PerkinElmer OPERETTA Manuel utilisateur

OPERETTA®

Technical Manual

Release History

Part Number Release Publication Date

HH12940105 I 08.04.2011

Service Contact

Information: http://www.perkinelmer.com

Portal: http://evoportal.perkinelmer.com

Notices

The information contained in this document is subject to change without notice.

Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no

warranty of any kind with regard to this document, including, but not limited to, the

implied warranties of merchantability and fitness for a particular purpose.

PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in

connection with furnishing, performance or use of this material.

This document contains proprietary information that is protected by copyright. All rights are

reserved. No part of this publication may be reproduced in any form whatsoever or translated into

any language without the prior, written permission of PerkinElmer, Inc.

Printed in the UK.

Trademarks

Registered names, trademarks, etc. used in this document, even when not specifically marked as

such, are protected by law.

PerkinElmer is a registered trademark of PerkinElmer, Inc.

Operetta is a registered trademark of PerkinElmer, Inc.

Harmony is a registered trademark of PerkinElmer, Inc.

plate::handler is a trademark of PerkinElmer, Inc.

plate::works is a trademark of PerkinElmer, Inc.

Patents

This product or its use may be covered by one or more of the following patents:

DE 103 33326, EP 1 646 902, US 7 424 401, EP 1 449 081, DE 101 23027, EP 1 386 189 ,

US 7 304 793, CA 2 282 658, AU 730 100, EP 0 983 498, JP 3 683 591, US 6 620 591,

US 6 902 883, US 6 573 039, US 5 989 835, US 6 671 624, US 6 759 206, CA 2 328 194,

EP 1 095 277, JP 3 466 568, US 6 416 959, EP 1155304, CA 2,362,117, JP 3576491,

US 6 727 071, US 6 875 578, US 6 716 588, EP 1 348 124, US 6 917 884, US 6 365 367,

EP 1 314 980, EP 1 203 214, CA 2 381 344, EP 1 368 653, EP 1 368 689

Contents

1 System Safety 5

1.1 Purpose-Oriented Application 6

1.2 Signal Words and Symbols 7

1.3 Indication of Hazards and Danger Zones 8

1.3.1 Operating and Maintenance Staff 8

1.3.2 Mechanical Hazard 8

1.3.3 Process Liquids or Substances 9

1.3.4 Light Sources 10

1.3.5 Carbon Dioxide (only with TCO Upgrade) 11

1.3.6 Electric Shock 12

1.3.7 Disconnecting from Mains Supply 12

1.3.8 Spilling of Liquids 13

1.3.9 Cleaning 13

1.3.10 Ventilation 13

1.3.11 Disposal 13

1.4 Warning Signs 14

1.5 Protection against Harmful Interference 14

1.6 Warranty Information 14

1.7 Conformity 16

1.8 Service Schedule 17

1.9 Setup Requirements 17

2 Hardware Description 19

2.1 Introduction 20

2.1.1 Instrument Features 20

2.2 Operetta Device 21

2.2.1 Right Side 21

2.2.2 Left Side 22

2.2.3 Front Side 22

2.2.4 Instrument Status 24

2.2.5 Sample Chamber 25

2.2.6 CCD Camera 26

2.3 Temperature and CO2 Option (TCO) 27

2.3.1 Overview 27

2.3.2 Live Cell Chamber 28

2.3.3 CO2 Supply 29

2.4 Control PC (Harmony PC) 31

2.5 Xenon Light Source 31

2.6 Additional Upgrades 32

3 Retooling and Maintenance 33

3.1 Retooling 34

3.1.1 Objective Lens 34

3.1.2 Correction Collar 36

3.1.3 Emission Filter 38

3.2 User Maintenance 40

3.2.1 Cermax® Xenon Lamp 40

3.2.2 Cleaning 41

3.2.3 Replacing a Blown Fuse 42

3.2.4 Transportation 43

3.3 Maintenance by PerkinElmer Service 43

4 IT Policy 44

4.1 Network Integration 45

4.1.1 Overview 45

4.1.2 Simple Integration 46

4.1.3 Alternative Solutions 47

4.1.4 Automation Upgrade (plate::handler II) 49

4.2 Microsoft Windows Security Updates / Service Packs 50

4.3 Requirements for Harmony Installation on an Office PC 51

4.4 Computer Systems 52

4.4.1 Network 52

4.4.2 Hardware 52

4.4.3 Operating System, Software & Software Applications 52

4.4.4 Data 53

4.4.5 Back Up 53

4.4.6 Security Settings & Anti-Virus Protection 53

4.5 Computer Users 53

4.6 Remote Support 54

5 Technical Data 55

5.1 Optical Configuration 56

5.2 Basic Components 58

5.2.1 Detection 58

