Beckman Coulter Optima L-XP Manuel utilisateur

PN LXP-IM-7AB

October 2013

Instructions For Use

Optima L-XP Series

Preparative Ultracentrifuges

Beckman Coulter, Inc.

250 S. Kraemer Blvd.

Brea, CA 92821 U.S.A.

Optima L-XP Series

Preparative Ultracentrifuges

PN LXP-IM-7AB (October 2013)

No part of this document may be reproduced or

transmitted in any form or by any means,

electronic, mechanical, photocopying, recording, or

otherwise, without prior written permission from

Beckman Coulter, Inc.

Beckman Coulter, the stylized logo, and Quick-Seal are

trademarks of Beckman Coulter, Inc. and are registered in

the USPTO.

All other trademarks, service marks, products, or services

are trademarks or registered trademarks of their

respective holders.

Find us on the World Wide Web at:

www.beckmancoulter.com

Made in U.S.A.

Printed in U.S.A.

PN LXP-IM-7AB

iii

Safety Notice

Read all product manuals and consult with Beckman Coulter-trained personnel before attempting

to operate the instrument. Do not attempt to perform any procedure before carefully reading all

instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as

to how to proceed in any situation, contact your Beckman Coulter Representative.

Alerts for Warning, Caution, Important, and Note

WARNING

WARNING indicates a potentially hazardous situation which, if not avoided,

could result in death or serious injury.

CAUTION

CAUTION indicates a potentially hazardous situation, which, if not avoided,

may result in minor or moderate injury. It may also be used to alert against unsafe

practices.

IMPORTANT

IMPORTANT is used for comments that add value to the step or procedure being performed.

Following the advice in the Important adds benefit to the performance of a piece of equipment or to a

process.

NOTE

NOTE is used to call attention to notable information that should be followed during installation, use,

or servicing of this equipment.

Safety During Installation and/or Maintenance

This instrument is designed to be installed by a Beckman Coulter Field Service representative.

Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty

covering the instrument. Also, if the instrument needs to be moved, a Beckman Coulter Field

Service representative must reinstall and relevel the instrument in its new location.

Any servicing of this equipment that requires removal of any covers can expose parts which involve

the risk of electric shock or personal injury. Make sure that the power switch is turned off and the

instrument is disconnected from the main power source by removing the Mains (power) plug from

the outlet receptacle, and refer such servicing to qualified personnel.

Do not replace any ultracentrifuge components with parts not specified for use on this instrument.

PN LXP-IM-7AB

Safety Notice

Electrical Safety

To reduce the risk of electrical shock, this instrument uses a three-wire electrical cord and plug to

connect this equipment to earth-ground. Make sure that the matching wall outlet receptacle is

property wired and earth-grounded.

Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get into

the instrument and damage electrical or mechanical components.

Safety Against Risk of Fire

Fuses protect certain electrical circuits within this instrument against overcurrent conditions. For

conditioned protection against the risk of fire, replace only with the same type and rating specified.

This instrument is not designed for use with materials capable of developing flammable or

explosive vapors or hazardous chemical reactions. Do not centrifuge such materials (for example,

chloroform or ethyl alcohol) in this instrument nor handle or store them near the centrifuge.

Mechanical Safety

For safe operation of the equipment, observe the following:

• Use only the Beckman Coulter rotors and accessories designed for use in this instrument.

• Do not exceed the maximum rated speed of the rotor in use.

• NEVER attempt to slow or stop a rotor by hand.

• Do not move the ultracentrifuge while the drive motor is spinning.

• In the event of a power failure, do not attempt to retrieve the sample from the instrument for

at least one hour. Then follow the instructions for recovery of the sample under CHAPTER 5,

Troubleshooting.

Chemical and Biological Safety

Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or

radioactive. Such materials should not be used in this instrument unless all necessary safety

precautions are taken.

PN LXP-IM-7AB

Safety Notice

Chemical and Biological Safety

WARNING

Normal operation may involve the use of solutions and test samples that are

pathogenic, toxic, or radioactive. Handle body fluids with care because they can

transmit disease. No known test offers complete assurance that they are free of

micro-organisms. Some of the most virulent—Hepatitis (B and C) and HIV (I–V)

viruses, atypical mycobacteria, and certain systemic fungi—further emphasize the

need for aerosol protection. Handle other infectious samples according to good

laboratory procedures and methods to prevent spread of disease. Because spills

may generate aerosols, observe proper safety precautions for aerosol

containment. Do not run toxic, pathogenic, or radioactive materials in this

ultracentrifuge without taking appropriate safety precautions. Biosafe

containment should be used when Risk Group II materials (as identified in the

World Health Organization Laboratory Biosafety Manual are handled; materials of

a higher group require more than one level of protection.

