Gilson FC 203B Manuel utilisateur
Gilson,Inc.
3000 Parmenter Street • PO Box 620027 • Middleton, WI 53562 USA
Tel: 608-836-1551 or 800-445-7661 • F 608-831-4451 • www.gilson.com
FC 203B Fraction Collector
Quick Reference Guide
©2016 Gilson, Inc. All rights reserved. LT1732-05
TABLE OF CONTENTS
FC 203B FraCtion ColleCtor |QUICK REFERENCE GUIDE
Table of Contents
Overview.................................................................................. 1
Customer Service................................................................ 2
Notable Features................................................................. 2
Collection Modes ......................................................... 2
Racks ................................................................................ 2
Operation........................................................................ 3
Memory............................................................................ 3
HELP ................................................................................. 3
Cycle Collection............................................................ 3
Collection Windows....................................................4
Key Controls.......................................................................... 5
“Hard” Keys .................................................................... 5
Cycle Collection...................................................................11
Collection Modes................................................................12
Time Mode ....................................................................12
Drop Mode.....................................................................12
Manual Mode ................................................................13
Peak Modes...................................................................13
Racks...................................................................................... 16
Input/Output Control .......................................................18
Overview
1
FC 203B FRACTION COLLECTOR | QUICK REFERENCE GUIDE
Overview
This quick reference guide provides a concise overview
of the operating features of the fraction collector. In most
cases, this is the only documentation needed to operate
the fraction collector.
NOTE
This guide does not detail installation
procedures! Before operating the fraction
collector, refer to the user’s guide to make
all necessary plumbing and electrical
connections.
Use the quick reference guide for information about:
• control keys
• operating modes
• programming
• available racks
• input/output control
For more detailed information, refer to the user’s guide. It
contains instructions for:
• choosing run parameters
• making plumbing and electrical connections
• setting collection windows to selectively save or
discard effluent
• programming the fraction collector to control another
device
• using hidden function keys to set parameter values
and to monitor fraction collector performance
• maintaining and troubleshooting your unit
2CUSTOMER SERVICE |FC 203B FRACTION COLLECTOR
CustOmer serviCe
Customer Service
Gilson, Inc. and its worldwide network of authorized
representatives provide customers with the following
types of assistance: sales, technical support, applications,
and instrument repair.
If you need assistance, please contact your local Gilson
representative. Specific contact information can be found
at www.gilson.com.
Notable Features
Collection Modes
Choose from several collection modes: time, drop,
peak plus time, peak plus drop, or manual. Refer to
Collection Modes on page 12 for more information.
Racks
Choose from a variety of racks. Each rack has a code
number. Tell the fraction collector which rack you
are using by entering the rack code number. When
changing racks, simply enter the new code. See Racks on
page 16 for more information.
3
FC 203B FRACTION COLLECTOR |QUICK REFERENCE GUIDE
CustOmer serviCe
Operation
The fraction collector has two kinds of controls:
• “Hard” keys, such as START, END, YES, NO. The
functions of these keys do not vary.
• “Soft” keys, the four white keys below the display.
The functions of the soft keys vary depending on the
current display. The current function of each soft key
appears on the display directly above the key.
See Key Controls on page 5 for more information.
Memory
The fraction collector remembers its last set of
instructions (e.g., the operating mode and the numerical
values assigned to the various options) even if the power
has been turned off. It always “wakes up” ready to do
exactly what it did the last time.
At each startup, check the list of current values. To check
the values in the current mode, press List.
HELP
Pressing this hard key provides on-line instruction during
setup or during a run.
Cycle Collection
The fraction collector allows for collecting fractions from
each sample into the same set of tubes or into a new set
of tubes.
4CUSTOMER SERVICE |FC 203B FRACTION COLLECTOR
CustOmer serviCe
Collection Windows
Collection windows allow for collecting only the effluent
desired and discarding the rest. Optionally, build up to ten
collection windows.
Collection windows are useful for:
• excluding void volumes
• excluding equilibration or washing volumes
• aiding in selective peak collection
Remember four key points about collection windows:
• It is always possible to collect from start to end,
designating the entire collection period as one
collection window.
• Whenever the fraction collector is outside a collection
window, it automatically diverts the effluent to drain.
• At the start of each collection window, the dispense
head automatically advances to the next tube.
• The collection windows are not mode specific.
Windows created in one mode are active in all other
modes.
5
FC 203B FRACTION COLLECTOR |QUICK REFERENCE GUIDE
Key COntrOls
Key Controls
“Hard” Keys
START
Key used to start collection. This key has the same
function as the Start Soft Key. It is also possible to start
the fraction collector remotely; refer to the user’s guide.
END
A manual override key that ends the collection and
returns the dispense head to the “home” position at the
left and rear of the unit. Typically, the dispense head will
return to “home” after collecting into the last tube in the
rack.
When using the multiple column option, press END after
the last fraction has been collected.
If END is pressed while viewing any display, the ready-
to-run display of the current mode will appear. It is then
possible to begin collecting fractions.
EDIT
Key that calls up the display you use to select or change
collection modes. To access advanced technical and
service options, press EDIT 1, 2, or 3. Refer to the
appendices in the user’s guide for more information
about those options.
6KEY CONTROLS |FC 203B FRACTION COLLECTOR
Key COntrOls
HELP
Displays information and instructions to simplify
operation during setup or during a run. There are many
help messages. Each gives specific information about the
current options.
ADV
Manually advances the number of the current collection
vessel. The current tube number is always shown on the
display. For instance, if tube 1 is the current collection
vessel, pressing ADV makes tube 2 the current collection
vessel. When using the multiple column option, the
current fraction number, rather than tube number, is
displayed.
Typically, tube advance is automatic, but it is possible to
manually initiate an advance by pressing ADV or advance
remotely, as described in the user’s guide.
To understand the interaction between the
ADV and DRAIN Keys, refer to DRAIN below.
DRAIN
Diverts effluent to drain. DRAIN is a two-state key. Press
it once, its light goes on and effluent is diverted to drain.
Press it a second time, the light goes off and the fraction
collector collects into the current vessel.
Whenever the dispense head of the fraction collector
travels from a vessel to the drain trough or back again, it
avoids passing over any vessel into which fractions have
been collected, to prevent contamination of collected
fractions.
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