Linkam Scientific Instruments LTSE420-P Manuel utilisateur
LTSE420-P
Temperature Controlled Probe Stage
USER GUIDE
Linkam Scientific Instruments
2
Contents
Before Setting Up your Equipment…………………………………………………………………………….………..3
Important Notice...................................................................................................................4
Warranty..............................................................................................................................4
Technical Support.................................................................................................................4
Equipment Maintenance........................................................................................................4
Handling Liquid Nitrogen.......................................................................................................4
Feedback..............................................................................................................................4
Safety Precautions................................................................................................................5
Symbol Reference.....................................................................................................5
Introduction..........................................................................................................................6
LTSE420-P Stage Specification...................................................................................6
LTSE420-P System................................................................................................................6
Stage Anatomy.....................................................................................................................7
Lid Assembly.............................................................................................................7
Stage and Heater Assembly…………………………………………………………..…………....………….7
Stage Connectors Assembly…………………….…….…………………...…………………………………..7
LTSE420-PL8 Stage Assembly.....................................................................................8
LTSE420-PB4 Stage Assembly....................................................................................8
Mounting Stage to Microscope with Dovetail Substage.............................................................9
Setting up the Condenser......................................................................................................9
Setting up ECP Water Circulator Pump..................................................................................10
Connecting the Instruments.................................................................................................11
T95 System Controller Cable Connections..................................................................11
LNP95 Cooling Pump Connection..............................................................................11
Remove Transit Screws................................................................................11
Back Panel Cable Connection........................................................................11
LTSE420-PB4 BNC Wire Connection……………………………………………………………….………………….12
Remove the Probe………………………………………………………………………………………………..12
BNC Wire Connection……………………….………………………………………………………….……….12
LTSE420-PL8 Lemo Wire Connection………………………………………………………………………………….14
Remove the Probe………………………………………………………………………………………………..14
Internal Electrical Contact Wiring……………………….………………………………………………….14
Probe Wire Connection……………………………………………………………………………………….…………….16
Adjusting the Probe……………………..…………………………………………………………………………………..16
Probe orientation……………………………………………….………….…………………………………....16
Adjusting the length of the needle…………………………………………………………………..…….17
Bend the tip of the needle………………………………………………………………………….………...17
Change the angle of the needle………………………………………………………………….………….17
Sample Preparation………………………………………………………………………………………………………….18
Cooling Connections............................................................................................................20
Purging Procedure...............................................................................................................21
Use LinkPad to set the LNP95 to Manual Mode..........................................................25
Filling the Liquid Nitrogen Dewar..............................................................................21
Purging the Stage Method 1.....................................................................................22
Purging the Stage Method 2.....................................................................................23
Appendix............................................................................................................................24
Window Assembly...............................................................................................................24
Lid Window Assembly..............................................................................................24
Bottom Window Assembly........................................................................................24
Troubleshooting..................................................................................................................25
Contact Details....................................................................................................................26
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Before Setting Up Your Equipment
Please register your products by going to www.linkam.co.uk and click on the
product/software registration button.
You will need to register your equipment with us to:
Activate your warranty and technical support
Access the online setup videos
Permanently unlock the LINK software (if purchased)
If you have purchased LINK software, please install the software first. This process
will guide to register all of your products.
See LINK manual for further installation instructions.
4
Important Notice
Please check that your Linkam equipment has not been damaged during transit. If there is any evidence of
external damage DO NOT SWITCH ON ANY ELECTRICAL ITEMS.
Contact LINKAM SCIENTIFIC or their appointed distributor immediately. Your warranty may be im-
paired if Linkam is not informed of any transport damage within 7 working days of delivery.
NO attempt should be made to repair or modify the equipment in any way, as there are no user replaceable
parts.
No attempt should be made to open the case except by qualified personnel as hazardous voltages are pre-
sent.
In order to use this equipment successfully, please take time to read this manual all the way through before
using it.
Warranty
This equipment has a warranty against defects in material and workmanship for a period of 12 months.
Linkam will either repair or replace products that prove to be defective. For warranty service or repair,
this product must be returned to Linkam or a designated service facility.
The warranty shall not apply to defects resulting from interfacing, unauthorized modification or misuse,
operation outside of the environmental specifications for the product, or improper site preparation or
maintenance.
Technical Support
Any technical questions or queries should be addressed to the Technical Support Department at the ad-
dress shown on the back of this manual.
Equipment Maintenance
Use a small quantity of isopropyl alcohol with a soft cloth and gently wipe the surface. To clean the stage,
use isopropyl alcohol (IPA) and cotton swabs. Take great care not to touch the platinum temperature
sensor protruding from the side of the heating element. The sensor is very fragile.
Handling Liquid Nitrogen
To cool samples below room temperature a LNP95 liquid nitrogen pump is required. Please refer to your
health and safety manual for instructions on how to handle liquid nitrogen safely. The Dewar supplied
with the LNP95 has a safety release valve built into the siphon assembly. Always use in a well ventilated
room.
Feedback
Your feedback will be greatly appreciated, please go to www.linkam.co.uk to fill in the Feedback form.
5
Safety Precautions
1) Safety Precautions
1) Read this guide before using the equipment. Save these instructions for later use.
2) Follow all warnings and instructions which may be placed on the stage.
3) Never use the equipment if any cables have been damaged. Do not allow any heavy objects to rest
on the cables. Never lay the cables on the floor.
4) Do not obstruct any ventilation holes. Do not attempt to insert anything into these openings. Pro
vide adequate ventilation of at least 75mm all around the equipment.
