Biosystems X-CLARITY Manuel utilisateur

X-CLARITY™

Tissue Clearing System

User Manual

www.logosbio.com

LBSM-0005

V160726-01

The contents of this document may be changed in the future without advance notice.

All copyrights of this manual are owned by Logos Biosystems.

The X-CLARITY™ Tissue Clearing System is a set of laboratory electrical instruments for scientific research use only.

It is not a medical, therapeutic, or in vitro diagnostics device.

The WEEE (Waste Electrical and Electronic Equipment) symbol indicates that users of this system have the

responsibility of returning and disposing of WEEE in an environmentally friendly manner. Follow the waste

ordinances of your region for proper disposal provisions.

The CE mark indicates that this system conforms to all applicable European Community provisions for which

this marking is required. Users must be aware of and follow the conditions described in this manual for

operating the system. The protection provided by the system may be impaired if the system is used in a manner

not specified by this manual.

Protective earth (Ground)

FCC

COMPLIANCE

This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part

15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference

when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate

radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause

harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause

harmful interference in which case the user will be required to correct the interference at the user’s own expense.

Table of Contents

Table of Contents.......................................................................................................................................................................................2

Safety Information .....................................................................................................................................................................................3

Environmental Conditions for Operation .................................................................................................................................................3

1 Introduction .....................................................................................................................................................................................4

1.1 Product Overview ..................................................................................................................................................................4

1.2 Product Contents...................................................................................................................................................................4

1.3 Product Specifications...........................................................................................................................................................5

1.3.1 X-CLARITY™ETC Chamber............................................................................................................................................5

1.3.2 X-CLARITY™ETC Controller ..........................................................................................................................................5

1.3.3 X-CLARITY™Pump ........................................................................................................................................................5

1.3.4 X-CLARITY™Reservoir ..................................................................................................................................................5

2 Product Description.........................................................................................................................................................................6

2.1 X-CLARITY™ETC Chamber......................................................................................................................................................6

2.2 X-CLARITY™ETC Controller ....................................................................................................................................................7

2.3 X-CLARITY™Pump...................................................................................................................................................................8

2.4 X-CLARITY™Reservoir.............................................................................................................................................................8

3 Installation........................................................................................................................................................................................9

3.1 Multi-Cable: ETC Controller and the ETC Chamber .............................................................................................................9

3.2 Temperature Probe Connector: Temperature Probe and the ETC Controller...................................................................9

3.3 Peristaltic Pump Tube: Reservoir Outlet and the ETC Chamber Inlet.................................................................................9

3.4 Snap-Lock Connector Tube: ETC Chamber Outlet and the Reservoir Inlet ......................................................................10

3.5 Power Cords.........................................................................................................................................................................10

4 Running...........................................................................................................................................................................................11

4.1 Buffer Flow...........................................................................................................................................................................11

4.2 Sample Insertion..................................................................................................................................................................11

4.3 Tissue Clearing .....................................................................................................................................................................12

4.4 Chamber Opening................................................................................................................................................................13

4.5 Buffer Replacement.............................................................................................................................................................13

5 Maintenance and Troubleshooting...............................................................................................................................................14

5.1 ETC Chamber Cleaning ........................................................................................................................................................14

5.2 Snap-Lock Connector Cleaning ...........................................................................................................................................14

5.3 Troubleshooting...................................................................................................................................................................15

5.4 Error Codes ..........................................................................................................................................................................16

6 Ordering Information.....................................................................................................................................................................17

7 Purchaser Notification...................................................................................................................................................................18

Safety Information

Before using this system, read this manual carefully to ensure that you know how to operate it safely and correctly. Keep this

manual in an easily accessible location for future reference. Use the system only as specified.

1. Avoid electric shock while operating the instruments. Do not touch the components with wet hands.

2. Operate the instrument in the conditions described in the Environmental Conditions for Operation.

3. Wear proper personal protective equipment (PPE) when handling Electrophoretic Tissue Clearing Solution or X-CLARITY™

Mounting Solution to avoid exposure.

