Coyote Mini8 Plus Manuel utilisateur

Coyote Bioscience Co., Ltd.

Tel: 010-64844237

Fax: 010-64844237-8000

Add: Room A211/213, 2/F, Shangdixinxilu#12 Haidian District, Beijing, China. 100085

www.coyotebio.com

Mini8 Plus

Real-Time PCR System

Coyote, highly specialized in research & development, offers

integrate molecular diagnostic solutions by providing devices

from sample pre to gene amplification / detection.

Version：EN / 20160413

Overview

Introduction

Setup

Unpack the Mini8 Plus System

Place Mini8 Plus on the Bench

Connect Mini8 Plus

Install the Mini8 Plus Software

Turn on the Mini8 Plus System

Workow

Mini8 Plus System Workow

Load the reaction tubes

Dene a New Experiment

Set Up the Thermal Prole

Dene the Plate Layout

Monitor Run

Data Analysis

System Information

Lights

Specications and Environmental Requirements

Electromagnetic Compatibility

Cleaning and Maintenance

Concepts

Chapter 1

Chapter 2

Chapter 3

Chapter 4

Chapter 5

Contents

Introduction

Coyote Mini8 Plus Real-Time PCR System is designed for molecular

diagnostic market.

It has a small footprint, with high detection sensitivity. The machine has

two channels, one is SYBR/FAM, and the other is Texas Red / ROX. The

sample capacity is 8 × 0.2mL PCR tubes, and it is compatible with any

commercial SYBR®or TaqMan®qPCR kits.

The user interface on the computer is friendly and very simple to operate.

The uorescence dye preference can be customized. This product

requires 12V DC power supply, that is compatible with a car charger or a

battery pack.

Coyote Bio engaged on designing and manufacturing innovative

personal devices for both life science and molecular diagnosis. All our

devices were vested portable features for eld applications with 12V DC

power or batteries while all redundant features were minimized and the

key functions were developed more powerful and user-friendly. Therefore

Coyote’s devices are easy to handle and set, means that the labor cost

could be signicantly reduced.

Service: Coyote Bioscience Co., Ltd.

Registered Address: 509, 510, 5th oor, Chuangyezhonglu#36, Haidian,

Beijing

ZIP: 100085

TEL: 010-64844237

FAX: 010-64844237-8000

SALES: [email protected]

Overview

Unpack the Mini8 Plus System

1. Lift the Mini8 Plus instrument out of the package. Place it on a

at surface and remove the packaging materials (Keep the box and

packaging in case of a return).

2. Check the packing list as follows to ensure that all components are

present and intact. Your system comes with:

A. Mini8 Plus instrument

B. Power adapter

C. Power cable

D. USB cable

E. DVD

F. User's manual

Place Mini8 Plus on the Bench

Benet from its mini size, two distances of only 10cm (4 inches) for each

sides of left and right are enough for ventilation, and also a distance of

5cm (2 inches) above is necessary for opening the lid.

Connect Mini8 Plus

1. Connect one connector of the USB cable to the port on the computer.

Connect the other connector to the port on the side panel of the Mini8

Plus.

2. Connect the Mini8 Plus power cable to the DC power inlet on the side

panel, and then to the wall outlet.

3. Plug the computer power cable to the wall outlet.

Setup

A B C D E F

Mini-8

Real-Time PCR System

Install DVD

Version 14.0.3

Coyote Biotech Company

www.coyotebiotech.com

[email protected]

Install Mini8 Plus software

·Connect Mini8 Plus to computer and switch on the device;

·Load CD or open the software le, click setup program.

1) Before installation the Prerequisites Wizard must be started running to

set up the environment as follow:

Click Next

PC port

Mini8 Plus port

A B C

Power cable

USB cable Power adapter

ABC

Select ‘DriverInstaller Application’, ‘.NET Framework 4.0’, ‘NI LabVIEW

Runtime 2014 SP1 f3’ and Click next. Click‘next’ per dialog box

continuously to install ‘DriverInstaller Application’, ‘.NET Framework 4.0’,

‘NI LabVIEW Runtime 2014 SP1 f3’.

Click Install

Click OK

Click Next

4 5

Click Install

Click Finish

·The System will automatically detect the operating environment, and

check the installation options, If a project has been installed, you can

manually cancel.

·Click next to start the installation.

Note: During the installation, the system may be requested to restart, you

can choose to restart later.

Click Next

6 7

Select installation

path and click

Next to continue

Click Next

2) The software installation will be boot up once the environment

detection completed.

