Bertin Technologies PRECELLYS 24 Manuel utilisateur

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NEW GENERATION Tissue homogenizer

SAMPLE PREPARATION & CELLLYSIS

USER MANUAL / MANUEL UTILISATEUR

PRECELLYS®24 & PRECELLYS®24-DUAL

Precellys®24 &Precellys®24-Dual

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USER MANUAL

Manufactured by:

BERTIN TECHNOLOGIES

Parc d’activités du Pas du Lac

10 bis, avenue Ampère - BP 284

78053 Saint Quentin en Yvelines Cedex

France

Tel: + 33 (0)1 39 30 60 00

Mail: precellys@bertin.fr

User manual code: 03720-800-DU004-I

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TABLE OF CONTENTS

1. INTRODUCTION

1.1 Safety Information

1.2 Warranty

1.3 Precellys®24/ Precellys®24-Dual’s Reference

1.4 Manufacturer Information

1.5 Technical Support

2. DESCRIPTION OF THE PRECELLYS®24/ PRECELLYS®24-DUAL

2.1 Product Overview

2.2 Tube Motion

2.3 Lysing kits

2.4 Programming

2.5 Presentation of the Keypad

2.6 Technical Features

3. TRANSPORT / STORAGE

3.1 Transport

3.2 Storage

4. INSTALLATION

4.1 Unpacking

4.2 Installation and Connecting the Power Supply

5. INSTRUCTIONS FOR USE

5.1 Preparing Samples

5.2 Starting a Run

6. ADJUSTING PARAMETERS

6.1 Accessing the Parameter Adjustment Menu

6.2 Parameters Adjustment Menu

6.3 Operating Ranges for Parameter Adjustments

7. ALARMS

7.1 Alarm Messages before Starting a Run

7.2 Run Interruption by Alarm Messages

7.3 What to Do When an Alarm Message Occurs

8. MAINTENANCE

8.1 Cleaning and Decontamination

8.2 Troubleshooting Guide

8.3 Replacing Spare Parts

8.4 Service Menu

9. REPARATION / ELIMINATION

9.1 Reparation

9.2 Elimination

Precellys®24 &Precellys®24-Dual

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USER MANUAL

This user manual includes the

required information regarding

unpacking, operation and

maintenance of the Precellys®24/

Precellys®24-Dual lyser /

homogeniser.

The product’s technical

speciﬁcations and the

following information are

subject to change without

prior notice.

1 Introduction

1.1 Safety Information

This user manual must be read carefully before operating the Precellys®24/

Precellys®24-Dual lyser/homogeniser.

If there is any doubt or concern about the safety of the equipment,

please contact your local distributor.

Bertin Technologies is not responsible for any damage or injury that may oc-

cur as a result of operating the instrument in a different way to that stated in

this document.

Symbol signiﬁcation

Danger

Necessity to respect the safety instructions

Biological Danger

The no respect of the symbol can cause risks of personal and

environmental injury.

This symbol is delivered separately and must be pasted in front

of the unit for an utilisation with infectious samples.

Separate collection of electrical and electronic equipments to

the directive 2002/96/EC.

Necessity to respect the measures in effect.

1.1.1 Use of the lysing kits

Depending on the parameters of the Precellys®24/ Precellys®24-Dual (speed,

number of cycles, cycle duration, waiting time between two cycles), an impor-

tant overheating of the lysing tubes may lead to their sudden degradation.

To prevent any risk to the user when running thePrecellys®24/ Precellys®24-

Dual it is scrupulously recommended to use the lysing kits produced by Bertin

Technologies and respect the use limits deﬁned for each lysing kit. Those

limits specify maximum level for parameters (speed, number of cycles, cycle

duration, waiting time between two cycles, etc). It will ensure good biological

samples preparation.

The use limits of every Lysing kits are available on the website www.precellys.

com or in each Bertin Technologies Kits.

1.1.2 Risk of Electric Shock

Although this equipment is fully insulated and grounded, it is important for all

users to be aware of the potential hazard of using liquids close to a power

supply. If any liquids are spilled, disconnect immediately the instrument from

the main power supply (remove the power cord from the AC power input on

the rear panel) and clean the equipment and the surrounding area.

Do not reconnect the equipment until it has been fully inspected.

INTRODUCTION

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Ne pas ouvrir le capot lors

du fonctionnement.

Do not open the cover while

running.

WARNING :

INTRODUCTION

1.1.3 Incorrect Operation

Operating this equipment in other ways than those detailed in this user manual

may impair the protection of the unit.

• Do not turn the unit upside down: the Precellys®24/Precellys®24-Dual must

always rest on its 4 feet for fear of damaging internal components or break-

ing plastic casing.

• Do not operate the unit when the casing is removed; potentially lethal vol-

tage exists within the instrument.