4. Use only components provided or authorized by Logos Biosystems.

5. Make sure that the input voltage is compatible with the X-CLARITY™ETC Controller power supply before use.

6. Use the power cord and AC adapter provided. Ensure that the power cords are firmly plugged into the power source and

the X-CLARITY™ETC Controller.

7. Turn the X-CLARITY™ETC Controller on only after connecting the power cord to it and the power source. Always turn the X-

CLARITY™ETC Controller off before disconnecting the power cord and/or moving the instrument.

8. Turn the power off before opening the X-CLARITY™ETC Chamber.

9. Carefully monitor tube connections. Replace tubes if leakage occurs.

10. Always use the pump to fill or drain the X-CLARITY™ETC Chamber. Do not pour liquids in or out directly.

11. Use the recommended volume of Electrophoretic Tissue Clearing Solution in the provided X-CLARITY™Reservoir.

12. Do not use organic solvents other than the Electrophoretic Tissue Clearing Solution described in this manual. Different

solvents may cause permanent and irreparable damage.

13. If components emit smoke, disconnect the power cord immediately from the power source2 and contact a local distributor

or Logos Biosystems.

14. Do not disassemble the components in any event. Disassembling the components invalidates the warranty. If a device

malfunctions, contact a local distributor or Logos Biosystems.

Environmental Conditions for Operation

Operating Power

100 ‒ 120 VAC, 2.2 A / 200 ‒ 240 VAC, 1.1 A

Frequency

50/60 Hz

Installation Site

Indoor use only

Operating Temperature

10 ‒ 30°C

Maximum Relative Humidity

20 ‒ 70%

Altitude

≤ 2,000 m

Pollution Degree

1Introduction

1.1 Product Overview

CLARITY is a method developed by scientists at Stanford University that produces structurally sound and transparent whole

tissues ready for multiple rounds of antibody labeling and imaging. The method has opened up a world of possibilities, from

tracing neural circuitry to exploring the relationship between structure and function with a global perspective.

With the CLARITY method, the biomolecules in intact tissues are covalently linked to a sturdy hydrogel network, preserving

molecular information and structural integrity. Lipid bilayers are broken up through electrophoresis in the presence of ionic

detergents, leaving behind a stable and transparent tissue-hydrogel hybrid that is chemically accessible for molecular

phenotyping.

Logos Biosystems licensed and independently developed the CLARITY technology to make significant improvements for tissue

clearing efficiency and reproducibility. The X-CLARITY™Tissue Clearing System is an all-in-one, easy-to-use solution for

electrophoretic tissue clearing. Its unique design accelerates the removal of lipids from tissues in a highly efficient manner.

Complicated wires are combined into a single cable for power supply and control. Improved electrodes generate a uniform

electric field that eliminates uneven voltages and currents. A timer allows precise control over the clearing time by automatically

turning off the current at the end of the allotted time. An active cooling system minimizes the probability of artifacts arising due

to poor temperature control. Buffer circulation ensures consistent buffering capacity, temperature control, and elimination of

electrophoretic byproducts. This advanced system ensures that tissue clearing occurs efficiently and rapidly.

1.2 Product Contents

The X-CLARITY™Tissue Clearing System contains the following components:

Component

Quantity

X-CLARITY™ETC Chamber

1 unit

X-CLARITY™ETC Controller

1 unit

X-CLARITY™Pump

1 unit

X-CLARITY™Reservoir

1 unit

Multi-Cable

1 unit

Power Cord

2 units

Snap-Lock Connector Tube*

1 unit

Peristaltic Pump Tube

1 unit

Container Holder for 1 Tissue Container

1 unit

Tissue Container

5 units

Electrophoretic Tissue Clearing Solution

12 x 1 L

*Assembly required

Upon receiving the product package, inspect its contents to ensure that all parts have been included and that no damage has

occurred during shipping. The warranty does not cover damage that may occur during shipping and handling. Any damage claims

must be filed with the carrier.

