HARVARD PHD 4400 Hpsi Programmable Manuel utilisateur

PHD 4400 Hpsi Programmable MA1 70-2200

PHD 4400 Hpsi Remote Programmable MA1 70-2201

PHD 4400

Syringe Pump Series

User's Manual

Publication 5406-001-REV-D

EU Directives WEEE and RoHS

To Our Value Customers:

We are committe to being a goo corporate citizen. As part of that commitment,

we strive to maintain an environmentally conscious manufacturing operation. The

European Union (EU) has enacte two Directives, the first on pro uct recycling

(Waste Electrical an Electronic Equipment, WEEE) an the secon limiting the use

of certain substances (Restriction on the use of Hazar ous Substances, RoHS).

Over time, these Directives will be implemente in the national laws of each EU

Member State.

Once the final national regulations have been put into place, recycling will be offere

for our pro ucts which are within the scope of the WEEE Directive. Pro ucts falling

un er the scope of the WEEE Directive available for sale after August 13, 2005 will

be i entifie with a “wheelie bin” symbol.

Two Categories of pro ucts covere by the WEEE Directive are currently exempt

from the RoHS Directive – Category 8, me ical evices (with the exception of

implante or infecte pro ucts) an Category 9, monitoring an control instruments.

Most of our pro ucts fall into either Category 8 or 9 an are currently exempt from

the RoHS Directive. We will continue to monitor the application of the RoHS

Directive to its pro ucts an will comply with any changes as they apply.

• Do Not Dispose Product with Municipal Waste

• Special Collection/Disposal Required

WEEE/RoHS Compliance Statement

Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual

Publication 5406-001-REV-D

Table of Contents

SUBJECT PAGE NO.

General Information:

Manual Description ..............................................4

Warranty ..............................................................4

Repairs ................................................................4

Serial umbers ....................................................4

Calibrations ..........................................................4

General Safety Summary ........................................5

Technical Specifications..........................................6

Theory of Operation ................................................7

Features:

Pressure and Speed ............................................8

Built-in Syringe Table and Custom Syringes ......8

Infusion and Refill Rates......................................8

Target Volume ......................................................8

Auto Fill ................................................................8

Modes of Operation ............................................8

External Connections ..........................................9

onvolatile Memory ............................................9

Stall Detection ......................................................9

Visual/Audible Alarm ............................................9

Power Up Options................................................9

Program Storage..................................................9

User Interface:........................................................10

Description of Keys ............................................11

Entering Data ....................................................12

Special Features Table ......................................12

Initial Setup ............................................................14

Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual

Publication 5406-001-REV-D

Table of Contents

Operation:

Syringe Loading ................................................15

Running the Pump ............................................16

Diameter ............................................................16

Infuse Rate ........................................................16

Refill Rate ..........................................................16

Target Volume ....................................................17

Auto Fill ..............................................................17

Selecting the Run Mode ....................................17

Pro ram Mode:

Program Description ..........................................19

Entering a Program............................................19

Sequence Operation ..........................................20

Program Printout ................................................23

Program Run Time Error Messages..................23

External Control and Interfaces:

RS-232C Devices ..............................................24

Configuring Pump for RS-232C Devices ..........24

User I/O Devices................................................24

Pro rammin Tutorial:

Multiple Infusion Example..................................26

Ramping Up Infusion Rate Example ................27

Multiple Dispensing Example ............................28

Periodic Dispense Loop Example......................29

Combination of Infusion and Withdraw

Profiles Example ................................................30

Use of Events ....................................................31

Pump Chain Commands:

Model 22 Protocol ..............................................33

Model 44 Protocol ..............................................34

SUBJECT PAGE NO.

Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual

Publication 5406-001-REV-D

Table of Contents

Appendices:

A Table of Syringe Diameters ........................45

B Stainless Steel Syringe ..............................46

C ominal Min/Max Flow Rates ....................48

D Custom Applications ..................................48

E Pressure and Force Specifications ............49

F PHD 4400 Hpsi to PC Connection ............49

G RS-232C Specifications..............................50

H Symphony ..................................................50

I User I/O Connector Specifications ............51

J Accessories and Spare Parts ....................51

K Maintenance and Troubleshooting ............52

L 'Auto-Fill' Valves..........................................53

Fi ure 1 Programmable Model Keypad ..............11

Fi ure 2 Rear Panel and Serial umber Label ..14

Fi ure 3 Fuse Replacement................................15

Fi ure 4 Syringe Loading ....................................16

Fi ure 5 Rear Panel External Connections ........24

Fi ure 6 Multiple Infusions ..................................26

Fi ure 7 Ramping Up Infusion Rate....................27

Fi ure 8 Multiple Dispensing ..............................28

Fi ure 9 Periodic Dispense Loop........................29

Fi ure 10 Comb. Infusion & Withdraw Profiles ....30

Fi ure 11 Use of Events........................................31

Fi ure 12 Use of TTL Signal ................................32

Fi ure 13 PHD 4400 Hpsi to PC Connection ......49

Fi ure 14 RS-232C Specifications ........................50

Fi ure 15 User I/O Connector Specifications ......51

Fi ure 16 'Auto-Fill' Valves ....................................53

FIGURE PAGE NO.

