Neogen ANSR Manuel utilisateur

1. Introduction ..................................... 2

2. Warnings and limitations.................. 2

3. Materials provided ........................... 2

4. How it works.................................... 2

5. Using ANSR with a computer............ 3

6. ANSR stand-alone operation .......... 10

7. Cleaning and maintenance............. 12

8. Troubleshooting ............................. 12

9. Limited warranty............................ 12

10. Terms and conditions..................... 12

11. ANSR reader speciﬁcations............ 13

12. Neogen company proﬁle ................ 14

USER

MANUAL

USER

MANUAL

ANSR reader and software

2User Manual •Neogen ANSR reader and software

10. Keep connected cables clear of work areas. The main socket

outlet is intended to be used with the reader’s external power

pack and should be readily accessible. It is recommended that

the user unplug the reader when not in use.

11. If intended for connection to external equipment, ensure the

interfaces of such equipment are separated from the main

socket by double or reinforced insulation and present no risk of

electrical shock.

3. Materials provided

•Neogen ANSR reader

•Computer with pre-installed software (Windows 7/10)

•Case

•4 power cord plug adapters

•Ethernet cable (if purchased with computer)

•USB cable

•Power cord

4. How it works

The ANSR reader uses two channels of light, referred to as Control

and Test, to analyze samples. Fluorescence is generated by peri-

odically exposing the reagents in the tubes to the Control and Test

wavelengths. The resulting ﬂuorescence is captured by photodiodes,

where it is converted to millivolts as a function of time. Mathematical

algorithms are applied through the software to signal the detection of

target pathogens in the sample.

1. Introduction

This manual describes setup, conﬁguration and operation of a reader

and software for use with the Neogen ANSR®pathogen detection

system.

The incubation function of the reader has been set to maintain a con-

stant temperature of 56 ± 1°C to meet the speciﬁc requirements of

the Neogen ANSR pathogen detection system.

This reader has been designed to operate in one of two methods:

1. With a dedicated personal computer and ANSR software package

to track, interpret and analyze assay results.

2. As a stand-alone unit that displays assay results on the reader’s

screen.

The ﬂexibility of the Neogen ANSR pathogen detection system allows

users to evaluate up to 48 samples an hour depending on an indi-

vidual laboratory’s workﬂow needs.

For more information on the use of the reader, or the Neogen ANSR

pathogen detection system, please contact NeogenTechnical Services

or a Neogen sales representative at 800/234-5333 or 517/372-9200.

2. Warnings and limitations

1. Always operate the instrument on a dry, level surface out of

direct sunlight.

2. Never move the instrument while a test is in progress.

3. To avoid damaging the reader, never place objects on top of it.

4. The heater block in the reader operates at up to 56˚C and may

cause contact burns or damage to materials in contact with

these areas.

5. This instrument is designed only to operate with the provided

power supply plug pack. This module forms part of the system.

Do not operate the system with a different power supply module.

The correct power supply is required to maintain the safety and

electromagnetic compatibility of the system.

6. To avoid electrical shock, do not operate the instrument or the

power supply plug pack if it has been opened, damaged or

exposed to moisture, condensation or rain. The external power

supply plug pack is sealed with no user serviceable parts. Do not

operate this module with any damaged or exposed parts.

7. Do not open or attempt to repair the reader or other accessories.

This instrument does not contain serviceable parts and should

be returned to the supplier for repair. Attempting to service the

instrument also will void the warranty.

8. The real-time clock coin battery included in the equipment is not

a user replaceable item.

9. Only use the instrument for its intended purpose and in

accordance with this manual and warnings. Protection provided

by the equipment may be impaired if the equipment is operated

in a manner contradictory to the instructions and warnings. This

instrument (including power supply) is designed to operate

within the manufacturer’s speciﬁcations. Do not exceed the

manufacturer’s speciﬁcations when in use.

Questions? Call Neogen at 800/234-5333 or 517/372-9200

Turning the reader on and off

To turn the reader on, press the rectangular power button located on

the lower left corner of the reader’s front panel for 2–3 seconds. The

reader will take about 2 minutes to boot.

To turn the reader off, press the power button for 2–3 seconds.

