EF600 Instruction Manual
Asymptote Ltd.
St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK
Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk
1. INTRODUCTION ................................................................................................5
1.1 WARNING..............................................................................................................6
1.2 SAFETY PRECAUTIONS...........................................................................................6
1.3 MAINTENANCE.......................................................................................................6
2. INSTALLING THE FREEZER UNIT ...............................................................7
2.1 UNPACKING ...........................................................................................................7
2.2 POSITIONING THE FREEZER....................................................................................7
2.3 ELECTRICAL CONNECTION ....................................................................................7
2.4 SWITCHING THE FREEZER ON .................................................................................7
4. USER INTERFACE (EF600-01, EF600-10).....................................................10
4.1 SELECTING THE LOGGING INTERVAL (EF600-01, EF600-10) AND FREEZING
PROFILE (EF600-10) ............................................................................................10
4.2 LOG FILE NAMES (EF600-01, EF600-10).............................................................11
5. OPERATING PROCEDURE FOR THE EF600 FREEZER .........................13
5.1 PRE-OPERATION CHECKS......................................................................................13
5.2 PREPARING THE NUCLEATING TOOL .....................................................................13
5.3 PREPARING THE STRAWS......................................................................................13
5.4 LOADING THE STRAWS ........................................................................................13
5.5 STARTING THE DATA LOGGING (EF600-01, EF600-10) .......................................14
5.6 STARTING THE FREEZING CYCLE ..........................................................................14
5.7 SAMPLE NUCLEATION..........................................................................................15
5.8 END OF CYCLE .....................................................................................................15
5.9 STOPPING THE DATA LOGGING .............................................................................16
5.10 RESTARTING THE CYCLE ......................................................................................16
5.11 CLEANING THE UNIT ............................................................................................16
6. FREEZING CYCLES ........................................................................................17
6.1 HUMAN EMBRYO CRYOPRESERVATION ................................................................17
6.2 HUMAN OOCYTE CRYOPRESERVATION .................................................................17
6.3 HUMAN BLASTOCYST CRYOPRESERVATION .........................................................18
6.4 HUMAN EMBRYONIC STEM CELL CRYOPRESERVATION.........................................18
6.5 HUMAN SPERMATOZOA CRYOPRESERVATION –LINEAR COOLING ........................19
6.6 HUMAN SPERMATOZOA CRYOPRESERVATION –NON-LINEAR COOLING ................20
7. REFERENCES....................................................................................................21
