ProteOn XPR36 Manuel utilisateur

ProteOn™XPR36

Protein Interaction Array System

Protein Interaction Analysis

User Manual

ProteOn XPR36

Protein

Interaction

Array System

Version 3.1 User Manual

™

iii

BIO-RAD TECHNICAL SUPPORT

Bio-Rad Technical Support in the United States is open Monday-Friday,

5:00 am to 5:00 pm, Pacific Standard Time.

Phone: 800-424-6723 (U.S. and Canada Only)

Fax: 510-741-5802

E-mail: lsg_techserv_us@bio-rad.com (USA and Canada Only)

Visit us on the Web at discover.bio-rad.com.

For international customers, contact your local Bio-Rad office. Contact

information for the Bio-Rad offices is available on the Web and on the back

cover of this manual.

Worldwide technical support is available on the Web at consult.bio-rad.com.

NOTICES

No part of this publication may be reproduced or transmitted in any form or by

any means, electronic or mechanical, including photocopying, recording, or

any information storage or retrieval system, without permission in writing from

Bio-Rad.

When running ProteOn Manager™ software in regions of the world where

decimals are expressed as commas, an error reading floating point values

might occur. Please use U.S. English numeric and date formats, as set in the

Control Panel, to avoid this problem. ProteOn Manager software has been

certified to run only using U.S. English.

TRADEMARKS

Adobe is a trademark of Adobe Systems Incorporated. Excel, Windows,

Windows Server, and Windows XP are trademarks of Microsoft Corporation.

The MySQL database is licensed from and owned by MySQL AB, a subsidiary

of Sun Microsystems, Inc. The program 7-Zip is used to extract database

files. It is licensed under a GNU LGPL license. You may find the source code

and other information at www.7-zip.org. NeutrAvidin is a trademark of Pierce

Biotechnology, Inc. Contrad is a trademark of Decon Laboratories. Kimwipes

is a trademark of Kimberly-Clark Corporation. Tween is a trademark of ICI

Americas Inc. HASP is a trademark of SafeNET Inc.

ProteOn XPR36 System |

LIMITATIONS OF LIABILITY

Bio-Rad is not responsible for the misinterpretation of results obtained by

following the instructions in this manual. Whenever possible, you should

contact the Technical Support Department at Bio-Rad to discuss your results.

As with all scientific studies, we recommend that you repeat your experiment

at least once before making any significant conclusions for presentation or

publication.

REFERENCES

Antibody characterization and development using the ProteOn™ XPR36

protein interaction array system, Bio-Rad Bulletin 5412 (2007)

Bravman T et al. (2006). Exploring 'One-shot' Kinetics™ and small-molecule

analysis using the ProteOn™ XPR36 array biosensor, Analytical Biochemistry,

Vol. 358, Issue 2, pp. 281–288.

Bravman T et al. (2007). Screening, ranking, and epitope mapping of anti-

human IL–9 supernatants, Bio-Rad Bulletin 5540.

Bravman T et al. (2008). The ProteOn™ XPR36 array system – high throughput

kinetic binding analysis of biomolecular interactions, Cellular and Molecular

Bioengineering, Vol. 1, pp. 216–228.

Bronner V et al. (2010). Rapid assay development and optimization for small

molecule drug discovery, Bio-Rad Bulletin 5797.

Bronner V et al. (2009). Rapid screening and selection of optimal antibody

capturing agents using the ProteOn™ XPR36 protein interaction array system,

Bio-Rad Bulletin 5820.

Bronner V et al. (2010). Therapeutic antibodies: Discovery and development

using the ProteOn XPR36 biosensor interaction array system, Analytical

Biochemistry, Vol. 406, Issue 2, pp. 147–156.

Bronner V et al. (2006). Rapid and efficient determination of kinetic rate

constants using the ProteOn™ XPR36 protein interaction array system, Bio-

Rad Bulletin 3172.

Bronner V et al. (2006). Mechanisms of protein-protein binding: double mutant

cycle analysis using the ProteOn™ XPR36 system, Bio-Rad Bulletin 5358.

