AND BM Series Manuel utilisateur

Pipette Accuracy Tester Kit

for the BM series

BM-014

INSTRUCTION MANUAL

1WMPD4002534

CONTENTS

1. INTRODUCTION............................................................................................................................... 2

1-1 Unpacking the Tester................................................................................................................... 2

1-2 Description of Each Component .................................................................................................3

1-3 WinCT-Pipette, Software for Pipette Accuracy Testing ............................................................... 4

1-4 Function of the Evaporation Trap................................................................................................ 5

1-5 Description of the 5 mL Sample Cup........................................................................................... 5

2. ASSEMBLING THE TESTER........................................................................................................... 6

3. PRECAUTIONS BEFORE USE........................................................................................................ 8

4. ANTI-STATIC TREATMENT ............................................................................................................. 9

5. INSTALLING WinCT-Pipette............................................................................................................ 9

6. CONNECTING A PC TO THE BALANCE...................................................................................... 10

6-1 Connecting a PC ....................................................................................................................... 10

6-2 Checking COM Port Numbers....................................................................................................11

7. CALIBRATING THE BALANCE..................................................................................................... 12

7-1 Calibration using internal weight (Calibration of balance)......................................................... 12

8. PREPARATION BEFORE USE...................................................................................................... 13

8-1 Filling the Evaporation Trap with Water .................................................................................... 13

8-2 Preparing the Test Liquid........................................................................................................... 13

8-3 Preparing the Water-absorbent Sheet....................................................................................... 13

8-4 Using the Thermometer............................................................................................................. 14

9. MEASUREMENT ............................................................................................................................ 18

9-1 Measuring Procedure................................................................................................................ 18

9-2 Judging the Results................................................................................................................... 18

9-3 Example Causes of Measurement Errors................................................................................. 18

10. FUNCTION SETTING OF THE BALANCE.................................................................................. 20

10-1 Display Lock Function............................................................................................................. 20

10-2 Auto Print Mode....................................................................................................................... 20

11. TROUBLESHOOTING.................................................................................................................. 21

12. OPTIONS ...................................................................................................................................... 22

13. APPENDIX .................................................................................................................................... 23

1. INTRODUCTION

The Pipette Accuracy Tester Kit for the BM series performs an accuracy test of the volume of a

pipette in accordance with ISO 8655.

This manual describes how the Pipette Accuracy Tester Kit works and how to get the most out of it in

terms of performance. It includes how to assemble the tester, how to measure and how to use the

software, WinCT-Pipette, for pipette accuracy testing.

Read this manual thoroughly before using the tester and keep it at hand for future reference.

For detailed information on handling the balance, refer to the instruction manual for the balance used.

NOTE ISO 8655 is the international standard that includes pipette calibration and specifies a test

method for accuracy of the pipette volume. For detailed information, visit our web site.

1-1 Unpacking the Tester

NOTE The pipette accuracy tester kit is contained in a carrying case. The case is only for

carrying the tester and is not solid enough to endure transportation. So, do not

transport the tester using the carrying case. When transporting the tester, be sure to

use the original packing material.

Whenhandling the tester, be careful notto drop it, even if it is placed in the carrying case.

The illustration below shows the content of this kit.

Note that the USB converter is shipped in a separate package, but it can be placed in the carrying

case as shown above.

Cap A

Cap B Evaporation trap

USB converter

Carrying

case

Thermometer

clamp

Test liquid cup

RS-232C cable

(9P-9P)

Instruction manual

Pipette accuracy

tester kit for the

BM series

Sample cup holder

for BM

Evaporation trap

base

WinCT-Pipette

(CD-ROM)

(water-absorbent sheet

is provided inside)

Blance weight

USB converter

driver

(CD-ROM)

5 mL sample cup (2 pcs)

Center

positioning

boss

1-2 Description of Each Component

Evaporation trap

A chamber to prevent the evaporation of the dispensed test liquid (distilled water).

