Instruction Manual
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Dear Customer,
Thank you for choosing a Hanna Product.
Please read the instruction sheet carefully before using the
test kit. It will provide you with the necessary information
for correct use of the kit. If you need additional information,
Remove the chemical test kit from the packing material and
examine it carefully to make sure that no damage has
occurred during shipping. If there is any noticeable dam-
age, notify your Dealer or the nearest Hanna office
immediately.
Each kit is supplied with:
•HI 93707-0 Reagent, packets (100 pcs);
•1 checker disc (containing the 38051 disc);
•2 glass vials with caps;
•1 plastic pipette (3 mL).
Note: Any damaged or defective item must be returned in
its original packing materials.
HI 38051
Nitrite
Test Kit with
Checker Disc
ISTR38051R2 01/05
Nitrites are intermediate oxidation state of nitrogen (in the
oxidation of ammonia to nitrate or in the reduction of
nitrate). Such oxidation/reduction may occur in wastewater
of treatment plants and in natural waters during the
biological decomposition of nitrogen-compounds. In small
quantities nitrites can cause methaemoglobinemia among
infants. Conversely, high levels are required to inhibit
corrosion in cooling towers. Nitrosation reactions of nitrites
can yield organic nitrosamines, which are known to be
carcinogenic.
Note:
mg/L is equivalent to ppm (parts per million).
Range 0 to 0.5 mg/L (ppm) as NO2
--N
Smallest Increment 0.01 mg/L (ppm) NO2
--N
Analysis Method Colorimetric
Sample Size 10
mL
Number of Tests 100
Case Dimensions 235x175x115 mm (9.2x6.9x4.5")
Shipping Weight 446 g (15.7 oz.)
READ THE ENTIRE INSTRUCTIONS BEFORE USING THE KIT
•Use the pipette to fill each glass vial
with 10 mL of sample (up to the mark).
•Insert one of them into the left hand
opening of the checker disc. This is the
blank.
•Add to the other vial 1 packet of HI 93707-0 reagent.
Replace the cap and mix to dissolve.
•Wait for 4 minutes to allow color to
develop. This is the reacted sample.
•Remove the cap and insert
the reacted sample into the
right hand opening of the
checker disc.
•Hold the checker disc so
that a light source illumi-
nates the samples from the
back of the windows.
Nitrites react with chromotropic acid reagent to form a pink
tint in the sample. The amount of color developed is
proportional to the concentration of nitrite present in the
aqueous sample.
•Keep the checker disc at a distance of 30-40 cm (12-
16") to match the color, while having a background
uniform and light in color (e.g. a white sheet). Rotate
the disc while looking at the color test windows and stop
when you find the color match. Read the value in the
result window directly in mg/L (or ppm) of Nitrite-
nitrogen (NO2
--N).
Note: Do not wait more than 6 minutes to perform the
reading.
•To convert the reading to mg/L of Nitrite (NO2
-),
multiply the reading by a factor of 3.29.
•To convert the reading to mg/L of Sodium nitrite
(NaNO2), multiply the reading by a factor of 4.93.
For best results: Perform the reading three times and
take the average value (divide by 3 the sum of the
three numbers). Intensely colored samples will make
the color matching difficult and they should be
adequately treated before performing the test. Sus-
pended matter in large amounts should be removed
by prior filtration.
Caution: Ultraviolet radiation may cause fading of colors.
When not in use, keep the disc protected from light,
in a cool and dry place.
1
0
m
L
The chemicals contained in this kit may be hazardous if
improperly handled. Read the relevant Health and Safety
Data Sheet before performing this test.
Adaptation of the EPA approved method 354.1.
SPECIFICATIONS
SIGNIFICANCE AND USE
CHEMICAL REACTION
INSTRUCTIONS
REFERENCES
HEALTH AND SAFETY
