BI 399 Manuel utilisateur
Air-Jacketed Large Capacity
Flo-Thru CO2Incubators
Model No.
399
399-1
399GWH
057-641-00 • 6/8/05
Safety Information ........................................................................................................................................................3
Alert Signals ..........................................................................................................................................................3
Specifications ..............................................................................................................................................................4
Electrical Requirements ........................................................................................................................................4
Temperature Range ..............................................................................................................................................4
Temperature Control..............................................................................................................................................4
Uniformity in Chamber ..........................................................................................................................................4
Gas Flow Rates ....................................................................................................................................................4
Volume ..................................................................................................................................................................4
Humidity ................................................................................................................................................................4
Dimensions ............................................................................................................................................................4
Shelves ..................................................................................................................................................................4
Net Weight ............................................................................................................................................................4
Options ..................................................................................................................................................................4
Unit’s Environmental Operating Conditions ..........................................................................................................5
Unpacking and Installation ..........................................................................................................................................6
Shipping Carton ....................................................................................................................................................6
Location ................................................................................................................................................................6
Electrical Power ....................................................................................................................................................6
Shelf Positioning ..................................................................................................................................................6
Adjustable Screw Guards for Protecting Back of Unit ..........................................................................................7
Hooking Up Water ................................................................................................................................................7
Connecting Condensate Drain to the Incubator ....................................................................................................7
Air/CO2 Hook-Ups ................................................................................................................................................8
CO2 Connection: Standard and Siphon Type CO2 Gas Cylinders ......................................................................9
Operation....................................................................................................................................................................10
Auto Tune ............................................................................................................................................................12
For Best Results ..................................................................................................................................................12
Auto-Tuning Procedure........................................................................................................................................12
Temperature Calibration ......................................................................................................................................13
Setting the Hi-Limit Thermostat ..........................................................................................................................14
Loading the Incubator..........................................................................................................................................14
Air/Gas Ratio ......................................................................................................................................................15
Humidity Adjustment............................................................................................................................................16
CO2 Sampling/Measurement ..............................................................................................................................16
Air and CO2 (or Nitrogen) ..................................................................................................................................16
Humidification ......................................................................................................................................................17
Humidifying without the Optional Humidifier ................................................................................................17
Humidifying with the Optional Humidifier......................................................................................................17
About the Kind of Water to Use ....................................................................................................................18
Demineralizer Cartridge................................................................................................................................19
Operation of Humidification System ............................................................................................................20
Periodic Cleaning and Inspecting of Humidifier............................................................................................20
Humidity Controller ......................................................................................................................................22
Auto Tune ....................................................................................................................................................23
Offset Humidity Calibration ..........................................................................................................................23
Operating Suggestions ................................................................................................................................24
Hints on Using Fyrite ..........................................................................................................................................25
Taking a Fyrite Reading...................................................................................................................................... 26
Kwik Inject Automatic CO2 Recovery System ....................................................................................................29
Built-In Compressed Air Supply ..........................................................................................................................29
Maintenance ..............................................................................................................................................................30
Replacing Temperature and/or Humidity Controller ............................................................................................30
CO2 and Air Bubbler............................................................................................................................................30
Adjusting Automatic CO2 Recovery System ......................................................................................................31
Replacement Parts ....................................................................................................................................................33
Ordering Procedures ..................................................................................................................................................34
Decontamination Statement ......................................................................................................................................35
Two Year Limited Warranty ........................................................................................................................................36
Table of Contents
The Barnstead|Lab-Line Large-Capacity, 34 cu. ft. (963
liter), Flo-Thru CO2 Incubator has been specially
designed for laboratories that handle a high volume
of work. The unit has been factory tested and needs only
to be placed in a suitable location and connected to the
required electrical supply and air and CO2 (or nitrogen)
sources at a pressure of 15 psi. A blower fan assists in
maintaining uniformity of the CO2 concentration in the
chamber, as well as, minimizing any temperature gradi-
ents.
All controls are contained in the control panel. The micro-
processor- based controller maintains temperature from
slightly above ambient to 60ºC. The hydraulic hi-limit
thermostat controls temperature a few degrees above the
user established set point in the event the main controller
fails. This provides an added measure of protection to
the chamber.
The KWIK-INJECT™ system provides quicker recovery
and a more stable chamber environment. CO2 is inject-
ed into the chamber to compensate for losses incurred
during door openings. A timer on the control governs
injection time from 3 to 60 seconds.
