Evolve 70 Manuel

70mm, 100mm and 140mm

Chromatography Columns

DELTA PRECISION LTD

Chromatography Column

Operating Manual and Installation Guide

70mm, 100mm and 140mm Columns

Revision History

Revision

Comments

Date

Initial release

21/11/2014

Photos updated

25/02/2015

Address Updated

21/06/2018

Pressure Equipment Directive updated

06/04/2021

Technical specification updated caster not available on 70-140mm columns

22/04/2021

Updated to reflect Evolve® Trademark status

12/07/2021

Evolve® is a registered trademark of Delta Precision Ltd. Evolve® is a registered trademark in the

EU, UK and US.

Manual Revision

Date

July 2021

Table of Contents

Column Hardware .......................................................................................................... 1

Mechanical Description ........................................................................................................................... 1

Technical Data. ...................................................................................................................................... 3

Weights and Dimensions ........................................................................................................................ 3

Pressure Drop ........................................................................................................................................ 4

Material Conformity ............................................................................................................................... 6

Chemical Compatibility .......................................................................................................................... 7

Safety Guidelines ............................................................................................................ 9

Pressure Equipment Directive (PED). ................................................................................................... 9

Over-pressurization ................................................................................................................................. 9

Handling ................................................................................................................................................ 9

Chemical compatibility .......................................................................................................................... 10

Installation and Column Set-up ................................................................................... 11

Installation. .......................................................................................................................................... 11

Column Operation ........................................................................................................ 15

Hydrostatic Leak Test ......................................................................................................................... 15

Media Preparation ............................................................................................................................... 16

Column Packing (Constant Flowrate Method) ...................................................................................... 16

Unpacking ........................................................................................................................................... 18

Cleaning and Storage ............................................................................................................................ 18

Maintenance ................................................................................................................. 20

Routine Maintenance Procedures-General guidelines .............................................................................. 20

Disassembly of 70mm, 100mm and 140mm Columns ......................................................................... 21

Reassembly of 70mm, 100mm and 140 column. ................................................................................... 24

Troubleshooting ........................................................................................................... 27

Leakage from adjuster seal .................................................................................................................... 27

Leakage from fixed bed support seal ..................................................................................................... 27

Leakage from inlet or outlet .................................................................................................................. 27

Excessive column back pressure ............................................................................................................ 28

Media Leakage .................................................................................................................................... 28

Kits, Spare parts and Accessories ................................................................................. 29

Spare parts and kits ............................................................................................................................. 31

Spare Parts and kits Cont. ................................................................................................................... 32

Accessories ............................................................................................................................................ 34

Contact Us .................................................................................................................... 35

Appendix 1 Chromatography Performance .................................................................. 36

Performance Measurement and Column Efficiency ................................................................................. 36

Performance Results From Evolve®Columns ........................................................................................ 38

Table of Figures

Figure 1.1 Evolve®Column Range 70mm, 100mm, 140mm, 200mm and 250mm columns .................... 2

Figure 1.2. Pressure Drop Evolve®70mm column ..................................................................................... 4

Figure 1.3 Pressure Drop Evolve®100mm column ................................................................................... 4

Figure 1.4 Pressure Drop Evolve®140mm column ................................................................................... 5

Figure 3.1 Diagram showing the location of the fixed bed support ............................................................. 11

Figure 3.2 Photograph showing the installation of the fixed bed support .................................................... 12

Figure 3.3 Diagram showing the location of the adjuster bed support ........................................................ 13

Figure 3.4 Photograph showing the location of the adjuster bed support ..................................................... 13

Figure 4.1 Schematic of the column with part identification ....................................................................... 17

Figure 5.1 Adjuster Assembly Evolve® 100 column .............................................................................. 22

Figure 5.2 Fixed Assembly Evolve® 100 column ................................................................................... 23

Figure 5.3 Diagram showing the location of the fixed bed support ............................................................. 24

Figure 5.4 Photograph showing the installation of the fixed bed support .................................................... 24

Figure 5.4 Photograph showing the location of the adjuster bed support ..................................................... 25

Figure 5.5 Diagram showing the location of the adjuster bed support ........................................................ 25

Figure 5.6 Photograph showing the final location of the adjuster bed support ............................................. 26

Figure 5.7 Photograph showing the alignment of marking on flowtube and adaptor to ensure component is

correctly tightened ....................................................................................................................... 26

Figure A1 Description of Column Efficiency Calculations ........................................................................ 37

Figure A2 Example of typical chromatogram. Evolve®250mm column. 2% Acetone sample, flowrate

100cm/hr, bed height 19.5cm. ................................................................................................... 38

Column Hardware

Mechanical Description

The Evolve®Column is designed specifically for use in the Biopharmaceutical Applications. The

column can be used in pilot or production scale Chromatographic processes.

