Knauer Azura GPC Manuel utilisateur

The AZURA® GPC Cleanup System

GPC Cleanup (Gel Permeation Chromatography) is primarily employed for general cleanup tasks on a

wide range of sample matrices such as foodstus, tissues, plants and environmental samples. The separa-

tion of components takes place according to their molecular weight due to the heterogeneous pore size

of the stationary phase. High-molecular substances interfering with subsequent analysis are eciently

removed.

Based on the versatile AZURA® device platform the GPC Cleanup System can be customized to fulﬁll spe-

cial requirements.

The AZURA® GPC Cleanup System is operated with Mobile Control Chrom. This intuitive user interface is

run on a tablet directly mounted at the system. It automatically recognizes devices and facilitates system

conﬁguration. Due to block programming methods are created fast with minimal number of clicks. Mobile

Control Chrom makes sample loop loading easily manageable by synchronously switching both valves at

the push of a button.

Installation Guide

Document No. V50043

System layout

Startup

The GPC Cleanup System is set up, installed and commissioned by KNAUER or a company authorized and

contracted by KNAUER.

For detailed information about the operation of the individual devices, please read the corresponding

chapters in the respective instructions.

Note: KNAUER recommends that future users are present during setup and commissioning of the

module, to familiarize themselves with the GPC Cleanup System and its handling.

The layout of the GPC Cleanup System is depicted in the illustration below. The table lists the included

devices and states their functions in the GPC process. See the last page for information about tubing con-

nections.

Legend Device Function

1Eluent tray Storing eluent bottles

2Assistant I

3Mobile Control Chrom Tablet with device control software

416 Port Mulitposition valve Fractionation

5Manual injection valve Sample loop loading or column bypassing/selection

616 Port Mulitposition valves Sample loop selection

7GPC tubing guide

8Fraction tubing guide Sorting fraction tubings

9Sample loop guide Sorting sample loops

bl Eluent tray Storing sample loops

bm Assistant II

bn Detector Detection

bo Pump Eluent delivering

bp Pressure sensor

bq 6 Port 2 Position valve Column bypassing/selection or sample loop loading

}

bn bo

Operating modes

Positions for column bypass / manual injection valve

16 Port Multiposition valve

Inject (2)

Load (1)

6 Port 2 Position valve

Sample loop selection valves / fractionation valve

123

A. Loading samples

1. Set the injection valve to Load (1) position.

2. Address the sample loop you want to load

(start with position 1) by switching the two sam-

ple loop selection valves.

3. Rinse the sample loop tubing with solvent. Op-

tionally empty tubing by injecting air.

4. Load the individual sample through the injec-

tion port of the injection valve with 3x excess of

the sample loop volume.

5. At the end of sample loop loading, switch the

two sample loop selection valves to the next

position. The loaded loop is now closed.

6. Rinse the tubing and valves with solvent. Optio-

nally empty tubing by injecting air.

7. Repeat procedure for all required sample

loops.

8. After sample loop loading: Switch the two

sample loop selection valves to waste (position

16 with short tubing) and rinse with solvent to

remove air.

Note: For each loading, make sure that the

same position is set for both sample loop se-

lection valves. If both valves are not switched

simultaneously, the ﬂow must be stopped.

Otherwise strong pressure spikes can occur

and may cause the pump to stop.

Note: To prevent air bubbles to enter the

sample loop do not remove the syringe

before switching both sample loop selection

valves to next or washing position (position

16 with short tubing).

B. Equilibrating column

Before the sample cleanup the GPC column has to

be equilibrated with the desired eluent. Procedure:

1. Set the injection valve to Load position.

2. Set column bypass valve to Inject position to

integrate the column into the ﬂow.

3. Switch fractionation valve to designated Waste

position.

Note: It is suggested for the column equilibrating

step to use position 16 of the fractionation valve

as waste.

C. Running the GPC

After sample loops have been ﬁlled, the samples

are processed one after the other. Procedure:

1. Set the injection valve to Inject position.

2. Select the individual sample loops by setting

the two sample loop selection valves.

3. Set the column bypass valve to Inject position

to integrate the column into the ﬂow.

4. Switch the fractionation valve to collect frac-

tions.

Tubing connections

Legend

from eluent bottle via pump to pressure sensor

from pressure sensor via manual injection valve (6 Port 2 Position valve)

to sample loop selection valves (16 Port Multiposition valves)

from manual injection valve via column bypass valve (6 Port 2 Position

valve) and column to detector

from detector via fractionation valve (16 Port Multiposition valve)

to fraction bottle

to waste bottle

After installation of single devices according to their respective manual the GPC system ist set up. Lastly,

tubings are connected with the system as highlighted in dierent colours.

Damage to Tefzel® capillary may occur when certain parts are used which are not suitable with Tefzel®. Do

not use stainless steel ferrule 1/16” in combination with stainless steel ﬁtting 12 mm UNF10/32 on Tefzel®

tubings, though both parts are included in the Accessory Kit for 6-Port/3-Channel Injection Valve (order

number F1357).

Component defect

This document is subject to technical changes. Please go to www.knauer.net

for latest version, ordering details and overview of article numbers.

Note: The inlet tubing of the AZURA® Pump P 4.1S included in FPGA is too short for the AZURA® GPC

Cleanup System. Use a longer inlet tubing included in A50041.

Note: In case of a ﬂuctuating detector signal which results from degassing in the ﬂow cell, use a tubing

with a low inner diameter of 0.25 mm between detector and fractionation valve.

Version 2.0

Last update: 2017-09-27

KNAUER Wissenschaftliche Geräte GmbH

Hegauer Weg 38

14163 Berlin, Germany

Tel.: +49-(0)30-809727-0

Fax: +49-(0)30-8015010

E-mail: info@knauer.net

Internet: www.knauer.net

Note: Depending on your preferences, the manual valve and electrical 6 Port 2 Position valve can be

exchanged to function as column bypassing/selection and sample injection.

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