5.2.2 Light Sources 58

5.3 Specifications 59

5.3.1 Objective Lenses 59

5.3.2 Sample Carrier 59

5.3.3 Power Supply 60

5.4 Consumables 60

1 System Safety

Operetta Technical Manual 5

1.1 Purpose-Oriented Application

Caution!

Use only as intended.

The Operetta system, including the original accessories may only be

used in conjunction with the methods described in this manual.

PerkinElmer does not assume any liability for any other applications

or procedures, including use of individual subassemblies or

components for other purposes.

The manufacturer does not assume any liability for any other kind of

application, including individual subassemblies and the addition of

individual components. This exclusion of liability also applies to all

service or repair work which was not carried out by authorized

PerkinElmer service personnel.

Intended Use and Biosafety

The Operetta is an automated fluorescence imaging reader for usage in biological-

medical and pre-clinical research (biosafety levels 1 and 2, see also section 1.3.3

"Process Liquids or Substances", page 9). Due to its technical design the Operetta is

particularly suitable for cellular applications. The instrument is not designed to be used

in the clinical or medical-diagnostical area.

Exclusion of Medical Applications

The Operetta is not approved for use in human or veterinary medical applications,

including but not limited to in vivo or in vitro diagnostics. PerkinElmer explicitly

excludes such applications of the Operetta, and will not assume liability for any

unauthorized use of the instrument in these areas.

Exclusion of Liability for Measurement Results

PerkinElmer does not assume any liability for the correctness of measurement results

obtained with the PerkinElmer Operetta system nor for conclusions based on these

measurement results.

1 System Safety

6 Operetta Technical Manual

1.2 Signal Words and Symbols

The following signal words are used in this manual:

Danger!

Indicates a hazardous situation which, if not avoided, can result in death

or irreversible injury.

Warning!

Indicates a hazardous situation which, if not avoided, can result in

severe but normally reversible injury.

Caution!

Indicates a hazardous situation which, if not avoided, can result in pain

or minor injury.

Notice

Failure to observe may result in invalid measurement results or damage

of the instrument.

Specific symbols are used which show you the type of hazard:

Biohazard!

Risk of crushing!

Failure to observe may result in injury or damage to the system.

Automatic start!

Parts can suddenly start moving.

Hot Surface!

Concentrated laser beam!

Use of controls or adjustments or performance of procedures other than

those specified herein may result in hazardous radiation exposure.

Electrical shock!

Direct and indirect electrical contact.

1 System Safety

Operetta Technical Manual 7

UV/Vis Light!

1.3 Indication of Hazards and Danger Zones

Before operating the Operetta system for the first time please read this manual

carefully to avoid incorrect operation of the Operetta system. Also read the safety

instructions given in the separate manual of the Xenon lamp.

1.3.1 Operating and Maintenance Staff

lThe Operetta may only be operated by qualified staff who have been specially

trained and are familiar with the contents of this manual and the operating

instructions of the device.

lThe system must only be operated when it is in a fully assembled and installed

condition.

lUnauthorized intervention or tampering inside the machine is prohibited.

lCorrective maintenance work and service may only be performed by the

PerkinElmer service. Operational and maintenance procedures described in this

manual are excepted and may be carried out by the user.

lThe manufacturer can accept no liability for this product if the provisions of this

documentation are not complied with.

lAlways wear your personal safety equipment, i.e. safety goggles and laboratory

coat.