5. Using the ANSR reader with a computer

(remote mode)

5.1 Set up

Setting up the reader depends on the desired usage: with a computer

or stand-alone. If using a computer, ensure the reader is connected

to the laptop before turning on the reader. Once the reader is turned

on, it will heat to its resting temperature. NOTE: The reader’s default

setting is remote mode. To switch a reader previously used for stand-

alone mode to remote mode, see Section 6. The reader is in stand-

alone mode if the touch screen displays three icons upon start up: a

test tube, a set of gears and a magnifying glass.

Remote mode (connected to a computer)

This mode allows the reader to be used with a computer and the

Neogen ANSR software. Results will be displayed on the computer

screen. NOTE: Prior to starting the reader, ensure connected computer

is on and the ANSR software is running.

1. Plug the reader into a power source, using one of the provided

outlet adapters if necessary. NOTE: If the computer is to be con-

nected to a network, do so prior to connecting the reader to the

computer.

2. Using either the provided USB cable or the Ethernet cable, con-

nect the reader to the computer by plugging the cable into the

appropriate port on the back of the reader. Connect the other end

of the cable to the provided laptop. NOTE: If using an Ethernet

cord to connect the reader to the computer, the computer will

not be able to connect to a network via Ethernet.

3. Press the rectangular power button located in the lower left cor-

ner on the reader’s front panel for 2–3 seconds. The reader will

take about 2 minutes to boot.

ANSR reader with case, power cord, USB cable,

Ethernet cable, and alternate plug adapters

4User Manual •Neogen ANSR reader and software

5.2 Installing the software

The ANSR reader software comes pre-installed on the computer pro-

vided in the start up system.

5.3 Using the software

5.3.1 Launching the software

1. Open the reader program by double clicking on the

T16 ISO desktop icon.

2. Before running tests, the computer must be connected to the

reader. To do this, click the Connect icon in the upper right

corner of the software window. Select either USB or Network-

ing, depending on the cable used to connect the reader to the

computer. Select the appropriate choice and hit Connect. The

circle in the upper right corner will turn green and indicate the

reader is connected. NOTE: Users also may use the Locate but-

ton, which is in the Connect menu to ensure the reader is con-

nected. Clicking Locate will cause the reader to emit a beep.

This feature only is available when the reader is connected via a

networking cable.

5.3.2 Software utilities

Open: To open a previously saved ﬁle, click Open and select the ap-

propriate .json ﬁle. NOTE: The reader does not need to be connected

to the computer in order to use the software.

Print: Allows users to print a summary report and/or tube graphs.

Export: Allows users to export all ﬁles associated with the test or only

desired ﬁles. Click Export and select which ﬁles to export by check-

ing or unchecking boxes located next to the options. Click Export

once all selections are made. See example below.

Save as: Allows the user to select the location where they would like

the data saved.

Support: This button displays a copy of the user manual.

Instrument: This button displays software and ﬁrmware version in-

formation.

5.4 Running a test (with computer)

1. Once the reader is set up and connected to the software, tests

can be run. IMPORTANT: Do not open the reader’s lid when the

test is running.

2. To begin a test, click Run and select which test the reader will

perform (e.g., ANSR Salmonella) from the drop-down menu.

3. The screen will read, “Please wait. Setting temperature.” It

also will display the target temperature of 56°C and the instru-

ment’s current temperature. During this time, users may enter a

user name, lot number and sample identiﬁcation numbers. Us-

ers may manually type in sample identiﬁcation numbers or may

use the Autoﬁll option, which is activated by entering a sample

ID in the ﬁrst ﬁeld and clicking the drop-down arrow. Autoﬁll

then will ﬁll all 16 ID ﬁelds with sequential IDs. Numbers also

can be added by copying and pasting ﬁelds from Microsoft Excel.

NOTE: If no sample ID is entered, the result will not be displayed.

Also note that user name, lot number and sample IDs cannot be

edited after the test run has started. The sample ID must have a

number at the end to use Autoﬁll.

4. Once the instrument is ready, the window will display, “Instru-

ment ready”. After placing the samples in the reader, press the

Start workﬂow button near the bottom of the screen to begin

the test. NOTE: For instructions on preparing samples and re-

agents, and on loading them into the reader, see the instructions

that came with the

ANSR test kit. Wait to

add samples until the

reader reaches 56°C.

