Artel PCS3 Manuel

15A3356O
PCS®3
Procedure Guide
®
25 Bradley Drive
Westbrook, Maine USA 04092-2013
tel: 207-854-0860, toll free: 1-888-406-3463
fax: 207-854-0867
email: [email protected]
email: [email protected]
web: www.artel-usa.com

15A3356O
Note: This Guide applies to PCS®instruments with firmware
versions 7A2.XXX or 7A3.XXX, where “XXX” represents a three
digit number between 001 and 999. Changes for later versions
may be described in addenda and inserted following this page.
Document Number & Revision: 15A3356O
Document Revision Date: June 2017
Copyright © 2017 Artel, Inc.
Products are intended for use with the Artel PCS as applicable.
The Artel PCS and its components are covered by patents listed at www.artel-
usa.com/patents.
All Artel product Safety Data Sheets are available at www.artel-usa.com/support-
ordering.
All rights reserved. No part of this publication may be reproduced, stored in a
retrieval system, or transmitted, in any form or by any means, electronic,
mechanical, photocopying, recording, or otherwise, without the prior written
permission of the publisher. Printed in the United States of America.
Artel, the Artel logo and PCS are registered trademarks of Artel, Inc., Westbrook,
Maine.
Artel
25 Bradley Drive
Westbrook, ME 04092-2013
U.S.A.

15A3356O
TABLE OF CONTENTS
(Sections 1-5)
1.0 INTRODUCTION ……………………………………………………………………….1
2.0 INSTRUMENT PREPARATION
2.1 Location ..................................................................................................... 3
2.2 Set-up and Cabling Illustration ................................................................... 3
2.3 Cabling Procedures ................................................................................... 4
2.4 Seiko Printer .............................................................................................. 4
2.5 RS232 Port ................................................................................................ 5
3.0 REAGENT KIT
3.1 Contents of Standard Reagent Kit ............................................................. 7
3.2 Cleaning of PCS Vials ................................................................................ 8
3.3 Storage ...................................................................................................... 8
3.4 Disposal ..................................................................................................... 8
3.5 Ordering ..................................................................................................... 8
4.0 OPERATION
4.1 Instrument Keys and Operating Functions ................................................. 9
4.2 Initial Power-On Sequence ...................................................................... 10
4.3 Starting Pipette Calibration ...................................................................... 10
4.4 Inserting the Blank Vial ............................................................................ 13
4.5 Entering Pipette ID Number ..................................................................... 14
4.6 Entering Pipette Volume .......................................................................... 14
4.7 Pipetting Sample Solution ........................................................................ 15
4.8 Pipetting Additional Samples ................................................................... 17
4.9 Eliminating Sample Readings and Reprinting Results ............................. 17
4.10 Performing Additional Calibrations In One Vial ........................................ 18
4.11 Continuing a Calibration Run with Another Vial ....................................... 20
4.12 Calibrating an Adjustable Volume Pipette at Multiple Volumes ................ 20
4.13 Suggested Initial Tolerance Limits ........................................................... 21
5.0 INSTRUMENT CAL A AND CALIBRATION
5.1 Instrument CAL A .................................................................................... 23
5.2 Instrument Calibration ............................................................................. 23
5.3 Instrument Calibration Summary ............................................................. 27

15A3356O
TABLE OF CONTENTS
(Sections 6-App. F)
6.0 INSTRUMENT SETUP
6.1 Instrument Setup Menu............................................................................ 29
6.2 Setting Date ............................................................................................. 30
6.3 Setting Time ............................................................................................. 30
6.4 Choosing Date Format ............................................................................. 31
6.5 Customizing the Printout Header ............................................................. 31
6.6 Computer Link Flags ................................................................................ 32
6.7 Serial Data Output (Data Collect Flag) ..................................................... 33
6.8 Choose Nomenclature ............................................................................. 34
6.9 Display Uncertainty .................................................................................. 35
6.10 Signature Block ........................................................................................ 36
7.0 TROUBLESHOOTING
7.1 Instrument Diagnostics Menu .................................................................. 37
7.2 Troubleshooting Guide ............................................................................. 40
8.0 SYSTEM MAINTENANCE
8.1 Preventive Maintenance .......................................................................... 45
8.2 Instrument Calibration .............................................................................. 45
8.3 Removal of Rear Access Cover ............................................................... 45
8.4 Maintenance Log Sheet ........................................................................... 46
9.0 WARRANTY AND SERVICE
9.1 Warranty Statement ................................................................................. 47
9.2 Return and Repair ................................................................................... 47
APPENDICES
Specifications ....................................................................................................... A
PCS Printouts ....................................................................................................... B
Formulas Used to Calculate Group Statistics ....................................................... C
ASCII Character Set ............................................................................................. D
Alphabetic Character Entry ................................................................................... E
Number of Samples Per Vial ................................................................................ F
Consumables ....................................................................................................... G

