SCIEX CESI 8000 Plus Manuel utilisateur

CESI 8000 Plus High Performance

Separation-ESI Module

User Manual

RUO-IDV-05-3897-B

February 2019

AB Sciex LLC

500 Old Connecticut Path

Framingham, Massachusetts 01701

USA

This document is provided to customers who have purchased SCIEX equipment to use in the

operation of such SCIEX equipment. This document is copyright protected and any reproduction

of this document or any part of this document is strictly prohibited, except as SCIEX may

authorize in writing.

Software that may be described in this document is furnished under a license agreement. It is

against the law to copy, modify, or distribute the software on any medium, except as specifically

allowed in the license agreement. Furthermore, the license agreement may prohibit the software

from being disassembled, reverse engineered, or decompiled for any purpose. Warranties are as

stated therein.

Portions of this document may make reference to other manufacturers and/or their products,

which may contain parts whose names are registered as trademarks and/or function as

trademarks of their respective owners. Any such use is intended only to designate those

manufacturers' products as supplied by SCIEX for incorporation into its equipment and does not

imply any right and/or license to use or permit others to use such manufacturers' and/or their

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of its products and are SCIEX’s sole and exclusive representations, warranties, and obligations.

SCIEX makes no other warranty of any kind whatsoever, expressed or implied, including without

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For Research Use Only. Not for use in Diagnostic Procedures.

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AB SCIEX™ is being used under license.