Bronner V et al. (2006). Rapid and detailed analysis of multiple antigen-

antibody pairs using the ProteOn™ XPR36 protein interaction array system,

Bio-Rad Bulletin 5360.

Bronner V et al. (2006). Rapid optimization of immobilization and binding

conditions for kinetic analysis of protein-protein interactions using the

ProteOn™ XPR36 protein interaction array system, Bio-Rad Bulletin 5367.

Bronner V et al. (2006). Analysis of multiple protein-protein interactions using

the ProteOn™ XPR36 protein interaction array system, Bio-Rad Bulletin 5368.

Cohen S et al. (2010). Applications of the ProteOn™ NLC sensor chip:

antibody-antigen, DNA-protein, and protein-protein interactions, Bio-Rad

Bulletin 5449.

Moriarty L. (2010). Guide to ligand immobilization on the ProteOn™ XPR36

system, Bio-Rad Bulletin 5821.

Nahshol O et al. (2008). Parallel kinetic analysis and affinity determination of

hundreds of monoclonal antibodies using the ProteOn™ XPR36, Analytical

Biochemistry, Vol. 383, Issue 2, pp. 52–60.

Stephen A et al. (2009). Determining the binding kinetics of HIV–1

nucleocapsid protein to six densities of oligonucleotide using the ProteOn™

XPR36 protein interaction array system, Bio-Rad Bulletin 5846.

Tabul M et al. (2010). Rapid, high-throughput screening of protein kinase

inhibitors using the ProteOn™ XPR36 protein interaction array system, Bio-

Rad Bulletin 5965.

Turner B et al. (2008). Applications of the ProteOn™ GLH sensor chip:

interactions between proteins and small molecules, Bio-Rad Bulletin 5679.

SAFETY

Your safety is important to us. The ProteOn™ XPR36 protein interaction array

system contains components that, if handled improperly, are potentially

harmful. The protection provided by the equipment can be compromised or

the warranty voided if the equipment is used in a manner not specified by

Bio-Rad Laboratories. Bio-Rad recommends that all system users become

familiar with the comprehensive operating procedures and safety information

in this manual. This information alerts you to potential hazards. Please review

all safety information and adhere to standard laboratory safety practices.

WARNING NOTICES USED IN THIS MANUAL

The triangle warning symbol indicates the possibility of personal

injury or damage to the equipment if instructions are not followed.

ELECTRICAL SAFETY

The instrument must be connected only to an approved power

source. Use a grounded socket that meets power requirements. For

more information, refer to Appendix B Specifications and

Requirements on page 209.

Do not attempt to clean the instrument’s electrical components.

ProteOn XPR36 System |

In the event of a fluid leak, turn off all power to the system and disconnect all

power cords. Contact Bio-Rad Laboratories for further information.

UNINTERRUPTIBLE POWER SUPPLY

An uninterruptible power supply is recommended to protect the ProteOn

XPR36 from an unstable main power source, harmful spikes, surges or total

blackouts.

This table contains minimum specifications for an uninterruptible power

supply that can be used with the ProteOn instrument.

MECHANICAL SAFETY

Use caution when handling the needle tips; they are sharp.

Keep fingers clear of the autosampler during operation.

Shut down the instrument and turn off the power before handling

the autosampler arm, needle holder, or needles.

BIOLOGICAL SAFETY

All human and animal samples may contain hazardous infectious

agents. Follow appropriate biosafety procedures when handling

these products and their containers.

Observe all local, state, and federal biohazard handling

regulations when disposing of biohazardous waste material.

Specification 120 VAC 230 VAC

Output power capacity 1,500 VA 1,500 VA

Nominal input voltage 120 V 230 V

Backup time Minimum 15 min at full load Minimum 15 min at full load

vii

PERSONAL SAFETY

The ProteOn XPR36 instrument weighs 187 lb (85 kg).

Follow all personal safety precautions when moving the

instrument.

If you must lift the instrument, use proper lifting techniques. Have two people

lift the instrument, and use the lifting points indicated below:.

Caution: Bio-Rad strongly recommends that you keep your

boxes for future moving and hire a professional service to safely

move the instrument.

ProteOn XPR36 System |

viii

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