Ensure the permanent

caps are placed onto

the tubes to avoid

contamination.

5. During the test, the

user can select from

a range of real-time

graphs on the left side

of the program win-

dow. Individual tubes

Questions? Call Neogen at 800/234-5333 or 517/372-9200

can be selected along with a total view, which will show the for-

mation of the curve that denotes a negative or a positive result.

6. Once the assay is complete, the software will display an overall

graphical view of the results. See example above.

7. Click Results on the left side of the window to view a graphic

depicting whether the tubes were positive (red), negative (green)

or invalid (yellow). On the left side of the window, users have the

option to click on individual tube tabs for graphs related only to

one sample, all of the graphs together, or temperature data. To

view the Summary report, which contains a summary of the

test results, click Print and check the box for Summary report.

Users also can print the Tube graphs from the Print menu. Once

the desired selections are made, click Print preview to review

the selections, and click Print.

8. The data can be saved to the computer or exported. To export

data, click the Export icon in the toolbar. Select which data ﬁles

to export (e.g., the data ﬁle, a .csv ﬁle for use in Microsoft Excel,

or a graph). NOTE: The data ﬁle is the most important and con-

tains results and traceability information. It is saved as a .json

ﬁle and only can be opened using the ANSR software. The data

automatically is saved in the Records folder on the computer’s

C: drive (Program ﬁles(x86)/Axxin/AxxinT16ISODesktop/

TestResults/Records). The default ﬁle name includes the in-

strument ID, date and time. The ﬁle extension is .json. This ﬁle

type can only be opened using the ANSR software. The user can

use the Save As function to save the ﬁle with a speciﬁc name

and in a user-speciﬁed location.

9. To begin a new test, click New in the toolbar. The software will

autoﬁll the user name and lot number. These may be edited.

NOTE: Ensure data is saved before clicking New.

10. To shut down the reader, click Disconnect in the upper right

corner of the software window. Once the reader is disconnected

from the software, the computer and reader may be shut down.

Running high throughput samples – Two step lysis

(ANSR for Salmonella and ANSR for Listeria)

NOTE: This procedure begins after the 37°C incubation step. See the

ANSR for Salmonella or ANSR for Listeria kit insert for further instruc-

tions.

The ANSR reader can process 32 samples (Listeria) or 48 samples

(Salmonella) an hour by adhering to the following procedure:

1. Follow the procedures for preparing and enriching the samples

in the ANSR kit insert. Ensure the reader is on and heated

to 56°C.

2. Place up to four rows of lysis tubes containing samples in the

80°C heater block for 20 minutes per the kit’s instructions.

3. Transfer the lysed samples from the ﬁrst row of tubes to reac-

tion tubes 1–8 (from the left) in the reader. Transfer the lysed

samples from the second row of tubes to reaction tubes 9–16

(from middle) in the reader. Follow pipetting, mixing and capping

procedures as listed in the ANSR kit insert.

4. Close the reader’s lid and run the test by pressing Start on the

software screen.

5. Once the 10 or 18 minute assay is complete, save the results to a

desired location. Discard the used reaction and lysis tubes once

results have been obtained. NOTE: After each run of 16 samples,

select New for the next set of 16. The remaining lysis tubes

containing samples may remain in the heater block for up to

60 minutes.

6. Complete the assay with the next two rows of lysis tubes con-

taining samples.

7. Repeat steps 3–6 until all samples have been run.

6User Manual •Neogen ANSR reader and software

5.5 Open Remote List NEW

Open Remote List allows a user to access and download test ﬁles

saved on a remote server.

Users can navigate SFTP or FTP servers, and download relevant re-

sults for review. The T8 desktop software supports viewing (and list-

ing) of T8 results only (.csv ﬁles).

5.5.1 Add and Remove ‘Open Remote List’ Proﬁles

To set up a remote connection, click on the Open Remote List button.

From the drop-down list, select New Connection.

When prompted, ﬁll in the remote server’s details in the Add Connec-

tion window.