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Contents

CHAPTER 1: Safety, Notices and Labels, 13

Overview, 13

Alerts for Warning, Caution, Important, and Note, 14

Instrument Safety Precautions, 15

Moving Parts or Sharp Objects, 16

Electrical Safety, 16

Laser Safety (for Optional Laser Device), 17

Class 1 Laser Caution Label, 17

Chemical Precautions, 17

Safety Symbols and Labels, 18

High Voltage Electric Shock Risk Symbol, 18

Attention Safety Symbol, 18

Sharp Object Label, 18

Mobile Cart Caution Label, 19

Cancer and Reproductive Harm Label, 19

RoHS Notices, 19

China RoHS Caution Label, 19

Other Instrument Labels, 20

Recycling Label (WEEE), 20

Disposal of Devices Containing Mercury Components, 21

CE Mark Label, 21

RCM Mark Label, 21

CSA Mark Label, 21

章 2: 安全、通知和标签 , 23

综述 , 23

警告、注意、重要事项以及注释的提示 , 24

仪器安全防护措施 , 25

活动部件或尖锐物 , 26

电气安全 , 26

激光安全（适用于可选激光设备） , 26

1 类激光小心标签 (1 级激光警告标签 ), 26

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化学品注意事项 , 27

安全符号和标签 , 27

高压电击危险符号 , 27

注意安全符号 , 27

尖锐物标签 , 28

流动车警告标签 , 28

致癌和生殖危害标签 , 28

RoHS 通知 , 28

中国 RoHS 警告标签 , 29

其他仪器标签 , 29

环保标签 (WEEE), 29

处理包含加汞组件的设备 , 30

CE 标志标签 , 30

RCM 标志标签 , 30

CSA 标识标签 , 30

CHAPITRE 3: Sécurité, Consignes et étiquettes, 31

Présentation, 31

Alertes « Avertissement, Mise en garde, Important et Remarque », 32

Précautions de sécurité pour l'instrument, 33

Pièces mobiles ou Objets tranchants, 34

Sécurité électrique, 34

Sécurité laser (pour les appareils avec laser en option), 35

Étiquette de mise en garde : produit laser de classe 1, 35

Précautions chimiques, 36

Symboles et étiquettes de sécurité, 36

Symbole « Haute tension, risque de choc électrique », 36

Symbole de sécurité Attention, 37

Étiquette Objet tranchant, 37

Étiquette de mise en garde chariot mobile, 37

Étiquette concernant les risques de cancer et de malformations

congénitales, 37

Consignes RoHS, 38

Étiquette de mise en garde RoHS (Chine), 38

Autres étiquettes de l'instrument,38

Étiquette de recyclage (WEEE), 39

Élimination des appareils contenant des composants au

mercure, 39

Étiquette marque CE, 39

Étiquette marque MRC, 40

Étiquette marque CSA, 40

CHAPTER 4: System Overview, 41

About This Manual, 41

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Optional Detectors, 41

Introduction, 42

Instrument, 43

Sample Handling System, 44

Syringe Pump, Power Supply, LEDs and Interlock, 46

Syringe Pump, 46

High Voltage (HV) Power Supply, 46

LED Indicators, 46

Cartridge and Sample Cover Interlocks, 47

OptiMS Cartridge, 48

CHAPTER 5: Integrating the CESI 8000 Plus System with a Mass

Spectrometer, 51

CESI-MS Integration Procedures, 51

CESI 8000 Plus System: Installing OptiMS Cartridge, 53

OptiMS Cartridge Installation, 53

Thermo Scientific Mass Spectrometer: Integration with the CESI 8000 Plus

System, 57

Physical Integration with the CESI 8000 Plus System, 57

OptiMS Cartridge Sprayer Tip Installation into a Thermo

Scientific NanoSpray II Ion Source, 59

Establishing Communication Between the CESI 8000 Plus

Controller and the Thermo Scientific Mass Spectrometer, 65

Aligning the CESI 8000 Plus System with a Thermo Scientific

Mass Spectrometer, 66

SCIEX TripleTOF®5600 Mass Spectrometer: Integration with the CESI 8000

Plus System, 71

Physical Connections with the CESI 8000 Plus System, 71

OptiMS Cartridge Sprayer Tip Installation into the SCIEX

Nanospray® III Source, 73

Establishing Communication Between the CESI 8000 Plus

Controller and the SCIEX Mass Spectrometer, 80

Aligning the CESI 8000 Plus System with the SCIEX TripleTOF®

5600 Mass Spectrometer, 81

CHAPTER 6: 32 Karat Software Overview, 87

Overview of 32 Karat Software, 87

System Administration, 87

Controller and Instrument Start Up, 88

Controller/Network Login, 88

License Key, 89

Demo Mode, 89

Launching 32 Karat Software, 89

Instrument Start Up, 89

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Instruments and Projects, 90

Configuration, 90

Online Versus Offline, 90

Using the Direct Control Window to Load the Cartridge and Samples, 91

TripleTOF®5600 Mass Spectrometer Using AAO, 93

Methods and Sequences, 94

Creating and Editing a Method, 95

Instrument Setup Window, 95

Instrument Setup — Initial Conditions, 95

Instrument Setup — Time Program, 97

Separate Dialog, 98

Rinse Dialog, 100

Inject Dialog, 100

Stop Data, 102

End, 102

Saving a Method, 102

Overview of Creating a Sequence, 103

Single Run and Sequence Runs, 103

Programming a Sequence, 104

Sequence Table, 108

Sequence Validation, 109

CHAPTER 7: CESI 8000 Software, 111

Overview of the CESI 8000 Software, 111

Launching the CESI 8000 Software, 112

Lock and Unlock, 114

About Dialog, 114

Help, 114

Users, 114

Run Application, 115

Application Selection, 115

Samples and Vials, 118

Samples/Vials Dialog,118

Insert Option, 120

Sequence Dialog, 121

Sequence Table Rows, 123

Fill Down Option, 127

Display Options, 128

Vial Legend, 129

Tray Detail View, 129

Vial Preview, 130

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Run Acquisition, 131

SCIEX TripleTOF®5600 Mass Spectrometer Using AAO, 133

Graph Options, 134

Sequence Transfer Overview, 135

Sequence Transfer, 135

Describe Sequence, 141

Sequence Verification, 144

Sequence Display Options, 144

CHAPTER 8: Using a SCIEX TripleTOF®5600 Mass Spectrometer with

the CESI 8000 Plus System, 147

Introduction, 147

Stock Reagents and Other Consumables Required, 148

Preparation of Reagent Solutions and Test Sample, 149

Preparation of 10% (v/v) HAc—Background Electrolyte

(BGE), 150

Preparation of 20% (v/v) Acetic Acid (HAc)—For LE pH

Adjustment, 150

Preparation of 200 mM LE (Leading Electrolyte) Buffer, 150

Reconstitution of 1 μM Beta-Galactosidase Digest Solution

(Beta-Galactosidase Stock Solution), 151

Preparation of Beta-Galactosidase Test Sample, 151

Preparation of Beta-Galactosidase for Auto-calibration, 152

Buffer Tray Setup, 152

Sample Tray Setup, 153

Procedure for Installation of Adapter and OptiMS Cartridge, 154

Methods for the CESI 8000 Plus System, 155

CESI-MS Beta-Galactosidase System Performance Test, 159

Capillary Conditioning, 162

Establishing a Stable Spray and Determining Optimum ESI Voltage, 165

Running CESI-MS, 172

Create a Sequence in the Analyst®TF Software, 173

Start a Sequence in the Analyst®TF Software, 176

Load Sample and Reagents on the CESI 8000 Plus System, 177

Sample Tray Setup, 177

Create a Sequence in the CESI 8000 Software,179

Start a Sequence in the CESI 8000 Software, 183

Automatic Rinse Feature, 184

Beta-Galactosidase Analysis, 185

CHAPTER 9: Using a Thermo Scientific Mass Spectrometer with the

CESI 8000 Plus System, 189

Introduction, 189

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Stock Reagents and Other Consumables Required, 190

