Biotix xPipette Manuel utilisateur
2
xPIPETTE™
Table of Contents Introduction
Introduction
Parts Checklist ........................................ 3
GLP Compliance ..................................... 3
Description .............................................. 3
Specications .......................................... 4
Setting Volume
Instructions .............................................. 6
Pipetting
Tip Selection ............................................ 8
Tip Mounting ............................................ 8
Operation ................................................. 8
Dispensing ............................................... 8
Good Pipetting Guidelines ....................... 9
Pre-rinsing ............................................... 9
Pipetting Liquids of Varying Densities ...... 9
Temperature Considerations ...................... 9
Reverse Pipetting .................................. 10
Troubleshooting
Troubleshooting and Repairs ................. 11
Sample Splash ...................................... 12
Leaks, Inaccuracy, Abnormal Stroke ..... 12
Service, Calibration and Repair ............. 12
Maintenance
Autoclaving ............................................ 13
Tip Ejector Arm Removal ....................... 13
Storage .................................................. 13
Acids and Corrosives ............................. 13
Parts ....................................................... 13
Accessories
Pipette Carousel .................................... 14
Replacement Parts ................................ 14
Warranty
3
6
8
11
13
14
14
3
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Table of Contents Introduction
3
6
8
11
13
14
14
xPIPETTE is an air-displacement pipette that incorporates
major ergonomic improvements to minimize strain and
help reduce the risk of repetitive stress injuries. Low spring
forces and low force seals contribute to a lighter feel. A
magnet is used to help balance and hold the piston in the
zero position, which reduces static force on the operator’s
hand during the pipetting cycle.
Parts Checklist
After unpackaging, please verify that the following items
were included and are undamaged:
• xPIPETTE
• xPIPETTE User Guide
• Calibration Certicate
GLP Compliance
The unique pipette serial number is
engraved on the body of the pipette
underneath the handle. It provides
a unique identication to to help
fulll GLP requirements.
Calibration certicates are also
included for further traceability.
Description
Connecting Nut
Shaft
Stainless Steel
Tip Ejector Arm
Digital Volume Indicator
Plunger
Body:
high-impact, low thermal
conductivity polymer
Handle
Volume Lock
Tip Ejector
2 μL
1
2
5
1.25 μL
10 μL
0
7
5
7.5 μL
20 μL
1
2
5
12.5 μL
100 μL
0
7
5
75 μL
200 μL
1
2
5
125 μL
300 μL
2
2
5
225 μL
1000 μL
0
7
5
0.75 mL
0
6
7
1
0
8
1
5
67 μL 108 μL 157.6 μL
xPIPETTE Manual
4
xPIPETTE™
Specications
Single Channel Pipettes
BIOTIX ISO 8655
Volume* Systematic
Error
Random
Error
Systematic
Error
Random
Error
2 μL with xTIP4 20 μL tips (P/N 63300160)
0.2 ± 0.024 ≤ 0.012 ± 0.08 ≤ 0.04
1± 0.027 ≤ 0.013 ± 0.08 ≤ 0.04
2± 0.03 ≤ 0.014 ± 0.08 ≤ 0.04
10 μL with xTIP4 20 μL tips (P/N 63300161)
1± 0.025 ≤ 0.012 ± 0.12 ≤ 0.08
5 ± 0.075 ≤ 0.03 ± 0.12 ≤ 0.08
10 ± 0.1 ≤ 0.04 ± 0.12 ≤ 0.05
20 μL with xTIP4 20 μL tips (P/N 63300162)
2± 0.15 ≤ 0.04 ± 0.20 ≤ 0.10
10 ± 0.15 ≤ 0.05 ± 0.20 ≤ 0.10
20 ± 0.2 ≤ 0.06 ± 0.20 ≤ 0.10
100 μL with xTIP4 200 μL tips (P/N 63300163)
10 ± 0.35 ≤ 0.10 ± 0.80 ≤ 0.30
50 ± 0.4 ≤ 0.12 ± 0.80 ≤ 0.30
100 ± 0.8 ≤ 0.15 ± 0.80 ≤ 0.30
200 μL with xTIP4 200 μL tips (P/N 63300164)
20 ± 0.5 ≤ 0.20 ± 1.60 ≤ 0.60
100 ± 0.8 ≤ 0.25 ± 1.60 ≤ 0.60
200 ± 1.6 ≤ 0.30 ± 1.60 ≤ 0.60
300μL with xTIP4 300 μL tips (P/N 63300327)
30 ± 0.75 ≤ 0.30 ± 2.40 ≤ 0.90
150 ± 1.2 ≤ 0.375 ± 2.40 ≤ 0.90
300 ± 2.4 ≤ 0.45 ± 2.40 ≤ 0.90
1000μL with xTIP4 1000 μL tips (P/N 63300165)
100 ± 3 ≤ 0.6 ± 8.0 ≤ 3.0
500 ± 4 ≤ 1 ± 8.0 ≤ 3.0
1000 ± 8 ≤ 1.5 ± 8.0 ≤ 3.0
These manufacturer’s specications should be used as
guidelines when establishing your own performance
specication.
