Leica AutoStainerXL Manuel utilisateur

Instructions for Use

Leica AutoStainerXL, V2.3 English – 10/2012

Order-No.: 14 0456 80101 RevC

Always keep this manual near the instrument!

Read carefully prior to operating the instrument!

Leica

AutoStainer XL

Automated slide stainer

Leica AutoStainer XL – Automated slide stainer

The information, numerical data, notes and value

judgments contained in this manual represent the

current state of scientific knowledge and state-

of-the-art technology as we understand it follow-

ing thorough investigation in this field.

We are under no obligation to update the present

manual according to the latest technical devel-

opments, nor to provide our customers with ad-

ditional copies, updates etc. of this manual.

For erroneous statements, drawings, technical

illustrations etc. contained in this manual we ex-

clude liability as far as permissible according to

the national legal system applicable in each indi-

vidual case. In particular, no liability whatsoever

is accepted for any financial loss or consequen-

tial damage caused by or related to compliance

with statements or other information in this

manual.

Statements, drawings, illustrations and other in-

formation as regards contents or technical de-

tails of the present manual are not to be consid-

ered as warranted characteristics of our

products.

These are determined only by the contract provi-

sions agreed between ourselves and our custom-

ers.

Leica reserves the right to change technical

specifications as well as manufacturing pro-

cesses without prior notice. Only in this way is it

possible to continuously improve the technology

and manufacturing techniques used in our prod-

ucts.

This document is protected under copyright laws.

Any copyrights of this document are retained by

Leica Biosystems Nussloch GmbH.

Any reproduction of text and illustrations (or of

any parts thereof) by means of print, photocopy,

microfiche, web cam or other methods – includ-

ing any electronic systems and media – requires

express prior permission in writing by Leica

Biosystems Nussloch GmbH.

For the instrument serial number and year of

manufacture, please refer to the name plate at

the back of the instrument.

NOTE

Published by:

Leica Biosystems Nussloch GmbH

Heidelberger Str. 17 - 19

D-69226 Nussloch

Germany

Phone: +49 (0)6224 143-0

Fax: +49 (0)6224 143-268

Internet: http://www.LeicaB iosystems.com

Instructions for Use V 2.3 – 10/2012

Table of contents

1. Important information .................................................................................................... 6

1.1 Symbols occurring in the text and ............................................................................................6

1.2 Designated use / improper use of the instrument .................................................................. 7

1.3 Qualification of personnel .......................................................................................................... 7

2. Safety................................................................................................................................. 8

2.1 Safety regulations ....................................................................................................................... 8

3. Instrument features ........................................................................................................ 9

3.1 Overview – instrument .............................................................................................................. 10

3.2 Standard delivery - packing list .............................................................................................. 12

3.3 Technical Data ........................................................................................................................... 13

4. Installation ..................................................................................................................... 14

4.1 Site requirements ...................................................................................................................... 14

4.2 Connection.................................................................................................................................. 14

4.2.1 Power .......................................................................................................................................... 14

4.2.2 Water supply .............................................................................................................................. 15

4.3 Battery backup - UPS (optional)..............................................................................................16

4.4 Remote alarm (optional) ........................................................................................................... 17

4.5 Fume control system ................................................................................................................. 17

4.6 Oven ............................................................................................................................................. 17

5. Operation ........................................................................................................................ 18

5.1 The control panel....................................................................................................................... 19

5.2 The main menu........................................................................................................................... 20

5.3 Menu map ................................................................................................................................... 21

5.4 Edit a program ............................................................................................................................ 22

5.4.1 Entering steps ............................................................................................................................ 23

5.4.2 Erasing steps .............................................................................................................................. 24

5.4.3 Inserting a blank step into a program .................................................................................... 24

5.4.4 Removing blank steps in a program ....................................................................................... 25

5.4.5 Saving a program ...................................................................................................................... 25

5.4.6 Deleting a program.................................................................................................................... 25

5.4.7 Copying a program .................................................................................................................... 26

5.4.8 Viewing a program .................................................................................................................... 27

5.4.9 Checking program compatibility .............................................................................................27

5.5 User-adjustable parameters .................................................................................................... 29

Leica AutoStainer XL – Automated slide stainer

Table of contents

5.6 Oven ............................................................................................................................................. 29

5.7 Agitation (Dips) .......................................................................................................................... 30

5.8 Rack movement times ............................................................................................................... 31

5.9 Staining ....................................................................................................................................... 31

5.9.1 Reagent containers ................................................................................................................... 32

5.9.2 Wash system .............................................................................................................................. 32

5.9.3 Water saving .............................................................................................................................. 33

5.9.4 Loading slide racks ................................................................................................................... 33

5.9.5 Unloading racks from the exit drawer.................................................................................... 34

5.9.6 Unloading racks from other stations ...................................................................................... 34

5.9.7 Interrupting staining .................................................................................................................. 35

5.9.8 Aborting a rack .......................................................................................................................... 36

6. Cleaning and Maintenance ........................................................................................ 37

6.1 Cleaning the instrument ........................................................................................................... 37

6.1.1 Wash containers ....................................................................................................................... 37

6.1.2 Reagent containers ................................................................................................................... 37

6.1.3 Slide racks .................................................................................................................................. 37

