Qiagen PyroMark Q24 Manuel utilisateur

Sample to Insight

Starting the instrument

Switch on the instrument using the power switch located at the rear. An

indicator light in the bottom left corner of the instrument screen lights

up when the instrument is on. The screen remains blank during startup,

which can take up to 1 minute.

Loading reagents and the PyroMark Q24 Plate

1. Fill a cartridge with the reagent volumes indicated by the PyroMark

Q24 Software and according to the instructions provided in the

PyroMark Gold Q24 Reagents Handbook.

2. If the instrument was already on, ensure that it is not performing a run

and then open the lid. An audible warning signal will alert you if the

lid is opened when it is unsafe to do so.

3. Open the cartridge gate and insert the cartridge with the label facing

you. Push the cartridge in fully and then push it down.

4. Ensure that the cartridge is properly inserted (a line should be visible

at the front) and close the gate.

5. Open the plate-holding frame and position the PyroMark Q24 Plate

with samples on the instrument heating block.

6. Close the plate-holding frame and the instrument lid.

Starting and monitoring a run

1. Insert the USB stick with the run files created with the PyroMark Q24

Software into the USB port at the front of the instrument.

2. Select Run in the main menu and press OK.

3. Use the scroll arrows to select the desired run file and press Select.

The instrument will begin dispensing reagents when all preset pressure

and temperature levels are reached (this may take several minutes).

PyroMark®Q24 Quick-Start Guide

This document briefly describes how to set up the PyroMark Q24 Instrument, start a Pyrosequencing®run, obtain run data,

and shut down the instrument. Before using the PyroMark Q24, it is essential that you read the PyroMark Q24 User Manual

carefully and pay particular attention to the safety information.

Location of cartridge gate.

Opening the plate-holding frame.

WARNING

Risk of personal injury and material damage.

To avoid contact with moving parts during operation,

the PyroMark Q24 Instrument must be operated with

the lid closed.

Do not touch the needles at the bottom of the cartridge.

Small particles and fibers may obstruct the needles.

1099605 12/2015

Trademarks: QIAGEN®, Sample to Insight®, Pyrogram®, PyroMark®, Pyrosequencing®(QIAGEN Group).

Ordering www.qiagen.com/contact Technical Support support.qiagen.com Website www.qiagen.com

For up-to-date licensing information and product-specific disclaimers, see the respective PyroMark Q24 User Manual. The

PyroMark Q24 User Manual is available at www.qiagen.com or can be requested from QIAGEN Technical Services or

your local distributor.

During a run, the instrument screen displays the Pyrogram®of the

selected well. Use the scroll arrows to view the Pyrogram of other wells.

After the run

1. When the instrument confirms that the run is finished and the run file

has been saved to the USB stick, press Close.

2. Remove the USB stick. If it was removed before the run finished, insert

the USB stick. Select Administration and then Copy Unsaved Runs.

3. Remove and discard the PyroMark Q24 Plate.

4. Remove the cartridge and discard remaining reagents. If the cartridge

is to be reused, follow the instructions in the PyroMark Q24 User

Manual to clean it properly.

5. Analyze the run with the PyroMark Q24 Software (see the PyroMark

Q24 Software User Guide).

Shutting down the instrument

1. Select Shutdown in the main menu and press OK.

2. When the message “It is now safe to turn off the instrument” appears

in the screen, switch off the instrument. The power switch is located at

the rear of the instrument.

3. Press the light button at the rear of the instrument and check that the

coolant level is visible in the window. If not, please contact QIAGEN

Technical Services.

4. If the instrument requires cleaning, follow the instructions provided in

the PyroMark Q24 User Manual.

Instrument screen indicating the end of a run.

Checking coolant levels.

coolant

level

light

button

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