Fluigent OMI Manuel utilisateur

USER’S

MANUAL

(1) Power supply kit*

O-OMI-SK

*Socket depending on the region

WHAT IS INCLUDED

(1) OMI device

O-OMI-DEV

(1) Tablet

O-OMI-TAB

(3) Cartridge

O-OMI-CART

*Comes in sterile package

(3) Low resistance adaptor

O-OMI-LRCC

*Comes in sterile package

(3) High resistance adaptor

O-OMI-HRCC

*Comes in sterile package

(1) Air ﬁlter & pneumatic kit

O-OMIAF-CTK

(1) OMI connector & tubing kit

O-OMI-CTK

OMI single channel platform [O-OMISC-PTF]

Included in OMI standalone package

PRECAUTIONS

Do not place the product in an unstable location with a level surface

and a strong and stable support

Do not use other power supply than the one provided, it has been

carefully selected to meet the power requirements of Omi in all

conﬁgurations and to comply with all safety standards.

Aria is a perfusion system which automates perfusion or timed

injection protocols. It allows for the sequential delivery of up to

10 different solutions at the desired ﬂow rate into a microﬂuidic

chip, perfusion chamber or petri dish.

Do not open or dismantle the Omi device.

Please refer all servicing to after-sales service

department (support@ﬂuigent.com)

Prevent any objects or liquid from entering the Omi device, this may

cause a short-circuit failure or other malfunction. Failing to respect

this advice would:

• Expose you to direct current/voltage in case the device is under

voltage which may lead to severe damages

• Void device's warranty

• Discharge our company from any liability regarding physical or

device damages.

TABLE OF CONTENT

INTRODUCING OMI 6

SETTING UP 8

P 8

H  9

Power ON / OFF 9

Placing / Removing cartridge and adaptor 10

Pipetting solution 13

Pneumatic ﬁlter / External gas source 13

A  14

Installation 14

Key concepts 15

Registration 16

First login 19

Start protocol 24

ERROR & WARNING LIST 35

EMBEDDED DISPLAY 37

S  37

M  38

W / E  40

P / S  41

R / S  42

E   43

U  44

M  / I  45

A   46

M   47

HARDWARE SPECIFICATIONS 48

TECHNICAL SUPPORT 48

WARRANTY TERMS 49

SAFETY & INFORMATION 50

NOTES 53

6 7

Omi is the ﬁrst automated microphysiological platform, of the peristaltic

pump or syringe pump type, on the market dedicated to to sustain

cell culture or organ on chip model. Flexible, it has been designed

to interface with any type of chip. Compact, transportable (software

on tablet), autonomous (operates on battery), proof incubator, it is

perfectly suited to working conditions in a cell biology laboratory.

INTRODUCING OMI

Omi can be used in single or dual perfusion mode to perform either

single channel perfusion for blood vessel reproduction for example

or co culture for liquid/liquid interfaces for gut on chip or blood brain

barrier reproduction by synchronizing two Omis with one organ on chip.

S  

D  

Omi can be used in single mode to perform for example single

channel perfusion for blood vessel reproduction

Omi can be used

in dual mode to

perform co culture

for liquid/liquid

interface.

Cells

Channel

Flow

Membrane

Cells (type 1)

Channel 1

Flow 1

Cells (type 2)

Channel 2

Flow 2

8 9

SETTING UP

Plug the power source to one of

the two power ports. The battery

will then start charging.

(Note that the battery will then

be able to run)

P ON

SETTING UP

Communication between an Omi device and the mobile application

on your tablet can be done in two ways:

P

H 

Bluetooth: A direct connection between the device and the tablet

is made. The tablet must have its Bluetooth activated and its

geolocation activated as well (this is a current requirement from

Android OS).

Wiﬁ: The device sends its state via a remote server that is forwarding

the information to both the web server and the tablet. Omi devices

must be connected to a Wiﬁ network to make it work.

When Bluetooth is set as the favorite communication method the Omi

devices can still send information via Wiﬁ in parallel. So one method does

not prevent the other. For your information, Bluetooth information is

updated every second for a set of maximum 6 devices in parallel. When

there are more than 6 devices, only the ﬁrst 6 devices can be updated

on such frequency, the others will be updated every 30 seconds.

Aria is a perfusion system which automates perfusion or timed

injection protocols. It allows for the sequential delivery of up to

10 different solutions at the desired ﬂow rate into a microﬂuidic

chip, perfusion chamber or petri dish.

Note: Omi devices cannot connect to a Wiﬁ network protected by a login/

password access (e.g. eduroam) or to a Wiﬁ network using the 5GHz

band. Some places also ask for a MAC-based recognition of the devices

connected to a Wiﬁ network to allow their access to the internet. Check

with your IT department whether this is applicable to your Omi devices.

You can then use the other power port in combination with the Power

Link cable to power up to 4 other Omis in a daisy-chain conﬁguration.

Finally, press the back button for

at least 2 seconds. The screen

should then turn on.

To power Omi OFF, press the button for a few seconds. The screen will

turn OFF. If the power source is plugged, the battery will then still be

charging.

P OFF

Power source Power link cable

Power in

"click"

10 11

SETTING UP

P/R    

SETTING UP

To place a cartridge, slide the two level sensing rods in the cartridge

holes, and gently push it until you hear a double “click”, meaning that

the side bolts locked it.

Pay attention to the cartridge orientation: the rounded notch

should align with the machine.

Check that it is correctly locked by gently trying to pull it out. It

should stay in place horizontally and the two buttons aside should be

oriented inwards.

