Biotage®V-10 Touch User Manual | © Biotage 2019
Prepare and Start the System
5. Press Tube and Vessel in Position to start the drain
operation and to perform the condenser start-up.
6. Please wait for the condenser to reach its target temperature
(-25 °C). This can take up to 20 minutes.
Note: If the system is used before the condenser temperature is
below -20°C, the evaporation performance will be compromised.
If the condenser is already cold, i.e. the system has recently
been operated, the condenser start-up step is skipped.
Defrost and Drain the Condenser
As the condenser accumulates ice it becomes less efficient.
If ice has built up, a defrost operation must be performed.
See instructions on page 22. After the defrost operation is
completed, the condenser will be drained.
The condenser is automatically drained when the system
is started, after each evaporation, and after each defrost
operation. It is also possible to drain the condenser manually;
see instructions on page 22.
When using V- Touch Control Centre, it is possible to
automatically defrost the condenser after a certain amount of
sample solution has been evaporated using the liquid handler.
If enabled, the automatic defrost will be performed after a job is
completed and not in the middle of a job. See "Define Biotage®
V- Touch Control Centre Settings" on page 13.
Prime the System
Warning
»
Handle chemical and liquid waste according to the Safety Data
Sheets and to local/national guidelines on laboratory safety
procedures.
If the system is equipped with a built-in sample pump, use the
prime function to 1) remove any air bubbles from the pump
and tubing by flushing them with system solvent or 2) empty
the pump and tubing of system solvent used in the previous
operation and fill them with a new system solvent.
Note: If a liquid handler is connected to the system, see "Prime
Using Biotage®V- Touch Control Centre" below.
Note: If you need to abort a prime operation, e.g. in case of
leakage, switch off (O) the system using the mains switch on the
left side of the system.
1. If necessary, clean the tubing that is connected to the
VIAL port on the pump manifold as described on page 23
before priming.
2. Insert the system solvent inlet tube into a suitable system
solvent bottle.
Note: The system solvent should be miscible with the
previous sample’s system solvent and compatible with the
next sample solution to be evaporated.
3. Insert the sample inlet tube, which is connected to the
SAMPLE ROBOT port on the pump manifold, into a clean and
empty waste container.
4. Select Maintenance from the menu bar on the touch screen.
5. Press Prime.
6. Press the Prime Volume field and enter the amount of
system solvent to be used. 45 mL is the recommended
minimum volume.
Note: The prime rate is defined in the Sample Pump view;
see "Define Sample Pump Settings" on page 5.
7. Press Prime and then Tube and Waste Container in Position
to start the prime operation.
8. When the prime operation is completed, the text “System
Ready” is displayed in the top right corner of the touch
screen. Remove and empty the waste container.
Prime Using Biotage®V- Touch Control Centre
1. If necessary, clean the tubing that is connected to the VIAL
port on the pump manifold as described on page 23
before priming.
2. In the Controller application, select Settings... from the File
menu. The Settings dialog opens.
3. Set the Needle Drain Volume to 40 mL and the Needle Wash
Volume to 5 mL.
4. Insert the system solvent inlet tube into a suitable system
solvent bottle.
Note: The system solvent should be miscible with the
previous sample’s system solvent and compatible with the
next sample solution to be evaporated.
5. Click .
6. When the prime operation is completed, the text “Completed”
is displayed in the top right corner of the software. If
necessary, remove and empty the waste container.
7. Change the Needle Drain Volume and Needle Wash Volume
parameters back to the desired settings for when cleaning
the needle. Recommended volumes are 10 and 5 mL.
