Edvotek EDGE Manuel utilisateur

Features:

• Integrated power supply: 100V or 150V selectable output

for results in as little as 10 min.

• Safe, high-intensity blue LED illumination: 470 nm wave-

length is optimized for SYBR® Safe and compatible with

other popular nucleic acid stains.

• Convenient 10 x 7 cm gel tray with two dual-sided

combs for up to 16 samples per gel

• Flexible gel volumes: 30-50 mL

• Built-in timer: 1-99 minutes

• Fan and ventilation system minimizes condensation

during experiments

• Convenient hinged lid contains dual automatic power

cutoswitchesforsafety

• Stainless steel and platinum electrodes are compatible

withallstandardelectrophoresisbuers

• 17 x 17 x 15 cm

Voltage

Selector Timer

Screen Increase/

Decrease

Timer

Start/Stop

Button

Visit our website at www.edvotek.com

OPERATION MANUAL EDGE™Integrated Electrophoresis System

Introduction:

The Edvotek® EDGE™ Integrated Electrophoresis System combines an electrophoresis apparatus, power

supply, and blue light transilluminator into a single, user-friendly unit.

Warnings:

• Useonlyappropriateelectrophoresisbuersin

thechamber.Improperbuerscanleadtodam-

age to the power supply and electrodes and can

risk electric shock to the user.

• DO NOT open the outer housing of the EDGE,

modify, or circumvent the safety features of the

unit. This product should only be disassembled or

serviced by properly trained professionals.

• While the light wavelengths emitted by this prod-

uct do not require specialized eyewear, the blue

light is high intensity. DO NOT stare at the blue

lights for a long period without the orange lid in

place.

• DO NOT submerge the outer housing of the

EDGE™ or pour liquids onto the unit.

• Wear gloves and follow all manufacturer recom-

mendations when working with DNA stains

• At all times, USE COMMON SENSE.

Each EDGE™ Includes:

(1) EDGE™ base unit with integrated 100V and 150V power

supply, hinged lid, and blue light transilluminator.

(2) EDGE™ buer tank with stainless steel and platinum

electrodes.

(3) EDGE™ accessories: 10 x 7 cm gel tray, two dual-sided

combs (6- and 8-well) and two casting endcaps.

The EDGE™ has been designed for safety and convenience. A

hingedlidanddualsafetycutoswitcheshelptopreventim-

proper use and minimize the risk of electric shock. The blue

light transilluminator allows users to monitor DNA migration

throughout the experiment using SYBR® Safe or similar

stains, without the hazards associated with UV light.

(1)

(2)

(3)

A built-in power supply allows users to select between 100V and 150V, producing results in as little as 10 minutes.

Theintegratedtimercanbesetbetween1and99minutestoautomaticallyturnothepower.Gelscanbecast

with one or two combs, providing up to 16 wells in a single run.

User Maintenance:

Before cleaning your unit, ALWAYS disconnect the

cord to prevent electric shock. The EDGE™ base can

be cleaned by wiping with a lightly damp, soapy cloth.

Care should be exercised to prevent water from

running inside the unit. Do not use abrasive cleaners

orstrongsolvents.Thebuerchamber,tray,combs,

and endcaps can be fully submerged to clean with mild

detergent if necessary. Avoid direct contact with the

positive and negative electrodes. Dry everything fully

before storage.

In the unlikely event that you experience any problems

with your unit that cannot easily be remedied, please

contact EDVOTEK® to explain the problem and obtain a

Return Goods Authorization #. After obtaining the RGA

#, return the unit if necessary and include any details of

the fault observed. Remember to return the unit in its

original packing. EDVOTEK® accepts no responsibility

for damage to units that are not properly packed for

shipping.

1.800.EDVOTEK • www.edvotek.com • [email protected]

OPERATION MANUAL EDGE™Integrated Electrophoresis System

Instructions:

CASTING AN AGAROSE GEL

Note: Always follow the instructions for your experiment to determine the concentration of agarose required for your

specic samples.

1. PREPAREtheagarosegelsolutionbycombiningagarosepowderandelectrophoresisbueraccordingtothe

recipe in your protocol and microwaving until fully dissolved. The EDGE™ is optimized for 30-50 mL gels.

2. COOL the agarose solution to 60 °C with careful swirling to promote even dissipation of heat.

3. While the agarose is cooling, SEAL the ends of the gel-casting tray with the rubber end caps. PLACE the com-

b(s) into the appropriate notch.

4. Follow the instructions provided with your experiment to ADD SYBR® Safe stain to the cooled agarose solu-

tion. Note: For general experiments we recommend a 1:10,000 to 1:20,000 dilution of SYBR® Safe.

5. POURtheagarosesolutionintothegel-castingtrayandwaituntilthegelhassolidied.Mostgelswillbe

ready to use within 15 minutes.

6. REMOVE the end caps and comb from the tray. Take care when removing the comb to prevent damage to the

wells. The gel is now ready to use.