The following ﬁelds must be ﬁlled in:

• Address – IP address or domain name of the remote server. eg:

118.168.4.75 or sftp.example.com

• Type – The type of server the user is connecting to. This can be

either SFTP or FTP.

• Port – The port number through which the server can be reached.

•

Destination Directory – The user can explicitly specify which

subdirectory (on the remote server) should be viewed. If this ﬁeld

is left blank, the root directory of the server will be displayed.

• User Name/ Password – The user’s login credentials for the

remote server.

It’s useful to click on the Test Connection button to conﬁrm the en-

tered details are valid.An error icon will be displayed if the connection

was unsuccessful, along with further details for troubleshooting.

Hovering over the error icon will provide helpful information for trou-

bleshooting

A few common connection errors are:

• Incorrect IP address

• Incorrect server type – the desktop software supports connect-

ing to SFTP/FTP servers

• Incorrect or invalid user login credentials – The user must have

access to the server for results to be listed.

If all ﬁelds are valid and correct, Test Connection will indicate that a

connection could be successfully made.

Test Connection was successful. The Open Remote List proﬁle can

now be added.

The user can now add the proﬁle, by clicking the Add button.

Once a proﬁle has been created, the user can add other proﬁles too,

to browse other desktop servers.

A list of other valid Open Remote List proﬁles. Each will pull results

from a different server.

To delete a proﬁle, click the red ‘X’ icon as shown below:

5.5.2 Browsing, Viewing and Downloading Results

Once a valid connection has been established, the user can now

browse results on the remote server.

Questions? Call Neogen at 800/234-5333 or 517/372-9200

A collection of test result ﬁles on the remote server.

The user can navigate up a folder by clicking “Open” on the Parent

Folder. Similarly, clicking “Open” on a subfolder will display that sub-

folder’s contents.

A user can also search for test results using the search ﬁeld.

The search ﬁeld will look for matching ﬁlenames, and will return a list

of partial matches as well.

Once a relevant result has been located, click on the Load button:

View a result by clicking Load.

The result is loaded into the desktop view:

Viewing a result remotely does not automatically download it. To do

this, click on the “Export” button, and provide a local location for the

ﬁle to be saved to.

5.6 Sync Page NEW

The T16-ISO desktop now supports synchronizing test results saved

on the laptop to a remote server. This is known as the “Sync” func-

tion. This Sync is unidirectional; results can only be transmitted to

the server, and not retrieved. Up to two remote Sync proﬁles can be

conﬁgured for use.

A sync proﬁle will look for test records to transfer to the server. It’s

important to note that only results that reside in the Records folder

will be synchronized. By default the records folder is created in C:\

Program Files (x86)\Axxin\Axxin 16 ISO Desktop\TestResults\Records

In addition, the software can also be conﬁgured to act as a server,

and receive test results when transmitted by another desktop sync

proﬁle. This is convenient when several instruments are in use, and

it’s necessary to store a copy of all results in one centralized location.

5.6.1 Add an Outgoing Connection

Adding an ‘outgoing connection’ can be thought of as creating an

automatic backup. When an outgoing connection is conﬁgured, any

new results will be transmitted to the designated server.

To create a sync proﬁle, click the ‘Add Outgoing Connection’ button.

When prompted, enter the required server details:

The following ﬁelds need to be set:

• Address –The address of the remote server.(E.g.: 192.168.10.10

or server.example.com)

• Type – Selected between SFTP and FTP

• Port – The server access port number

• Destination Directory – The location where ﬁles will be stored

on the server. (E.g.: MyT16Results will store the results in server.

example.com/MyT16Results)

• User Name/ Password – Authentication credentials for the

server

• Periodic Sync – If enabled, the software will transfer any un-

synced results every 10 minutes to the server. If disabled, the

user must manually initiate the Sync function.

8User Manual •Neogen ANSR reader and software

Click Test Connection to conﬁrm that with a valid username and pass-

word, a connection can be made to the server. If successful, a green

‘success’ icon will be displayed:

If unsuccessful, the software will indicate which ﬁeld was invalid.

In the above screenshot, the login credentials were incorrect.

Hovering over the red exclamation icon will provide more information

about the error.

