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OPERATION MANUAL EDGE™Integrated Electrophoresis System
4
Specifications:
• LED Wavelength: 470 nm +/- 10 nM
• LED Life: 50,000 hours
• Input voltage: 100-240V AC, 50/60 hz
• Output voltage: Selectable at 100V or 150V DC
• Dimensions: 17 cm (w) x 17 cm (l) x 15 cm (h)
• Operating conditions: 4 °C to 37 °C,
75% maximum humidity
Guarantee:
The unit is guaranteed against any defect in material or
workmanship for three years. The warranty period is from
the date of receipt, and within this period all defective
parts will be replaced free of charge provided that the
defect is not the result of misuse, accident or negligence.
Servicing under this guarantee should be obtained from
EDVOTEK®.
Notwithstanding,thedescriptionandspecication(s)ofthe
units contained in the User’s Manual, EDVOTEK® hereby
reservestherighttomakesuchchangesasitseesttothe
units or to any component of the units. This Manual has
been prepared solely for the convenience of EDVOTEK®
customers and nothing in this Instruction Book shall be
taken as a warranty, condition or representation concern-
ingthedescription,merchantability,tnessforpurposeor
otherwise of the units or components.
Contact Information:
For technical, sales or servicing information, contact:
EDVOTEK, Inc.,
1121 5th Street NW
Washington, DC 20001
1.800.EDVOTEK (1.800.338.6835)
Fax: 202.370.1501
Troubleshooting:
The EDGE™ does not power on:
• Check that the power cord is fully plugged into the
EDGE™ base and that the cord and outlet are func-
tional.
• Conrmthatthepowerswitchontherearofthe
EDGE™ base is turned on.
• Inspect the fuse and replace if needed. The fuse is
located directly under the power plug on the rear of
the EDGE.
The START/STOP indicator LED does not stay lit and/or
the experiment does not run:
• Ensure that the lid is fully closed.
• Pressrmlyontheelectrophoresistanktoensure
thatitisrmlyseatedandthatbothelectrodesare
making contact with the base.
• Checkthatthecorrectbuerhasbeenusedandthat
thegelisfullysubmergedinbuer.
• Ensure that the timer has not reached zero. Increase
the remaining time and try running the experiment
again.
The gel tray will not t into the chamber:
• Thetrayisdesignedtotonlyoneway.Rotatethe
tray180˚andtryagain.
• TheEDGE™usescustom10x7cmtraysthatdier
from existing Edvotek electrophoresis accessories.
Please ensure that you are using the proper electro-
phoresis trays.
No DNA bands are present on the gel:
• Check that the blue light is turned on by pressing the
paddle switch
• Ensure that SYBR® Safe, or comparable blue
light-compatible, DNA stain was added to the gel.
Consider a post-electrophoresis stain like EDVOTEK®
FlashBlue™ stain.
• Verify that the gel has run properly. This can often be
conrmedbythemigrationofaloadingdyeincluded
in the experimental samples.
The gel has run crooked, or the DNA samples are at an
angle:
• Ensure that the gel tray is aligned properly in the
buerchamber.Thetrayshouldsitushagainstthe
bottom of the chamber and against one wall of the
chamber to ensure it is straight.
• Check that the electrodes are intact and that there is
no visible corrosion on either electrode.
Power Switch
Fuse