RUNNING AN AGAROSE GEL IN THE EDGE™

1. PLACEthegel(onthetray)intotheelectrophoresischamberandensurethechamberisplacedrmlyintothe

EDGE™base.Thechambershouldsitrmlyontothebasewithbothelectrodestouchingthemetalcontacts

onthebase.TheelectrophoresischambershouldsitushagainstthebottomoftheEDGE™baseunit.

2. COVERthegelwith1xelectrophoresisbuer,beingcarefultonotoverllthechamber.Forbestresultsthe

buershouldbeapproximately0.5cmabovethesurfaceofthegel.Ensurethatthegeltrayisalignedinthe

center of the chamber.

3. LOAD the DNA samples into the wells according to the experiment protocol.

4. LOWER the orange safety lid and check that is it tightly closed.

5. Using the arrow buttons, ADJUST the timer to reach the desired value. For most experiments we recommend

not exceeding 30 minutes before checking on the progress of your samples.

6. SELECT the desired voltage, 100V or 150V using the voltage selector buttons. An orange LED will indicate

which voltage has been selected.

7. PRESS the START/STOP button to begin the run. Bubbles will form near the electrodes. The power will not

turn on if:

• The cover is not correctly placed on the base

• The electrodes in the chamber are not making contact with the EDGE™ base

 • Thebuerinthechamberisincorrectorthebuervolumeistoolow.

8. At any time during the experiment, PRESS the ON/OFF paddle at the base of the EDGE™ to illuminate the gel

using the blue LEDS.

9. After electrophoresis is complete, PRESS the START/STOP button to stop the current, document the results of

theexperiment,andthenopenthelidtodisposeofthegelandelectrophoresisbuer.

DOCUMENTING YOUR RESULTS

At any point during or after the experiment has completed, the results can be documented by using a camera

or smartphone to take an image through the orange safety lid. Dimming the lights in the room can increase the

visibility of DNA if needed.

Visit our website at www.edvotek.com

OPERATION MANUAL EDGE™Integrated Electrophoresis System

Specifications:

• LED Wavelength: 470 nm +/- 10 nM

• LED Life: 50,000 hours

• Input voltage: 100-240V AC, 50/60 hz

• Output voltage: Selectable at 100V or 150V DC

• Dimensions: 17 cm (w) x 17 cm (l) x 15 cm (h)

• Operating conditions: 4 °C to 37 °C,

75% maximum humidity

Guarantee:

The unit is guaranteed against any defect in material or

workmanship for three years. The warranty period is from

the date of receipt, and within this period all defective

parts will be replaced free of charge provided that the

defect is not the result of misuse, accident or negligence.

Servicing under this guarantee should be obtained from

EDVOTEK®.

Notwithstanding,thedescriptionandspecication(s)ofthe

units contained in the User’s Manual, EDVOTEK® hereby

reservestherighttomakesuchchangesasitseesttothe

units or to any component of the units. This Manual has

been prepared solely for the convenience of EDVOTEK®

customers and nothing in this Instruction Book shall be

taken as a warranty, condition or representation concern-

ingthedescription,merchantability,tnessforpurposeor

otherwise of the units or components.

Contact Information:

For technical, sales or servicing information, contact:

EDVOTEK, Inc.,

1121 5th Street NW

Washington, DC 20001

1.800.EDVOTEK (1.800.338.6835)

Fax: 202.370.1501

[email protected] • www.edvotek.com

Troubleshooting:

The EDGE™ does not power on:

• Check that the power cord is fully plugged into the

EDGE™ base and that the cord and outlet are func-

tional.

• Conrmthatthepowerswitchontherearofthe

EDGE™ base is turned on.

• Inspect the fuse and replace if needed. The fuse is

located directly under the power plug on the rear of

the EDGE.

The START/STOP indicator LED does not stay lit and/or

the experiment does not run:

• Ensure that the lid is fully closed.

• Pressrmlyontheelectrophoresistanktoensure

thatitisrmlyseatedandthatbothelectrodesare

making contact with the base.

• Checkthatthecorrectbuerhasbeenusedandthat

thegelisfullysubmergedinbuer.

• Ensure that the timer has not reached zero. Increase

the remaining time and try running the experiment

again.

The gel tray will not t into the chamber:

• Thetrayisdesignedtotonlyoneway.Rotatethe

tray180˚andtryagain.

• TheEDGE™usescustom10x7cmtraysthatdier

from existing Edvotek electrophoresis accessories.

Please ensure that you are using the proper electro-

phoresis trays.

No DNA bands are present on the gel:

• Check that the blue light is turned on by pressing the

paddle switch

• Ensure that SYBR® Safe, or comparable blue

light-compatible, DNA stain was added to the gel.

Consider a post-electrophoresis stain like EDVOTEK®

FlashBlue™ stain.

• Verify that the gel has run properly. This can often be

conrmedbythemigrationofaloadingdyeincluded

in the experimental samples.

The gel has run crooked, or the DNA samples are at an

angle:

• Ensure that the gel tray is aligned properly in the

buerchamber.Thetrayshouldsitushagainstthe

bottom of the chamber and against one wall of the

chamber to ensure it is straight.

• Check that the electrodes are intact and that there is

no visible corrosion on either electrode.

Power Switch

Fuse

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