Integra Evolve 3011 Manuel utilisateur

EVOLVE Manual Pipettes Operating instructions

900-00010-00_V00

Declaration of conformity

INTEGRA Biosciences Corp. –

Hudson, NH, USA

declares on its own responsibility that the devices

comply with:

Hudson, New Hampshire, USA - March 15, 2016

Description Models

EVOLVE Manual

Pipettes

3011, 3012, 3013, 3015, 3016, 3018, 3019, 3022, 3024, 3025,

3026, 3032, 3034, 3035, 3036

EU Directives (DoW: Date of Withdrawal) Before DoW DoW After DoW

Restriction of Hazardous Substances 2011/65/EU

EU Regulations

Registration, Evaluation, Authorisation and Restriction of Chemicals

(REACH)

1907/2006

Piston-operated volumetric apparatus - Part 2: Piston pipettes ISO 8655-2

George Kalmakis

VP Operations

EVOLVE Manual Pipettes – Operating instructions V00

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Table of Contents

Chapter 1 Introduction

1.1 Symbols used...................................................................................5

1.2 Intended use.....................................................................................5

1.3 Safety notes .....................................................................................6

Chapter 2 Description of the device

2.1 Scope of delivery..............................................................................7

2.2 Overview of the EVOLVE Manual Pipettes ......................................7

Chapter 3 Installation

3.1 Optional color coded plunger cap.....................................................8

3.2 Changing the Plunger Spring ...........................................................8

Chapter 4 Operation

4.1 Setting the volume..........................................................................10

4.2 Attaching and removing GripTip pipette tips ..................................10

4.3 Pipetting .........................................................................................11

4.3.1 Aspirating and Dispensing ..................................................11

4.3.2 Reverse mode.....................................................................12

4.3.3 Proper pipetting techniques ................................................13

4.4 Troubleshooting..............................................................................14

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Chapter 5 Maintenance

5.1 Cleaning .........................................................................................15

5.2 Single channel lower end disassembly ..........................................15

5.2.1 Disassembly........................................................................15

5.2.2 Reassembly ........................................................................18

5.3 Sterilization.....................................................................................20

5.3.1 Autoclaving the disassembled components........................20

5.4 Servicing.........................................................................................21

5.4.1 Shipping to INTEGRA Biosciences.....................................21

5.4.2 Changing O-rings of tip fittings............................................21

5.4.3 Lubrication ..........................................................................22

5.5 Calibration ......................................................................................23

5.5.1 Materials .............................................................................23

5.5.2 Definitions ...........................................................................23

5.5.3 Test conditions and environment ........................................24

5.5.4 Pre-wetting of tips ...............................................................24

5.5.5 Leak test .............................................................................25

5.5.6 Obtaining the actual volume ...............................................26

5.5.7 Calculation of accuracy and precision ................................27

5.5.8 Adjusting EVOLVE Manual Pipettes ...................................28

5.6 Equipment disposal ........................................................................29

Chapter 6 Technical Data

6.1 Environmental conditions ...............................................................30

6.2 Pipette specifications......................................................................30

6.3 Z Correction Factors.......................................................................32

Chapter 7 Accessories and Consumables

7.1 Accessories ....................................................................................33

7.2 Consumables .................................................................................33

Imprint................................................................................................... 36

EVOLVE Manual Pipettes – Operating instructions V00 Introduction

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1 Introduction

These operating instructions contain all the information required for installation, operation

and maintenance of the EVOLVE Manual Pipettes. This chapter informs about the

symbols used in these operating instructions, the intended use of the EVOLVE Manual

Pipettes and the general safety instructions.

1.1 Symbols used

The operating instructions specifically advise of residual risks with the following symbols:

WARNING

This safety symbol warns against hazards that could result in injury. It also

indicates hazards for machinery, materials and the environment.

It is essential that you follow the corresponding precautions.

CAUTION

This symbol cautions against potential material damage. Follow the

instructions.

NOTE

This symbol identifies important notes regarding the correct operation of the

device and labor-saving features.

1.2 Intended use

EVOLVE Manual Pipettes are intended to be used in a laboratory for aspirating and

dispensing liquids in the volume range of 0.2–5000 µl using GripTip pipette tips.

EVOLVE Manual Pipettes – Operating instructions V00 Introduction

6 INTEGRA Biosciences AG

1.3 Safety notes

EVOLVE Manual Pipettes comply to the recognized safety regulations and is safe to

operate. EVOLVE Manual Pipettes can only be operated when in perfect condition and

while observing these operating instructions.

The device may be associated with residual risks if it is used or operated improperly by

untrained personnel. Any person operating the EVOLVE Manual Pipettes must have read

and understood these operating instructions, and particularly, the safety notes, or must

have been instructed by supervisors so that safe operation of the device is guaranteed.

Repairs may only be performed by INTEGRA Biosciences AG or by an authorized after-

sales service member.