1.3 Product Specifications

1.3.1 X-CLARITY™ETC Chamber

Instrument Type

Electrophoretic chamber

External Dimensions (W x D x H)

176 mm x 128 mm x 154 mm

Internal Dimensions (W x D x H)

57 mm x 30 mm x 93 mm

Weight

2.8 kg

Note: Power to the X-CLARITY™ETC Chamber is supplied by the X-CLARITY™ETC Controller via the multi-cable.

1.3.2 X-CLARITY™ETC Controller

Instrument Type

Power supply and control

Dimensions (W x D x H)

180 mm x 340 mm x 200 mm

Weight

5.1 kg

Applicable Power

AC 100-240 V, 50/60 Hz

Power Consumption

210 W (including the X-CLARITY™ ETC Chamber)

1.3.3 X-CLARITY™Pump

Instrument Type

Peristaltic pump

Dimensions (W x D x H)

232 mm × 142 mm × 149 mm

Weight

2.4 kg

Speed

0.1-100.0 rpm

Speed Precision

0.1 rpm

Applicable Power

AC 100-240V, 50/60 Hz

Power Consumption

30 W

Note: Please see the separately supplied user manual for more information.

1.3.4 X-CLARITY™Reservoir

Material

Glass

Total Volume

2 L

Recommended Buffer Volume

1.2 L

2Product Description

The X-CLARITY™ Tissue Clearing System consists of the X-CLARITY™ ETC Chamber, X-CLARITY™ ETC Controller, X-CLARITY™ Pump,

and X-CLARITY™ Reservoir.

2.1 X-CLARITY™ETC Chamber

The X-CLARITY™ ETC Chamber is an electrophoretic device that applies an electric current to tissue samples placed in the

chamber. Plated electrodes produce a uniform electric field for consistent clearing. The chamber inlet and outlet allow for buffer

circulation, which eliminates the loss of buffering capacity, reduces the risk of overheating, and removes electrophoretic

byproducts. An integrated cooling system dissipates the heat generated during electrophoresis. A magnetic safety switch detects

the opening and closing of the safety cover. If the cover is open, the X-CLARITY™ ETC Controller cannot supply power to the ETC

Chamber.

2.2 X-CLARITY™ETC Controller

The X-CLARITY™ETC Controller controls the power, temperature, and safety for the X-CLARITY™ETC Chamber through one cable,

eliminating the need for dangerous and messy wiring.

To adjust values for the current, temperature, or timer, push the SET button located on the front panel of the ETC Controller.

The display will blink on in the following order when you continue to push the SET button: Current Temperature Timer.

Use the UP and DOWN buttons to adjust to the desired values. Press and hold down the UP/DOWN button to speed up

selection. Push the SET button again to apply the setting.

The ETC Controller ranges are:

Range

Increment

Voltage (V)

Not adjustable, max 60

N/A

Current (A)

0.2-1.5

0.1

Temperature (°C )

35-60

Timer (hours)

0.5-99.5, 100-999, CL

0.5, 1.0, CL

The timer increases in increments of 0.5 hours from 0-99.5 hours and then in 1 hour increments from 100-999 hours.

The “– ––“ setting is for continuous running of the system. The timer also includes a cleaning mode (CL), to use when cleaning

the ETC Chamber (see Section 5.1: ETC Chamber Cleaning). The timer goes from 999 CL –––0.5.

The X-CLARITY™Tissue Clearing System only works in constant current mode. We found that constant current mode leads to

more consistent tissue clearing. The recommended constant current value is 1.5 A. Changing the current value may affect the

time and/or efficiency of clearing and may require further optimization.

2.3 X-CLARITY™Pump

The peristaltic pump circulates the buffer to maintain consistent buffering capacity and temperature. Always use the pump to fill

or drain the ETC Chamber. Do not pour liquids in directly.