(Refer to “1-4 Function of the Evaporation Trap”.)

Sample cup holder for BM

Use to hold the 5 mL sample cup.

5 mL sample cup

A measuring cup to contain the test liquid dispensed from a pipette, with a

water-absorbent sheet installed, to prevent water droplets from remaining on

the edge of the pipette tip.

Test liquid cup

A container to keep a test liquid such as distilled water.

Thermometer (with a clamp)

Measures the temperature of the test liquid.

RS-232C cable and USB converter

Connects to a PC (personal computer) to transmit the test

values to the PC.

WinCT-Pipette

Software for pipette accuracy testing to convert the test liquid mass

into a volumetric value.

Balance weight

Use this balance weight when using the pipette accuracy tester kit with

the following BM models: BM-252/500/300/200.

1-3 WinCT-Pipette, Software for Pipette Accuracy Testing

Features

The mass value of the distilled water dispensed from a pipette is transmitted from the balance to a

PC. Using the temperature of the test liquid and the barometric pressure that are previously entered,

the PC calculates the conversion factor (Z factor) from a mass value to a volumetric value and

converts the mass value into a volumetric value automatically, using the Z factor.

The measurement results are compared with the specifications previously entered, to perform

judgment whether the pipette will pass or fail.

Besides the pipette volume, test numbers, test dates and pipette information (manufacturer, model,

serial number) can be recorded as test data. The test data, output to a printer or stored in the PC,

allows easymanagementof pipette accuracy and reduces errors due to data mismanagement.

The specifications of accuracy and repeatability can be entered either as an absolute value (L)

or as a relative value (percentage to the pipette volume).

The mass value data from the balance can be transmitted to the PC either using a COM port or

using a USB connection. (For a USB connection, use the accessory USB converter.)

For testing various pipettes easily, multiple test conditions (pipette volume, number of

measurements and specifications) can be set previously. When multiple operators are set

previously, an operator can be selected easily as necessary.

NOTE Fordetailed information onWinCT-Pipette, refer to “How to use WinCT-Pipette.pdf”in the

WinCT-Pipette CD-ROM.

Main Window

When WinCT-Pipette starts up, the window below opens

Specifications

Enterthepipettevolume,specificationsof

accuracy and repeatability used for

pass/fail judgment.

Measured Values

Displays the mass values transmitted from the

balance and the volumetric values obtained

using the Z factor.

Measurement Results

Displays the measurement results and

judgment results.

The Z factor is automatically calculated using the values of

thedistilledwatertemperature andthe barometricpressure.

Measurement environment

Entertheambientrelativehumidity,

thedistilledwatertemperatureand

the barometricpressure.

1-4 Function of the Evaporation Trap

In the pipette accuracy test using the gravimetric method, to obtain the mass of the test liquid, distilled

water is dispensed from the pipette into the sample cup installed on the balance. The obtained value,

however, is generally smaller due to evaporation loss of the test liquid. To prevent evaporation of the test

liquid, the evaporation trap is used. The ISO 8655 standard recommends that the humidity of the

measurement environment be greater than 50% RH.

Water is poured into the groove of the evaporation trap. This helps to maintain the humidity inside

the evaporation trap high and reduces evaporation. Using the evaporation trap reduces the

evaporation of the test liquid, compared with the value when the evaporation trap is not used. This

yields a measurement environment where there is no need to consider measurement errors due to

evaporation loss of the test liquid.

Another function of the evaporation trap is one as a breeze break, to prevent drafts in the

measurement environment from influencing the weighing operation and enable a stable weighing.

The evaporation trap is designed for an easy dispensing of the test liquid. The upper part has a

sloped surface so that the pipette can be inserted from obliquely above.

1-5 Description of the 5 mL Sample Cup

When dispensing a test liquid from the pipette, to perform an accuracy test of the volume of a pipette, a

possible cause of errors is water droplets remaining on the edge of the pipette tip. Using the

water-absorbent sheet in the sample cup prevents this and increases the accuracy of a test.

