MVE Fusion Series Manuel utilisateur

MVE Fusion Series

MVE FUSION Quick Reference Guide
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Quick Reference Guide
Table of Contents
NOTE: MVE Fusion cryogen freezer should be installed by Chart Personal or an
authorized MVE Distributor per the MVE Fusion Technical Manual, PN 20994124.
Product Identification
Safety…………………………………………………………………………………………………………………………………………3
Display / Control Panel……………………………………………………………………………………………………………….7
Back Panel / Electrical / Physical Connections ……………………………………………………………………………8
Dewar Plumbing Connections…………………………………………………………………………………………………….9
Setup/Filling Procedure ……………..……………………………………………………………………………………………..9
Calibration of Temperature Probe.…………………………………………………………………………………………….14
Alarms and Definitions……………………………………………………………………………………………………………….15
Contact Information…………………………………………………………………………………………………………………..15

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Safety
READ BEFORE OPERATING THIS EQUIPMENT
Liquid nitrogen (LN2) is used in MVE Fusion Freezers as a refrigerant. Understanding and
following certain safety precautions is extremely important when handling LN2 and cryogenic
containers (Dewars).
Liquid Nitrogen Properties
Nitrogen is a colorless, odorless, tasteless gas. Gaseous nitrogen makes up about 78% of the
Earth’s atmosphere by volume. Once collected and isolated, nitrogen can be liquefied.
Boiling Point @ 1 atm -195.8°C , -320.3°F , 77.4 K
Thermal Conductivity (Gas) 25.83 mW/(m·K)
Heat of Vaporization (Liquid) 198.38 kJ/kg
Density @ 1 atm (Liquid)
1.782 lbs/L , 807.4 g/L , 808.6 kg/m3
Liquid Nitrogen Safety
Transferring LN2 and operating the MVE Fusion should be done in accordance with the
manufacturer / supplier instructions. It is important that all safety precautions recommended by
the manufacturer be followed.
Nitrogen vapor is a potential asphyxiant as it displaces Oxygen (O2)
in confined spaces. Rapid suffocation can occur without warning in
an Oxygen-deficient atmosphere (less than 19.5% O2). Chart
Cryogenic Freezers must be installed and operated in well-ventilated
areas.
DO NOT vent container in confined spaces.
DO NOT enter confined spaces where excess nitrogen gas may be
present.

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If exposure has occurred move to ventilated area or fresh air. If
breathing is difficult, supplement oxygen may be required. If not
breathing, give artificial respiration. SEEK IMMEDIATE MEDICAL
ATTENTION.
Contact with liquid nitrogen or uninsulated equipment containing
nitrogen can result in cold contact burns or tissue damage.
Nitrogen vapor can cause damage to skin or eyes.
In case of frostbite, warm area with warm water not exceeding 105°F
(40°C) and SEEK IMMEDIATE MEDICAL ATTENTION.
Never place LN2 in a sealed container without a pressure relief
device. The expansion ratio of liquid nitrogen to gaseous nitrogen
is 1 to 700 (1 cubic foot of liquid nitrogen becomes 700 cubic feet of
gaseous nitrogen when evaporated).
The two most important safety aspects to consider when handling LN2 are adequate ventilation
and eye and skin protection. Although nitrogen gas is non-toxic, it is dangerous in that the gas
will displace oxygen in a normal breathing atmosphere. Liquid products are of even greater threat
since a small amount of liquid evaporates into a large amount of gas. Therefore, it is imperative
that cryogenic supply and storage Dewars be stored and operated in well-ventilated areas.
Persons transferring LN2 should make every effort to protect the eyes and skin from accidental
contact with liquid or cold vapor. Chart MVE recommends the following protective clothing and
accessories when transferring LN2 or handling hoses, valves, and plumbing components:
Cryogenic gloves (loose fitting)
Full-face shield or chemical splash goggles
Cryogenic apron
Long sleeve shirt and cuffless pants
Closed toe shoes (no sandals)