Depending on how the pump has been oriented in relation to the ETC Chamber, run the pump in a clockwise (CW) or

counterclockwise (CCW) direction to either supply or drain the chamber. The Prime (▶▶) button runs the pump at maximum

speed. At 30 rpm, the pump moves approximately 50 mL per minute.

See the separately supplied pump manual for more information.

2.4 X-CLARITY™Reservoir

It is important to circulate Electrophoretic Tissue Clearing Solution during tissue clearing to maintain buffering capacity, control

buffer temperature, and remove electrophoretic byproducts from the ETC Chamber. The temperature probe attached to the

reservoir cap is connected to ETC Controller and monitors the temperature of the clearing solution.

3Installation

Place the components of the X-CLARITY™Tissue Clearing System on a clean and flat surface devoid of vibration. Leave sufficient

space around the ETC Chamber for the cooling fan to work properly.

3.1 Multi-Cable: ETC Controller and the ETC Chamber

Connect the ends of the multi-cable to the ports on the rear panels of the ETC Controller and Chamber. Check the homing

grooves and the orientation of the pins on the ends of the multi-cable for proper orientation. Fasten the nuts tightly to ensure a

tight connection.

3.2 Temperature Probe Connector: Temperature Probe and the ETC

Controller

Screw the cap onto the reservoir. Make sure the temperature probe and reservoir outlet tube are both inside the reservoir.

Plug the temperature probe connector into the temperature probe port on the rear panel of the ETC Controller. Check the

homing groove on the end of the connector and the thickness of the pins in the port for proper orientation.

3.3 Peristaltic Pump Tube: Reservoir Outlet and the ETC Chamber Inlet

Attach one end of the peristaltic pump tube to the ETC Chamber inlet located on the front panel of the ETC Chamber. Attach the

other end to the reservoir outlet (the reservoir outlet is connected to tubing that extends into the reservoir). Make sure there is

a tight seal at each connection to prevent buffer leakage.

Pull the pump head lever to the left to open the compression block. Lift the clamps on both sides of the pump head. Load the

middle of the peristaltic pump tube into the pump head. Make sure the tube is between the rollers and the compression block.

Release the clamps. Pull the pump head lever to the right to close the compression block.

The provided peristaltic pump tube has a tubing life of 3,000 hours. Only use tubes provided or approved by Logos Biosystems.

Table des matières

Autres manuels biosystems Équipement de laboratoire

biosystems

biosystems A15 Manuel utilisateur

Manuels Équipement de laboratoire populaires d'autres marques

Qiagen

Qiagen DML 3000 Manuel utilisateur

Queensgate

Queensgate NANOSCAN OP400 Manuel de programmation

Conviron

Conviron GEN1000-GE Manuel utilisateur

Parr Instrument

Parr Instrument 4560 Mode d’emploi

IKA

IKA IC control Manuel utilisateur

Integra

Integra DOSE IT Manuel utilisateur

Agilent Technologies

Agilent Technologies 5800 ICP-OES Manuel utilisateur

Endress+Hauser

Endress+Hauser Cleanfit CPA875 Manuel utilisateur

NI PXI-5422 Manuel

Collomix

Collomix Aqix Manuel utilisateur

SPEX SamplePrep

SPEX SamplePrep 6875 Freezer/Mill Series Manuel utilisateur

Ocean Insight

Ocean Insight FLAME-NIR+ Manuel utilisateur

Parker

Parker ALIGN-MG-NA Manuel utilisateur

BD 644787 Manuel utilisateur

DENTAURUM

DENTAURUM Compact Megaplus Manuel utilisateur

Biuged Laboratory Instruments

Biuged Laboratory Instruments BGD 626 Manuel utilisateur

VWR

VWR SAS Super IAQ Manuel utilisateur

illumina

illumina MiSeqDx Manuel utilisateur

biosystems X-CLARITY Manuel utilisateur

Manuels Équipement de laboratoire populaires d'autres marques