Matrix Impact2 Manuel utilisateur

iii

CONTENTS

Congratulations!..........................................................1

A. INTRODUCTION..........................................................2

B. PIPETTOR OVERVIEW................................................4

B.1 General Description..............................................4

B.2 Keypad................................................................6

B.3 Display..............................................................12

B.4 Specifications ....................................................13

B.5 Hazards and Precautions ....................................14

C. PROGRAMMING AND OPERATION ...........................15

C.1 Programming the IMPACT...................................15

C.2 Programming the IMPACT2.................................19

C.2.1 Program Selection....................................19

C.2.2 Program Recall........................................19

C.2.3 Scratch Pad Mode (Program 0) .................20

C.2.4 Pipetting Speed Adjustment......................20

C.3 Reviewing the Program........................................21

C.4 Programming Examples......................................22

C.4.1 Sample Transfers .....................................22

C.4.2 Incremental Pipetting................................22

C.4.3 Serial Dilutions.........................................23

C.4.4 Simple Dilutions.......................................23

C.5 Operating Procedures .........................................24

D. CALIBRATION ..........................................................26

D.1 Calibrating the IMPACT.......................................27

D.1.1 Volume Measurement...............................27

D.1.2 Pipettor Calibration...................................28

D.2 Calibrating the IMPACT2.....................................30

D.2.1 “EASY” CAL Mode...................................30

D.2.2 “CALC” Mode...........................................31

D.3 CALVIEW..........................................................31

D.4 CALRESET........................................................31

D.5 Recommendations for Accurate Pipetting .............32

CONTENTS (cont’d)

E. TROUBLESHOOTING AND MAINTENANCE................33

E.1 Troubleshooting..................................................33

E.2 Maintenance......................................................35

E.2.1 Pipettor Disassembly ...............................36

E.2.2 Pipettor Cleaning and Autoclaving..............39

E.2.3 Pipettor Reassembly................................40

E.3 Recharging Batteries ..........................................42

E.4 Replacing Batteries ............................................44

F. APPENDICES............................................................48

F.1 Additional Pipetting Examples .............................48

F.1.1 Sample Transfers .....................................48

F.1.2 Incremental Pipetting................................49

F.1.3 Serial Dilutions.........................................49

F.1.4 Simple Dilutions .......................................50

F.2 Chemical Compatibility........................................50

F.3 Customer Service...............................................51

F.3.1 Warranty .................................................51

F.3.2 Customer Service.....................................51

G. IMPACT2STORED PROGRAMS WORKSHEET ................53

Congratulations!

You have purchased the state-of-the-art in precision pipetting. The

IMPACT®is the first cordless electronic pipettor that "understands"

the myriad of liquid handling protocols typically performed in

today's laboratory.

Ergonomically designed and balanced to reduce fatigue, the

IMPACT functions so intuitively, that you may need no more than a

quick survey of this manual to use it.

The IMPACT allows you to accomplish your liquid pipetting tasks

quickly, accurately, and with less risk of developing repetitive

motion related injuries that are typical with manual pipettor use.

Laboratory results will become more standardized, while less time

is expended on tedious bench procedures.

Delivering power, precision, and performance.

is the latest word in liquid handling technology.

Before You Begin

Before using the IMPACT or IMPACT2, verify the contents of your

package. The package should contain the following items:

1IMPACT or IMPACT2Pipettor

1Operator's Manual

2Rechargeable Batteries

1AC Power Transformer

1Warranty Card

1Accuracy/Precision Data Sheet

1Coupon for Free Calibration Service

If you are missing any items, contact your Matrix Technologies

representative.

Note: Batteries must be installed and recharged before using the

IMPACT. Refer to the following sections in this manual:

nSection E.3, Installing/Replacing Batteries

nSection E.4, Recharging Batteries

A. INTRODUCTION

The IMPACT is lightweight and cordless. It provides the

convenience of a manual pipettor, with the programming

capabilities of an electronic pipettor. It runs on rechargeable

batteries and can be used while recharging. The pipettor also has

an automatic shutoff feature to extend battery life.

The IMPACT uses a "paced dispense" feature that performs repeat

pipetting steps while the trigger is depressed.

The IMPACT uses integrated circuit technology to store its

programming information. It will hold up to 40 steps in a program.

The program information is retained in memory, even if the

batteries are fully discharged or removed.

The IMPACT2is the second generation of the IMPACT pipettor

family. It is capable of performing all of the same pipetting

protocols available with the IMPACT, plus a few NEW features. The

expanded capabilities include:

§Expanded memory for storing up to five pipetting programs.

Each program can hold a maximum of 40 steps.

