Gilson Pipetman Fixed Manuel utilisateur

USER'S GUIDE

1 

INTRODUCTION

PIPETMAN®Fixed volume

pipettes are available in 14

dierent models. Together,

they cover a range of vol-

umes from 2 to 5000 µL.

The pipetting mechanism

incorporates a highly pol-

ished stainless steel piston,

a seal, and an O-ring. The

mechanism requires no

routine lubrication.

Tip-ejector and disposable

tipsmeannoneedtohandle

tips, protecting the user

from contamination.

Thetip-holder,theconnect-

ing nut and the tip-ejector

are autoclavable for 20

minutes at 121°C and 0.1

MPa pressure.

CONTENTS page

1  INTRODUCTION 2

2  SPECIFICATIONS 3

3  OPERATION 4

4  USER ADJUSTMENT 5

5  PRERINSING 6

6  PIPETMAN DIAMOND®TIPS 7

7  RECOMMENDATIONS 9

8  TROUBLESHOOTING 10

9  SPARE PARTS 11

EC DECLARATION OF CONFORMITY 13

Push button Tip-ejector

button

Body or

handle

Connecting

nut

Diamond tip

Tip-ejector

Tip-holder

Figure 1

The PIPETMAN Fixed can be easily re-adjusted in

the laboratory, with compensation for variations in

uid viscosity and density. A special calibration key

is provided.

The nominal volume of each

model is shown on the small label

on the push-button.

PIPETMAN Fixed is in accordance

with ISO 8655 Standard.

2  SPECIFICATIONS

PIPETMAN Fixed is a high quality pipette that oers

excellent accuracy and precision. The gures given

in the “Gilson Maximum Permissible Errors” table

were obtained using “PIPETMAN DIAMOND Tips”.

These gures are only guaranteed using genuine

PIPETMAN DIAMOND Tips.

The data given in the table conform to the ISO 8655-2

Standard.

Maximum Permissible Errors

Model Volume Gilson ISO 8655

(Reference) (µL) Systematic Random Systematic Random

error (µL) error (µL) error (µL) error (µL)

F2 (F123770) 2 ± 0.08 ≤ 0.03 ± 0.08 ≤ 0.04

F5 (F123771) 5 ± 0.10 ≤ 0.04 ± 0.125 ≤ 0.075

F10 (F123772) 10 ± 0.10 ≤ 0.05 ± 0.12 ≤ 0.08

F20 (F123604) 20 ± 0.20 ≤ 0.06 ± 0.2 ≤ 0.1

F25 (F123775) 25 ± 0.25 ≤ 0.07 ± 0.5 ≤ 0.2

F50 (F123778) 50 ± 0.40 ≤ 0.15 ± 0.5 ≤ 0.2

F100 (F123784) 100 ± 0.80 ≤ 0.25 ± 0.8 ≤ 0.3

F200 (F123605) 200 ± 1.60 ≤ 0.30 ± 1.6 ≤ 0.6

F250 (F123787) 250 ± 3.00 ≤ 0.75 ± 4.0 ≤ 1.5

F300 (F123788) 300 ± 3.50 ≤ 0.75 ± 4.0 ≤ 1.5

F400 (F123789) 400 ± 3.60 ≤ 0.80 ± 4.0 ≤ 1.5

F500 (F123790) 500 ± 4.00 ≤ 1.00 ± 4.0 ≤ 1.5

F1000 (F123606) 1000 ± 8.0 ≤ 1.3 ± 8.0 ≤ 3.0

F5000 (F123607) 5000 ± 30 ≤ 8 ± 40 ≤ 15.0

Each pipette is inspected and validated by qualied

technicians according to the Gilson Quality System.

Gilson declares that its manufactured pipettes com-

ply with the requirements of the ISO 8655 Standard,

by type testing. The adjustement is carried out

under strictly dened and monitored conditions

(ISO 8655-6):

• Basisofadjustment,Ex.

• Referencetemperature,20°C

• Relativehumidity,50%

• Barometricpressure,101kPa

• Useofdistilledwatergrade3(ISO3696)

• Tenmeasurementsforeachtestvolume.

3  OPERATION

Place a tip on the tip-holder. Press the tip on rmly

using a slight twisting motion to ensure a positive,

airtight seal. (See Chapter 6 for the appropriate tip.)

Always t a tip to your PIPETMAN

before aspirating any liquid.

Aspirating

• Pressthepush-buttontotherst

positive stop (Fig. 2A).

• Holdthepipetteverticallyand

immerse the tip into the liquid

(depth immersion 2-4 mm).

• Releasethepush-buttonslowly

and smoothly to aspirate the

liquid(Fig.2B).