5.6.2 Sync Operation (Outgoing Connections)

Two outgoing connection proﬁles can be conﬁgured for use. It’s im-

portant to note that while a test is running, no Sync operations will

be performed.

The \TestResults folder is the ‘root’ folder which the Sync function

will search through to ﬁnd ﬁles. Additionally, the Sync function will

search 3 levels deep through subfolders for other ﬁles to transmit. It’s

important to note that test results are saved in \TestResults\Records

by default.

As an example, any ﬁles that have been saved in \TestResults\Re-

cords\SubFolder will be transmitted, however, data in \TestResults\

Records\SubFolder\AnotherFolder will be ignored.

NOTE: Sync is restricted to transmitting only .csv ﬁles, the main ﬁle

format exported/saved by the T8 desktop software. Other ﬁles in the

\Records folder (if any) are not transferred.

NOTE: Result ﬁles larger than 5Mb will not be transferred. During

normal operation, it is unlikely that a result ﬁle larger than 5Mb will

be generated.

Clicking on the ‘Sync Now’ button will force the desktop software to

connect and transmit data to the server.

Clicking on the ‘More’ button will display additional proﬁle options:

• Remove – Will remove the sync proﬁle.

• Edit – This will allow the user to edit the sync parameters for the

proﬁle, as in Figure 61.

• Test Connection – Test the current connection parameters. If

the connection was successful, a green tick icon will be dis-

played next to the test connection button. If unsuccessful, a red

error icon is displayed.

• Enable/Disable Periodic Sync – Toggle between Periodic Sync

being enabled or disabled. Once enabled, the Sync process will

begin immediately to transmit any unsent results.

While ﬁles are being transmitted, the sync proﬁle displays the text

‘Running’ and begins counting down the number of ﬁles remaining

to be pushed to the server. The user cannot trigger a manual sync

or modify the contents of the sync proﬁle while a Sync is in progress.

Once all ﬁles have been successfully pushed to the server, the User

Interface will display ‘Synchronized’. If periodic send is enabled, the

time the last transmission was successful will be displayed along

with the time the next Periodic Sync will happen.

5.6.3 Add Incoming Connection (Server)

The software can be conﬁgured as a server, to receive data transmit-

ted by a sync proﬁle (outgoing connection) from another instance of

desktop software on a separate computer.

Click Add Incoming Connection:

The following ﬁelds need to be ﬁlled out by the user:

• File Storage Path – The location where received results will be

saved.

Questions? Call Neogen at 800/234-5333 or 517/372-9200

5.6.4 Sync Server Operation (Incoming Connection)

Once a server is added, the desktop software is now ready to receive

test results.

Only 1 Sync server (Incoming Connection) conﬁguration can be set up

and active at any time. It’s important to note that the sync server will

only run as long as the desktop software is open; if it is closed, any

proﬁles which are conﬁgured to transfer results to it, will be unable

to do so.

A server is set up and running on these IP addresses.

The following pieces of information are displayed to the user:

• The local IP address(es) on which the server can be connected

to. (Note: if attempting to connect from a computer that is not on

a local network, use your global IP address instead.)

• The Connection Type – the server type is SFTP

• Auto-start – Whether the server will automatically start once

the desktop software starts

• Port – the port on which data can be transmitted

• Storage Location – The folder in which received data will be

stored.

• Server state – Online or Ofﬂine

• Current Connections – The number of users currently connect-

ed to the server

• Files Received – The total number of ﬁles received for the dura-

tion of the server session (i.e.: from the last time the desktop

software was opened).

• Last File Received – The ﬁlename of the last ﬁle transmitted.

• Start Server on Instrument Startup – If this is checked, the

server will be online and ready to receive ﬁles, as soon as the

desktop software is powered up.

• Connection Port – Specify the port number over which results

can be received.

• User management – At least 1 user needs to be created before

a server can be set up. (see section below)

5.6.3.1 Conﬁguring Users

To create a user, enter a user name between 5 and 20 characters.

Enter a password for this user. The password must:

• be between 6 and 20 characters

• contain at least 1 capital letter

• contain at least 1 number

• contain at least 1 non-alphanumeric character (eg: #, $, @)

• not match the username.