SUBJECT PAGE NO.

Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual

Publication 5406-001-REV-D

CCAAUUTTIIOONN:: NNoott oorr cclliinniiccaall uussee oonn hhuummaann ppaattiieennttss..

Warranty and Repair Information

MMAANNUUAALL DDEESSCCRRIIPPTTIIOONN

This manual is designed to provide all operational and program information required to

operate and maintain the PH 4400 Hpsi series pumps. The functions and features are

described in the Technical Specifications section.

WWAARRRRAANNTTYY

Harvard Apparatus warranties this instrument for a period of two years from date of pur-

chase. At its option, Harvard Apparatus will repair or replace the unit if it is found to be

defective as to workmanship or materials. This warranty does not extend to damage result-

ing from misuse, neglect or abuse, normal wear and tear, or accident. This warranty extends

only to the original consumer purchaser.

I O EVE T SHALL HARVARD APPARATUS BE LIABLE FOR I CIDE TAL OR

CO SEQUE TIAL DAMAGES. Some states do not allow the exclusion or limitation of

incidental or consequential damages so the above limitation or exclusion may not apply to

you. THERE ARE O IMPLIED WARRA TIES OF MERCHA TABILITY, OR FIT ESS

FOR A PARTICULAR USE, OR OF A Y OTHER ATURE. Some states do not allow this

limitation on an implied warranty, so the above limitation may not apply to you.

If a defect arises within the two-year warranty period, promptly contact Harvard

Apparatus, 84 October Hill Road, Holliston, Massachusetts 01746 using our toll

free number 1–800–272–2775, or outside the U.S. call 508-893-8999. Email Address is bio-

science@harvardapparatus.com. Goods will not be accepted for return unless an RMA

(returned materials authorization) number has been issued by our customer service

department. The customer is responsible for shipping charges. Please allow a reasonable

period of time for completion of repairs or replacement. If the unit is replaced, the replace-

ment unit is covered only for the remainder of the original warranty period dating from the

purchase of the original device.

This warranty gives you specific rights, and you may also have other rights which vary from

state to state.

RREEPPAAIIRR FFAACCIILLIITTIIEESS AANNDD PPAARRTTSS

Harvard Apparatus stocks replacement and repair parts. When ordering, please describe

parts as completely as possible, preferably using a part number obtained from our

Customer Service department. If practical, enclose a sample part or sketch. We offer a com-

plete reconditioning service.

SSEERRIIAALL NNUUMMBBEERRSS

All inquiries concerning our product should refer to the serial number of the unit, located

on the rear panel (See Figure 2).

CCAALLIIBBRRAATTIIOONNSS

All electrical apparatus is calibrated at rated voltage and frequency. While the flow and vol-

ume will stay calibrated, the peak pressure may vary.

CCAAUUTTIIOONN:: RREEFFEERR TTOO SSEECCTTIIOONN 77,, IINNIITTIIAALL SSEETTUUPP BBEEFFOORREE

PPLLUUGGGGIINNGG IINN PPHHDD 44440000 HHppssii PPUUMMPP..

Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual

Publication 5406-001-REV-D

General Safety Summary

Please read the following safety precautions to ensure proper use of your syringe

pump. To avoid potential hazards and product damage, use this product only as

instructed in this manual.

To Prevent Hazard or Injury:

UUssee PPrrooppeerr LLiinnee CCoorrdd

Use only the specified line cord for this product and make sure line cord is certified

for country of use.

GGrroouunndd tthhee PPrroodduucctt

This product is grounded through the grounding conductor of the power cord. To

avoid electric shock, the grounding conductor must be connected to earth ground.

Before making any connections to the input or output terminals of the product, ensure

that the product is properly grounded.

MMaakkee PPrrooppeerr CCoonnnneeccttiioonnss

Make sure all connections are made properly and securely. Any signal wire connec-

tions to the unit must be no longer than 3 meters.

OObbsseerrvvee aallll TTeerrmmiinnaall RRaattiinnggss

Review the operating manual to learn the ratings on all connections.

UUssee PPrrooppeerr FFuussee

Use only specified fuses with product.

AAvvooiidd EExxppoosseedd CCiirrccuuiittrryy

o not touch any electronic circuitry inside of the product.

