Diffinity Genomics RapidTip Mode d’emploi
Difnity Genomics
RapidTipTM User Handbook
Version D (December 2010)
Table of Contents
Kit Contents 3
storage 3
ProduCt use Limitations 4
ProduCt Warranty and satisfaCtion guarantee 4
QuaLity ControL 5
teChniCaL assistanCe 5
safety information (disPosaL) 5
ProduCt sPeCifiCations 6
introduCtion 7
diffinity teChnoLogy PrinCiPLe 8
eQuiPment to be suPPLied by user 9
PiPetting With the raPidtiP 10
raPidtiP PCr PurifiCation ProtoCoLs 11
troubLeshooting guide 13
ordering information 14
Page 2 www.difnitygenomics.com
Kit Contents
Cat Number Product
Description
Contents
RT025-008 Difnity RapidTip for
PCR Purication
1 Pack of 8
RapidTips
RT025-048 Difnity RapidTip for
PCR Purication
1 Box of 48
RapidTips
RT025-096 Difnity RapidTip for
PCR Purication
1 Box of 96
RapidTips
RT025-096 * Difnity RapidTip for
PCR Purication
* Multiple box
purchases (3,
12, 24, and 48)
are also available
All Kits come with a User Manual
Storage
Difnity RapidTips should be stored in low humidity
at room temperature (15-25 degrees Celsius). Under
these conditions, Difnity RapidTips can be stored
and used for up to 12 months without reduction in
performance or quality.
Difnity RapidTip Page 3
Product Use Limitations
Difnity RapidTips are intended for research use
only. Difnity RapidTip is designed for purication
of PCR solutions prior to dideoxy (BigDye) reaction
for Sanger Sequencing. RapidTip is compatible with
most universal pipettors. RapidTip has not been
tested for compatibility with detergents, mineral
oil, nor ready-to-load style PCR master mixes that
contain a density increasing compound.
Product Warranty and Satisfaction
Guarantee
Difnity Genomics will guarantee the performance
of Difnity RapidTips for the uses and performance
as described in our product literature. If the product
fails to perform to your satisfaction for any reason
other than misuse, we will replace the product free
of charge or refund the purchase price - whichever
you choose. Difnity Genomics maintains the right
to update or modify the product to enhance its
performance. A copy of our terms and conditions
can be found on our web site.
If you have questions about the product or its
performance, please contact Difnity Genomics
customer service at 1-877-362-1812.
Page 4 www.difnitygenomics.com
Quality Control
Each lot of Difnity RapidTips is tested against
predetermined specications to ensure consistent
quality.
Technical Assistance
Call 1-877-362-1812 between 8am and 4pm Eastern
time Monday through Friday or visit our webpage
for help with common questions.
Safety Information (Disposal)
Difnity RapidTips may be disposed in regular lab
trash. For more information, please consult the
material safety data sheet (MSDS).
Difnity RapidTip Page 5
Product Specications
Capacity: PCR Sample
Size
25 +/- 5 microliters
dsDNA Recovery up to 90%
(100bp to 10Kb)
Impurity Removal
(dNTP, primers, primer-
dimers)
up to 90%
(less than 45-50 bp)
Please note that at this time the Difnity RapidTip
does not remove DNA polymerase. Standard DNA
polymerase such as Taq does not typically interfere with
the sequencing amplication (BigDye) reaction.
Page 6 www.difnitygenomics.com
Introduction
Difnity Genomics has developed an innovative new
technology that removes undesirable impurities
from PCR reactions leaving you with nothing
but puried DNA! This revolutionary technology
effectively removes dNTPs, primers, and primer-
dimers while providing up to 90% recovery of pure
DNA fragments ranging in length from 100bp to
10Kb. It dramatically reduces the amount of time
to purify and prepare PCR reactions for use in
downstream applications, such as DNA sequencing.
The RapidTip is one-tip, one-step, and one-minute
for PCR purication.
Difnity RapidTip Page 7
Difnity Technology Principle
The Difnity RapidTip contains everything you
need for PCR purication. The tip is lled with our
proprietary adsorption technology, which has a
differential afnity for PCR reaction components.
The impurities are removed from the solution as
it enters the pipette tip. Dispensing the solution
yields puried, high quality DNA ready to use for
downstream applications. You won’t need additional
equipment, reagents, buffers, or enzymes - only a
pipettor!
.5Kb 1Kb 2Kb
L- + - + - + L
Difnity RapidTip Purication has excellent yield. Equal volumes
of untreated (-) and Difnity RapidTip treated (+) PCR product
(.5Kb, 1Kb, 2Kb) were run on a 1% agarose gel alongside
Invitrogen 1Kb Plus Ladder (L) and labeled with SybrSafe.
Page 8 www.difnitygenomics.com
unpuried
Difnity RapidTip
Difnity RapidTip purication improves sequence quality.
Sequence chromatograms show improved peak quality and lower
background in Difnity RapidTip puried samples as compared to
unpuried samples.
Equipment to be Supplied by User
• PCR Sample
• Tubes to store puried DNA
• Pipettor - manual or programmable
• Standard pipette tips for liquid transfer
(if necessary)
Difnity RapidTip Page 9
Pipetting with the RapidTip
Careful pipetting is important to achieve effective
purication with the RapidTip. The following
pipetting guidelines will help ensure optimal results:
1. Aspirate slowly to ensure particles are dispersed
for effective mixing.
If using a manual pipettor, aspirate slowly for the
rst several cycles until the particles are completely
dispersed into the PCR sample.
If using an automated pipettor, set to aspirate on
low speed (2 of 10 or equivalent), dispense on
middle speed (5 of 10 or equivalent) and place into
manual mix mode.
2. Maintain continuous contact between the
end of the pipette tip and your sample. Avoid
disrupting the uid column with air bubbles.
3. Maintain clearance between the end of the
pipette tip and the plastic tube or well holding
sample to avoid plugging the tip during sample
mixing.
Page 10 www.difnitygenomics.com
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