Millipore EZ-Pak Guide de dépannage
EZ--PakTM
Membrane␣ Dispenser – Distributeur de membranes
Membran–Dispenser – Dispensador de membranas
Dispensatore di membrane – Membrane␣ Dispenser
Operation␣ and␣ Maintenance␣ Instructions – Manuel␣ d'utilisation␣ et␣ d'entretien
Betriebs-␣ und␣ Wartungsanleitung – Instrucciones␣ de␣ utilización␣ y␣ mantenimiento
Manuale␣ operativo␣ e␣ di␣ manutenzione
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http://www.millipore.com
For units produced from February 1997 to
present (cat. no. EZDI SP0 01)
View previous manual on Worldwide Web
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EZ--Pak
Membrane Dispenser
Operation and Maintenance Instructions
ENGLISH
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CONTENTS
Page
1-Introduction 4
2-Component Parts 5
3-Start-up 6 to 8
4-Using the Membrane Dispenser 9
5-Maintenance 10
6-Specifications 10
7-Troubleshooting 11 to 13
8-Replacing Wear Parts 14 to 16
9-Ordering Information 17
10 - Warranty 18
Millipore Offices Worldwide
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Congratulations for your choice of EZ-PakTM membranes and dispenser
for microbiological analysis of liquid samples.
The EZ-Pak system consists of sterile membranes packed in a special
pleated band within a cartridge and specially designed equipment to
allow individual membrane dispensing.
EZ-Pak is designed to be used under standard «clean conditions»
and with traditional membrane filtration methods.
With EZ-Pak, three delicate manipulations are eliminated: opening
individual sterile envelopes, separating the membrane from its protective
papers and removing the fragile membranes from their packaging.
The EZ-Pak cartridges contain 150 black or white gridded 47-mm or
50-mm diameter membranes, and should be used with the MicrofilTM
filtration system for maximum ease of use, speed and cleanliness. Of
course, EZ-Pak is also an ideal complement to traditional filtration
equipment.
1INTRODUCTION
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2COMPONENT PARTS
FIGURE 1 - Assembly and component parts
1- Paper spool
2- Film/paper spool clips
3- Paper guide
4- Drive roller
5- Actuator
6- Plate
7- Separating bar
8- Tensionning spring
(located under the back cover)
9- Film spool
10 - Metal cover
11 - Carrying handle
(moulded in the external case)
12 - Membrane cartridge holder
789210
6
5
4
3
2
1
11 12
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3START-UP
1. Remove the dispenser from its packaging and place it on the work surface
with the dispensing mechanism facing you.
2. Check that all the component parts are undamaged and in place as shown
in Figure 1.
3. Open an EZ-Pak
membranes
cartridge
by detaching the pre-
cut top cover and folding it over
backwards (see Fig. 2).
4. Insert the
cartridge
in the
holder
so that the label is visible (see
Fig. 3).
5.
Important operation:
Fully depress the
actuator
before
proceeding to ensure correct
indexing of the
drive roller.
The
arrow on the
drive roller
must
point vertically upwards.
Caution:
Avoid letting the
actuator
spring back into place.
Slow the movement with the
finger.
6. Remove the
metal cover
by
separating the
cover
tab from
the
plate
tab (see Fig. 4).
Fig.␣ 2
Fig.␣ 3
Fig.␣ 4
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7. Pull out the band until the first
cell containing a membrane is
visible (see Fig. 5).
Note:
The first cells on the band
do not contain membranes.
This
avoids wasting membranes
when installing the band.
8. Introduce the EZ-Pak band
under the
separating bar
. Pull
gently on the band to pass
3␣ empty cells under the
separating bar
.
9. Reinstall the
metal cover
by
inserting the pins in the
housings, then aligning the
cover
tab with the
plate
tab (see
Fig. 6). The locking ball should
audibly «snap» into place.
10. Peel away the transparent plas-
tic film from the paper backing
of the first cells up to the
separating bar.
11. Pull the backing paper through
the mechanism as shown in Fig-
ure 1.
Important operation:
Make sure the red arrow on the
paper after the second fold is
located above the vertical arrow
on the
drive roller.
Engage the
backing paper on the sprockets.
Fig.␣ 5
Fig.␣ 6
Fig.␣ 7
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12. Rotate the
paper spool
until the
clip
is horizontal. Remove the
paper spool clip.
Place the
backing paper on the spool and
secure it with the
clip
. If
necessary, manually rotate the
paper spool
clockwise to take
up any slack.
13. If necessary, slightly pull the
backing paper back from the
cover
toward the
holder
until
there is no slack left in the paper
between the
separating bar
and
the
drive roller.
14. Remove the
film spool clip
, place the end of the
film
on the
film spool
and
secure it with the
clip,
being careful not to twist the film
.
The film should be
firmly held between the
clip
and spool.
Manually rotate the
film spool
clockwise to take up the slack (see Fig. 7).
15. Fully depress the
actuator
once then release it, slowing the upward movement
with the finger.
16. Verify that the membrane band is correctly installed by checking that the
cell dispensed is approximately one-third open (the bluish seal should be
peeled off around one-third of the edge) and that there is no slack in either
the film or the backing paper.
The EZ-Pak membrane dispenser is now ready for use.
Fig.␣ 8
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4USING␣ THE␣ MEMBRANE␣ DISPENSER
1. Position the dispenser on the left-hand side
*
of your workstation with the
Millipore logo directly facing you as shown in Figure 9.
2. To aseptically dispense a membrane, fully depress the
actuator
with your
left* hand. Repeat this step if the cell released does not contain a mem-
brane. The number 001 indicating the first membrane can be seen on the
film above the
separating bar
. During these operations, avoid letting the
actuator spring back into place. Slow the movement with the finger.
3. The membrane will be dispensed horizontally and held in place in the semi-
opened cell (see Fig. 9). Use a flamed and cooled forceps (see Fig.␣ 9) to
remove the membrane with the right hand
*
by pulling it straight out from the cell
and setting it on a Microfil or conventional Millipore filtration support.
4. Proceed with filtration in the normal manner.
5. After dispensing the last membrane, pull off the
clips
and remove the rolls of
used packaging.
* invert if the operator is left handed
Fig.␣ 9
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The case surfaces may be cleaned using a mild detergent or decontaminated
with alcohol. No other maintenance is required.
If the outside case was exposed to UV rays often for relatively long periods, it
may have become slightly dull. Wiping it off with a cloth moistened with alcohol
will restore its original appearance almost completely.
Precautions
Avoid spraying liquids into the mechanism.
Do not autoclave.
Never use the
film spool
to lift or carry the dispenser. Use only the
carrying handle
for this purpose.
If it proves necessary to lubricate certain mechanical parts, use only thick
silicone grease, never oil, and be careful not to allow the grease to come
in contact with the spools and drive belts.
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6SPECIFICATIONS
Materials of construction
External case: Ultem®.
Internal case: Stainless steel.
Paint: Epoxy-baked.
Dimensions and Weight
Height: 240 mm. Width: 160 mm. Depth: 235 mm.
Weight empty: 2.4 kg.
5MAINTENANCE
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