• Waitonesecondandthenwith-

draw the tip from the liquid.

Wipeanydropletsawayfromthe

outsideofthetipusingamedical

wipe.

Avoidtouchingtheoriceofthetip.

Figure 2

Dispensing

• Placetheendofthetipagainsttheinsidewallof

the vessel at an angle of 10 to 40 degrees. Press

the push-button smoothly to the rst stop (Fig.

2C).Waitonesecond.Pressthepush-buttonto

the second stop to expel any residual liquid from

the tip (Fig. 2D).

• Keep the push-button pressed fully down and

(while removing the pipette) draw the tip along

the inside surface of the vessel.

• Releasethepush-buttonsmoothly.

• Ejectthetipbypressingthetip-ejectorbutton.

4  USER ADJUSTMENTS

PIPETMAN Fixed is factory calibrated using distilled

water and very high precision balances.The nominal

valueofthePIPETMANFixedmaybeslightlyadjusted

to compensate for liquids of dierent density or

viscosity.

Adjustment of the factory calibration must

only be performed using a special calibra-

tion key (Figures 3 and 5G).

Thisadjustmenttoaccomodatefordensityorviscos-

ity is limited to one full turn of the calibration key in

either direction, which equals:

± 1.0 µL (models F2 to F20)

± 4.0 µL (models F25 to F100)

± 10.0 µL (models F120 to F200)

± 40.0 µL (models F250 to F1000)

± 200.0 µL (model F5000).

The display is graduated in tenths of the key revolu-

tion (letters A, B,... J) and is read from left to right. Each

small graduation mark represents 0.2 of the distance

between each letter.

To adjust the volume setting to

compensate for a specic density

or viscosity, slide the key over the

push-button until notches t into

the grooves in the calibration sleeve

(Figure3),thenturnthekey:

• clockwisetodecreasethevolume;

• counterclockwisetoincreasethe

volume; go above the desired

value, then decrease the volume

to reach the required setting.

Example: When using a PIPETMAN F100 to aspirate a par-

ticularly viscous solution, you may determine gravimetrically

that the volume delivered is 98.8 µL, and the display reads “E

0.2”. Therefore, you wish to increase the volume dispensed by

1.2 µL. As the interval between each letter on the calibration

displayrepresents0.4µLfortheF100(1/10of4µL),thecalibra-

tion tool must be turned 3 units until the display reads “B 0.2”.

It ispossibletoalterthevolumebymorethanonefull

turn in the counterclockwise direction. In this case,

remember to readjust the same number of turns

when it is necessary to return to the original setting.

Checkthenewvolumegravimetrically.Ifthevolumes

delivered are still not suciently close to the desired

value,makeanotherslightadjustmentintherelevant

direction. Be sure to change tips between volume

settingadjustmentsandtopre-rinseeachnewtip.

When the PIPETMAN Fixed is read-

justed to compensate for a particular

solution, the original setting (ex. 0.2)

can be noted on the self-adhesive

labels supplied (Figure 4).

Liquids of high density, vapor pres-

sure, or viscosity are best pipetted

by the Gilson MICROMAN

positive

displacement pipette.

Figure 3

Figure 4

5  PRERINSING

Some liquids (e.g. protein-containing solutions and

organic solvents) can leave a lm of liquid on the

insidethewallofthetip;pre-rinsethetiptominimize

any errors that may be related to this phenomenon.

Pre-rinsing consists of aspirating the rst volume

of liquid and then dispensing it back into the same

vessel (or to waste). Subsequent volumes that you

pipette will have levels of accuracy and precision

within specications.

This pre-rinsing operation should be repeated when

a new tip is used.

6  PIPETMAN DIAMOND TIPS

PIPETMAN DIAMOND Tips are made to the highest

specications, strict quality control is maintained

throughout the manufacturing process. These tips

are used to calibrate PIPETMAN Fixed, therefore

for optimum performance we recommend using

PIPETMANDIAMONDTipswithyourPIPETMANFixed.

PIPETMAN DIAMOND Tips have the Gilson logo

engraved on their collar, ensuring that you have a

genuine Gilson product. To ensure accuracy and

precision,Gilson'sQualityAssuranceSystemfocuses

on the following critical parameters.

• PIPETMAN DIAMOND Tips are made from pure

polypropylene (virgin, metal-free, to avoid the

possibility of contamination). They are available

sterilizedandwithlters.

• Optimized shape (revised collar for optimum

sealing, thin walls, and ne point), making them

easier to mount, more exible, with no vortexing,

and improved precision.

• PIPETMAN DIAMOND Tips are free from even

microscopic defects, especially at the orice. All

surfaces are smooth and hydrophobic, thereby

avoiding the excessive retention of liquids that

causes poor accuracy and a lack of precision.