Parts may be replaced with original INTEGRA Biosciences parts only.

Regardless of the listed safety notes, additional applicable regulations and guidelines of

trade associations, health authorities, trade supervisory offices, etc. must be observed.

Please visit our website www.integra-biosciences.com on a regular basis for up to date

information regarding REACH classified chemicals contained in our products.

WARNING

When handling dangerous substances, comply with the material safety data

sheet (MSDS) and with all safety guidelines such as the use of protective

clothing and safety goggles.

CAUTION

Do not immerse the EVOLVE Manual Pipettes in liquid. The fluid can damage

internal parts. Avoid pipetting of liquids whose vapors could attack the

materials PA (polyamide), POM (polyoxymethylene), FPM (fluor-rubber),

NBR (nitrile-rubber), CR (chloroprene), silicone. Corrosive vapors could also

damage metallic parts inside the device.

NOTE

Prolonged exposure of the EVOLVE Manual Pipettes to UV-light can cause

discoloration and/or yellowing of the pipette housing. However, this will not

affect the performance of the device in any way.

EVOLVE Manual Pipettes – Operating instructions V00 Description of the device

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2 Description of the device

2.1 Scope of delivery

• EVOLVE Manual Pipette

• Multipurpose Tool

• Replacement Plunger Cap

• Quick Start Guide

• Replacement O-rings (for 200 μl, 1000 μl and 5000 μl models)

2.2 Overview of the EVOLVE Manual Pipettes

1Plunger Cap

2Plunger, to pipette

3Tip Ejector

4Finger Hook

5Adjustment Dials, to set volume

6Volume Window

The color of the volume indicator label matches

GripTip box insert

7Ejector Sleeve

8Tip Fitting

EVOLVE Manual Pipettes – Operating instructions V00 Installation

8 INTEGRA Biosciences AG

3 Installation

3.1 Optional color coded plunger cap

If desired, replace black plunger cap with optional color coded plunger cap.

3.2 Changing the Plunger Spring

The plunger spring installed inside the pipette is the light spring option. You can exchange

with a heavier plunger spring to achieve more control of the plunger movement.

1) Unscrew the Cylinder Assembly (a) and

separate the Piston Assembly (b, models 2–

200 µl) or Piston (model 1000 µl) from the upper

part of the pipette (c). It is held in place by a small

magnet.

NOTE

5000 µl piston and cylinder assembly is

removed as one piece.

2) Set the pipette to the maximum setting. The

maximum setting is higher than the maximum

volume.

3) Put the two teeth of the Multipurpose Tool (d)

vertically into the slots of the black opening inside

at the bottom of the pipette‘s body (c). Rotate

counterclockwise until the plunger spring

assembly (e) is released.

EVOLVE Manual Pipettes – Operating instructions V00 Installation

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CAUTION

Validate or calibrate your pipette after reassembly.

4) To install the optional spring assembly, screw the

plunger spring assembly (e) clockwise into the

upper part of the pipette using the Multipurpose

Tool.

5) Re-attach the Piston assembly/Piston. Screw

clockwise to attach the Cylinder Assembly (a) to

the body of the pipette.

EVOLVE Manual Pipettes – Operating instructions V00 Operation

10 INTEGRA Biosciences AG

4 Operation

4.1 Setting the volume

4.2 Attaching and removing GripTip pipette tips

CAUTION

To ensure optimal performance of your EVOLVE Manual Pipettes always use

GripTips of correct size which have been designated for the use with the

EVOLVE Manual Pipettes, see “7.2 Consumables” on page 33.

The unique GripTip system of the EVOLVE Manual Pipettes reduces attachment and

ejection forces, ensures a perfect fitting to prevent the tips from falling off and to provide

a perfect seal. On a multichannel pipette all tips sit on the same height.

Attach the tips:

When loading tip(s), press the pipette into the appropriate GripTip(s) until you hear and

feel a click indicating that a seal has been achieved. Once you feel the click, stop applying

pressure. When loading GripTip pipette tips using a multichannel pipette, slowly rock the

pipette from one side to the other side to ensure the proper seal is achieved.

Discard your used tips:

If liquid is in the tips, empty them by pressing down the Plunger (2) to the second stop

(see section 4.3). Tips are easily removed by pressing the Tip Ejector (3).

1) To set the desired pipetting volume press down the Plunger (a)

and rotate slightly clockwise to unlock the volume dials.

2) Spin the Adjustment Dials (b) until the desired volume is

shown in the Volume Window.

3) Slightly rotate and slowly release the Plunger (a) to lock the

volume.

A rim inside the GripTips snaps over the multi-lobes and ensures

firm attachment of the tips.

A shoulder provides a positive stop to prevent over tightening of the

tips. Without hammering, the tip is either on or off but nothing in

between.

O-ring provides forgiving and robust seal surface for the pipette tip.

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