Click Install

to start the

installation

The Mini8 Plus

software is

installing

8 9

After the

installation

click Finish to

close the setup

program

Turn on the Mini8 Plus System

Turn on the Mini8 Plus instrument, then double-click the Mini8 Plus icon

to start the software.

*When the Power indicator lights on the front panel stop ashing and

remain solid, the instrument is ready.

Mini8 Plus System Workow

1. Prepare the sample strip, load it into the Mini8 Plus, and close the lid.

2. Double-click the Mini8 Plus icon on desktop to open the

software.

3. Dene and name the experiment, save the experiment.

Tip: to use a pre-dened thermal prole and plate layout for your

experiment, click and select one of the template experiments

saved in your computer.

4. Review the thermal prole and adapt it if needed.

5. Set up the plate layout by dening assays, samples, and standards and

assigning them to wells.

6. Start the run. The Monitor Run tab opens.(Do not open the lid while a

run is in progress.This will corrupt the data.)

7. When the run is completed, open the Mini8 Plus lid. Remove the strip

from the block. Dispose of any hazardous materials into appropricate

containers for biohazard, caustic materials, according to your local safety

regulations.

Load reaction tubes

1. Thaw all necessary reagents (templates, primers, probes, and master

mix).

2. Turn on the computer, then the Mini8 Plus, and wait until the Mini8 Plus

"Power" light is ashing (Standby mode)

3. Conrm that the block and optical path are clear of visible

contaminants and there is no physical damage to the system.

4. Pipette samples and qPCR reagents into the strip according to your

protocol. (Warning: Wear protective gloves and eyewear when operating

with any material that might be considered caustic or hazardous.)

5. Open the Mini8 Plus lid and place the strip on the dock.

6. Close the Mini8 Plus lid.

7. Proceed to dene a new experiment.

Workow

10 11

1. Double-click the Mini8 Plus icon on the desktop to open the software.

Advanced user options (optional functions)

2. Click "New Experiment", the New Experiment tab opens, enter an

experiment name and sample type.

Dene a New

Experiment

Select advanced

user options and

click OK

Warning

Do not touch hot lid. The hot lid temperature would be up to 105°C

(221°F) when the device is working .

2) Fill in your Email, when the running is over, the result report (Excel le)

will be sent to your email.

PS. The computer need to be connected to the network.

1) If you want to pause running when ∆Rn reaches the setting value,

please input terminal ∆Rn for each sample. This function can help you to

get the PCR amplication product in the status you want.

12 13

1. Click , the Setup window opens, with the Thermal Protocol

tab visible.

2. Click / to add/delete segment.

3. Click / to add/delete step.

4. The camera icon indicates that the uorescence is being read.

5. Click to add the Melt Curve prole.

6. Click to save Protocol

7. Click to choose and use the saved Protocol (*.pdt)

Set up the Thermal Prole

The "plate setup" involves the following steps:

1. Click , the Plate Setup window opens.

2. Click to choose the sample.

3. Set up sample name.

4. Set up sample type: unknown, positive control, negative control, standard.

When the sample type is dened as “Standard”

the concentration setting interface will be promoted for user to set the

“Standard” concentration in the following interfaces:

Dene the Plate Layout

Dene Sample Name

Dene Sample Type

Dene Fluorophore CH1(None/SYBR/FAM)

Dene Fluorophore CH2(None/Texas Red/ROX)

Dene the concentration unit for samples

Click to choose the sample(1-8)

14 15

5. Set up uorophore CH1(None/SYBR/FAM)

6. Set up uorophore CH2(None/Texas Red/ROX)*

7. Click to save plate layout

8. Click to choose and use the saved plate

Click to enter the running interface, and click to

start the running.

Click to pause the running.

Click to stop the running.

Monitor Run

Cycle Numbers(You can increase and

reduce the cycle numbers during the cycling)

Data Collection Point

Melt Curve

Drag Bar Up or Down to Adjust

Temperature and Duration/

Click Temperature Plateau to Adjust

Temperature and Duration

Remaining Time

Warning

Do not open the lid while a run is in progress. It may allow extraneous

light enter the system so the data will be corrupted.

Note

If you do not set up the uorophore channel when set the thermal prole,

there will be not any curve showing neither during nor after the process

of amplication, for the uorescence will not be read.

When the running is over, click to save data(*.edt).

The Real time PCR curve will be showed during the amplication.

Channel switch

16 17

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