5
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Specications
Multi-Channel Pipettes
BIOTIX ISO 8655
Volume* Systematic
Error
Random
Error
Systematic
Error
Random
Error
10 μL with xTIP4 20 μL tips
(P/N 63305170 & 63305174)
1± 0.040 ≤ 0.05 ± 0.08 ≤ 0.10
5 ± 0.075 ≤ 0.075 ± 0.15 ≤ 0.15
10 ± 0.100 ≤ 0.05 ± 0.20 ≤ 0.10
20 μL with xTIP4 20 μL tips
(P/N 63305171 & 63305175)
1 ± 0.15 ≤ 0.08 ± 0.30 ≤ 0.16
5± 0.15 ≤ 0.15 ± 0.30 ≤ 0.30
10 ± 0.20 ≤ 0.10 ± 0.40 ≤ 0.20
200 μL with xTIP4 200 μL tips
(P/N 63305172 & 63305176)
2 ± 0.50 ≤ 0.24 ± 1.00 ≤ 0.48
10 ± 0.80 ≤ 0.25 ± 1.60 ≤ 0.50
20 ± 1.60 ≤ 0.60 ± 3.20 ≤ 1.20
300 μL with xTIP4 300 μL tips
(P/N 63305173 & 63305177)
10 ± 0.75 ≤ 0.10 ± 1.50 ≤ 0.20
50 ± 1.20 ≤ 0.12 ± 2.40 ≤ 0.30
100 ± 2.40 ≤ 0.15 ± 4.80 ≤ 0.30
*Specications are subject to change without notice
* All volumes are in microliters
xPIPETTE Manual
6
xPIPETTE™
Setting the Volume
Unlocked Locked
Volume is locked when
wings are straight across
Wings
Instructions
1. Turn the volume lock counter-clockwise to the position
shown at left below so the volume setting mechanism is
unlocked and free to turn.
2. With the mechanism unlocked, orient the pipette so you
are looking at the digital volume indicator, then rotate the
plunger button to change volume – counter-clockwise to
increase, clockwise to decrease volume.
The volume indicator is read from the top down and is
color-coded in relation to where the decimal point should
go.
2-20 μL: Black - μL.
Red- tenths, hundredths of μL.
100 -300 μL: All digits black - whole μL.
1000 μL: Red - mL. Black - tenths, hundreds of mL.
3. To eliminate errors due to mechanical backlash: when
setting the desired volume, rst turn the knob 1⁄3 turn
above the desired volume. Then turn the knob slowly
clockwise until the desired volume is displayed.
4. Turn the volume lock clockwise (see diagram above) to
prevent any accidental changes to the volume setting.
Example volumes are shown on the next page (note the
intermediate setting at the right).
2 μL
1
2
5
1.25 μL
10 μL
0
7
5
7.5 μL
20 μL
1
2
5
12.5 μL
100 μL
0
7
5
75 μL
200 μL
1
2
5
125 μL
300 μL
2
2
5
225 μL
1000 μL
0
7
5
0.75 mL
0
6
7
1
0
8
1
5
67 μL 108 μL 157.6 μL
2 μL
1
2
5
1.25 μL
10 μL
0
7
5
7.5 μL
20 μL
1
2
5
12.5 μL
100 μL
0
7
5
75 μL
200 μL
1
2
5
125 μL
300 μL
2
2
5
225 μL
1000 μL
0
7
5
0.75 mL
0
6
7
1
0
8
1
5
67 μL 108 μL 157.6 μL
7
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Setting the Volume
Volume ranges and increments for single-channel pipettes
are shown below:
Volume ranges and increments for multi-channel pipettes are
shown below:
Pipette Size
(μL)
Adjustable
Range
(μL)
Recommended
Volume Range
(μL)
Increment
(μL)
2 0 - 2 0.1 - 2 0.002
10 0 - 10 0.5 - 10 0.02
20 0 - 20 2 - 20 0.02
100 0 - 100 10 - 100 0.2
200 0- 200 20 - 200 0.2
300 0 - 300 30 - 300 0.5
1000 0 - 1000 100 - 1000 2.0
Pipette Size
(μL)
Adjustable
Range
(μL)
Recommended
Volume Range
(μL)
Increment
(μL)
10 0 - 10 0.5 - 10 0.02
20 0 - 20 2 - 20 0.02
200 0- 200 20 - 200 0.02
300 0 - 300 30 - 300 0.5
xPIPETTE Manual
8
xPIPETTE™
Pipetting
Tip Selection
To ensure top performance of your xPIPETTE, we recom-
mend that you use Biotix xTIP4 pipette tips. Together, the
pipette-tip combination creates an ergonomic system to
reduce insertion and ejection forces. The xPIPETTE is also
fully compatible with Rainin®LTS™tips.