• Moldandcavityreferences

are marked on the collar,

ensuringthetraceability.For

qualityassurancepurposes,

batchnumbersappearonall

packages (bags and boxes).

• Theyforman air-tightseal

withthetip-holder,prevent-

ingtheleaksthatcausepoor

accuracy and a lack of precision.

• PIPETMANDIAMOND Tips (except lter tips) may

be autoclaved at 121°C for 20 minutes at 0.1 MPa.

PIPETMAN DIAMOND Filter Tips

Filter tips are used when sample-to-sample, sample-

to-pipette, or sample-to-operator contamination

must be avoided.

Gilson'ssterilizedDiamondFilterTipsarecertiedfree

ofdetectableRNase,DNase,DNA,RNA,andprotease.

PIPETMAN DIAMOND Tips to use for best results

Gilson

Logo

ID Number

Pipette PIPETMAN DIAMOND Filter Tips Type

F2 to F25 DF30ST Sterilized

F50 to F100 DF100ST Sterilized

F200 DF200ST/DF300ST Sterilized

F250 to F1000 DF1000ST Sterilized

Pipette PIPETMAN DIAMOND Tips Type

F2 to F100 D200 Regular

F200 D200/D300 Regular

F250 to F1000 D1000 Regular

F5000 D5000 Regular

To ensure the best performance from your

Gilson pipette, you shoud ALWAYS use

PIPETMAN DIAMOND Tips (in accordance

with ISO8655) to complete your pipetting

system, because PIPETMAN DIAMOND Tips

were used to establish the speciﬁcations.

7  RECOMMENDATIONS

The recommendations below will ensure maximum

performance from your PIPETMAN Fixed.

• MakesuretooperatethePIPETMAN Fixed slowly

and smoothly.

• Whenaspirating,keepthetipataconstantdepth

below the surface of the liquid.

• ThePIPETMAN Fixedshouldbe heldinthe vertical

position.

• Changethetipbeforeaspiratingadierentliquid,

sample or reagent.

• Changethetipifadropletremainsontheendof

the tip from the previous pipetting operation.

• Eachnewtipshouldbepre-rinsedwiththeliquid

to be pipetted.

• Liquid shouldnever enter the PIPETMAN Fixed

tip-holder. To prevent this:

- Press and release the push-button slowly and

smoothly.

- Never turn the pipette upside down.

- Never lay the pipette on its side when there is

liquidinthetip.Useadedicatedstandorhang-

er: Gilson CARROUSEL™, TRIO™, or SINGLE™.

• Nevergreasepistonsorseals.

The pipette can be used between + 4 °C and +

40°Cbutthespeciﬁcationsmayvaryaccording

thetemperature(seeChapter2 for controlled

conditions of use).

• Whenpipettingliquidswithtemperaturesdierent

fromtheambienttemperature,itisrecommended

to pre-rinse the tip several times before use.

• Donotpipetteliquidswithtemperaturesabove

70 °C or below 4 °C.

• After pipetting acids or corrosive liquids which

emit vapors, it is recommended to disassemble

the tip-holder and to rinse the piston, the O-ring

and seal with distilled water.

8  TROUBLESHOOTING

If there is a leak or the pipette is not accurate,

check for the following:

• Theconnectingnutisloose.

Tighten the connecting nut.

• Thetip-holderiscrackedorscored.

Remove the tip ejector and inspect the tip-holder.

For F2 to F20 models, if the tip-holder is damaged,

the piston may also be damaged. Replace the dam-

aged parts (see Chapter 9). When re-assembling the

pipette,theconnectingnutshouldbehandtightened.

• Chemicaldamagetothepistonandseals.

Replace the piston and seals (see Chapter 9). Wash

the inside of the tip-holder using distilled water.

• Improperreassembly.

Disassemble the pipette and reassemble the pipette

correctly.

If there is liquid in the tip-holder, clean the pipette

as follows:

• Removethetipejector.Unscrewtheconnecting

nutandrinsethetip-holder,piston,sealandO-ring

withdistilledwater.Drythesepartsandreassemble

the pipette.

If necessary, the tip-holder, connecting nut and the

tipejectorcanbeautoclavedfor20minutesat121°C

and at 0.1 MPa pressure. The seal and the O-ring

should not be autoclaved.

If there is an air bubble when the sample is

aspirated:

• Dispensethesampleintoitsoriginalvessel.

• Ensurethatthetip isproperlyimmersed inthe

sample liquid.

• Aspiratethesamplemoreslowly.

If the bubble appears a second time, replace the tip.

The outside of the PIPETMAN Fixed can be cleaned

using a cloth dampened with isopropanol.

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