Optics11 Piuma Nanoindenter Manuel utilisateur

PIUMA NANOINDENTER
USER MANUAL

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CONTACT INFORMATION
Optics11
+31 20 598 7917
www.optics11.com
VISITING ADRESS
Optics11
VU University Campus
W&N Building room O-236
De Boelelaan 1081
1081 HV Amsterdam
The Netherlands
SHIPPING ADRESS
Optics11
De Boelelaan 1081
1081 HV Amsterdam
The Netherlands
COMPANY INFORMATION
Optics11 B.V.
KvK/CC: 52469417
VAT: NL850459734B01
Amsterdam, NL
Please see our website
for more information
about our products.
www.optics11.com

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CONTENTS
Revision history 4
Safety 5
Introduction 6
1. Installation 7
2. Preparing the setup for measurement 10
3. Using the Piuma Nanoindenter 22
4. Optimizing instrument stability and sample
mounting 33
5. Working principle of the Piuma Nanoindenter
instrument 38
6. Description of the Piuma software 44
7. The OP1550 interferometer 57
8. FAQ & troubleshoot 61

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REVISION HISTORY
Version 2.0 New document December 6, 2016 Ernst Breel
Version 2.1 Update March 14, 2017 Jakob Pyszkowski
Version 2.2 Update March 21, 2017 Jakob Pyszkowski
Version 2.3 Update March 29, 2017 Jakob Pyszkowski
Version 2.4 Update May 16, 2017 Jakob Pyszkowski
Version 2.5 Update July 18, 2017 Jakob Pyszkowski
Version 2.6 Update June 20, 2018 Kevin Bielawski
Version 2.7 Update November 7, 2018 Jakob Pyszkowski

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SAFETY
The OP1550 interferometer, part of the Piuma Nanoindenter, is equipped with a class 1M laser. The
laser light is coupled out via a fiber connector on the front panel and has a terminator on the back
panel. Do not view directly into the beam with optical instruments.
The OP1550 is equipped with a 220V/110V plug. Disconnect the instrument before changing the
fuse or before switching from 220V to 110V (or vice versa). Do not open the box, as this might result
in serious injuries.

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INTRODUCTION
This document describes the installation and operation of the Piuma Nanoindenter instrument, as
developed by Optics11. Details of the installation of the Piuma Nanoindenter are provided in
Chapter 1. Chapter 2 describes the preparation of the Piuma Nanoindenter for experiments. How to
perform a measurement is described in detail in Chapter 3. Chapter 4 describes the optimization of
sample preparation and measurement conditions. Chapter 5 provides a description of the Piuma
Nanoindenter’s working principle. In Chapter 6 the Piuma Nanoindentation software suite is
explained in detail. Chapter 7 describes how to operate the OP1550 interferometer, and finally
Chapter 8 provides a FAQ and alternative options for support by a flowchart for troubleshooting.
Figure 1: The Piuma Nanoindenter in action: measuring intact brain tissue.

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1. INSTALLATION
This section describes the installation of the Piuma Nanoindenter by providing an overview of the
Piuma Nanoindenter and its components, the considerations for finding a location to set up the
system, the wiring scheme for connecting the individual components and the actions required to
start up the Piuma Nanoindenter.
1.1 The Piuma Nanoindenter and its components
The Piuma Nanoindenter consists of five components: (1) The Piuma indenter head, (2) the Optics11
OP1550 interferometer, (3) the Piuma controller, (4) the PC running the Piuma Nanoindenter
software suite and (5) the Optics11 optical nanoindentation probes.
Figure 2: Picture of the Piuma Nanoindenter, showing the Piuma indenter head (far left), OP1550 interferometer, Piuma
controller and the PC.

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The Optics11 OP1550 interferometer operates independently of the other components and can be
switched on and off at any time. The Piuma controller box powers the Piuma indenter head and
communicates with the software running on the PC and should not be switched off before exiting
the Piuma nanoindentation software suite. The software will automatically detect and connect to
Piuma components connected to the PC. For the best user experience, it is recommended to first
power on all peripheral components before running the software.
1.2 Connecting components
Together with the Piuma Nanoindenter the following cables are provided:
o2x BNC connector cables
o1x DVI signal cable
o2x Power cable
o1x PC power cable including adapter
o2x USB (B-type) connector cable
o50ΩTerminator
When installing the system, please carefully follow the scheme below (Figure 3).
Figure 3: Schematic drawing of the connections that are required to set-up the Piuma Nano-indenter.
The controller box is connected to the computer using one of the two USB ‘HUB’ connectors on the
back of the Piuma controller, and a free USB slot on the PC (must be a USB3.0 port). The Piuma
indenter head is connected to the controller box using the original DVI cable supplied with the
Piuma system, and a BNC cable connecting the BNC output on the indenters’ backpanel to the

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‘Piezo’input at the back of the controller. The OP1550 readout is connected to controller box using
one BNC and one USB cable. The BNC cable connects the ‘Output’at the front of the OP1550 with
the ‘Input’on the back of controller box and the second USB cable connects the ‘USB’ output on
the back of the OP1550 to the second ‘HUB’ connector on the back of the controller box (Figure 3).
The optional backlight module requires 5V to operate. To power this module, connect the USB
cable to any free USB port on the PC or the controller box.
The optional inverted camera module requires 5V to operate and can be connected to the Piuma
through a free USB HUB port at the rear of the controller box or directly to a free USB port on the
PC.

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2. PREPARING THE SETUP FOR
MEASUREMENT
This section describes the steps required to start and prepare the system for a measurement series.
2.1 Starting the system
Powering up the system
Before starting up the setup for the first time, make sure that all cables are connected in the correct
way and the power switches on the back of the boxes are turned on. Switch on the devices in the
following order:
1. Computer
2. OP1550 interferometer
3. Controller box
The controller box automatically powers the Piuma Nanoindenter head and the LED illumination in
the indenter head will turn on. After initialization, the interferometer will show a live measurement
signal on the LCD screen.
Starting the software
Start the Piuma Nanoindenter program by double-clicking the Piuma software icon on the desktop.
The software is loading all devices while checking the hardware connection status, which should all
be ‘Idle’ (Figure 4). As a safety check, the program asks then to bring up the manual stage to the
upper most position. This prevents a collision of the probe with an obstacle while moving the
sample stage to the home position. To move the manual stage up, turn the grey knobs on the side
of the Piuma backwards until the stage reaches the top position. Next, press ‘OK’ in the software
dialog box to confirm nothing can obstruct the motion of the Piuma stages.
Figure 4: Hardware check.
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