6 perforated stainless steel shelves provide a total area of
36 sq. ft. (3 sq. m) with a load capacity of 100 lbs. (45 kg)
per shelf when the load is evenly distributed, 65 lbs. (29
kg) per shelf when the load is concentrated.
Humidification may be obtained simply by placing a pan
of deionized or distilled water on one of the shelves. A
humidifier and controller are available as factory-installed
options and require a demineralized water source. The
humidifier is controlled from the front panel and allows
regulation of the relative humidity from approximately
20% above ambient to 98% RH. A panel meter gives a
direct reading of relative humidity.
The exterior of the incubator is made from cold rolled
steel with a durable powder coated finish. The interior
and door are made of stainless steel. Inside the door and
between the double walls is 3-inch thick glass wool insu-
lation that helps hold heat loss to a minimum and pro-
motes temperature uniformity in the chamber.
Model 399GWH has an optional humidifier with controller.
Safety Information
Warning
Warnings alert you to a possibility of
personal injury.
Caution
Cautions alert you to a possibility of
damage to the equipment.
Note
Notes alert you to pertinent facts and
conditions.
Hot Surface
Hot surfaces alert you to a possibility of
personal injury if you come in contact
with a surface during use or for a period
of time after use.
Alert Signals
3
4
Electrical Requirements
399, 399GWH: 120 Volts, 50/60 Hz, 1400 Watts, 11.7 Amps
399-1: 230/240 Volts, 50/60 Hz, 1400 Watts, 5.8 Amps
Temperature Range
Ambient +5°C to 60°C
Temperature Control
±0.5°C
Uniformity in Chamber
±0.5°C
Gas Flow Rates
Air: 1.0 to 14.0 liters/minute
CO2: 0.1 to 1.2 liters/minute
Volume
34 cu. ft. (963 liters)
Humidity
Range: ambient to 98% RH
Accuracy: ±3% RH
Dimensions
Exterior: 42”W x 35”D x 91”H (107cm x 89cm x 231cm)
Chamber: 36”W x 25”D x 65”H (91cm x 64cm x 165cm)
Specifications
Shelves
6 perforated, stainless steel
Net Weight
980 lbs. (445 kg)
Options
H: Humidity Control
L: Left Hand Door
P: Built-in compressed air supply
GW: Glass Window 12” x 48” (30 x 122 cm)
Unit’s Environmental Operating Conditions
Pollution Degree: 2
Installation Category: II
Altitude: 2000 meters MSL (Mean Sea Level)
Humidity: 80% maximum, non-condensing
Electrical Supply: 120VAC or 240VAC
Voltage Tolerance: ±10% of normal rated line
Temperature: 15°C to 40°C
Product Usage: This product is intended for use indoors only
5
SPECIFICATIONS
6
Shipping Carton
The shipping carton should be inspected upon delivery.
When received, carefully examine for any shipping dam-
age before unpacking. If damage is discovered, the deliv-
ering carrier should both specify and sign for the damage
on your copy of the delivery receipt.
Open the carton carefully making certain that all parts are
accounted for before packaging materials are discarded.
After unpacking, if damage is found promptly report it to
the carrier and request a damage inspection promptly.
IMPORTANT: Failure to request an inspection of damage
within a few days after receipt of shipment absolves the
carrier from any liability for damage. You must call for a
damage inspection promptly.
Location
Select a location for the incubator that is free of drafts
and extraneous vibrations. The unit should be level.
Electrical Power
Turn all controls to the off position. Connect the power
cord of the incubator to an outlet providing the power
characteristics specified on the unit's nameplate or in this
manual.
Shelf Positioning
The 6 perforated stainless steel shelves are adjustable on
1/2" (12 mm) centers. Place the shelf support clips
where desired and then set shelves in place. Shelves
and support clips can be ordered to provide additional
shelf space.
Unpacking and Installation
Warning
Do not use in the presence of flam-
mable or combustible materials or
explosive gases. Do not use in the
presence of pressurized or sealed
containers. Fire or explosion may
result, causing death or severe
injury.
Warning
Do not heat any substance above a
temperature that will cause it to emit
toxic fumes. Death or severe injury
may result.
Warning
Use only an inert gas such as car-
bon dioxide in the incubator. Do not
under any circumstance inject oxy-
gen or other explosive gas or mix-
ture into unit. Failure to observe
these precautions can result in
explosion and/or fire and serious
injury or death to personnel and
property damage.