A pressure rating of 4 bar, operating temperature range of 2-30OC and adjustable bed height

makes this column suitable for a wide range of chromatography media types and chromatographic

techniques.

The columns have the following design features.

The entire flowpath is non-metallic, which has the following advantages:

• Eliminates the corrosion risk and contamination of the process

• Allows the use of high concentration sodium chloride solutions and Guanidine

hydrochloride.

• Prevents any product inactivation through metal contact

Unlike other columns available on the market the Evolve®columns have an integral bed

support and seal assembly. The polypropylene mesh and seal are manufactured as a single

unit and fits directly on the flowcell without any fixings.

This has the following advantages.

• Ensure that maximum area is available to flow, promoting good chromatographic

performance and cleanability.

• Eliminates use of fixings to secure the bed support to the flowcell, removing the potential

for leak paths or dead flow zones.

• Reduces number of components in the column assembly, leading to easy operation and

maintenance.

Sealing of the adjuster flowcell to the tube wall is achieved by a moulded Santoprene seal.

Central activation of this seal ensures that compression is even. The mechanism to energise

and de-energise the seal is simple and easy to operate.

Positioning of the adjuster assemble within the tube is easy and simple using the central

adjustment mechanism.

Chapter

The column is composed of three main sub-assemblies:

• Adjuster assembly- consisting of the adjuster flowcell assembly, adjuster seal

components, adjuster flange and a central height adjust rod. This allows the

position of the adjuster to be varied using the height adjust rods over the range

20-520mm.

• Tube unit – consisting of the column tube, flanges and column stand or support

legs

• Fixed assembly – consisting of the bottom flowcell assembly and flowtube.

Figure 1.1 Evolve®Column Range 70mm, 100mm, 140mm, 200mm and 250mm columns

Technical Data.

100

140

200

250

Cat No.

AB070505

AB100505

AB140505

AB200505

AB250505

Tube id mm

100

140

200

250

Cross Sectional area cm2

38.5

78.5

154

314.2

490.9

Maximum Bed height mm

520

Minimum Bed height mm

Max Column Volume L

16.3

25.5

Adjustable Capacity L

1.9

3.9

7.7

15.7

24.5

Pressure rating bar

psi

Operating temp

2-30oC

Bed support pore size µm

8µm

Weights and Dimensions

100

140

200

250

Cat. No

AB070505

AB100505

AB140505

AB200505

AB250505

Adjuster Weight kg

7.5

12.7

Total Weight empty

11.5

12.5

45.5

Total Weight Full*

Maximum Diameter

ex casters mm

226

282

455

495

Maximum Diameter

inc casters mm 1

455

495

Maximum height mm

1523

1543

1545

1550

Minimum

Clearance** mm

1620

1750

1 casters are not available on 70-140mm columns

*based on bed height of 520mm and specific gravity 1.05,

**minimum height required to remove adjuster from tube unit.

Pressure Drop

The pipework and column contribute to the pressure drop of the chromatographic system.

Measuring the pressure drop from the column inlet to the column outlet shows the

contribution made by the column hardware. The tests were performed at ambient

temperature 15-20oC using DI water.

Figure 1.2. Pressure Drop Evolve®70mm column

Figure 1.3 Pressure Drop Evolve®100mm column

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0500 1000 1500 2000 2500

Linear Velocity cm/hr

Pressure bar

0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6

Volumetric Flowrate L/min

Evolve 70

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0200 400 600 800 1000 1200

Linear Velocity cm/hr

Pressure bar

0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6

Volumetric flowrate L/min

Evolve 100

Figure 1.4 Pressure Drop Evolve®140mm column

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

0500 1000 1500 2000 2500 3000

Linear Velocity cm/hr

Pressure bar

0 0.5 1 1.5 2 2.5 3 3.5 4

Volumetric flowrate L/min

Evolve 140

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