Fluigent LineUp Series Manuel utilisateur

USER’S

MANUAL

LINEUPTM SERIES

Push-Pul lPush-Pull

QUICK START GUIDE

LineUp Flow EZTM

Pressure controller

LineUpTM Push-Pull

Pressure controller

Aria is a perfusion system which automates perfusion or timed

injection protocols. It allows for the sequential delivery of up to 10

different solutions at the desired ﬂow rate into a microﬂuidic chip,

perfusion chamber or petri dish.

The quick start guide is a forward to the LineUpTM Series user's manual

A   -  

QUICK START GUIDE

1Supplied (inlet) pressure from pump or compressed air supply

2Power supply to the Flow EZTM / Push-Pull pressure controller

3Regulated (outlet) pressure from pressure controller to reservoir

4Pressurized reservoir P-CAP

5Microﬂuidic set-up in example a microﬂuidic chip

1. The Flow EZTM/ Push-Pull regulates the externally supplied

(inlet) pressuretoauser-deﬁnedpressurewithhighprecision.

2. Thisregulated(outlet)pressurepressurizesthereservoir,

driving the liquid up through the tubing and into the

microﬂuidicsetup.

3. Controllingtheoutletpressureallowsonetocontroltherate

atwhichﬂuidisinjectedintothemicroﬂuidicsetup.

W  

QUICK START GUIDE

Contained in the package

Flow EZTM or Push-Pull Outlet tubing (OD 4mm)

*OD: Outer diameter

P-CAP Kit or Fluiwell Kit (sold separately)

P-CAP (or Fluiwell) reservoir

Fluiwell: for 15mL or 50mL reservoirs

P-CAP for 2mL, 15mL or 50mL reservoirs

Fluidic tubing

Outer diameter and inner diameter

depending on the Kit

QUICK START GUIDE

LineUpTM supply Kit (sold separately)

Inlet tubing (OD: 6mm)

Power supply (24VDC)

Power supply to Sub-D adaptor

*OD: Outer diameter

P 

QUICK START GUIDE

Oncepoweredon,theFlowEZTMor

Push-Pull willdisplaythe "Inlet

pressure check window",givingthe

measuredpressureattheinlet

(P inlet)andthesupplypressure

requiredtooperatethepressure

controller (Ptarget).

For example, left shows a 1000 mbar

range Flow EZTM

P target : 1100 mbar

P inlet : 0 mbar

Note : For the 7000 mbar range as

well as negative pressure ranges

Flow EZTM, this window will not appear.

To wall socket

P 

QUICK START GUIDE

The Flow EZTM or Push-Pull

needs an external pressure

supply to operate. This can be

a lab's compressed air supply,

an air compressor, or any

clean(ﬁltered< 10 µm)anddry

compressed air supply.

Different Flow EZTM ranges

require different supply

pressures(showntotheright).

Note : If one intends to use compressed gas other than air, or if the pressure

supply is out of the pressure range requirements, please contact Fluigent.

Always wear safety protection

when manipulating pressure.

- For positive range, never

exceed 8 bar (120 psi)

- For negative range, never

connectanypositivepressure

Pressure supply

Flow EZTM

range (mbar)

Required pressure

supply (mbar)

7000 7100

2000 2100

1000

1100

345

150

-25

-800

-69

-345

-800

The Push-Pull module works the same way as other Flow EZTM modules,

except that it can output pressure (above atmospheric pressure)

and vacuum (below atmospheric pressure) through the same port.

P-P 

Note : Depending on the supply pressures, the performance of

the module can vary, especially for the vacuum pressure supply.

When connecting the two supplies, only the positive pressure supply

will be checked on the "Inlet pressure check window". (see page 10)

Push-Pull output (mbar) Required pressure supply (mbar)

1000 1100

-800 -800

QUICK START GUIDE

Positive pressure

supply inlet

Vacuum

supply inlet

Pressure /

Vacuum outlet

To do so, it needs two inlets: the positive one, uses the standard pressure

supply of the Flow EZTM, and the negative one, utilizes an additional

connector located at the front between the FLOW UNIT port and the

"unlock" handle.

Ensure to power ON the LineUpTM Push-Pull and set the pressure

value on 0 mbar before connecting the vacuum supply.

Special attention

should be taken to

preventliquidfrom

ﬂowing back into

the module.

QUICK START GUIDE

A Push-Pull module can be combined with other positive

pressure LineUPTM modules the same way as a Flow EZTM

1 bar. If connecting to higher pressure modules, please

consider adding an Adapt module to reduce the

positive pressure supply. (See page 29 for more details)

When using a Push Pull module, care should be taken to

avoid drawing liquid into the system. This can seriously

damage the module. It is advised to use a liquid filter

between the outlet and the reservoir as it reduces the risks.

Note: The pressure supply is mandatory for the use of the Push-Pull even

in vacuum mode only. The vacuum supply is optionnal.

Please, never stack a Push-Pull module with a negative Flow EZTM as it

would not work.

A   

(A 1000 mbar range Flow EZTM is shown as an example)

Adjust the pressure

from your

pressure source

The P inlet value

is displayed in

red

The P inlet value

is displayed in

green

When connecting the pressure supply, if its value (P inlet) is not set at

the required value (P target), the Flow EZTM or Push-Pull will continue to

display the "Inlet pressure check window" (with the exception of the 7000

mbar range and the negative ranges which don't have this window)

If the value is displayed in red, please adjust the pressure supply.

When the two values are close enough, the P inlet value will turn green

and the display on the Flow EZTM or Push-Pull will transition to "Operation

window"

QUICK START GUIDE

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