WARNING

Do not use the ultracentrifuge in the vicinity of flammable liquids or vapors, and

do not run such materials in the instrument. Do not lean on the instrument or

place items on it while it is operating.

It is your responsibility to decontaminate the instrument and accessories before requesting service

by Beckman Coulter Field Service.

PN LXP-IM-7AB

Safety Notice

Chemical and Biological Safety

vii

Contents

Safety Notice,iii

Alerts for Warning, Caution, Important, and Note,iii

Safety During Installation and/or Maintenance,iii

Electrical Safety,iv

Safety Against Risk of Fire,iv

Mechanical Safety,iv

Chemical and Biological Safety,iv

Introduction,xv

Certification,xv

Scope of Manual,xv

CFC-Free Centrifugation,xvi

Recycling Label,xvi

RoHS Notice,xvi

Software Copyright,xvii

CHAPTER 1: Description, 1-1

Introduction,1-1

Instrument Function,1-1

Safety Features,1-2

Door,1-2

Barrier Ring,1-2

Imbalance Detector,1-2

Overspeed System,1-2

Dynamic Rotor Inertia Check (DRIC),1-3

Instrument Chassis,1-3

Rotor Chamber,1-3

Vacuum System,1-3

Drive,1-3

Temperature Sensing and Control,1-4

viii

Contents

Name Rating Plate,1-4

Controls and Indicators,1-4

Power,1-4

Key Switch Positions,1-5

Control Panel,1-5

Specifications,1-9

Available Rotors,1-10

CHAPTER 2: Operation,2-1

Introduction,2-1

Power,2-1

Key Switch Positions,2-1

Normal Position,2-2

Locked Position,2-2

Zonal Position,2-2

Touchscreen Interface,2-2

Basic Operation,2-3

Logging In,2-4

System-Level Operations,2-5

Adding a User,2-6

Copying Users,2-8

Set PIN Number,2-9

Set Role,2-10

Removing Users,2-10

Require User Login,2-11

Required Rotor Selection,2-11

Enable Run Comment,2-12

Manual Operation,2-12

Programmed Operation,2-15

Step Program,2-17

RPM to RCF Toggle,2-19

RCF Conversion,2-19

w2t,2-20

Rotor Conversion,2-22

Auto Pelleting,2-23

Auto Rate Zonal,2-24

Recall Program,2-26

Remote Operation,2-26

Using Calculation Features,2-27

Pelleting Time Calculation,2-27

Concentration Measures,2-28

Refractive Index/Density,2-29

Speed Reduction for Dense Solutions,2-29

Contents

Speed Reduction for Precipitating Solutions,2-30

Sedimentation Coefficient from Run Data,2-31

Sedimentation Coefficient From Molecular Mass,2-32

Restore Windows,2-33

CHAPTER 3: Simulations,3-1

Using Simulation Features,3-1

ESP Pelleting,3-1

ESP Rate Zonal,3-3

ESP Plasmid Run,3-4

ESP RNA (BEST),3-6

ESP RNA (Fast),3-7

Recall Simulation,3-9

CHAPTER 4: Reference and Log Functions,4-1

Reference Features,4-1

Rotor Catalog,4-1

Rotor Library,4-3

Tube Catalog,4-5

Chemical Resistances,4-6

Exporting Data,4-8

Log Features,4-10

Instrument Usage Log,4-10

Display Last Run Record,4-10

CHAPTER 5: Troubleshooting,5-1

Introduction,5-1

User Messages,5-1

Retrieving Your Sample in Case of Power Failure,5-3

CHAPTER 6: Care and Maintenance,6-1

Introduction,6-1

Cleaning,6-1

Rotor Chamber,6-1

Decontamination,6-2

Sterilization and Disinfection,6-2

Storage and Transportation,6-2

Supply List,6-3

Replacement Parts,6-3

Supplies,6-3

Contents

APPENDIX A: Preinstallation Requirements,A-1

Introduction,A-1

Space Requirements,A-1

Bio-Safety Level 3 Installation,A-2

Electrical Requirements,A-2

Attaching a Printer,A-3

APPENDIX B: Remote Access,B-1

Introduction,B-1

VNC License Information,B-2

GNU General Public License, Version 2, June 1991,B-3

Preamble,B-3

GNU General Public License Terms and Conditions for Copying,

Distribution and Modification,B-4

Beckman Coulter, Inc.

Optima L, LE, L-XP, and XL

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