ProteOn XPR36 Manuel utilisateur
ProteOn™XPR36
Protein Interaction Array System
Protein Interaction Analysis
User Manual
ProteOn XPR36
Protein
Interaction
Array System
Version 3.1 User Manual
™
™
iii
BIO-RAD TECHNICAL SUPPORT
Bio-Rad Technical Support in the United States is open Monday-Friday,
5:00 am to 5:00 pm, Pacific Standard Time.
Phone: 800-424-6723 (U.S. and Canada Only)
Fax: 510-741-5802
E-mail: lsg_techserv_us@bio-rad.com (USA and Canada Only)
Visit us on the Web at discover.bio-rad.com.
For international customers, contact your local Bio-Rad office. Contact
information for the Bio-Rad offices is available on the Web and on the back
cover of this manual.
Worldwide technical support is available on the Web at consult.bio-rad.com.
NOTICES
No part of this publication may be reproduced or transmitted in any form or by
any means, electronic or mechanical, including photocopying, recording, or
any information storage or retrieval system, without permission in writing from
Bio-Rad.
When running ProteOn Manager™ software in regions of the world where
decimals are expressed as commas, an error reading floating point values
might occur. Please use U.S. English numeric and date formats, as set in the
Control Panel, to avoid this problem. ProteOn Manager software has been
certified to run only using U.S. English.
TRADEMARKS
Adobe is a trademark of Adobe Systems Incorporated. Excel, Windows,
Windows Server, and Windows XP are trademarks of Microsoft Corporation.
The MySQL database is licensed from and owned by MySQL AB, a subsidiary
of Sun Microsystems, Inc. The program 7-Zip is used to extract database
files. It is licensed under a GNU LGPL license. You may find the source code
and other information at www.7-zip.org. NeutrAvidin is a trademark of Pierce
Biotechnology, Inc. Contrad is a trademark of Decon Laboratories. Kimwipes
is a trademark of Kimberly-Clark Corporation. Tween is a trademark of ICI
Americas Inc. HASP is a trademark of SafeNET Inc.
ProteOn XPR36 System |
iv
LIMITATIONS OF LIABILITY
Bio-Rad is not responsible for the misinterpretation of results obtained by
following the instructions in this manual. Whenever possible, you should
contact the Technical Support Department at Bio-Rad to discuss your results.
As with all scientific studies, we recommend that you repeat your experiment
at least once before making any significant conclusions for presentation or
publication.
Copyright © 2011 by Bio-Rad Laboratories, Inc. All rights reserved.
REFERENCES
Antibody characterization and development using the ProteOn™ XPR36
protein interaction array system, Bio-Rad Bulletin 5412 (2007)
Bravman T et al. (2006). Exploring 'One-shot' Kinetics™ and small-molecule
analysis using the ProteOn™ XPR36 array biosensor, Analytical Biochemistry,
Vol. 358, Issue 2, pp. 281–288.
Bravman T et al. (2007). Screening, ranking, and epitope mapping of anti-
human IL–9 supernatants, Bio-Rad Bulletin 5540.
Bravman T et al. (2008). The ProteOn™ XPR36 array system – high throughput
kinetic binding analysis of biomolecular interactions, Cellular and Molecular
Bioengineering, Vol. 1, pp. 216–228.
Bronner V et al. (2010). Rapid assay development and optimization for small
molecule drug discovery, Bio-Rad Bulletin 5797.
Bronner V et al. (2009). Rapid screening and selection of optimal antibody
capturing agents using the ProteOn™ XPR36 protein interaction array system,
Bio-Rad Bulletin 5820.
Bronner V et al. (2010). Therapeutic antibodies: Discovery and development
using the ProteOn XPR36 biosensor interaction array system, Analytical
Biochemistry, Vol. 406, Issue 2, pp. 147–156.
Bronner V et al. (2006). Rapid and efficient determination of kinetic rate
constants using the ProteOn™ XPR36 protein interaction array system, Bio-
Rad Bulletin 3172.
Bronner V et al. (2006). Mechanisms of protein-protein binding: double mutant
cycle analysis using the ProteOn™ XPR36 system, Bio-Rad Bulletin 5358.