Preparation of Reagent Solutions and Test Sample, 191

Preparation of 10% (v/v) Acetic Acid (HAc)—Background

Electrolyte (BGE), 191

Preparation of 20% (v/v) Acetic Acid (HAc) — For LE Buffer pH

Adjustment, 192

Preparation of 200 mM LE (Leading Electrolyte) Buffer, 192

Reconstitution of 1 μM Beta-Galactosidase Digest Solution

(Beta-Galactosidase Stock Solution), 193

Preparation of Beta-Galactosidase Test Sample, 193

Sample Tray Setup for System Performance Test, 193

Procedure for Installation of Adapter and OptiMS Cartridge, 194

Capillary Conditioning, 195

Buffer Tray Setup, 195

Capillary Conditioning Procedure, 196

Establishing a Stable Spray and Determining Optimum ESI Voltage, 198

Tuning, 202

Running CESI-MS, 203

Create a Method with the Xcalibur Software, 204

Create a Sequence with the Xcalibur Software, 207

Start a Sequence with the Xcalibur Software, 208

Load Sample and Reagents on the CESI 8000 System, 209

Create a Sequence in the CESI 8000 Software, 211

Automatic Rinse Feature, 212

Start a Sequence in the CESI 8000 Software, 213

Beta-Galactosidase Analysis, 214

CHAPTER 10: Shutdown Procedures and Decoupling Instruments, 217

Shutdown and Decoupling Overview, 217

Cartridge Removal and the CESI 8000 Plus System Shutdown, 217

Cartridge Removal and Instrument Shutdown, 217

Decoupling the CESI 8000 Plus System, 222

Decoupling from the Thermo Scientific Mass Spectrometer, 222

Decoupling from the SCIEX Mass Spectrometer, 225

APPENDIX A: Specifications, 229

CESI 8000 Plus System, 229

Validated Controller Configuration, 230

Cart Specifications, 230

Sample Temperature Control, 231

Capillary Temperature Control, 231

Pressure and Vacuum System, 231

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Detector Specifications, 232

UV Detector, 232

Laser Induced Fluorescence (LIF) Detector (Optional), 233

Photo Diode Array (PDA) Detector (Optional), 233

APPENDIX B: Nominal Network Configuration, 235

Overview, 235

Switching from the CESI 8000 Plus System to Different Mass Spectrometer

Systems, 236

Switching to the CESI 8000 Plus System from Different Mass Spectrometer

Systems, 236

Switching from a SCIEX Mass Spectrometer to Another Mass

Spectrometer, 237

Switching to a SCIEX Mass Spectrometer from Another Mass

Spectrometer, 239

Advanced Networking and Computer Configuration for the Analyst®TF 1.7

Software, 241

CE-MS Networking, 241

PC Configurations, 241

Network Sharing Setting for the Crossover Connection, 262

Setting the Mass Spectrometer Type, 264

Curtain Gas Patch Installation, 265

CE-MS Driver Installation, 267

Saving Network Configurations, 269

Analyst Hardware Profile Configuration, 270

To configure the Mass Spectrometer Hardware Profile, 270

Curtain Gas Setting and Verification, 276

Curtain Gas Setting, 276

Curtain Gas Verification, 278

APPENDIX C: TripleTOF®5600 Mass Spectrometer

Manual and Auto-Calibration Methods, 281

Overview, 281

Required Reagents, 281

Reagent and Sample Preparation, 281

Manual Calibration Before Auto-calibration, 282

Create a Reference Table for CE-MS Calibration, 282

Manual Calibration of the Mass Spectrometer, 285

Manual Calibration in TOF Mass Spectrometer Mode, 286

Manual Calibration in Product Ion Mode, 292

Methods for Auto-Calibration, 296

CE Method for Auto-Calibration, 296

MS Method for Auto-Calibration, 297

Executing Auto-Calibration, 300

Create a Sequence in the Analyst®TF Software, 300

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Start a Sequence in the Analyst®TF Software, 303

Load Sample and Reagents on the CESI 8000 System, 305

Sample Tray Setup, 305

Create a Sequence in the CESI 8000 Software, 306

Start a Sequence in the CESI 8000 Software, 310

Automatic Rinse Feature, 312

Auto-Calibration Data Analysis, 312

Troubleshooting Auto-Calibration Failure, 317

Auto-calibration Troubleshooting Table, 319

APPENDIX D: Auto Vial Increment Feature, 321

Overview, 321

Setting Auto Vial Increment on a Rinse Step, 321

Setting Auto Vial Increment on an Injection, 322

Setting Auto Vial Increment on a Separation Step, 324

Auto Vial Increment in a Method, 326

Reviewing Vial Positions before Running a Sequence, 327

APPENDIX E: Conductive Liquid Capillary Conditioning, 329

Conductive Liquid Capillary (CLC) Conditioning Procedure, 329

APPENDIX F: Using Vials and Hardware Maintenance, 331

Overview, 331

Using CESI-MS Vials and Caps, 332

CESI-MS Vials and Micro Vials, 332

Filling the Micro Vials, 333

Hardware Maintenance Procedures, 334

Capillary Cleaning Procedure (Short Term Storage), 334

Capillary Storage Procedure, 335

Removing and Re-installing OptiMS Interface Plate, 336

Opening Levers, Electrodes, and the Interface Block, 338

Clean Electrodes, Opening Levers, and the Interface Block, 341

Refilling Coolant, 343

Replacing Quad Rings, 344

Replacing Fuses, 346

APPENDIX G: Troubleshooting

and Mobile Cart Homing Procedure, 349

Overview, 349

Troubleshooting and Flow Diagrams, 349

Sample Preparation Considerations for Analysis with CESI-

MS, 350

Properties of a Method or Sequence Cannot be Modified or

Saved, 350

Blocked Sprayer Tip, 351

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