DINKO Instruments UV/VIS 6000 Manuel utilisateur
DINKO
INSTRUMENTS
User’s Manual UV/VIS 6000
1
UV/VIS 6000 Ultraviolet Visible Spectrophotometer
Smart Touch Screen
Reference Manual
Version: March 2021
Mark
c/ Encarnació, 123-125 / 08024-Barcelona
dinter@dinko.es www.dinko.es
DINKO
INSTRUMENTS
User’s Manual UV/VIS 6000
2
Dear user:
Thank you for purchasing and using the instrument of shanghai metash
instrument corporation. Please read the manual carefully to enable you to
know the performance of the instrument as soon as possible and to master
its using method.
The copyright of the manual belongs to shanghai metash instrument
corporation. Without the permission of our company, deleting and copying
of the contents in any form is forbad for anybody.
It is possible to modify the contents of the manual for the need of improving.
If any modification happens, please forgive us no further notice.
You can log on to our official website http://www.dinko.es for updates, or
call our hotline +34 93 284 69 62, to obtain the relevant advice and help.
Although we have checked this manual carefully, some mistakes cannot be
avoided, if any inconvenience is caused, please understand. If you find
some mistakes or have better suggestions, you are welcome to get in touch
with us to enhance our further improvement. we will actively adopt you
rational suggestions to ensure that our instrument will be improved
continually to meet your needs.
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INSTRUMENTS
User’s Manual UV/VIS 6000
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Contents
1. Summary............................................................................................5
1.1 Working Principle .....................................................................5
1.2 Instrument Characteristics.........................................................6
1.3 Application Field.......................................................................7
1.4 Technical Parameters.................................................................8
1.5 Symbols and Prompt Notes .......................................................8
1.6 Brief Introduction of System and Structure ...............................9
2. Installation .......................................................................................10
2.1 Unpacking...............................................................................10
2.2 Installation Condition..............................................................10
2.3 Instrument Installation.............................................................12
3. Operating the Instrument..................................................................12
3.1 Power on Self-test ...................................................................12
3.2 Photometric Analysis...............................................................16
3.2.1 Photometric analysis.......................................................17
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User’s Manual UV/VIS 6000
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3.2.2 Data processing ..............................................................18
3.3 Quantitative Analysis ..............................................................20
3.3.1 Standard curve test .........................................................21
3.3.2 Application of coefficient method .................................................29
3.3.3 Setting concentration unit..............................................................31
3.4 Spectral Scanning.......................................................................32
3.4.1 Wavelength scan................................................................34
3.4.2 Processing data................................................................374
3.5 Kinetic Analysis.......................................................................429
3.5.1 Kinetic analysis.................................................................41
3.5.2 Processing data................................................................462
3.6 System Settings........................................................................517
3.6.1 Lamps management............................................................ 48
3.6.2 Time Settings.....................................................................50
3.6.3 Dark current calibration...................................................561
3.6.4 Wavelength calibration....................................................572
3.6.5 System debugs.................................................................583
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3.6.6 Restoring defaults............................................................584
4. Routine maintenance........................................................................606
5. Troubleshooting ...............................................................................629
1.Summary
1.1 Working Principle
The working principle of spectrophotometer is mainly based on Lambert -
Bill law. When a bundle of monochromatic light passes through a uniform
colored solution, the absorbance of the solution is proportional to the
product of the concentration of the solution and the optical path,
which is
the true physical meaning of Lambert Bill's law. The law is the basis of
quantitative analysis of photometric analysis, and also the basic principle
of UV-Vis spectrophotometer. The formula for Lambert-Bill law is
expressed as follows:
A=k b C
A--The absorbance of the measured substance solution.
k-- Absorption coefficient of solution.
b--
The thickness of the liquid layer (the optical path of the cuvette or
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User’s Manual UV/VIS 6000
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absorption pool), cm.
C—The concentration of the measured substance solution.
The premise of satisfying the Lambert Bill law is three hypotheses: First, the
incident light is monochromatic light. Second, the incident light is parallel light.
Third, light-absorbing particles are independent of each other, regardless of
quantity and variety. In addition, the deviation of the law is affected by sample
treatment, the optical path of measure, stray light, noise, the spectral bandwidth,
the chemical factors and other factors. The abovefactors need be taken into account
in the routine tests.
1.2 Instrument Characteristics
The characteristics of UV/VIS 6000 Spectrophotometer are the following:
The stray light is low and resolution is high in optical system, so the
stability and reproducibility are excellent and the readings are accurate.
The design is scientific, new technologies are used to combine the light,
instrument, electricity with computer to make the stability indicators of
the instrument close to or reach the high-level of UVspectrophotometer.
7 inch TFT capacitive touch screen, the touch point is more accurate,
sensitivity is more higher and stability is more better.
High definition resolution (800 * 480), fast running, large capacity for
picture.
Interactive and friendly user interface, and it is easy and fast to be used.
System settings function is powerful, the measurement function of
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photometric analysis, quantitative analysis, wavelength scan and
kinetic analysis can be realized without online operation.
Large data storage: 30 wavelength scan files, 20 kinetic measurement
files, multiple sets of measurement data and standard curves.
Data retrieval and atlas zooming function.
The universal printer (inkjet or laser) can be connected with the
instrument, A4 report output can be completed directly and the report
is more clear and beautiful.
USB transfer function can be achieved; data storage and reading is
more flexible.
1.3 Application Field
Ultraviolet visible spectrophotometer can be widely used in the fields of
pharmacy, medical and health services, chemical engineering, energy,
machinery, metallurgy, environmental protection, geology, food, biology,
materials, agriculture, forestry, fisheries and other industries, and plays an
important role in teaching and research of higher education, measurement
science and research institutes, quality control in the production, raw
materials and product analysis, and is commonly used in physicochemical
laboratory. UV/VIS 6000 is a UV spectrophotometer with touch screen.
The instrument is stable, accurate and powerful and has obvious
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advantages in the fields of scientific research and quality control.
1.4 Technical Parameters
Type UV/VIS 6
Wavelength range (nm) 19 -11
Spectral bandwidth (nm) 1,8 nm
Wavelength accuracy (nm) ± .5
Wavelength repeatability
(nm) ≤ .2
Photometric range -2 %T、- .3-3A、-9999C
Photometric accuracy ± .4 %T
Stray light ≤ . 7 %T
Size (mm) 46 ×38 ×18
1.5 Symbols and Prompt Notes
:
Power supply,
beware of electric shock.
: Heat source, burn carefully.
: UV light source, be careful radiation.
: Notice.
: Special note.
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1.6 Brief Introduction of System and Structure
1、The shape and structure of the instrument
The shape and structure of the instrument is shown in figure 1:
1 2 3
1--Hand pull rod 2—Sample room 3--Touch screen
Figure 1 The shape of UV/VIS 6000
The back of the UV/VIS 6000 instrument is shown in figure 2:
1—Power switch 2-- Power supply socket 3-- Cooling vent
4-- Printer port
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User’s Manual UV/VIS 6000
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Figure 2 The back of UV/VIS 6000
2.Installation
Please read the contents of this chapter carefully before installation.
2.1 Unpacking
Please check to make sure that the instrument external packing is intact and
has no opened marks before unpacking. After unpacking, please inspect to
make sure the instrument has no damage and the accessories is complete
by referring to the packing list in the manual (page thirty-fifth of this
manual). If you have any question, please consult the dealer or
manufacturer. If you find any damage or loss in the package, please inform
the manufacturer immediately.
2.2 Installation Condition
The following requirements should be met for the installation of the
instrument:
1) The requirements of temperature and humidity:
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