 15.6 x 9.5 cm (WxHxD)

Weight 1.2 kg

3. Operational conditions

This apparatus is intended for indoor use only. Maximum relative humidity up to

80 % (for temperatures up to 31 °C) decreasing linearly to 50 % relative humidity

(for temperatures up to 40 °C), at maximum altitude of 2000 m (MSL).

4. Using the vertical gel electrophoresis unit

4.1. Safety precautions

• Read the instructions carefully before using the apparatus.

• Always disconnect the electrophoresis unit from the power supply before removing

the safety cover.

• Do not attach the safety lid to the internal gel running unit while it is out of the

bottom buffer chamber.

• Do not exceed the maximum operating voltage or current (see Table 1).

• Do not operate the electrophoresis units in metal trays.

• Polymerised gels may contain some unpolymerised acrylamide monomer, which is

a volatile, cumulative neurotoxin and suspected carcinogen. Wear effective

protective clothing and gloves. Follow recommended handling and disposal

procedures.

• Do not fill the unit with running buffer above the maximum fill lines.

• Do not move the unit when it is running.

• Caution: During electrophoresis very low quantities of various gases are produced

at the electrodes. The type of gas produced depends on the composition of the

buffer employed. To disperse these gases make sure that the apparatus is run in

a well ventilated area.

DOMINIQUE DUTSCHER SAS

4.2. General care and maintenance

• Before use clean and dry the apparatus with distilled water only.

Important: Acrylic plastic is not resistant to aromatic or halogenated

hydrocarbons, ketones, esters, alcohols (over 30 %) and acids (over 25 %).

• Before use, and then on a monthly basis, check the unit for any leaks at the

bonded joints. Place the unit on a sheet of dry tissue and then fill with distilled

water only to the maximum fill line. Any leakage will cause dampening of the

tissue paper. If any leakage is seen do not attempt to repair or use the

apparatus, but notify SERVA Electrophoresis GmbH Heidelberg resp. the

SERVA Electrophoresis GmbH distributor immediately.

• The platinum electrodes are partially shrouded for protection. However, when

cleaning the main tank do not use cleaning brushes in the electrode area. Usually

a thorough rinse with distilled water is all that is required.

• Ensure that the connectors are clean and dry before usage or storage.

4.3. Buffer volume/running conditions

The buffer volume can be seen in Table1. The running conditions (Table 2) vary

according to the number of gels and their composition. The current required will increase

in proportion to the number of gels providing that the voltage is not limiting, e. g. two gels

require twice the current of one but the same voltage.

By increasing the gel concentration the electrical resistance is increased and the rate of

migration decreases. Higher voltages can be applied but be careful not to overheat the

gel.

Table 2: Running conditions using BlueVerticalTM in different electrophoresis applications

Type of electrophoresis Type of SERVAGel

Precast Gel Running conditions

SDS PAGE SERVAGelTM HSE • Voltconst.: 400 Volt

• Time: 20 minutes

SDS PAGE SERVAGelTM TG PRiMETM • Voltconst.: 300 Volt

• Time: 35 minutes

SDS PAGE SERVAGelTM TG Neutral • Voltconst.: 150 Volt

• Time: 70 minutes

Native PAGE SERVAGelTM N • Voltconst.: 130 Volt

• Time: 60 – 120 minutes

Isoelectric Focusing SERVAGelTM IEF

• Voltconst.: 100 Volt for 60 minutes

• Voltconst.: 200 Volt for 60 minutes

• Voltconst.: 500 Volt for 30 minutes

•

For detailed information about the running conditions of SERVAGel™precast vertical mini

gels, please see the manuals supplied with the gels.

DOMINIQUE DUTSCHER SAS

4.4. Performing electrophoresis

4.4.1. Insertion of precast gel cassettes into the inner core running unit

•Clean the silicone gasket, located in the inner core unit. If the gasket becomes

unseated from its groove simply press it back into place.

•Insert the precast gel cassette into the inner core running unit.

•When running only one gel, a dummy plate is required on the opposite side to

retain the required buffer level.

•Place the gel running unit into the buffer tank using the locating pegs as guides.

•Add the appropriate volumes of running buffer to the inner and outer buffer

chambers (see Table 1).

•Important: Do not overfill (please see marking “max fill”).