5) Do not expose the equipment to water. If for any reason it gets wet then unplug it from the mains
and contact Linkam Scientific Technical Support.
6) The equipment is not intended to be used outdoors.
7) If any problems occur then turn all Linkam equipment off and contact Linkam Scientific
Technical Support.
8) Do not remove any covers from the equipment, any servicing should be carried out by qualified
service personnel.
Symbol References
Caution:
This safety symbol is on the back panel of the equipment and warns:-
The user must not make or remove any connections while the unit is powered on.
To avoid electric shock do not remove the cover. Refer servicing to qualified service personnel.
Caution:
This warning symbol indicates that the surface labelled with this symbol may be hot.
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Introduction
Thank you for purchasing the LTSE420-P Probe
Heating and Freezing stage system. Please take
the time to read through the manual as it will help
you to make the most out of the equipment.
LTSE420-P Stage Specifications
Stage:
Maximum temperature: 420°C
Minimum temperature: -196°C with LNP95
Maximum heating rate: 30°C/min
Objective Lens WD: 8.2mm
Condenser lens WD: 12.5mm
Heating surface area: 43 x 53mm
Aperture hole: 2.5mm
Probe:
Maximum voltage: 300V AC
Maximum ampere: 4A
LTSE420-P System
There are two versions of the LTSE420-P stage:
1. LTS420-PL8
2. LTS420-PB4
The LTSE420-PL8 stage is supplied with 2x 4 pin
Lemo connectors and the LTSE420-PB4 stage is
supplied with 4x BNC connectors. Each stage
type is supplied with 4 Probes. The LTSE420-
PL8 can be upgraded to a maximum of 8 Probes.
Each system consists of a LTSE420-P stage, a
T95-LinkPad System Controller with LINK soft-
ware and optional LNP95 liquid nitrogen cooling
pump system for cooling to –196°C.
LINK-DV (digital video) and Linkssy32-AV
(analogue video) video capture software can be
added as an option to control from PC.
Note: please first install the software on the PC
and activate LINK within 10 days.
The LTSE420-P stage is mounted onto the micro-
scope by using either specific stage clamps, an
adaptor plate or by simply placing on the XY
table of the microscope, using double sided adhe-
sive tape.
LTS420-P System with LNP95
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Stage Anatomy
Lid Assembly
Swing the Stage Lid (1) to open the Stage.
1. Stage Lid
2. Lid Insert
3. Viewing Window
4. Hinge
5. Holes for Tube Clip Holder
or window removal tool
Stage and Heater Assembly
6. Stage Chamber
7. Heating / cooling block (43x53mm)
8. Heating element wire
9. Aperture hole (2.5mm)
10. Liquid nitrogen cooling tube
11. PT100 platinum temperature sensor
12. Stage Chamber
13. Lid rubber sealing o-ring seal
Stage Connectors Assembly
14. Bypass Stage body water cooling connector
15. Stainless steel cooling tube for liquid nitrogen
16. Lemo connector for Stage Lead
17. Stage body water cooling connector
18. Gas purge valve
123
Swing Stage Lid to open
5
4
14
15
16
17
18
8
9
7
11
12
6
10
13
8
LTSE420-PL8 Stage Assembly
1. Probe
2. Electrical contact
3. Metal base plate
4. 4 Pins Lemo connector
LTSE420-PB4 Stage Assembly
4. Probe
5. BNC Connector
6. Metal base plate
1
2
4
4
5
4
5
3
6
9
Mounting Stage to Microscope
with Dovetail Substage
The following description is for mounting the
stage on to microscopes which have a circular
dovetail substage assembly (1).
Attach the curved stage clamps (part no. 9548) to
the base of the stage using the supplied hex
screws and the outer most holes in the base plate.
Adjust the two positioning screws (2) so that
approximately 5mm of thread is exposed on the
inside edge of the clamp. This will roughly posi-
tion the stage in the centre of the dovetail.
If you are using the stage in transmission, place
the stage onto the dovetail, then focus a 10X
objective lens on the aperture of the heating ele-
ment. Using the two positioning screws (2)
ensure that the aperture is in the centre of the field
of view and lock the stage in place by tightening
the Locking Thumbscrew (3).
For other types of microscope substage, refer to
the diagram included with the stage adaptor.
Setting up the Condenser
If you are using the stage in transmission, place a
small sample on a microscope slide and place
onto the surface of the microscope slide. Use a
10X lens to focus on the sample. Now close
down the microscope field diaphragm and adjust
the condenser focus so that the edges of the dia-
phragm are in focus. Now use the condenser
positioning screws to centre the condenser in your
field of view. Open the diaphragm so that it just
fills the field of view.
For more information about Koehler illumination
see the extremely informative ‘Microscopy Pri-
mer’ on the Molecular Expressions website.
http://micro.magnet.fsu.edu/primer/
index.html
9542 curved clamps set
2
2
3
Linkam Imaging Station
with dovetail substage
1
10
Setting up ECP Water Circulator
Pump
If you have purchased the ECP with your
LTSE420-P stage, read the following to set up the
ECP (1). Refer to the ECP manual for more
details.
When heating the stage above 300°C for a pro-
long period of time, the metal body of the stage
can get quite hot. The ECP is used to cool the
metal casing. Connect the tubing as shown in the
opposite picture.
Note: If you have an LNP95 Liquid Nitrogen
Cooling system, the tubing from the Dewar must
not be connected to the LTSE420-P stage when
heating above 300°C. The thin black capillary
tube will melt inside the heater and damage it.
1
OutletBypass cooling
Inlet
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