HYRIS BCUBE Manuel utilisateur

HYRIS System™

Quick-Start Guide

(English)

Version 2.1

Hyris Srl

Corso Garibaldi, 60

20121 Milano, Italy

Phone: +39.02.82951302

Mail: office@hyris.net

Other contacts:

Hyris Ltd

Lower Ground Floor, One George

Yard, EC3V 9DF, London UK

Phone: +44.2039361227

Mail: office.uk@hyris.net

Hyris Asia Pac

38 19 Cecil Street

#04-01 - The Quadrant at Cecil,

Singapore (049704)

Phone: +65.8160.7207

Mail: office.uk@hyris.net

Hyris Americas

Los Gatos

Phone: +1 (408) 966-1495

Mail: office.us@hyris.net

HYRIS SystemTM | Quick-Start Guide (ENG)

May 2022

Product info

info@hyris.net

Ordering info

office@hyris.net

bCUBE (REF: bCUBE 2.0)

bCUBE3 (REF: H0001)

bAPP (REF: bAPP, H00001)

bPANEL (REF: H00002)

Visit our website at

www.hyris.net

Hyris Srl

Corso Garibaldi, 60 20121

Milano (MI) Italy

HYRIS SystemTM | Quick-Start Guide (ENG)

May 2022

Reproduction of this document, either in printed or electronics form, is prohibited without the permissionof Hyris S.r.l.

Information in this document is subject to change without notice. Hyris S.r.l. assumes no responsibility for any incorrect

information that may appear in this document.

Notice:

HYRIS S.R.L.DISCLAIMS ALL WARRANTIES WITH RESPECT TO THIS DOCUMENT,EXPRESSED OR IMPLIED,INCLUDING BUT NOT LIMITED TO THOSE OF

MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE.IN NO EVENT SHALL HYRIS S.R.L.BE LIABLE,WHETHER IN CONTRACT,TORT,WARRANTY,OR

UNDER ANY STATUTE OR ON ANY OTHER BASIS FOR SPECIAL,INCIDENTAL,INDIRECT,PUNITIVE,MULTIPLE OR CONSEQUENTIAL DAMAGES IN CONNECTION

WITH OR ARISING FROM THIS DOCUMENT,INCLUDING BUT NOT LIMITED TO THE USE THEREOF.

Trademarks:

HYRIS SystemTM, HYRIS bCUBETM, HYRIS bCUBE3TM, HYRIS bKITTM, HYRIS bAPPTM, HYRIS bDATATM and HYRIS bPANELTM

are a trademark of Hyris S.r.l.

Microsoft® and Windows® are registeredtrademarks of Microsoft Corporation. FAM™, HEX™ and CY5™ are a trademark

of Thermo Fisher Scientific or its subsidiaries in the U.S. and certainother countries.

Technical Support Contacts:

Hyris S.r.l

Corso Garibaldi 60, 20121 Milano(MI) –ITALY

Phone: +39 02 82951302

Mail: support@hyris.net

Website: https://www.hyris.net/

HYRIS SystemTM | Quick-Start Guide (ENG)

May 2022

INDEX

About this Manual 5

HYRIS bCUBETM 6

Packing content.............................................................................................................................................................................6

Hyris bCUBETM Instrument overview.................................................................................................................................................7

Status LEDs...................................................................................................................................................................................8

FIRST SETUP 10

PC settings..................................................................................................................................................................................10

HYRIS bCUBETM Connection...........................................................................................................................................................10

INTERACTING WITH HYRIS bCUBETM: HYRIS bAPPTM AND HYRIS bPANELTM 13

HYRIS bAPPTM interface ................................................................................................................................................................13

System Requirements..................................................................................................................................................................13

Access the HYRIS bAPPTM............................................................................................................................................................13

Create a new analysis...................................................................................................................................................................15

General information 15

Create a new analysis: the samples 18

Create a new analysis: the Cartridge Layout 18

Analysis details 20

GENERAL tab 20

STEPS tab....................................................................................................................................................................................22

STEPS tab –advanced features 23

RESULTS Tab 24

HYRIS bPANELTM interface.............................................................................................................................................................26

System Requirements 26

Installation 26

Main view 26

Settings: Log in/Sync and HYRIS bCUBETM connection 27

New analysis creation 28

Run the analysis 29

Old analyses view 30

HYRIS bCUBETM operative procedure 31

APPENDIX-1: Set HYRIS bCUBETM Wi-Fi from bCUBEs menu 32

APPENDIX-2: Custom Recipe 33

APPENDIX-3: HYRIS Cartridge Preparation 34

Load samples ..............................................................................................................................................................................34

Load the cartridge inside HYRIS bCUBETM........................................................................................................................................34

Removing the cartridge................................................................................................................................................................34

Technical support / Service 36

Support Contacts.........................................................................................................................................................................36

HYRIS SystemTM | Quick-Start Guide (ENG)

May 2022

About this Manual

This manual is referred to bCUBE (REF: bCUBE 2.0) and bCUBE3 (REF: H0001). All references to bCUBE will be referred also

to bCUBE3, unless explicitly specified.

For HYRIS bCUBETM connectivity and functionalities, refer to the Hyris bCUBETM –User Manual document.

For HYRIS bPANELTM offline use, refer to the Hyris bPANELTM –User Manual document.

For the online use of the instrument using HYRIS bAPPTM, please read the HYRIS bAPPTM –User Manual guide.

The information contained in this Guide is proprietary. No part of this document can be copied or reproduced in any form

without written permission of Hyris S.r.l.

The information contained in this manual is subject to change/revision without notice.

This Guide has not to be intended as User Manuals substitute.

