Pop-Bio VU-C Manuel utilisateur
VÜ IMAGING MANUAL V2.0
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Vü Imaging Systems
User Manual
VÜ IMAGING MANUAL V2.0
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Copyright
This document may not be reproduced or copied in any way, stored electronically or translated into any
language without the permission of Pop-Bio Ltd
Trademarks/Patents
Trademarks and Patents are registered by Pop-Bio Ltd. All other trademarks or registered trademarks are the
property of their respective holders. Specification and software are subject to change without notice.
Disclaimer
The contents of this manual have been checked for accuracy and every effort has been made to ensure there
are no errors. Pop-Bio Ltd makes no guarantee as to the accuracy of this document and reserves the right to
make changes to the contents without prior notice.
Pop-Bio Ltd
October 2018
VÜ IMAGING MANUAL V2.0
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Safety Precautions
Before using the Vü Imaging System read the following safety precautions and this manual. Proper operation
of this system and its accessories with help to prevent injuries or damage to the equipment or users
Symbols used throughout this manual
Power Supply
Use only the power supply provided by Pop-Bio
Ensure the mains power to all devices are switched off before connecting or
disconnecting cables
Ensure mains plug is fully inserted into socket to avoid ‘arching’
If the device is not being used for long periods unplug it from the mains socket
Moving the device
Always select the ‘park’ position before moving the device
Do not drop the device
Place the device on a flat, dry surface
Avoid placing the device where it can get wet
Place the device away from any heat sources and direct sunlight
Maintenance and Service
Ensure the device is unplugged from the mains before conducting any servicing or
maintenance
This symbol indicates CAUTION. Not following instructions could lead to injury or
damage to equipment or users.
This symbol indicates WARNING Not following instructions could lead to serious
injury or death to users.
VÜ IMAGING MANUAL V2.0
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Use only parts supplied by Pop-Bio Imaging
Only suitably qualified persons should conduct any maintenance or service operation
Illumination
The Blue LED light present a low risk and in normal operation should not be visible to the
user
The UV tube does present a UV hazard however in normal operation should not be visible
to the user
Avoid attempting to use the system in anyway where Blue of UV light is visible to the
user.
VÜ IMAGING MANUAL V2.0
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Contents
Page 3 Safety Precautions
Page 6 The Vü Imaging system
Page 7 System requirments
Page 8 Getting to know your system
Page 9 Setting up your system
Page 10 Installation and installing the Vü software
Page 13 Using the Vü-F
Page 16 Using the Vü-C
Page 19 How to get the best images from your Vü system
Page 24 Understanding the Vü software
Page 34 Taking care of your Vü
Page 35 Maintenance and settings
Page 41 Specifications
VÜ IMAGING MANUAL V2.0
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The Vü Imaging system
The Vü imaging system is available in two configurations.
Vü-C is for chemiluminescence applications. The unit has built in RGB LED’s which are used for illumination of
marker lanes on a blot. No other lighting is available. The Vü-C is used exclusively for chemiluminescent
western blot applications
Vü-F is for Fluorescence application. The unit has built in blue LED’s and a 302nm UV tube. Blue light is used
for a wide range of ‘safe’ dye application. The UV is typically used for illuminating Etbr stained DNA gels. A
white light converter is used to created transmitted white light.
This manual covers both systems
Key features of the Vü systems
•Highly sensitive sensor technology able to acquire low light level faint bands and blots
•A patented acquisition [applied for] system which automatically controls the sensor head unit and
processes the acquired image.
•Simple interface to select method of operation and location of saved images.
•Very small foot print taking up minimal bench space
•Easy to install, operate and maintain.
VÜ IMAGING MANUAL V2.0
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System requirements
The Vü system can be operated as follows:
The Vü system can be connected to a network either by ethernet cable or a WiFi adaptor. There is an ethernet
port on the rear of the system and spare Wifi slots for a WiFi adaptor.
It is also possible to connect the system directly to a computer using the ethernet cable. This is best when a
stand-alone installation is required and is probably the most common setup.
The computer should have the following specification.
•PC using Windows 10 or higher
•200 MB HDD or higher
•512 MB RAM or higher
•Ethernet connection
•USB for software updates
See the separate software installation instructions for connection to a network or stand-alone computer.
VÜ IMAGING MANUAL V2.0
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Getting to know your system
Front View
Rear View
VÜ IMAGING MANUAL V2.0
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Setting up the system
Unpacking the system
Carefully remove the unit from its box –remove any packing pieces that are used
Locate the following items included with the system.
Vu-C
•Black plastic plate use to place over the blot
•Power supply
•Appropriate power cable
•Ethernet cable [ yellow]
•Plastic tray for blot
Vu-F
•Black tray for blue light applications
•Plastic tray for gels
•Power supply
•Appropriate power cable
•Ethernet cable [blue]
•White light conversion screen
•UV transmitting glass spacer for use with very thin gels
Also supplied with the system[s] is a folder containing software installation instructions, memory stick with
Vü software and this instructions manual, Analysis software licence information.
Check for any damage to the unit or packing. Report any damage in the first instance to the shipper and then
to Pop-Bio for further instruction or advice. Retain all packing material if the unit needs to be shipped later.
If there are any shortages contact Pop-Bio for instruction or advice
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Installing the unit
Place the unit on a clean, flat, dry surface with the front drawer being easy to reach
Locate the 4 pin socket on the rear of the unit and plug the lead from the power supply into this.
Using the power cable supplied connect the power supply to a suitable mains outlet. [do not switch on the
mains socket at this time]
Plug the ethernet cable into the appropriate socket on the rear of the unit.
Plug the other end of the ethernet lead into a stand-alone computer. Depending on the type of computer you
have you may need to use an adaptor [ not supplied] to connect the ethernet lead to a suitable port.
Switch on your computer first.
Software Installation
Finding the Network name
The initial set up of the system requires that you use a stand-alone computer connected to the system. This is
the most common set up however later you may wish to connect the system to work on a network with each
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