1.3.2 Mechanical Hazard

Warning!

Moving scanning table – Risk of crushing

The Operetta scanning table operates automatically. It can suddenly

start moving.

lDo not manipulate the interlocks of the lid or the protective

doors and shields (automation upgrade).

lDo not reach into the danger area of the scanning table.

lDo not reach inside the Operetta through the scanning table

flap.

The Operetta has a moving scanning table. This table is accessible if driven out of the

device for loading of new sample carriers (only for automation upgrade). An interlock

switch stops driving of the table motors immediately as soon as the Operetta's lid is

opened.

1 System Safety

8 Operetta Technical Manual

1.3.3 Process Liquids or Substances

Danger! Biohazard!

This device is designed for analyzing biological substances at

biosafety level 1 and 2 (BSL-1 or BSL-2).

lDo not use more hazardous substances due to limited

possibilities of decontamination.

lObserve the safety instructions for the substances used.

lObserve the instructions for decontamination and cleaning.

lAlways make sure to comply with national and international

laboratory safety instructions.

lAlways wear your personnel safety equipment.

The processing of samples on the Operetta covers only probes at biosafety level 1 and

2 (BSL-1 or BSL-2, CDC http://www.cdc.gov/od/ohs/biosfty/bmbl4/bmbl4s3.htm).

BSL-1 includes “well-characterized agents not known to consistently cause disease in

healthy adult humans, and of minimal potential hazard to laboratory personnel and the

environment”. BSL-2 is “suitable for work involving agents of moderate potential

hazard to personnel and the environment”.

All procedures performed on the Operetta should be done carefully to minimize the

creation of splashes. Biological substances higher than BSL-2 may not be used. As

though developed for easy cleaning and low internal contamination, a sufficient

sterilization and decontamination especially of internal mechanics and electronics

cannot be guaranteed!

It is fully within the responsibility of the operator to ensure that all appropriate

measures are taken to ensure that no substance processed by Operetta exposes any

hazard to the system, personnel or the environment. It is also within the responsibility

of the user to attach a biohazard label as soon as potential infective substances are

used.

As supplied, Operetta does not provide any particular protection against the hazards

evolving from aggressive chemicals, biological active substances or radioactive

substances.

1 System Safety

Operetta Technical Manual 9

1.3.4 Light Sources

The Operetta is equipped with a class 3B autofocus laser and a high voltage Xenon

lamp.

Danger!

Laser radiation - Eye injury

Class 3B invisible laser radiation may be present inside the

Operetta`s protective housing during operation. Since the housing’s

access doors are protected by laser safety interlocks, no harmful

radiation will become accessible when the doors are opened for

setup and maintenance.

lDo not defeat the laser safety interlock!

lDo not remove any housing components!

lAVOID DIRECT EXPOSURE TO BEAM!

lSwitch off the Operetta system before starting any permitted

retooling and maintenance tasks.

lService tasks that require access to the instrument while the

interlocks are defeated may only be carried out by qualified

service personnel. Service personnel must observe the

instructions of the separate Service Manual.

Danger!

Scattered laser radiation (only automated version)!

Scattered laser radiation may occur if the automation sample carrier

access door on the right for sample carrier input / output is opened

during operation.

lDo not open the sample carrier access door!

Danger!

Ultraviolet light - Eye injury

Ultraviolet light (visible and invisible) from the Xenon lamp is present

inside the Operetta. Since the housing’s access doors are protected

by laser safety interlocks, no harmful radiation will become

accessible when the doors are opened for setup and maintenance.

lDo not defeat the laser safety interlock.

1 System Safety

10 Operetta Technical Manual

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