Biometra TGRADIENT 48 Manuel utilisateur
TGRADIENT Manual ___________________________________________________________ 1
Manual
Software Version 4.20
May 2003
Order-No.
050-800 TGradient 48
050-801 TGradient 96
Biometra
biomedizinische Analytik GmbH
Rudolf-Wissell-Str. 30, D-37079 Goettingen
Tel.: 0551/50 686-0; Fax: 0551/50 686-66
email: [email protected]
http://www.biometra.com
TGRADIENT Manual ___________________________________________________________ 2
1 Introduction
The TGRADIENT is the new high end thermocycler in the Biometra product line. It was our goal
to develop an instrument that provides fast optimization of reaction parameters as well as a high
performance for daily routine analysis. Both could be realized by a completely new block
architecture and the latest Peltier technology.
To increase the specificity of a reaction, the annealing temperature is one of the crucial
parameters. With the new TGRADIENT different annealing temperatures can be tested within a
single run. A gradient with a maximum range of 40°C can be set at any program step. We have
taken strong efforts to create a gradient as linear as possible. At the same time it was very
important to ensure homogenous temperature distribution within each row. The temperature data
for each row of the block can be retrieved through the help menu.
The new TGRADIENT is an outstanding instrument. In addition to the advanced temperature
gradient it provides high speed and reliable accuracy for routine applications. The silver block
features very high heat conductivity and ensures fast ramping speeds and a fast temperature
equilibration.
The new TGRADIENT will speed up your laboratory work because it saves time not only in
optimizing new reactions but also in your routine work.
TGRADIENT Manual ___________________________________________________________ 3
2 Before you start
2.1 Safety warnings
Before connecting the unit to the power source please ensure that the
voltage selector at the back side of the instrument is set to the required
voltage.
Danger of electric shock! Unplug the power cable before you open the
TGRADIENT Thermocycler.
It is not necessary to utilize oil into the opening of the block in order to
improve the heat transfer between the block and the sample tubes. If you
still decide to use oil, do not use silicon oil. Mineral oil may be used.
2.2 Warning symbols
The thermoblock and the heated lid will reach high temperatures
during operation. Both thermoblock and heated lid can burn you.
Rapid heating of the thermoblock can cause liquids to boil
explosively. Always wear safety goggles during operation. Close the
lid before starting a program.
Ensure that both the rear and bottom ventilation slits of the rear and
bottom of the instrument are unobstructed. Insufficient ventilation
can cause overheating of the instrument.
TGRADIENT Manual ___________________________________________________________ 4
3 First steps with the TGRADIENT
3.1 The TGRADIENT Thermocycler front View
Wheel for the height
adjustment
of the heated lid.
Keypad
Graphical display
Power switch
Knob for release
of the lid
TGRADIENT Manual ___________________________________________________________ 5
3.2 The TGRADIENT rear view
Parallel
printer port
RS-232
port
Power cord
plug
Fuse holder
for 2 fuses,
for specifications of fuses
see type label on the
instrument´s backside
Type label including:
serial number
specifications of fuses
TGRADIENT Manual ___________________________________________________________ 6
3.3 The TGradient control panel
798
123
456
.0-
ABCD
Pow er
Cursor keys:
For free navigation in the
programming spreadsheet.
Use to scroll through lists.
Function keys:
the corresponding functions are
specified in the screen.
Power switch:
backlit = power on
Easy to clean
keyboard
Graphical display
TGRADIENT Manual ___________________________________________________________ 7
3.4 Initial self test
After switching on the TGRADIENT the serial number of the instrument and the software version
is displayed.
TGradient
Serial No 1234567
Vers. 4.00 op
The TGRADIENT checks all programs in all directories (so called RAM check).
3.5 The TGradient display
The TGRADIENT display provides information about the instrument and about the status of the
active program.
prog
step
temp[°C] 25.2°C
time
lid 25.4°C
A ? B start/stop C programs D +
The individual function of the special keys A , B , C and D are specified in the display and
depend on the context.
3.6 Handling of the adjustable heated Lid
To achieve optimum pressure on the tubes the TGRADIENT is equipped with an height adjustable
heated lid.
Close the lid:
After the samples have been placed in the block close the lid. Turn the wheel clockwise until you
hear a clicking noise. In this mode the pressure will not increase further, even when you keep on
turning the wheel.