§“Scratch Pad” programming mode which allows for fast and

simple program changes that are not stored into memory.

§Enhanced speed control which offers slower aspirating and

dispensing of viscous liquids. Excellent for Gel loading!

§“EASY” CAL which simplifies in-lab calibration verification by

using distilled water.

INTRODUCTION

The IMPACT and IMPACT2are available in 8-channel and

12-channel formats (see Figure A). An expandable version of the

8-channel format is also available for pipetting samples from test

tubes in a rack. It comes in several models to handle fluid volumes

from 0.5 µl to 1250 µl. The handles are color-coded for easy

identification of different model types (IMPACT vs. IMPACT2) and

different volume ranges. See Section B.4, Specifications.

8-Channel 12-Channel 8-Channel

Expandable

Figure A: IMPACT Configurations

B. PIPETTOR OVERVIEW

B.1 General Description

Keypad

Display

Battery

Case

Pipetting Trigger

Ejector Trigger

Color-coded

Ring

Transformer

Connector

Tip Fittings

Figure B.1: Description of Components

Keypad. The keypad contains the operation keys that are used

in programming the pipetting sequence and volumes. See Section

B.2, Keypad.

Display. The display is an LCD screen that shows the pipetting

operation (fill, dispense, mix, or purge) and fluid volume for each

step in the pipetting program. See Section B.3, Display.

PIPETTOR OVERVIEW

Battery Case. The battery case contains two rechargeable nickel

cadmium batteries. Batteries are charged for 12–14 hours. When

fully charged, the pipettor can perform up to 1200 cycles at full

stroke. Battery life is extended with automatic shutoff after 10

minutes of inactivity. See Section E.3, Installing/

Replacing Batteries.

Pipetting Trigger. The pipetting trigger initiates the pipetting

program. It is pressed when the "Run" or "Purge" command

appears in the display. A single pipetting step is performed by a

quick press and release of the trigger. Repeat pipetting (paced

dispense) is performed by pressing and holding the trigger.

Note: At the end of a paced dispense, the pipet tips should be

removed from the fluid before releasing the trigger, to prevent

aspiration of fluid.

Ejector Trigger. The ejector trigger is pressed to eject the pipet

tips from the pipettor.

Tip Fittings. The tip fittings form an airtight seal with the pipet

tips to ensure accuracy while pipetting.

Note: To extend instrument life, Matrix Technologies has

equipped all IMPACT and IMPACT2pipettors with chemically-

resistant (PEEK) tip fittings. This material allows for pipetting of

acidic solutions without damage to the tip fittings.

Color-coded Ring. The flanged ring around the pipettor handle is

color-coded to identify the pipetting volume of the handle. See

Section B.4, Specifications.

Transformer Connector. The transformer connector receives the

plug from the power transformer for recharging the batteries. The

pipettor can be used while it is recharging.

PIPETTOR OVERVIEW

B.2 Keypad

The keypad contains the operation keys, program keys, and the

special function key.

Display

Operation

Keys

Keypad

Special

Function

Key

Program

Keys

Enter

Fill On Off/

Vol

Purge

Prog

Mix

Disp

Figure B.2a: IMPACT Keypad

Display

Operation

Keys

Keypad

Special

Function

Key

Program

Keys

Enter

Fill On Off/

Vol

Purge

Prog

Mix

Disp

Figure B.2b: IMPACT2Keypad

PIPETTOR OVERVIEW

Operation Keys:

[On/Off] The On/Off key turns the pipettor on or off. When the

pipettor is turned on, it emits a beep tone, flashes

"ZERO" on the display, then automatically resets the

pistons to zero position. After zeroing, the display

shows the first step of the program in the RUN mode.

Note: If the pipettor is being programmed for the first

time, the first step will be the fill operation, with the

default fill volume. See Section C.1, Program-ming

the IMPACT, for the default settings.

[Speed] The Speed key controls the rate at which the liquid is

aspirated or dispensed. The speed setting can be

changed by repeatedly pressing the Speed key.*

For the IMPACT, three speed settings are available:

Speed Setting Display

SLOW SF

MEDIUM SF

FAST SF

For the IMPACT2, five speed settings are available:

Speed Setting Display

SLOW S

MEDIUM SLOW S

MEDIUM S

MEDIUM FAST S

FAST SF

The IMPACT2also features independent speed

adjustment. Speed settings can be changed within a

stored program or during the execution of a program

(see Section C.2.4, Pipetting Speed Adjustment).

*NOTE: IMPACT2speed settings are changed

through the Special Function key (see Special

Function key section on page 11) or by using the

volume key (see Section C.2.4, Pipetting Speed

Adjustment).

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