NanoEnTek EVE Manuel utilisateur
User
Manual
EVE
™
Automatic cell counter
2
All the materials in this user guide are protected by Korean and
international copyright laws. They cannot be reproduced, translated,
published or distributed without the permission of the copyright owner.
EVE™ User manual
Website : www.nanoentek.com
Manufactured by
NanoEnTek Inc. (HQ)
12F, 5, Digital-ro 26-gil, Guro-gu, Seoul, 152-740, Korea
Tel : +82-2-6220-7940
Fax: +82-2-6220-7721
The information in this manual is described as accurately as possible.
Firmware and software changes and updates may change without prior
consent or notification.
Copyright © 2013 by NanoEnTek Inc.
All rights reserved. Published in Korea.
Documentation : NESMU-EVE-001E
Revision history : V.0.0 Date : MAR 2013
: V.0.5 Date : MAY 2013
: V.0.8 Date : OCT 2013
: V.1.5 Date : MAY 2014
EVETM, Automatic Cell Counter ©2013 NanoEnTek Inc. 3
Table of contents
Product contents
Product overview
Description
Installation
Operation
Settings
Cell mode parameters
Date and time setup
Recommend actions
Cell counting
Transferring data to a computer
Using the beads mode for counting beads
Calibrating
PC software
Installation
Open the data
Cell gating
Counting
Spread sheet
Prepare report
Cleaning and maintenance
Troubleshooting
Error codes
Warranty
Safety precautions
Product specifications
Ordering information
Technical support
4
5-6
7-8
9
10-25
10
10-12
13
14
15-20
21
22-23
24-25
26-33
26-27
28-29
30
31
32
33
34
35-36
37
38
39-41
42
43
44
4
Product contents
EVE™ is shipped with the following components.
Please check that all items listed below were shipped, receiving the instrument.
If any items are missing or damaged, contact your local distributor or e-mail
Automatic cell counter
1 EA
USB drive
4 GB
1 EA
User manual
1 EA
Power cord with 4 adaptor
cords ( 4 pcs/set, for U.S./Canada/
Taiwan/Japan, Europe or UK)
1 SET
Cell counting slides
(with 1.5 mL of Trypan blue (0.4%))
1 BOX (50 slides/box)
Quick manual
1 EA
Cat. No. EVE-MC
Cat. No.
EVS-050
EVETM, Automatic Cell Counter ©2013 NanoEnTek Inc. 5
Product overview
The EVE™ Automatic cell counter uses state-of-the-art optics and image
analysis to automatic cell counting. The EVE™ is a benchtop counter designed to
measure cell count and viability (live, dead, and total cells) accurately and precisely,
using the standard trypan blue technique.
Using the same amount of sample that you currently use with the hemocytometer, the
EVE™ takes less than 20 seconds per sample for a typical cell count and is compatible
with a wide variety of eukaryotic cells and provides information on cell size.
The EVE™ offers an intuitive user interface, and provides the option to save and print
cell count data using the EVE™ Software (download from www.nanoentek.com )and
USB drive supplied with the instrument or available separately.
The EVE™ is supplied with disposable EVE™ Cell counting slides that contain two
enclosed chambers to hold the sample to allow you to measure two different samples or
perform replicates of the same sample. The cell counting occurs in the central location
of the counting chamber and the volume counted is 0.4 µL, the same as counting four
(1 mm × 1 mm) squares in a standard hemocytometer.
6
Product overview
Features and benefits
—
User-friendly, benchtop design for simple, fast, automated cell count and
viability measurements within 20 seconds.
—
Provides data on cell size and is compatible with a wide variety of eukaryotic cells
without the need for any special changes between large or small sizes.
—
Measures cell concentrations ranging from 1 × 104to 1 × 107cells/mL and
cells with sizes ranging from 5 µm to 60 µm.
—
Provide the clumping cell counting function to get more accurate results
(using EVETM PC software).
—
Uses disposable cell counting slides that eliminate washing steps and
cross contamination between samples.
—
Saves and print cell count data including images using the EVE™ software and
EVE™ USB drive.
—
Presents comprehensive data with graphical reports and as a .CSV
(comma separated value) file for sample comparisons.
EVETM, Automatic Cell Counter ©2013 NanoEnTek Inc. 7
Description
Front view The power button is used to turn the instrument
on and off. The red status light indicates that
the instrument is off; the green status light
indicates that the instrument is on.
Located in the front of the instrument contains
buttons for all the functions needed and
displays data from the cell count.
The slide port is used to insert the EVE™
Cell counting slide containing the sample with
trypan blue stain into the counter for analysis.
The USB port allows you to transfer and save
the cell count data and image to your computer
for record keeping and printing purposes. The
USB drive supplied with the instrument or any
other standard USB drive is inserted into the
USB port for data transfer.
Power button
LCD touch screen
Slide port
USB port
[Front view]
Power button
Slide port
USB port
LCD
touch screen
8
Description
Rear and
side view
Connect the counter to an electrical outlet
using the supplied power cord and the
appropriate plug, based on the electrical outlet
configuration in your country.
The image adjustment (focus) knob is used
to adjust the image quality to obtain better
contrast between live (bright centers) and dead
(dark blue centers) cells. This is important
to obtain accurate cell counts and viability
measurements.
The focus lock knob is used to lock the image
adjustment (focus) knob once the image is
optimized. There is no need to use the focus
lock knob , but is available for convenience,
if you are measuring multiple samples of the
same cell type.
Power inlet
Image adjustment
(focus) knob
Focus lock knob
[Rear view] [Side view]
Power inlet Focus lock Focus knob
EVETM, Automatic Cell Counter ©2013 NanoEnTek Inc. 9
1. After unpacking the instrument, place the instrument on a
flat, level, dry surface.
3. Plug the power cord into the electrical outlet. Be sure to use
only the power cord supplied with your instrument. Powering the
instrument with an unapproved power cord may damage the
instrument.
5. When the instrument is turned on, the Start Up screen is
displayed. Here you can proceed immediately to cell counting,
set up the instrument for cell or bead count, or adjust the screen
brightness.
Installation
Installing
EVE™
Automatic
cell counter
2. Plug one end of the supplied
power cord into the EVE™. To the
other end attach the appropriate plug
adaptor, based on the electrical outlet
configuration in your country.
4. When you are ready to use, start the EVE™ by pressing the
Power button.
10
Operation
Settings
Cell mode
parameters
1. Press Settings from the Start-Up screen to display Settings.
The settings menu allows you to set up the following:
· Count mode to select Counting type and Auto focusing options. The Counting
type is to operate an instrument for cell counting or beads counting. The Auto
focusing is to operate an instrument for AF on mode or AF off mode.
· Count mode to operate the instrument for cell counting (choose Cells
or bead counting (choose Beads)
· Parameters (see below and next page for details)
· Calibration to calibrate the instrument (page 24)
· Update to install new firmware versions as they become available
· Date and Time to set up date and time (page 13)
· Use the scroll buttons to adjust the screen brightness.
1. Press Parameters from the settings screen to display Cell mode
parameters screen.
The Parameters function allows you to change the image analysis algorithm
for specific or mixed cell types, and the specific parameters must be
determined empirically.
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1
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