• Do not operate the unit with the safety ground disconnected.

• Do not install unauthorised cards, spare components or accessories as this

may impair the safety of the unit and the warranty will be void.

• Do not overﬁll the tubes as this may lead to liquid contamination and impair

the safety of the unit.

• Check that the voltage indicated on the rear panel of the unit matches to

the local power supply.

• Check that the power cord is properly plugged-in. Colour codes are as

follows:

1.1.4 Fuses

The equipment contains one replaceable external fuse located on the rear pa-

nel. If the fuse is to be replaced, please use the following fuse 5x20 – T 10 A

– H 250 V. It can easily be replaced by the user with a screwdriver (see §8.2).

1.1.5 Biological Risks

Wear gloves when handling samples and follow strictly all of the safety

instructions related to bio-hazardous agents to prevent any risk of

contamination. The waste produced by the normal operation of the

instrument must be disposed of in biological waste containers and handled by

specialised companies.

1.1.5 Biological Risks

Wear gloves when handling samples and follow strictly all of the safety instruc-

tions related to bio-hazardous agents to prevent any risk of contamination. The

waste produced by the normal operation of the instrument must be disposed of

in biological waste containers and handled by specialised companies.

1.1.6 Warning Symbols

The symbols on the right can be found in various places on the Precellys®24/

Precellys®24-Dual.

Please follow instructions associated with these symbols.

The equipment can only be repaired by either the authorised distributor or

the manufacturer.

Lire attentivement la notice avant

utilisation.

Read carefully user manual before

operating.

WARNING :

USA

Code

Black

White

Green

International

Code

Brown

Blue

Yellow & Green

Phase Live

Neutral

Earth/Ground

Precellys®24 &Precellys®24-Dual

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USER MANUAL

1.1.7 Noise Level

The equipment’s noise level is below 70 dBa when in operating mode at a

speed of 6500 rpm.

1.1.8 Electromagnetic Interference

WARNING: This is a Class A apparatus. The equipment may cause

radio-electric interference in a residential environment. In this case,

it is recommended that the user takes appropriate measures.

1.1.9 Intensive Use

If this device is used in an intensive manner (high speed, long cycle duration,

fully loaded with 24 tubes, high number of cycles), the temperature of the

system will increase. The increased temperature level may cause the thermal

security protection to function. In this case, the electrical power input of the

motor will be cut automatically to avoid the overheating of this device.

1.2 Warranty

Bertin Technologies certiﬁes that this product is free of defects at the time

of shipment.

This warranty is limited to a period of one (1) year and it does not apply to the

following parts: fuses, toric joint, containment seal and indented plate.

This warranty is not applicable in the following circumstances:

• The equipment has not been installed, operated or maintained according to

the instructions described in this user manual.

• The equipment has been repaired or modiﬁed by unauthorised personnel.

• The equipment serial number has been damaged or removed.

1.3 Precellys®24’s and Precellys®24-Dual’s References

Code: •Precellys®24: PRECELLYS 03119.200.RD000

• Precellys®24-Dual: PRECELLYS 03119.200.RD010

1.4 Manufacturer Information

BERTIN TECHNOLOGIES

Parc d’activités du Pas du Lac

10 bis, avenue Ampère - BP 284

78053 Saint Quentin en Yvelines Cedex - France

Tel: +33 (0)1 39 30 60 70

Fax: +33 (0)1 39 30 61 85

1.5 Technical Support

First, read this user manual very carefully. If you cannot solve the problem

after having used this manual, please contact the nearest distributor’s ofﬁce

or Bertin Technologies’ Customer Support Department located in France (see

the address above).

INTRODUCTION / DESCRIPTION

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2. Description of the Precellys®24/Precellys®24-Dual

2.1 Product Overview

The Precellys®24/ Precellys®24-Dual lyser / homogeniser has been designed

to lyse and to homogenise biological samples contained in grinding tubes, at

variable speeds. It can simultaneously agitate tubes with biological sample

volumes up to 7 mL at very high speed.

Precellys®24/Precellys®24-Dual’s main advantages include:

• Easy tube loading: an innovative system automatically blocks the grinding

tubes during operation.

• Easy decontamination: zones which have to be decontaminated are easily

accessible.

• Flexible and easy cycle programming (time, speed…).

• No alteration of the biological samples and no cross-contamination.

• Efﬁcient and homogeneous lysis in all tubes.

