Santai Technologies SepaBean Manuel utilisateur
SepaBean machine
TM
Hardware Manual
Santai Technologies, Inc.
May 2017Release Date:
Table of Contents
Preface
Notices
01 Overview
1.1.Product Description
1.2.Product Features
1.3.Technical Parameters
1.4.Working Principle
02 Installation
03 Networking
04 Debugging
05 Operation
5.1.Method Setup
5.2.Run Setup
06 Instrument Maintenance
6.1.Change of Mobile Phase
6.2.Fuse Replacement
6.3.Hardware Maintenance
6.4.General Instrument Issues and Troubleshooting
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Preface
Thank you very much for purchasing the SepaBean machine, the flash
chromatography system by Santai Technologies, Inc (hereinafter called
Santai for short). In this manual, the operation and maintenance of the
SepaBean machine is described in detail.
For the proper use of the system and maximizing the output, user is
recommended to carefully read the instructions and safety measures as
good laboratory practice.
The SepaBean machine is an unique flash chromatography system which
provides learning and sharing tool to users. This instrument provides user
with a convenient and effective mobile operation interface, secured data
sharing, environmental-friendly column management system and
purification solution. The data-sharing feature helps user to obtain
information from the global knowledge database and share with each other
as well. The above mentioned features facilitate the improved efficiency of
research and development, secured data sharing, gathering of global
intelligence and enhanced authority over the separation and purification
for complex samples.
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Notices
The copyright and the right of interpretation of this manual and the related
software belong to Santai. Besides, the copyright of all trademarks and
product names mentioned in this manual are owned by this legally
registered company, Santai Technologies, Inc.
No part of this manual can be reproduced or transmitted in any form or by
any means without the written permission of Santai.
This system is covered by a standard limited warranty. The terms of
warranty are listed in this manual in detail.
All information in this manual, software, operating conditions, system
updates and control techniques are subject to change without prior notice
and does not represent a commitment of Santai.
The SepaBean machine and its components are subjected to various
patents filed in the People’s Republic of China and all over the world as
well.
Changes or modifications not solely approved by Santai could void the
user's authority to operate the equipment. The use of replacement,
consumable components, and accessories from other suppliers instead of
Santai is not recommended. Use such parts in SepaBean machine will be a
sole responsibility of user or related company.
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Safety Guidelines
Caution. As this machine deals with solvents,
silica gel and different chemical compounds, it is
recommended to use the safety goggles all the
time while working on this system.
Never open the pump tubing or joints when it is
under high backpressure. Tubing could rupture
due to high backpressure or valve may open
erroneously causing the spray of solvents. It is
recommended that the pump should be
depressurized before opening any of its joints.
Also it is recommended to use appropriate safety
gloves for different type of solvents.
Warning Instrument with High Voltages. Power
from the mainframe panel should be shut down
before opening the instrument.
Only the authorized Santai service personnel or
trained people by Santai can open the inside or
back panels of this machine.
Always use the provided power chord with the
system. If it is needed to use any other standard
power cord, please check if the power cord meets
the instrument voltage specifications and
requirements.
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Warning Electrical Grounding. The instrument
must be properly grounded to prevent damages
from lightening strike or electric leakage voltages.
Proper grounding can protect the operators from
an injury. Grounding resistance should be less
than 0.1 Ω. Also the leakage voltage should not
be more than 3V.
Warning High Temperature Surface. This symbol
indicates that the surface is too hot to touch with
hands. It may cause burns if not handled properly.
Warning Unknown Risks. User must be vigilant
when using the system.
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The production standards of this instrument are in line with the highest
safety regulation and quality standards of the country of origin. However it
does not rule out the possibility of human error or the instrument is not
operated in accordance with the Standard Operating Procedure (SOP).
SepaBean machines should be installed and stored in a GLP environment
and operated in the best suitable conditions. Any instrument issue or safety
issue must be informed to the dealer or Santai directly as soon as possible.
The instrument should be operated within the limits of pressure, flow,
solvent type and temperature as stated in the manual. The instrument is
equipped with multiple security systems and sensors. If the instrument is
operated in a non-compliant way, the system and sensors will fail to alarm
user about any possible risk. If unknown hazardous solvent or sample was
handled with, the personal protection gears should be included according
to the safe practices of lab. Laboratory reagents, which are corrosive,
flammable, toxic or any harmful characteristic should be handled under the
corresponding lab’s guidelines. User should pay attention to the safety
and protection issues.
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1.Overview
1.1.Product Description
SepaBean machine is a flash chromatography system and an
unconventional one. The utility of this machine is not only limited to flash
chromatography separation and purifications but also it is a learning and
sharing tool of global knowledge.
The SepaBean machine comprises of machine body, iPad, SepaBean
software and components related to the wireless communication. The
instrument has various modules integrated namely HPLC pump module, UV
detector module, fraction collector module, control module, alarm module,
cartridge arm and test module with some minor components. The default
communication device is iPad and the default operating system is iOS
(update to the latest version available).
The input of chromatography parameters, method setup, actual run and
data analysis with interpretation is realized through the iPad.
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Figure 1. SepaBean machine overview with components
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System Components Description
1.iPad and iPad holder – iPad is used to control the SepaBean machine
and it is installed on the holder which can be adjusted to operate the iPad
at eye level.
2.Machine Main Screen – Display the system status, schedules and other
information.
3.RFID Scanner – Check the RFID tag of the cartridge and display on the
main screen.
4.Test Tube Rack – Two racks accommodating 60 test tubes each, collecting
the liquid fractions and displaying the status on the built-in screen.
5.Cartridge RFID scanner – Identifies the type and size of cartridge with
RFID scanning and transfers the data to the software for method
development.
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Figure 2. SepaBean machine overview
with components
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Function A- Wipe
Function B- Wake
6.Leakage Drain – Drains the solvent leaked while installing the solid loader
or due to loosely installed cartridge.
7.Main Column Holder – For cartridge installation and fixation.
8.Column Arm Touch Keys – Make the arm move up and down when the
respective button is pressed.
9.Handle – Two handles on both sids allow an easy lift of the instrument.
10.Auxiliary column Holder – Pop up to hold various sizes of flash
cartridges, or put back by touch while not being used.
11.Solvent Bottle Trays – Hold various sizes of solvent bottles.
12.Emergency stop Stop Button – When pressed, it will stops the instrument
in emergency conditions.
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