Quick Start
MULTISPLIT V2
Quick start guide July 2018
The MultiSplit is calibrated and QC’d prior to delivery, however some alignment is required upon initial
setup using the calibration cubes provided (containing a 50% mirror). A detailed setup guide is also
supplied; however, the following simplified instructions are useful for initial alignment.
2. Using brightfield illumination, focus on an image of a graticule and divert the microscope output to the camera.
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1. Ensure all filter cubes are removed from the unit.
3. Close the adjustable aperture down to ensure one, small rectangle is present within the field of view. This single image is
only reflected by the internal MultiSplit mirrors and is therefore by default the longest wavelength channel once filter cubes are
in place. This image is transmitted through two dichroic mirrors and referred to as TT.
Image TT
No cubes in the unit
4. Locate the horizontal and vertical image separation controls at the front of the unit (labelled on the lid). When turned
anti-clockwise, TT should move toward the bottom right corner. If this is not the case, rotate your camera until this orientation
is achieved.
TT
