Contents | v
Synergy HT Operator’s Manual
Fluorescence Tests........................................................... 76
Required Materials....................................................... 77
Test Solutions............................................................. 78
Procedure .................................................................. 79
Results Analysis .......................................................... 79
Troubleshooting .......................................................... 80
Pipette Map ................................................................ 81
Gen5 Protocol Reading Parameters ................................ 82
Fluorescence Tests Using Methylumbelliferone................. 83
Dispense Module Tests ..................................................... 86
Required Materials....................................................... 87
Alternate Test Solutions ............................................... 88
Test Setup ................................................................. 89
Test Procedure............................................................ 89
Results Analysis .......................................................... 91
Creating the Test Protocols........................................... 92
Chapter 5: Preventive Maintenance....................................93
Recommended Maintenance Schedule................................. 94
Overview ................................................................... 94
Daily Cleaning for the Dispense Module .......................... 94
Recommended Maintenance Schedule ............................ 95
Warnings & Precautions.................................................... 96
Cleaning Exposed Surfaces................................................ 97
Inspect/Clean Excitation and Emission Filters....................... 98
Flush/Purge the Fluid Path ................................................ 99
Running a Dispense Protocol (Optional) .............................100
Empty/Clean the Tip Priming Trough .................................101
Clean the Priming Plate....................................................101
Clean the Internal Components.........................................102
Required Materials......................................................103
Removing the Reader’s Shroud.....................................104
Removing the Internal Tubes and Injector Heads............106
Cleaning the Internal Tubes and Injector Heads..............109
Cleaning the Optical Probes .........................................110
Cleaning the Reader’s Internal Surface..........................118
Reassembling the Components.....................................119
Performance Check.....................................................120
Appendix A: Decontamination ..........................................123
Purpose.........................................................................124
Required Materials ..........................................................125
Procedure for Models without Injectors ..............................126
Routine Procedure for Models with Injectors .......................127