Bronner V et al. (2006). Rapid and detailed analysis of multiple antigen-
antibody pairs using the ProteOn™ XPR36 protein interaction array system,
Bio-Rad Bulletin 5360.
Bronner V et al. (2006). Rapid optimization of immobilization and binding
conditions for kinetic analysis of protein-protein interactions using the
ProteOn™ XPR36 protein interaction array system, Bio-Rad Bulletin 5367.
v
Bronner V et al. (2006). Analysis of multiple protein-protein interactions using
the ProteOn™ XPR36 protein interaction array system, Bio-Rad Bulletin 5368.
Cohen S et al. (2010). Applications of the ProteOn™ NLC sensor chip:
antibody-antigen, DNA-protein, and protein-protein interactions, Bio-Rad
Bulletin 5449.
Moriarty L. (2010). Guide to ligand immobilization on the ProteOn™ XPR36
system, Bio-Rad Bulletin 5821.
Nahshol O et al. (2008). Parallel kinetic analysis and affinity determination of
hundreds of monoclonal antibodies using the ProteOn™ XPR36, Analytical
Biochemistry, Vol. 383, Issue 2, pp. 52–60.
Stephen A et al. (2009). Determining the binding kinetics of HIV–1
nucleocapsid protein to six densities of oligonucleotide using the ProteOn™
XPR36 protein interaction array system, Bio-Rad Bulletin 5846.
Tabul M et al. (2010). Rapid, high-throughput screening of protein kinase
inhibitors using the ProteOn™ XPR36 protein interaction array system, Bio-
Rad Bulletin 5965.
Turner B et al. (2008). Applications of the ProteOn™ GLH sensor chip:
interactions between proteins and small molecules, Bio-Rad Bulletin 5679.
SAFETY
Your safety is important to us. The ProteOn™ XPR36 protein interaction array
system contains components that, if handled improperly, are potentially
harmful. The protection provided by the equipment can be compromised or
the warranty voided if the equipment is used in a manner not specified by
Bio-Rad Laboratories. Bio-Rad recommends that all system users become
familiar with the comprehensive operating procedures and safety information
in this manual. This information alerts you to potential hazards. Please review
all safety information and adhere to standard laboratory safety practices.
WARNING NOTICES USED IN THIS MANUAL
The triangle warning symbol indicates the possibility of personal
injury or damage to the equipment if instructions are not followed.
ELECTRICAL SAFETY
The instrument must be connected only to an approved power
source. Use a grounded socket that meets power requirements. For
more information, refer to Appendix B Specifications and
Requirements on page 209.
Do not attempt to clean the instrument’s electrical components.
ProteOn XPR36 System |
vi
In the event of a fluid leak, turn off all power to the system and disconnect all
power cords. Contact Bio-Rad Laboratories for further information.
UNINTERRUPTIBLE POWER SUPPLY
An uninterruptible power supply is recommended to protect the ProteOn
XPR36 from an unstable main power source, harmful spikes, surges or total
blackouts.
This table contains minimum specifications for an uninterruptible power
supply that can be used with the ProteOn instrument.
MECHANICAL SAFETY
Use caution when handling the needle tips; they are sharp.
Keep fingers clear of the autosampler during operation.
Shut down the instrument and turn off the power before handling
the autosampler arm, needle holder, or needles.
BIOLOGICAL SAFETY
All human and animal samples may contain hazardous infectious
agents. Follow appropriate biosafety procedures when handling
these products and their containers.
Observe all local, state, and federal biohazard handling
regulations when disposing of biohazardous waste material.
Specification 120 VAC 230 VAC
Output power capacity 1,500 VA 1,500 VA
Nominal input voltage 120 V 230 V
Backup time Minimum 15 min at full load Minimum 15 min at full load
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PERSONAL SAFETY
The ProteOn XPR36 instrument weighs 187 lb (85 kg).
Follow all personal safety precautions when moving the
instrument.
If you must lift the instrument, use proper lifting techniques. Have two people
lift the instrument, and use the lifting points indicated below:.
Caution: Bio-Rad strongly recommends that you keep your
boxes for future moving and hire a professional service to safely
move the instrument.
ProteOn XPR36 System |
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