Opentrons OT-2 Manuel utilisateur

OT-2 Liquid Handling Robot

User Manual / Quickstart Guide

Opentrons Labworks Inc.

Edition

Revision OT-2R, June 2021

Product and Manufacturer Description

Product Elements/Overview

Instructions for Unboxing and Use

– Included Box Contents

– Required for Operation

– Machine Setup

– Remove the Transport Fasteners

– Check the Machine

– Pipette Installation

– Pipette Removal

– Power and Connection

– Software Interface

Online Resources

Cleaning and Maintenance

Support

Table of Contents

Post sales service & contacting Opentrons

If you have any questions about the use of the system, abnormal

phenomena, special needs, please contact:

[email protected]. Also visit www.opentrons.com.

OT-2 MANUAL

Product and Manufacturer

Description

Product Elements

PRODUCT DESCRIPTION

The OT-2 is a liquid handling lab robot. It is open-source, highly

customizable, kit and reagent agnostic, and takes up half a lab bench.

Its software and hardware modules are built by scientists for scientists

and don’t require coding knowledge to use.

Completed Certications

CE & FCC, NRTL, & CB

Not Certied/Validated

IVD, GMP & ISO

MANUFACTURER DESCRIPTION

Opentrons Labworks Inc

20 Jay Street, #528

Brooklyn, New York

NY 11201

Serial Number

OT2XXX2021XXXXRXX

OT-2

66CM/26IN

57CM/22.5IN 63CM/25IN

0.1mm resolution

xyz gantry

Polycarbonate

enclosure

Easily removable deck

2 interchangeable

pipette heads

OT-2 MANUAL

Instructions for

Unboxing and Use

Product Elements

DECK OUTLINE

11 deck slots

Adapters allow for existing

hardware to t on deck

Deck slots are compatible with standard SBS

dimensions. Deck also includes a removable trash bin.

Modules snap into place

To get started quickly, visit our online resources and follow the

step by step instructions to help you go from unboxing your

robot to running your rst protocol.

Visit https://support.opentrons.com/ for more information.

INCLUDED BOX CONTENTS / PARTS & ACCESSORIES

◾(1) OT-2

◾(3) Window Covers

◾(2) Side Window Covers

◾(1) Rear Window Cover

◾(1) Power supply (36V/6A)

◾(4) IEC Power cable (Connects the power supply bar to a wall

socket)

◾IEC Cable US

◾IEC Cable UK

◾IEC Cable EU

◾IEC Cable Italy

◾(1) Ethernet Cable

◾(1) Ethernet-to-USB dongle (connects the OT-2 to a laptop)

◾(1) Tool Kit

◾Hand Screwdriver (2.5mm)

◾14mm Wrench

◾Torx Key (T10)

◾Allen key set (1.5mm, 2mm, 2.5mm, 3mm)

◾Super Lube (silicone based lubricant)

◾2pcs M3 hex nut

◾2pcs M4 square nut

OT-2 MANUAL

Box C Included Box Contents

/ Parts & Accessories

◾Pipette Screws

◾4 pieces of M3x6mm socket head screws (2 are spares)

◾Window Screws

◾4x top window screws (M4x12mm low prole socket

screw, M4 thick washer, and rubber O-ring)

◾(1) Trash Bin

◾(1) Calibration Block

(1) OT-2

(4) Window Covers

(2 side window covers, 1

rear window cover)

(1) Power supply (36V/6A)

(4) IEC Power Cable

(Connects power supply bar

to a wall socket)

- IEC Cable US

- IEC Cable UK

- IEC Cable EU

- IEC Cable Italy

ITALY USA

UK EU

(1) Ethernet Cable

(1) Ethernet-to-USB dongle

(Connects the OT-2 to a

laptop)

(1) Torx Key (T10)

(1) Super Lube

(silicone based lubricant)

(4) Pipette Screws

(4 pieces of M3x6mm socket

head screws, 2 are spares)

(1) 14mm Wrench

(1) Allen Key Set

(1.5mm, 2mm,

2.5mm, 3mm)

(1) Hand Screwdriver

(2.5mm)

(2) 2pcs M3 hex nut

(2) 2pcs M4 square nut

OT-2 MANUAL

REQUIRED FOR OPERATION:

◾A computer (self-provided), an open-end wrench (included)

◾Space required by the machine: Space larger than

625mm*570mm*680mm

(4) Window Screws

(4x top window screws (M4x12mm

low prole socket screw, M4 thick

washer, and rubber O-ring)

(1) Calibration Block

(1) Trash Bin

Instructions

MACHINE SETUP

Remove the top foam and window and top covers.

Carefully cut open the plastic VCI bag. Be sure to avoid

cutting any machine components inside.

OT-2 MANUAL

The machine requires two people for lifting out of the

packaging (the machine is over 30kg).

Pull upwards using the black steel frame.

Cut the zip ties supporting the customer kit and place aside.

Place the machine on a secure and stable surface.

Instructions

OT-2 MANUAL

Pull the remaining zip tie ends and remove completely from

the deck.

Remove tape from the calibration switch cover.

Remove the plastic cover completely.

Place the trash bin in place.

Instructions

OT-2 MANUAL

Remove the 3 transport fasteners:

1. Unlock and remove the X-axis locking bracket (red) and 4x

locking screws using the 3mm allen key

2. Unlock and remove the Y-axis locking bracket (red) and 1x

locking screw using the 3mm allen key

3. Unlock and remove the Z-axis locking bracket (red) and 4x

locking screws using the 3mm Allen key. Note that after

removing the rear screws, one can move the z-stage further

left by hand, allowing more room for removing the screw.

Instructions Remove the lm used to protect the polycarbonate sheets

Install the top polycarbonate sheet and fasten the four

corner screws using the 2.5mm hex driver and 4x top

window screws

3 4

OT-2 MANUAL

Check the Machine:

Conrm that 3 brackets have been removed, as powering

on the robot with brackets may cause damage.

Conrm that you are not missing any required cables and/

or parts. If you are missing components, contact Customer

Support.

Visually check the deck and windows, if there are any

signicantly scratched, broken, or bent components,

contact Customer Support.

Visually check the gantry, if it is uneven or slanted, contact

Customer Support.

Move the pipette head in the x and y direction, if it makes

any loud/abnormal noises or requires a large thrust to

move, contact Customer Support.

Clean & Sterilize Robot

Note: When cleaning and maintaining the machine, be sure

to turn o the machine and disconnect the power before

proceeding. Also pay attention to warning labels

Instructions Install window covers

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