15A3356O 1
1.0 INTRODUCTION
Accuracy and precision in volumetric measurement and transfer are essential to
all laboratories concerned with maintaining quality and productivity. Pipette
performance can vary significantly over time, and conformance to specified
accuracy and precision levels cannot be taken for granted.
Employing a colorimetric method and a high performance photometer, the PCS®
allows the user to accurately, precisely, quickly, and easily measure the
dispensed volume from any pipette (from 0.1 μL to 5000 μL). Measurements
made with the Artel Pipette Calibration System (PCS®) are traceable to the
International System of Units (SI) through reference standards developed and
maintained by the National Institute of Standards and Technology, USA (NIST).
The volume of liquid delivered is immediately calculated and displayed for each
data point. A Printout is generated displaying the volume of each sample and the
group statistics. The Printout includes such information as pipette ID, operator ID,
time, temperature, and date to assist with effective record keeping.
Additionally, by providing immediate feedback to the user, the PCS is an effective
tool for improving pipetting technique.
With a minimal amount of training an operator can calibrate pipettes both when
and where needed, improving workflow, quality, and efficiency in the laboratory.
This manual provides step-by-step instructions for all major operations to assist
the user to be up and running more quickly.

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2.0 INSTRUMENT PREPARATION
2.1 Location
The Instrument should be located in an area that is out of direct sunlight
and away from local heat sources (e.g. an oven or heat vent).
2.2 Set-up and Cabling Illustration

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2.3 Cabling Procedures
2.3.1 Connecting the Instrument to Power
The Instrument requires a three-prong electrical receptacle
supplying single-phase power from 100-250 VAC, 50/60 Hz in
which to plug the Power Supply. The same power source should be
used for the Instrument and the Printer. This power line should be
free from transients or voltage variations caused by other
equipment such as water baths or refrigerators.
Make sure that the Instrument Power Switch is off. Plug one end of
the Power Cord into the Power Supply and the other end of the
Power Cord into a grounded AC outlet. Insert the remaining plug
into the power receptacle found at the rear of the Instrument.
IMPORTANT Ensure that all above connections are firmly in place.
2.3.2 Connecting the Seiko Printer to Power
NOTE: If the STAR Dot Matrix Printer is being used, please refer to the STAR Printer
User’s Manual for printer connection and operation.
Plug the Printer AC adapter into an appropriate receptacle.
Plug the remaining end of the adapter into the power receptacle
located at the rear of the Printer.
2.3.3 Connecting the Printer to the Instrument
Make sure the Instrument and Printer are turned off. Plug one end
of the Printer cable into the Printer and the other into the
Instrument.
IMPORTANT When the Instrument and Printer are connected, the Instrument must be powered on to
operate the Printer.
2.4 Seiko Printer
2.4.1 Controls and Indicators:
Power Switch. When slid to the ON position, power is supplied to
the Printer and the POWER and ON LINE indicators illuminate.
When there is no paper loaded, the OFF LINE indicator flashes.

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Paper Feed Switch. Pressing this switch feeds the paper
continuously. Paper feed will work only when the OFF LINE
indicator illuminates or flashes.
On Line/Off Line/Paper End Switch. Pressing this switch shifts
the Printer from ON LINE to OFF LINE and vice versa. When ON
LINE, the Printer is ready to receive instructions from the
Instrument. The Printer cannot be turned ON LINE if paper is not
loaded.
2.4.2 Loading Printer Paper
Turn the Power Switch to the ON position.
Open the paper cover located on the top right hand side of the
Printer.
Cut the end of the paper roll cleanly and squarely.
Insert the end of the paper into the inlet at the bottom of the paper
holder until the auto-loader catches it and feeds it through. The
outside of the roll is the printable surface for the thermal paper roll.
Close the paper cover.
NOTE: A red line will appear on both sides of the paper when the paper roll nears
depletion. This indicates that the paper roll should be changed.
2.4.3 Preparing the Printer for Pipette Calibration
Once the paper roll is in place, the Instrument and the Printer can
be turned ON. When the ON LINE light is green, the printer is ready
for operation.
NOTE: Refer to the printer operation manual for more detailed information.
2.5 RS232 Port
Refer to Section 6.7, “Serial Data Output,” for information on use of the
RS232 port.

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