To unlock the cartridge, push the two aside buttons outwards and

simultaneously. The cartridge should automatically be ejected. You

can then gently slide it out.

When to use the high or low resistance adaptor

1Cartridge holes

2Round alignment notch

3Level sensor rods

4Lock buttons (inward to engage bolt)

Low resistance adaptor can

be used for experiment where

high ﬂow rate is needed. The

low resistance adaptor allows

to reach up to 1ml/min ﬂow rate

with Omi (without taking the

chip design and resistance into

account).

High resistance adaptor can be

used for experiment where low

ﬂow rate is needed. The High

resistance adaptor allows to

reach up to 100µl/min ﬂow rate

with Omi (without taking the

chip design and resistance into

account.

12 13

SETTING UP

When the cartridge is in place, the adaptor can now be positioned.

The adaptor allows the Omi to be compatible with both low and high

resistance Organ-on-chip chips for better versatility.

Low resistance adaptor

High resistance adaptor

Once the adaptor is in place, connect the chip using the provided

adaptors.

P    

To ﬁll the reservoir with a solution once the cartridge is inserted,

remove the cap and use a pipette to transfer the solution.

The volume of liquid in the reservoir should be between 500µl and

4ml. Above or below that, the reservoir will be considered empty or

overﬁlled, which can be a problem

After pipetting, the caps should be closed tightly to ensure there is no

leakage in the system (be sure the cap is equipped with its silicone

gasket).

P     

Filter / External gas

source inlet

SETTING UP

We strongly advise placing the external ﬁlter in order to enhance the

internal pump lifetime.

To use an external gas source (like CO2, N2, or air from outside

the incubator), plug tubing on the luer port on the ﬁlter using an

appropriate luer connector. Please note that the gas source should be

at atmospheric pressure. The internal pump consumes up to 150ml/

min of gas.

14 15

All Omi users are part of

a team. Within this team,

devices, layouts and protocols

will be shared to allow

different members of the

team to work synchronously

when necessary.

Devices designate all Omi apparatus that a user manipulates.

This is basically the hardware that will run your protocols. The list of

your team devices can be seen in “Menu” > “Devices”.

A layout is a virtual group of devices that can be monitored by the

mobile application at a rather high frequency.

While all devices that are part of a layout can be seen and controlled,

a device that would not be part of the current layout will be hidden.

This can be useful to sort your workspace based on the type of

experiments your devices are running or based on their location in

your incubator for instance.

Note that a unique layout can be used for up to 12 devices. Only

6 devices maximum can be monitored simultaneously at high

frequency. The remaining devices will be easily accessible but

will be monitored at a lower frequency (about every 30 seconds in

Bluetooth mode). You can check what is the current layout used by

the application, load another one or create a new one by going to

“Menu” > “Layouts”.

A protocol is a sequence of steps that you want to perform in an

automated way.

When sent to a device, a protocol is automatically started and will

read and execute the protocol sequence, step-by-step. A protocol can

be created or edited via “Menu” > “Protocols”.

The Omi mobile application is available via the Google Play Store of

any Android-based tablet. To ﬁnd it, simply search for “Omi”, access

the application page, check that the publisher is “Fluigent SAS” and

press “Install”.

SETTING UP SETTING UP

A 

I

Use the link below or ﬂash the QR code

to download the application:

https://play.google.com/store/apps/

details?id=com.ﬂuigent.omi&pli=1

K 

Devices LayoutsProtocols

Team

Admin

UserUser

16 17

SETTING UP

Launch Omi mobile application

Open Omi mobile application. On the ﬁrst page, click on register.

On the registration page, ﬁll in the required information.

If all the information is ﬁlled in correctly, a message will pop up to

conﬁrm the Omi's registration and a conﬁrmation link with the account’s

information summary will be sent via email. Click the conﬁrmation link

(or copy-paste the URL in a browser) to verify the email used. Once

done, different features of the mobile application are now accessible.

To use the mobile application, you must create an account. It

requires access to the internet and can be done either via the mobile

application itself or via the Omi website (https://omi.ﬂuigent.com).

To register you will need one of two pieces of information:

The serial number of one of your Omi device, can be found on the

tag below the device:

or a team invitation that someone sent to you to integrate his or

her team.

SETTING UP

R

QR code

containing

the P/N and

S/N

18 19

Launch Omi service web

Open in a browser https://omi.ﬂuigent.com. On the login page (see

below), click on “Register”.

On the registration page, ﬁll in the required information.

Users can login via the web interface before verifying the email used

but will only have access to a very limited set of options that include:

Editing information

Deleting the account

Requesting to resend the conﬁrmation link

SETTING UP

When ﬁrst logging in to the Omi mobile application, users will be

guided through a 4-step tutorial in order to run your ﬁrst protocols.

As a ﬁrst step, a successful login will display the settings page.

Settings

Choose your preferred

communication

method between the

mobile application

and the devices.

Two methods are offered, either a Bluetooth-based communication,

where a direct connection is made via Bluetooth between the device

and the tablet, or a Wiﬁ-based communication, where the device

sends its data to a remote server that forwards it to the tablet. To use

this second method, users must provide the Wiﬁ settings in the next

section (“Omi devices network”). This consists of an SSID and the Wiﬁ

password. Click the button next to the Wiﬁ SSID ﬁeld to scan nearby

networks and select yours. Finally, the Wiﬁ password will always have

to be ﬁlled in manually.

Note that this page also allows users to receive information like your

current team ID, user ID, application version and a direct link to the

web server. It is also possible to change your current team by entering

another team code.

Press “Save” or “Skip” after ﬁlling in the settings to view the second

step of the tutorial.

F  - 

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