1 2 3

4 5 6

DOMINIQUE DUTSCHER SAS

4.4.2. Sample loading

•Load the samples using a gel loading pipette tip. During sample loading the

pipette tip should be 1 - 2 mm above the bottom of the well to minimise dilution of

the sample and to keep the sample as a tight layer.

•Avoid cross-contamination by overloading the sample wells.

•Fill unused wells with the equivalent volume of sample buffer to maintain uniform

electrical resistance across the gel.

4.4.3. Start of electrophoresis

•Close the safety lid firmly making sure that the electrical connectors form a good

contact.

•Connect the electrophoresis apparatus to the power pack and connect the power

pack to the mains supply, e. g. SERVA BluePower 500x4 (BP-500x4). Turn all

settings to zero before turning on the mains supply. Adjust the controls to the

desired settings. Follow manufacturers instructions.

4.4.4. End of run

•Turn the power supply settings to zero, turn off mains supply and disconnect the

power leads.

•Remove the safety lid, take out the internal gel running unit and discard the buffer.

1 2 3

4 5 6

DOMINIQUE DUTSCHER SAS

•Discard the buffer from the outer buffer tank. Rinse the chambers twice with

distilled water then dry the electrode connectors with tissue. Ensure that the

connectors are clean and dry before usage or storage (see chapter 4.2).

For further information about the SERVA BlueLine, please contact the technical

service of SERVA Electrophoresis GmbH in Heidelberg, phone Tel.: +49 (0)6221

13840-44.

DOMINIQUE DUTSCHER SAS

5. Recommended reagents for vertical electrophoresis

SERVA reagents for electrophoresis underly stringent quality and application control to

ensure best performance and results. We recommend the usage of SERVA electro-

phoresis reagents especially along with BlueLine electrophoresis instruments as the

quality of consumables is fine-tuned to the equipment (application tests).

Precast gels and Starter Kits

SERVAGel™ Gels for SDS PAGE

SERVAGel™ TG PRiME™ Starter Kit 43206

SERVAGel™ TG PRiME™ 8 % Tris-Glycine Gel 8 %

10 43261

SERVAGel™ TG PRiME™ 10 % Tris-Glycine Gel 10 % 12 43263

10 43264

SERVAGel™ TG PRiME™ 12 % Tris-Glycine Gel 12 % 12 43266

10 43267

SERVAGel™ TG PRiME™ 14 % Tris-Glycine Gel 14 % 12 43269

2D 43271

SERVAGel™ TG PRiME™ 4-12 % Tris-Glycine Gel 4-12 % 12 43273

10 43274

SERVAGel™ TG PRiME™ 4-20 % % Tris-Glycine Gel 4-20 % 12 43276

10 43277

SERVAGel™ TG PRiME™ 8-16 % % Tris-Glycine Gel 8-16 % 12 43279

2D 43281

SERVAGel™ TG 8 % Tris-Glycine Gel 8 %

(Available upon request, please inquire.) 12 43208

10 43209

SERVAGel™ TG 10 % Tris-Glycine Gel 10 %

(Available upon request, please inquire.) 12 43210

10 43211

SERVAGel™ TG 12 % Tris-Glycine Gel 12 %

(Available upon request, please inquire.)

12 43212

10 43213

2D 43226

SERVAGel™ TG 14 % Tris-Glycine Gel 14 %

(Available upon request, please inquire.)

2D 43227

SERVAGel™ TG 16 % Tris-Glycine Gel 16 %

(Available upon request, please inquire.) 12 43216

10 43217

SERVAGel™ TG 4-12 % Tris-Glycine Gel 4-12 %

(Available upon request, please inquire.) 12 43232

10 43238

SERVAGel™ TG 4-20 % % Tris-Glycine Gel 4-20 %

(Available upon request, please inquire.) 12 43230

10 43236

SERVAGel™ TG 8-16 % % Tris-Glycine Gel 8-16 %

(Available upon request, please inquire.)

10 43237

SERVAGel™ Neutral pH 7.4 12 43220

10 43222

SERVAGel™ Neutral pH 7.4 Gradient 12 43221

10 43223

SERVAGel™ Neutral HSE Starter Kit 12 43207

SERVAGel™ Neutral HSE

10 43246

2D 43247

DOMINIQUE DUTSCHER SAS

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