In the event the Quick Start Guide should be ruined, incomplete or inadequate, please contact Hyris S.r.l. in order to

immediatelyrestore or replace the incompliant Guide.

The official version of the Quick Start Guide, of which Hyris S.r.l. is directly responsible, is theEnglish version. For countries

in which languages other than Englishare spoken, the official Guide is the one in the Englishversion. Hyris S.r.l. does not

undertake any responsibility for any translations in other languages made by distributors or users.

The observance of the operating procedures and of the warnings described in this Guide is a basic requirement for the

correct working of the equipment and to guarantee the operator’s safety.

The Guide must be read in every part in front of the equipment before using it, in order to become familiar with the

operating procedures, the commands, and the precautions for a correct and safe usage.

The Quick StartGuide should be kept, complete and readable in every part, in a safe place, and, at the same time, it should

be rapidly accessible to the user when using the equipment.

HYRIS SystemTM | Quick-Start Guide (ENG)

May 2022

HYRIS bCUBETM

Packing content

The following items will be found in the provided box:

•HYRIS bCUBETM (Figure 1a)

•Ethernet cable (Figure 1b)

•Power Supply including European and North American power cords (Figure 1c).

Characteristics:

oInput: 100 ~ 240 V c.a. 1.4A 50/60Hz

oOutput: 12V DC 5A

oPower: 60W

•USB stick with HYRIS bPANELTM software installer and User Manuals (Figure 1d)

Note: Please check the presence of all these items. Remove the items carefully from the shipping box and inspect

them for any external damage. If any of the parts is missing ordamaged, contact the manufacturer prior to

installation.

HYRIS SystemTM | Quick-Start Guide (ENG)

May 2022

Hyris bCUBETM Instrument overview

Status LEDs

Access Port

Instrument Base

Upper Venting Holes

Power-on/Power-off

Button

Ethernet Plug

Power Supply

Plug

HYRIS SystemTM | Quick-Start Guide (ENG)

May 2022

Description of the features highlightedin the pictures above:

•Access Port Slider: opened to insert/remove the cartridge inside Hyris bCUBETM. To be kept closed during

operations.

•Access port: insert the cartridge here. Always push the cartridge up to the end of the port.

•Thermal Block: precisely controls the temperature of the cartridge and contained samples. Heating/Cooling is

obtained with a Peltier element.

•Detection block: features highbrightness LEDs withdichroic filters for fluorophores excitation. Detection is

obtained thanks to a CMOS sensor with dichroic filters matched to fluorophores emissionwavelengths.

•Top venting holes: allows air exchange between the inside of the Instrument and the ambient

•Power supply Plug: insert here the 12 VDC adapter jack

•Power-on/Power-off button: press shortly to wake up the instrument from Stand-By. When the machine is

running, long press (about 5 seconds) to safelypower down the bCUBE

•Ethernet Plug: insert here the Ethernet cable for Windows PC / Network communication

•Status LEDs: Give informationabout the status of the instrument

Status LEDs

The status of the bCUBE and its functioning are communicated to the user by the different color combinations of the Status

LEDs on the top of the device.

•bDATA LED: corresponding to the symbol, it indicates the connection status to Hyris server.

Ready color: green

When Ready color is on, HYRIS bCUBETM is connected to HYRIS bDATATM

•Thermal LED: corresponding to the symbol, it indicates if the cartridge is hot or not.

Ready color: orange

When Ready color is on, the internal temperature of the HYRIS bCUBETM is not hot. If

cartridge is inserted, it can be safelyhandled.

•Connection LED: corresponding to the symbol, it indicates the internet connection state of the HYRIS

bCUBETM

Thermal Block

Access Port

Detection Block

Access Port

Slider

HYRIS SystemTM | Quick-Start Guide (ENG)

May 2022

Ready color: blue

When ready color is on, Hyris bCUBETM is connected to a network

For a graphical representation, the symbol (from left to right: bDATA, Thermal and Connection LEDs) will be

displayed in this document when color code explanation is required.

Some examples are reported in the following table:

bDATA

LED

Thermal LED

Connection

LED

HYRIS SystemTM | Quick-Start Guide (ENG)

May 2022

LEDs Colours

DESCRIPTION

INTERPRETATION

•bDATA LED steady green

•Thermal LED steady orange

•Connection LED steady blue

•HYRIS bDATATM connection OK

•Cartridge not hot

•Network connection OK

•bDATA LED steady green

•Thermal LED blinking orange and red

•Connection LED steady blue

•HYRIS bDATATM connection OK

•WARNING: Cartridge hot

•Network connection OK

•bDATA LED blinking green and yellow

•Thermal LED steady orange

•Connection LED blinking red and yellow

•Missing HYRIS bDATATM connection

•Cartridge not hot

•Missing network connection

•bDATA LED blinking green and yellow

•Thermal LED steady orange

•Connection LED steady blue

•Missing HYRIS bDATATM connection

•Cartridge not hot

•Network connection OK. HYRIS bCUBETM

can be used locally

•bDATA LED multicolour

•Thermal LED multicolour

•Connection LED multicolour

•Experiment is running on HYRIS

bCUBETM

•bDATA LED off

•Thermal LED off

•Connection LED off

•HYRIS bCUBETM off

FIRST SETUP

PC settings

PC has to be connected to a stable internet connection.

HYRIS bCUBETM Connection

In order to properly work, the following steps have to be respected:

1. Make sure the Hyris bCUBETM is set on a flat and stable surface

2. Make sure to have correctly provided the condition explained in the PC settings section

3. Connect Hyris bCUBETM and PC to the same LAN according to the following configuration

Note: not all the Status LEDs colour combinations are shown. For further information, please read the

HYRIS bCUBE - User Manual.

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