Tip Mounting
Single-Channel Pipette: To mount a tip, press the pipette
shaft nozzle into the end of the tip with light force.
Multi-Channel Pipette: Align the shaft nozzles into the row
of tips, holding the pipette at an angle. Position the pipette
upright and press the nozzles into the tips until securely
mounted.
Operation
Before pipetting valuable samples, we recommend that you
practice aspirating and dispensing water before pipetting
with actual samples.
1. Set the desired volume as described on page 7.
2. Attach a new tip. Press the shaft into the tip to make a
good seal.
3. Press the plunger button to the FIRST STOP, and hold it in
this position. The magnetic assist will help you sense and
hold this position.
4. Holding the pipette vertically, place the tip into the sam-
ple to the proper depth and relax your thumb pressure
on the plunger. The light piston spring will move the
piston upward, aspirating your sample. Do not let go of
the plunger button suddenly, or the piston may snap up
quickly, resulting in an inaccurate measurement.
5. Pause briey to ensure that the full volume of the sample
is drawn into the tip.
6. Withdraw the tip from the sample. If any liquid remains
on the outside of the tip, wipe it carefully with a lint-free
tissue, taking care not to touch the tip orice.
Dispensing:
1. Touch the tip end against the sidewall of the receiving
vessel and press the plunger slowly, past the rst stop, to
blowout (bottom of stroke).
First Stop
Blowout
Rest Position
Step 1 Step 2
9
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Pipetting
Wait: 1-2 seconds for 2–300 μL volumes, 2-3 seconds for
1000 μL (longer for viscous solutions).
2. Still holding the plunger, withdraw the tip, sliding it along
the wall of the vessel. Release the plunger.
3. Press the tip ejector button to discard the tip. Use a new
tip for each sample to prevent carry-over. Repeat for the
next pipetting cycle.
Good Pipetting Guidelines
xPIPETTE incorporates features to deliver pipetting
consistency. You should also maintain:
1. Consistent pickup and dispense rhythm.
2. Consistent speed and smoothness when pipetting.
3. Consistent pressure on the plunger button at the rst
stop.
4. Consistent immersion depth.
5. Pipette vertically, or within 20° of vertical.
6. Don’t invert or lay the pipette at with liquid in the tip.
Pre-Rinsing Recommended
Some solutions may leave a lm on the inside tip wall. This
lm remains relatively constant in successive pipetting with
the same tip, so excellent precision can be obtained by
relling the tip and using the relled volume as the sample.
Successive samples from this same tip will exhibit good
reproducibility.
Temperature Considerations
Warm or cold liquids can be measured with good preci-
sion by using a consistent pipetting rhythm. This will help
minimize any dierences in heating or cooling eects within
the pipette. Use a new pipette tip each time for optimal
accuracy and precision when measuring samples with
temperatures greatly dierent from ambient, and do not
pre-rinse. As with any air-displacement pipette, you will get
the best results if there is no delay between aspirating the
sample and dispensing it.
Volume Range (μL) Immersion Depth
(mm)
Wait Time
(sec)
0.1-10 1-2 1
10-200 2-3 1
200-1000 3-6 2-3
xPIPETTE Manual
1 0
xPIPETTE™
Pipetting Liquids of Varying Density
xPIPETTE adjustable pipettes allow you to compensate for
solutions with densities that are dierent than water, by
setting the volume slightly higher or lower. The compensa-
tion amount must be determined empirically. For example,
if pipetting 10 μL of CsCl solution, you determine that the
volume delivered is actually 8.5 μL. Change the volume set-
ting to 11.8 μL and repeat the measurements. If the volumes
delivered are still not close enough to 10 μL, make another
slight volume adjustment until the measurements are as
desired.
Reverse Pipetting
Another technique for reducing error due to lm retention,
especially
useful for more viscous liquids, is reverse pipetting. The
operating sequence is reversed:
1. Mount a disposable tip on the pipette shaft nozzle.
2. Press the plunger button fully to the SECOND STOP.
3. Immerse the tip in liquid and allow the button to return
slowly to the resting position. Wait a moment for the liquid
column in the tip to reach equilibrium.
4. Wipe any excess liquid from the outside of the tip without
touching the orice.
5. To dispense, rest the end of the tip against the vessel wall
and press the plunger to the rst stop. Hold this position
for a few seconds, or long enough for the liquid column to
reach equilibrium again.
6. Remove the tip from the receiving vessel without blowing
out the remaining liquid.
7. Return excess sample in the tip to the original sample
container, if desired. Discard the used tip.
Troubleshooting
First Stop
Blowout
Rest Position
Step 2 Step 3 Step 5 Step 7
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