6.1.4 Oven ............................................................................................................................................. 37

7. Trouble shooting ........................................................................................................... 38

7.1 Instrument failures .................................................................................................................... 39

7.2 Information and warnings ........................................................................................................ 40

7.2.1 During staining ........................................................................................................................... 40

7.2.2 During editing programs ........................................................................................................... 41

7.2.3 During SetUp .............................................................................................................................. 42

8. Warranty and service .................................................................................................. 43

APPENDIX 1 ................................................................................................................... 44

User-adjustable parameters ............................................................................................................... 44

APPENDIX 2 ................................................................................................................... 45

Consumables and accessories ........................................................................................................... 45

APPENDIX 3 ................................................................................................................... 46

Compatible staining programs ............................................................................................................ 46

Glossary .......................................................................................................................... 48

6Instructions for Use V 2.3 – 10/2012

1.1 Symbols occurring in the text and

Warnings and cautions appear in a

grey box and are marked by a

warning triangle .

Notes,

i. e. important information for the

user appear in a grey box and are

marked by an information sign .

Numbers in parentheses refer to

positions in the illustrations or to

the illustrations themselves.

Instrument type:

All information in this Instructions for Use applies

only to the instrument type indicated on the title

page.

A nameplate with the instrument serial number is

fixed on the back of the instrument.

Required information:

For all inquiries it is important to state the follow-

ing:

• instrument type

• serial number.

The Instructions for Use for the Leica

AutoStainer XL includes chapters dealing

with the following subjects:

Chapter 1 Manual structure including:

• Table of contents

• Important information

on this handbook.

Chapter 2 Safety

• Read this chapter before you

attempt to operate the

instrument!

Chapter 3 Instrument features

• General description

• Technical data

Chapter 4 Installation

• Site requirements

• Installation

Chapter 5 Operation

• Control elements

• Menu map

Chapter 6 Trouble shooting

Chapter 7 Cleaning and maintenance

Chapter 8 Warranty and service

Appendix 1 User-adjustable parameters

Appendix 2 Consumables

and accessories

Appendix 3 Compatible staining programs

Glossary

1. Important information

(5)

(Fig. 5)

their meaning

Leica AutoStainer XL – Automated slide stainer

General

This Instructions for Use includes important information related to the oper-

ating safety and maintenance of the instrument.

The Instructions for Use is an important part of the product. It must be read

carefully and completely before setup and first use of the instrument and

must be kept near the instrument at all times.

If additional requirements on accident prevention and environmental pro-

tection exist in the country of operation, this Instructions for Use must be

supplemented by appropriate instructions to ensure compliance with such

requirements.

Be sure to read and comply with the safety instructions,

warnings and cautions in chapter 2, even if you are already

familiar with the operation and use of other Leica products.

1.2 Designated use / improper use of the instrument

- The AutoStainer XL has been designed for staining applications in medi-

cine, biology and industry.

- The instrument may be operated only according to the instructions con-

tained in this manual.

- Any other use is considered an improper use of the product.

1.3 Qualification of personnel

- The AutoStainer XL may be operated only by trained laboratory person-

nel.

- All laboratory personnel designated to operate the AutoStainer XL must

read this Instructions for Use carefully and must be familiar with all tech-

nical features of the instrument before attempting to operate the

AutoStainer XL.

1. Important information

To prevent damages to the instrument and specimens, only

those accessories and spare parts which have been authorized

by Leica may be installed or used with the instrument.

8Instructions for Use V 2.3 – 10/2012

2. Safety

2.1 Safety regulations

This instrument has been built and tested in accordance with the safety

regulations for electrical measuring, control, regulating and laboratory de-

vices.

In order to maintain this condition and ensure safe operation, the operator

must observe all the instructions and warnings contained in this Instructions

for Use.

For current information about applicable standards, please refer to the CE

declaration of conformity on our Internet site:

www.LeicaBiosystems.com

Leica AutoStainer XL – Automated slide stainer

3. Instrument features

The AutoStainer XL is the result of an extensive research program to pro-

vide an innovative stainer which meets the quality requirements of the mod-

ern laboratory, as well as:

• high throughput,

• flexibility,

• safety.

The AutoStainer XL achieves its high throughput by means of an innovative

slide rack transfer mechanism which allows continuous loading of up 11

racks of 30 slides each.

The flexibility of AutoStainer XL also permits simultaneous processing of

slide racks according to different staining protocols so that Papanicolau

and Hematoxylin/Eosin staining can be conveniently performed at the same

time, without reprogramming or reagent changes.

The AutoStainer XL incorporates all of the features which ensure high flex-

ibility, convenience and above all, quality staining. A fan-forced oven is avail-

able to quickly dry slides and optimized wash stations result in rapid re-

moval of excess reagent. The minimum-carryover design of the slide racks

ensures that there are no drips and reagent life is extended.

The AutoStainer XL is safe to use and has an integral fume control system.

Rack loading and unloading is achieved by a unique two-drawer system

which means virtually no exposure to fumes.

The outstanding flexibility, throughput and quality-staining capability of

AutoStainer XL has set a new standard in staining excellence.

Instructions for Use V 2.3 – 10/2012

3. Instrument features

Abb. 1

3.1 Overview – instrument

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