The Precellys®24/ Precellys®24-Dual lyser / homogeniser is shown hereaf-

ter:

1 - cover

2 - locking latch

3 - keypad

4 - tubes holder

5 - indented plate:

Precellys®24 : 24x2mL

Precellys®24-Dual : 12x2mL

6x7mL

6 - containment seal

7 - air opening

8 - fan

9 - On/Off button

10 - main power supply plug

11 - fuse

12 - 120V/240V switch

13 - maintenance plug

DESCRIPTION

Precellys®24 &Precellys®24-Dual

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USER MANUAL

WARNING:

2.2 Tube Motion

Thanks to the equipment’s design and symmetry, every tube of the same

size strictly follows the same motion to ensure the same level of lysis and

homogenisation for each sample.

The centre of gravity of the tubes follows a tri-dimensional path on a sphere’s

surface. While the mixture contained in grinding tubes moves in all directions,

it moves primarily in the vertical axis to allow for efﬁcient homogenisation.

The movement generated by the Precellys®24/ Precellys®24-Dual equipment

is called a “precession” movement (i.e. grinding tubes are not rotated).

Each 2 mL tube has a stroke of 8 mm projected on the main vertical axis.

Each 7 mL tube has a stroke of 8.5 mm projected on the main vertical axis.

The tubes must be compatible with the holder and must withstand 600 G of

linear acceleration during 5 minutes without suffering any deformation (see

preconisation of use § 1.1.1 and recommended references § 2.3).

Precession movement doing biological sample homogenisation also causes

an increase of temperature of the samples and the equipment.

2.3 Lysing kits Precellys®24/Precellys®24-Dual

The Precellys lysing kits range includes tubes ready-to-use containing beads

adapted to every sample type. References of the lysing kits are available on

the website www.precellys.com.

DESCRIPTION

2.4 Programming

The Precellys®24 lyser / homogeniser has been designed to operate at a

maximum speed of 6,800 rpm. The Precellys®24-Dual lyser/homogeniser

has been limited at a maximum speed of 6,500 rpm. A run consists of one or

several cycles, between which the cover must not be opened.

Speed as well as other settings can be adjusted both in one of the ﬁve pro-

grams available on the keypad:

Parameter

Speed

Number of cycles

Cycle duration

Waiting time between 2 cycles

Operating range

From 5,000 to 6,800 (6,500) rpm in increments of 100 rpm

From 1 to 3

From 5 to 90 seconds in increments of 1 second

From 5 to 120 seconds in increments of 1 second

The increase of temperature is

higher for 7 mL tubes than for

2 mL tubes.

VISIT:

www.precellys.com

to know the evolutions of the

consumables dedicated to

Precellys sample preparation

range.

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DESCRIPTION

The user can adjust four different homogenisation settings (see § 6) with

this interface. Buttons called “High / Plus” and “Low / Less” are used to

navigate through the menu and to adjust parameters. When used for naviga-

tion, the “High / Plus” and “Low / Less” buttons can be used to move the

selection cursor. The same buttons also allow the adjustment of the homog-

enisation parameters.

NOTE:

The green light can have 3

different states: on, ﬂashing

or off.

The red light can have 3 differ-

ent states: on, ﬂashing or off.

The operator is responsible for programming the unit. For each speed range,

the manufacturer recommends an operating range to prevent abnormal tem-

perature rise. See § 6.3 for further details.

For high speed operation, it is recommended to reduce each cycle duration

and let the unit cool down between 2 cycles (at least 2 minutes). A security

device disables the unit to prevent overheating.

2.5 Presentation of the Keypad

The LCD screen lights up when the Precellys®24/ Precellys®24-Dual equip-

ment is switched on. These messages guide you for programming and con-

trolling the cycles. The keypad (see diagram below) consists of a LCD screen,

4 buttons and 2 lights (green, red).

Precellys®24 &Precellys®24-Dual

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USER MANUAL

230 V – 50 Hz (Europe standard)

110 V – 60 Hz (US standard)

<1 kVA

5x20 – T 10 A – H 250 V

Class A apparatus

290 mm

375 mm

370 mm

29,8 kg

15-30°C

15-85 % HR

< 2000 m

5000 – 6800 rpm 5000 – 6500 rpm

1 – 3

5 – 90 s

5 – 120 s

2 – 5 min

< 4 s

4 buttons

A 2 line LCD screen, 16 characters, back-light

2 lights (1 green, 1 red)

2 mL

1.4 mL

2.6 Technical Features

DESCRIPTION

Technical characteristics

Power requirements

Power consumption

Fuse speciﬁcations

Safety

Length

Width

Height

Weight

Operating temperature

Humidity

Altitude

Number of pre-registered

programs

Speed

Number of cycles

Cycle duration

Waiting time between 2 cycles

Rest time between 2

consecutive runs

Acceleration time

Deceleration time

Keypad

Display

Number of tubes

Total volume

Sample volume

12 / 6

2 mL / 7 mL

1.4 mL / 5 mL

Physical / Environmental conditions

Operating characteristics

User interface

Capacity

600 mm cover open

Modiﬁable programs

See adjustment limit § 6.3

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