DDoo NNoott OOppeerraattee wwiitthh SSuussppeecctteedd FFaaiilluurreess

If damage is suspected on or to the product do not operate the product. Contact qual-

ified service personnel to perform inspection.

PPllaaccee PPrroodduucctt iinn PPrrooppeerr EEnnvviirroonnmmeenntt

Review the operating manual for guidelines for proper operating environments.

OObbsseerrvvee aallll WWaarrnniinngg LLaabbeellss oonn PPrroodduucctt

Read all labels on product to ensure proper usage.

Protective Ground

Terminal

CA TION

Refer to Manual

Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual

Publication 5406-001-REV-D

Specifications

Accuracy ±0.35%

Reproducibility ±0.05%

Syringes (Min. / Max.) 0.5 µ l/140 ml

Syringe Diameter 50 mm

Flow Rate:

Minimum 0.0001 µ l/hr (with 0.5 µ l syringe)

Maximum 220.82 ml/min (with 140 ml syringe)

374.39 ml/min (with 50 mm diameter syringe)

Calibration Automatic

Display 2-line, 40-character fluorescent

Memory Nonvolatile (stores all settings)

Interface RS-232C multiplexed dual bidirectional ports

Connectors:

RS-232C RJ11 4-conductor telephone plug

TTL 9-pin D-SUB connector

Linear Force (Max.) 200 lbs

Drive:

Motor 1.8˚ stepper

Control Microprocessor (from 1/2 to 1/32 microstepping)

Step/Revolution from 800 to 12,800

Step Rate:

Minimum 27.3 sec/step

Maximum 416.7 µ sec/step

usher Travel Rate:

Minimum 0.18 µ m/min

Maximum 190.676 mm/min

Timing Belt Drive 2:1

Lead Screw itch 24 threads/in

ower 100-240 VAC, 50/60 Hz, 75 W, 0.75 A fuse

Dimensions, H x W x D 17 x 23 x 29 cm (6.7 x 9.0 x 11.4 in)

Weight 6.4 kg (14 lbs)

Cable Length (max.) 9.1 m (30 ft) for Remote Model only

15 m (50 ft) for RS-232C

9.1 m (30 ft) for User I/O

Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual

Publication 5406-001-REV-D

Theory of Operation

The PH 4400 Hpsi series employs a microcontroller which controls a small step

angle stepping motor that drives a lead screw and Pusher Block. Microstepping tech-

niques are employed to further reduce the step angle, eliminating flow pulsation. A

keypad is used for entry of operating data to the pump. ata can also be entered via

an RS-232C connector located on the rear panel. The microcontroller, using the inter-

nal Syringe Look Up Table, calculates the cross-sectional area of the syringe selected

and calibrates the flow rate and volume accumulation. The numerous features of the

PH 4400 Hpsi result from the use of microprocessor technology.

The PH 4400 Hpsi Programmable model provides full programmability along with

Infuse/Withdraw capability. Use of the REFILL RATE key, INFUSE/REFILL key, PRO-

GRAM key, AUTO FILL key, Retaining Brackets and Adjustable Stops are all described in

detail in Section 8 of this manual.

Harvard Apparatus PHD 4400 Hpsi Syringe Pump Series User's Manual

Publication 5406-001-REV-D

Features

PPrreessssuurree aanndd SSppeeeedd

PH 4400 Hpsi can deliver up to 220.82 ml/minute with a single 140 ml syringe.

Maximum pressure is dependent on syringe size.

BBuuiilltt--IInn SSyyrriinnggee TTaabbllee aanndd CCuussttoomm SSyyrriinnggeess

The user may select the syringe to be used from the pump’s built-in syringe table.

Syringes are arranged according to manufacturer and material, and then according to

size. The pump will look up and use the diameter for the syringe you select. (See

Appendix A for a listing of the standard syringes.)

If a syringe is to be used, which is not listed in the syringe table, enter the inside

diameter of the syringe in millimeters.

IInnuussiioonn aanndd RReeiillll RRaatteess

Specify independent rates for infusing and refilling. This allows a slow infusion rate

then a fast refill. If Refill Rate is not set, the software defaults to the set Infuse Rate

value.

TTaarrggeett VVoolluummee

Specify the volume that is to be infused or refilled. The pump will run at the rate spec-

ified until this volume has been delivered when in the Volume mode.

AAuuttoo FFiillll

Auto Fill automatically activates an externally attached solenoid (refer to Appendix L

for part number) and refills the syringe when it is empty. This permits infusions to be

virtually independent of syringe capacity.

MMooddeess oo OOppeerraattiioonn

Pump:

Runs continuously in the infuse or refill directions until stopped.

Volume:

Runs until a specified volume has been pumped or refilled.

Program:

Pump operates according to a specified sequence of instructions.

Note: All modes interact with Auto Fill.

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