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Equipment Usage
Cryogenic containers must be operated in accordance with the manufacturer/supplier
instructions. Cryogenic Dewars must be kept in a well-ventilated area protected from weather
and away from heat sources. In applications that use a modular liquid cylinder as a source of
LN2, the supply will need to be replenished at regular intervals to ensure proper operation of the
freezer.
Recommended First Aid
Every site that stores and uses LN2 should have an appropriate Material Safety Data Sheet (MSDS)
present. The MSDS may be obtained from the manufacturer/distributor. The MSDS will specify the
symptoms of overexposure and first aid to be used. Here is a typical summary. If symptoms of asphyxia
such as headache, drowsiness, dizziness, excitation, excess salivation, vomiting, or unconsciousness are
observed, remove to fresh air. If breathing has stopped, give artificial respiration. CALL A PHSYICIAN
IMMEDIATELY.
If breathing is difficult, supplemental oxygen maybe required. If exposure to cryogenic liquids or cold vapor
occurs, restore tissue to normal, body temperature (37°C) as rapidly as possible, and then protect the
injured tissue from further damage and infection.
Rapid warming of the affected areas is best achieved by bathing it in warm water. The temperature of the
water used should not exceed 40°C. Under no circumstances should the frozen part be rubbed either before
or after warming. If the eyes are involved, flush them thoroughly with warm water for at least 15 minutes.
In case of massive exposure, remove clothing while showering with warm water. The patient should not
drink alcohol or smoke. CALL A PHYSICIAN IMMEDIATELY.

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This manual includes the following symbols.
Table 1: the symbols and their descriptions
Symbol Title Description
Caution
Signifies a CAUTION of potentially hazardous
situation when operating the device that may result in
minor to moderate injury or property damage.
Warning
Signifies a WARNING of a potentially hazardous
situation when operating the device that may result in
serious injury or property damage.
Warning; Low
Temperature
Indicates low temperature or freezing conditions. Take
care to avoid exposure to skin, eyes, and clothing.
Warning;
Asphyxiating
Atmosphere
Indicates the potential for an oxygen-depleted
atmosphere due to nitrogen vapor. Take care to
operate device in a well-ventilated area.
Warning; Electricity
Indicates a potential electrical hazard. Take care to
avoid contact with electricity.
Warning; Explosive
Indicates a potential explosive hazard. The expansion
ratio of liquid nitrogen to gas is 1:700 and can cause
explosive conditions if placed into a sealed container.
Wear Protective
Gloves
Thermal gloves must be worn during indicated
procedures.
Wear a Face Shield
A face shield must be worn during indicated
procedures.
ETL Listed Mark
Chart Fusion Freezer is conformed to UL STD 61010-
1 and certified to CSA STD C22.2#61010-1.
WARNING: Do not modify this equipment without authorization of the manufacturer.

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Display / Control Panel
Table 1: Front Panel Identification
Front Panel MVE FUSION Graphical User Interface (GUI)
Displays Freezer Temperature and Cryogen (LN2) Level % on home screen.
Display A Liquid Crystal Display (LCD) shows the value of all the current conditions
including Freezer Temperature and LN2 level. The display also shows any
current alarm conditions that may exist.
RTD Probe Used to monitor the internal Fusion freezer temperature.
Alarm Visual and
Audible Mutes
Used to silence the visual and audible alarms. Also used to reset the
latching global alarm after the alarm condition is corrected.

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Back Panel / Electrical / Physical Connections
1 RTD Temperature Probe Connection
2 Global Alarm Contacts (These connections monitor All Alarms)
3 Service Serial Port
4 Service Serial Port
5 Input Power
6 Main Power Switch
7 Battery Backup Power Switch
4
3
2
1
6
5
7

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Fusion Dewar Plumbing Connections
Procedure for the First Fill
1. Do not power on the Fusion until all steps are followed.
2. Make sure to have two, 230-liter liquid nitrogen cylinders at 22-35PSI for the first fill.
3. Load racks and empty boxes or alternate inventory systems into the MVE Fusion.
NOTE: VERY IMPORTANT TO ADD RACKS AND BOXES BEFORE FILLING.
CAUTION: Venting may occur which will result in a loss of cryogen inside the pressure vessel
if warm racks are installed after the Fusion’s first fill and/or if more than 1, 2, or 3 warm
racks are installed during its subsequent operation. Be prepared to have a 230-liter liquid
nitrogen cylinder @ 22-35psi to refill pressure vessel if this occurs.
Vent Valve
Pressure Relief Valve
Fill Valve

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See the rack Layout below for reference.
4. Connect one 230 cylinder full of cryogen (LN2) to the Fusions inlet valve using the supplied
transfer hose with the cane oriented in the vertical position, so the pressure relief valve can
vent downwards.
NOTE: One temperature probe will be factory installed in the backside of the Fusion Dewar.
Another aftermarket temperature sensor can be installed in the secondary sensor tube.
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