7
Adjustable Screw Guards for
Protecting Back of Unit
Exercise care in placing the back of the unit against a wall
to avoid damage to CO2 tubing and related attachments.
To protect against this, 2 adjustable screw guards on the
back are used to establish a buffer zone for the CO2 tubing.
If unit is shipped upright, guards are in place; if shipped flat,
guards are furnished loose and must be inserted.
Hooking Up Water
Connect supply water line to the 1/4” NPT fitting located on
the inlet side of the D. I. cartridge. The recommended
water pressure setting on the regulator gauge should be
adjusted to read between 10 and 15 psi.
Connecting Condensate Drain to
the Incubator
1/2” drain lines are located in back of the incubator.
UNPACKING AND INSTALLATION
8
Turn all flow meters on the incubator fully clockwise (closed).
Connect air and CO2 supply lines to their respective fittings on
the back of the unit. Use 1/4" (6 mm) ID tubing, flexible metal
hose, etc., (meeting local codes), with filters to ensure that the
air and CO2 are free of contaminants, for gas lines.
Set the supply regulators at 15 psi. Check all of the connec-
tions for leaks.
UNPACKING AND INSTALLATION
Air/CO2 Hook-Ups
Turn the power switch to OFF and insert the plug into the outlet.
To Air Fitting
To CO2 Fitting
100% Air 100% CO2
1/4" Hose Barb
Connections
Set pressure at 15 psi.
Pressure regulators and gauges must be
installed on each tank. Check local gas suppli-
er for proper regulators.
If air pump is used instead of tank, air must be
filtered to remove water, oil and dirt.
Warning
High concentrations of carbon diox-
ide produce metabolic abnormalities,
disturbances of the central nervous
system and cardiac instability.
Unconsciousness may occur at con-
centrations above 10%.
Note
It is our recommendation that this
unit run 24 hours empty before intro-
ducing media to be incubated. This
will facilitate ease of servicing if
required as well as nullify the need
to decontaminate the chamber in the
event a problem occurs.
This unit cannot be accepted for
servicing or credit by Barnstead
International unless accompanied by
a completed Certification of
Decontamination form, included at
the end of this manual.
Standard Type (Correct) Siphon Type (Incorrect)
1
2
3
12
3
4
5
6
7
8
1. Compressed Gas Association #320
connector.
2. Gas head space.
3. Liquid CO2 filled to 68% equal weight
of water that cylinder would hold at
60ºF.
1. Warning tag indicating that cylinder is siphoning type.
2* Aluminum ring.
3* Gold band.
4* Stamp or marking on cylinder: "Siphon" or "Eductor
Tube".
5* Valve should be of special type for service.
6* Withdrawal tube draws up the liquid CO2.
7* Gas head-space.
8* Liquid CO2 filled to 68% equal weight of water that
cylinder will hold at 60ºF.
*Note: Some gas suppliers will have no markings to indicate
an “Educator Tube” or “Siphon” type cylinder. Be sure to order
DRY, LAB-GRADE CO2.
Be sure to obtain a dual-stage regulator from the gas supplier
for the CO2 tank that is to be installed according to local
codes. Connect 1/4" ID (6.35 mm) flexible tubing to the regu-
lator. Connect the other end of the tubing to the unit's CO2
inlet (located on the back top panel). Insert tubing over hose
barb and fasten with an appropriate clamp to assure a proper
connection. Adjust the regulator for the pressure recommend-
ed on the previous pages when CO2 is to be injected into
chamber. For optimum results, do not exceed or reduce this
pressure.
CO2 Connection: Standard and Siphon Type CO2 Gas Cylinders
9
UNPACKING AND INSTALLATION
10
Operation
Amicroprocessor-based automatic tuning controller with a
platinum RTD temperature sensor provides a display of
either chamber or set point temperatures. The micro-
processor used in the unit automatically tunes PID
parameters to fit the characteristics of individual thermal
systems.
Auto-tune is a user-initiated function. It provides a tune-at-
temperature function that means the controller runs the
auto-tune program when the incubator is at set point.
Program parameters are retained in the nonvolatile mem-
ory in the event that there is power failure or interruption.
1 2 3 4 5 6 7 8 9
1. Power Switch
2. Hi-Limit Thermostat
3. Hi-Limit Thermostat Status Lamp
4. Temperature Controller
5. Kwik-Inject Control Dial
6. Kwik-Inject Status Lamp
7. Sample Access Port
8. Air Flow Meter
9. CO2 Flow Meter
Ce manuel convient aux modèles suivants
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