Note: The pressure of the lid has been optimize for a fully loaded block. If only very few tubes
are loaded to the block you should place dummy tubes in the four corner positions to avoid
damage of tubes by excessive pressure.
Open the heated lid:
First: Release pressure by turning the wheel counter clockwise. As soon as there is no more
resistance the pressure has been released. Then: now you can open the lid with the knob.
Important: The lid should not be opened under pressure because this leads to damage of the
locking mechanism.
TGRADIENT Manual ___________________________________________________________ 8
3.7 Releasing wheel in case of blocked lid
Note: When the lid is in the extreme up or down position, it may happen that the wheel is
uncoupled. In this situation the clutch mechanism is active in both directions (clicking noise in
either direction). To unlock wheel, press metal pin with a ballpen and turn wheel carefully. This
pin overcomes the automatic clutch mechanism. Thus, care must be taken not to apply excessive
pressure.
Lid arrested in upper
position:
carefully turn wheel with
pressed pin clockwise, until
you feel normal resistance (no
more clicking noise, clutch is
released). Release pin and
turn lid down, until the clutch
mechanism is activated
(clicking noise, optimum
pressure applied).
Lid arrested in down
position:
carefully turn wheel with
pressed pin counter
clockwise, until you feel
normal resistance (no more
clicking noise, clutch is
released). Release pin and
turn wheel counter clockwise
until pressure is completely
released. Open lid.
IMPORTANT: WHEN THE CLUTCH MECHANISM IS ACTIVE (OPTIMUM
PRESSURE IS APPLIED), NEVER USE PIN TO FURTHER INCREASE PRESSURE.
THIS WILL LEAD TO DAMAGE OF TUBES AND INSTRUMENT!
TGRADIENT Manual ___________________________________________________________ 9
4 Creating a Program
4.1 Select a directory
In the TGRADIENT programs can be stored both in the main directory and in individual
subdirectories. For easy identification of the selected storage place, the subdirectories can be
named (see section 4.3).
Start with the main screen.
Prog
step
temp[°C] 25.2°C
time
lid 25.4°C
A ? B start/stop C programs D +
Press [C programs] to enter the edit mode.
½-3. subdirect.:
½-4. subdirect.:
Main direct.--- ½-5. Subdirect.:
½-6. subdirect.:
½-7. subdirect.:
A ? B C quit D enter
You are now in the main directory. The structure of the subdirectories is displayed.
4.2 Select a program store
Programs can be stored in the main directory or in subdirectories.
To create a program in the main directory press [D enter].
To access a subdirectory move right with the cursor key ðand select a subdirectory with the
cursor keys ñò. The selected directory will be highlighted.
½-3. subdirect.:
½-4. subdirect.:
Main direct.--- ½-5. Subdirect.:
½-6. subdirect.:
½-7. subdirect.:
A ? B C quit D enter
TGRADIENT Manual __________________________________________________________ 10
4.3 Enter name for subdirectory
The process for naming a subdirectory and a program is the same. For information about naming
programs see section 4.4.
Press [D enter] to enter the subdirectory
directory: 5
program no. Name:
edit
A list B C quit D enter
Press [A list] to view a list of all existing files and empty memory in this directory.
Note: If neither program name nor the word “empty” is displayed, this place may be occupied by
a program without name. For the programming of new protocols choose an empty memory.
Existing programs that are not needed any more can be deleted to provide new memory.
0 empty
1 empty
2 empty
3 empty
4 empty
A list B forward C quit D enter
Scroll through the list with cursor keys ñò and confirm memory with [D enter].
4.4 Enter a program name
Each program is specified by a program number and a subdirectory number. To make retrieval of
a program easier, you can enter a name for each program existing of letters, numbers and
symbols.
Directory: 3
program no. 0 Name:
Ù
ABCDEFGHIJKLMNOPQRST
UVWXYZ-()#°C/,áñ&+.%!
A ABC B files C name OK D enter
To enter a name for your program press [A ABC].
Directory: 3
program no. 0 Name:
Ù
ABCDEFGHIJKLMNOPQRST
UVWXYZ-()#°C/,áñ&+.%!
A name B blank C quit D enter
Choose a letter from the alphabet with cursor keys ïðñò
Ce manuel convient aux modèles suivants
3
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