Click on “Show Passwords” at the bottom of the panel to simplify

user creation.

To delete a user, click on the red “X” next to their entry.

10 User Manual •Neogen ANSR reader and software

6. ANSR stand-alone operation

6.1 Set up

To operate the reader without the use of a computer or a network

connection, plug the reader into an appropriate power outlet. Turn

the reader on by pressing the rectangular power button located on

the lower left corner of the reader’s front panel for 2–3 seconds.

The reader will take about 2 minutes to boot. Once ready to use, the

screen will display three icons: a test tube (Run test), a magnifying

glass (Search), and gears (Settings).The reader is now ready for use.

6.2 Printer set up

1. Touch the gears (Settings) icon on the Home screen.

2. Touch the down arrow followed by the Printer Set up icon.

3. Using the touch screen key pad, enter the IP address of the de-

sired printer and press the green check mark to conﬁrm.

6.3 Remote mode

To switch from stand-alone operation to operation with a computer

(remote mode), users must ensure remote mode is activated prior to

attaching the reader to a computer. For instructions on how to set up

the reader for use in remote mode, see Section 5.

1. Touch the magnifying glass (Settings) icon on the Home screen.

2. Touch Remote mode. Touch the box in the lower right corner

next to Bootup into remote mode. The next time the reader is

turned on, it will be in remote mode.

6.4 Settings

About this device

This option provides information about the instrument name, ID, ap-

plication version, IP address, MAC address, OS version, kernel version

and light channel information.

1. To access About this device, touch the gears (Settings) icon

on the Home screen.

2. Select About this device. The information will be displayed.

Help info

1. To access Help info, touch the gears (Settings) icon on the

Home screen.

2. Select Help info. The information will be displayed.

Audio

This option allows the user to change the volume of the reader’s tones.

1. To access Audio settings, touch the gears (Settings) icon on

the Home screen.

2. Touch the Audio icon.

3. Use the up and down arrows to adjust the reader’s tones for

the touch screen and for alerts (0–low, 5–high). Press Test to

hear the sound. To conﬁrm the selection, press the green check

mark.

Brightness

This option allows the user to adjust the brightness of the touch screen.

1. To access Brightness settings, touch the gears (Settings) icon

on the Home screen.

2. Touch the Brightness icon.

3. Using the white up and down arrows, adjust the brightness of

the screen to the desired level (30–low, 100–high). The screen

brightness will change as the user touches the arrows. Touch

the green check mark to conﬁrm the selection.

6.5 Administrator settings

Administrator settings are password protected. For the password,

please contact a Neogen Technical Services representative.

Import and export

1. Touch the gears (Settings) icon on the Home screen.

2. Touch the Administrator settings icon. Enter the password and

touch the green check mark to conﬁrm.

3. Select Import and export functions. Plug in a USB drive to the

port located on the back of the reader.

4. Touch the Export test results icon. This will export results as a

.json ﬁle that can only be read using the ANSR software.

5. The reader will ask if a copy of the exported results should re-

main on the reader or be deleted. Make the appropriate choice

and touch the green check mark to conﬁrm.

Export data log

This option exports a folder named with .json ﬁles (which only can be

opened using ANSR software) and .csv ﬁles, which can be opened in

Microsoft Excel.

1. Touch the gears (Settings) icon on the Home screen.

2. Touch the Administrator settings icon. Enter the password

and press the green check mark to conﬁrm.

3. Select Import and export functions. Plug in a USB drive to the

port located on the back of the reader.

4. Select the Export data log icon. The reader will export a folder

containing a .json ﬁle and a .csv ﬁle.

Import test types

To import a new test type, please contact Neogen Technical Services.

Time and date

This setting allows the user to adjust the date and time on the reader.

1. Touch the gears (Settings) icon on the Home screen.

2. Touch the Administrator settings icon. Enter the password

and press the green check mark to conﬁrm.

3. Select Time and date.

4. Use the touch screen to select the item to be changed (e.g., a.m.

or p.m., the month, the hour) and use the white up and down

arrows to scroll through the options until the desired informa-

tion is reached.

5. Select the green checkmark to save the time and date settings.

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