NuAire NU 4500 Manuel utilisateur

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Guide

To Components

Air Inlet Filter

Circuit Breaker

On/Off Switch

Front Panel

Fill Port

Drain Valve

Shelf Assembly

Water Pan

Magnetic Door Switch

Outer Door

Inner Glass Door

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2.0

Control Panel and Indicators

HEAT JACKET Indicates Main Heater ON

DOOR AJAR Inner Glass Door Open

LOW WATER Add Water to Jacket

INJECT C02 Indicates Injection of C02

C02TANK

Indicates C02Tank in Use

C02Tank 2 (Option) Indicates C02

Tank

in Use

ALARM Fault Occurrence

[SEL]

Select key allows selection of parameters on display

arrow key allows increase of parameter values

DOWN arrow key allows decrease of parameter values

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SETUP

Mode Switch

Indicates Incubator

is Running or In Setup.

RUN

Mode Indicators

Indicates Present Chamber Temperature

Indicates Present Chamber C02Percent

(Option) Indicates Present Chamber Humidity Percent

RUN

Mode Fault Indicators

Blinking Green LEDNext to Display Indicates Chamber

Temperature Fault.

Blinking Green LEDNext to Display Indicates C02 Percent

Fault.

Blinking Green LEDNext to Display Indicates Humidity Percent

Fault.

All Displays Blinking Indicate Power Interruption.

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3.0

Autoflow Start-up

After your

Autoflow Incubator has been unpacked, positioned and leveled, proceed with the following check list.

1. Check for standard items packed with unit.

(4) Stainless steel shelves

(8)

Stainless steel shelf brackets

(4) Stainless steel shelf bracket supports

(1)Full size water pan with supports

(I)Fill port adapter

(1)Fill port plug

(1)

Gas tube with filter

(1)

feet (2M), fill tube

(1) Electrical Cord

foot (2.5m)

(1)

Operation and Maintenance Manual

(I)Operating Instructions

Install shelf brackets, shelves and water pan. Connect incubatorto an appropriategrounded power source

via the backpanel power cord. Use a dedicated laboratory circuit for the power supply. A SURGE

PROTECTOR IS STRONGLY RECOMMENDED to avoid power-related faults.

Turn mode switch on front panel to setup,

turn

on incubator via the odoffswitch on the backpanel.

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Fill incubator water-jacket with SINGLE distilled or de-ionized water. No purer than

MEGOHM water should

be used. Use enclosed tubing and fill port adapter to fill water-jacket until the low water light on the front panel

turns off. Add an additional

to 2 liters of water and install fill port plug. ABSOLUTELY NO

CHLORINATED ORHALOGEN MATERIALS

ARE

TOBEUSED

THEWATER-JACKET.

4. Attach

C02

gas supply to the

CO2

inlet on the incubator backpanel. Use a TWO-STAGE

REGULATOR

and set

the output pressure to approximately

PSI.

Attach air inlet tubingkilter to the air inlet fitting port on the incubator back panel.

Enter temperature and

C02

percent setpoints (see section 4.0).

Once parameter setpoints are entered, turn mode switch to run and let incubator stabilize for 24 hours. The

C02

percent will indicate "dely" until the incubator reaches within

of setpoint temperature.

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4.0

Setpoint Parameter

Entry

Setpoint values are entered by pressing the

"SEL"

key tothe desired parameter indicator. The value of the

selected parameter will be shown in the display in the form

"XX.X".

To enter a setpoint, perform the following:

Chamber Temperature

Turn Mode Switchto Set Up

Press [SEL]to Indicate Green LED Next to Chamber Temperature Display

Press

to Indicate Desired Temperature

Turn Mode Switch Back to Run

CO?

Percent

Turn Mode Switch to SetUp

Press

[SEL]

to Indicate Green LEDNext to C02Percent Display

Press

to Indicate Desired C02Percent

Turn Mode Switch Back to Run

Humidity

(Optional)

Turn Mode Switch to Set Up

Press [SEL]to Indicate Green LEDNext to Humidity Percent Display

Press

or to Indicate Desired Humidity Percent

Turn Mode Switch Back to Run

NOTE: If an entry error is made, re-enter data.

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5.0

Chamber Temperature Calibration

The US Autoflow's TEMPERATURE CALIBRATION MUSTBE PERFORMED

WITHIN

1°C

THE

PLANNED OPERATING TEMPERATURE. Normally,

37.0°C

is the most common setpoint. With the incubator

operating at its setpoint, place a mercury glass thermometer in a glass beaker filled with water or through an inverted

Styrofoam cup resting on a shelf in the middle ofthe chamber. Do not place the glass beaker on the bonom of the

incubator chamber. Placing the thermometer in the middle of the chamber will givethe most accurate results for

calibration. Allow the thermometer

hours to stabilize.

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When the temperature is stabilized atthe operating temperature, perform the following calibration procedure.

Turn Mode Switch to Run

Press

[SEL]

to indicate green

LED

next totemperature display

Press and hold one

key for four seconds, display blinks

Press

or key to indicate sametemperature asthermometer

Press

[SEL]

key to complete calibration

The chamber temperature calibration is complete. Let unit stabilize for

hours. If the chamber temperature (actual

thermometer) still does not match the display, perform the above procedure again. In some cases it might be necessary to

calibrate several times to achieve a stable condition due to ambient conditions of temperature and humidity within the

laboratory.

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6.0

Door Temperature Calibration

The US Autoflow's inner glass door temperature calibration is best accomplished by running the incubator

hours with the water pan in place and perform the following calibration sequence if required.

Open the incubator door and look for general condensation. Somecondensation on the glass door is desirable as an

indication of adequate humidity in the chamber. Typically one to two inches of condensation in the corners of the glass

door indicates a properly calibrated door heater. However, if calibration is required, simply perfom the procedureas

stated below. The door heater operates as a percentage

ONlOFF (0

is off,

100

is full

ON).

Typically,

percent is

the most effective setpoint range.

Turn Mode Switch to Set Up.

Press [SEL]to indicate green

LED

next to temperature display.

Press both +or

keys simultaneously, display "dor"1door heat

(XX).

Press

key to desired door heat

on.

Press [SEL]to complete the calibration.

Turn Mode Switch back to Run.

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