As shown in the illustration below, a water-absorbent sheet is rolled into a cylinder and is placed

along the inner wall of the sample cup.

By dispensing the test liquid with the edge of the pipette tip touching the sheet, the sheet will absorb

any droplet which may otherwise remain on the edge of the tip. Thus all the test liquid will be

dispensed into the sample cup.

The water-absorbent sheets are made of a RoHS-compliant material for safety.

The sheet, once used, can be cleaned and used repetitively.

2. ASSEMBLING THE TESTER

Preparing to assemble the tester

Remove the separation plate from the weighing

chamber of the BM balance.

Note:Take care that the separation plate is

not broken when operating it.

1 Support the separation plate and

remove the screw from the plate.

2 Lift the plate and remove hooks.

3 Rotate the plate on the axis of the back edge.

Rotate the plate on the axis of the front edge.

4 Remove the plate from the chamber.

5Remove the weighing pan and so on (refer to the figure below) from the BM balance.

Hook

Screw

Hook

Separation plate

ＢＭ－２００､ＢＭ－３００､ＢＭ－２５２

ＢＭ－５００､ＢＭ－２５２

ＢＭ－２２､ＢＭ－２０

Weighing pan

Breeze break

ring

Dust plate

Pan support

Weighing pan

Small fine range ring

Dust plate

Fine range ring

Assembling the tester

1 Install the center positioning boss and evaporation trap base on the BM balance.

2Install the sample cup holder on the center positioning boss.. In the case of the

BM-252/500/300/200, install the balance weight on the sample cup holder for the BM balance.

3Install the 5 mL sample cup on the sample cup holder.

4 Install the lower part of the evaporation trap on the evaporation trap base.

5 Install the upper part of the evaporation trap on the lower part to complete assembly.

Completed

Centerpositioning

boss

Evaporation

trap base

Samplecup

holderforBM

5 mL sample cup

Balance weight

BM-252/500/300/200

Evaporation trap

(Upper part)

Evaporation trap

(lower part)

3.PRECAUTIONS BEFORE USE

Arrange the following setup conditions to prevent the negative effects of temperature change,

vibration, or breezes and ensure stable balance performance.

The BM series, especially the BM-20/22, has a high weighing sensitivity with a minimum weighing

value of 0.001 mg, so it is important to give proper attention to the setup conditions before using these

balances.

Install the balance where the measurement environment is appropriate. The best operating

temperature is 15°C to 30°C ±0.5°C with greater than 50% RH.

Install the balance where it is not exposed to direct sunlight and it is not affected by air from

heaters or air conditioners.

Install the balance where it is free of dust.

Install the balance away from equipment which produces magnetic fields.

Install the balance in a stable place avoiding vibration and shock. Corners of rooms (close to

pillars or walls) far from a passage way are best, as they are less prone to vibration.

The weighing table should be solid and free from vibration, drafts and as level as possible.

Level the balance by adjusting the leveling feet and confirm it using the bubble spirit level.

Before use, warm up the balance for at least one hour with nothing on the weighing pan.

Calibrate the balance before use or after having moved it to another location. For details, refer

to “7. CALIBRATING THE BALANCE”.

Caution

Do not install the balance where flammable or corrosive gas is present.

4. ANTI-STATIC TREATMENT

An anti-static treatment has been applied to the evaporation trap. Cleaning it with alcohol will reduce

the effect of the treatment.

If the evaporation trap has acquired a static charge, we recommend that you apply an anti-static

agent or use the built-in static eliminator of the BM series to remove the static electricity.

5. INSTALLING WinCT-Pipette

For detailed information on the WinCT-Pipette program, refer to “How to use WinCT-Pipette.pdf”in

the WinCT-Pipette CD-ROM.

For installing WinCT-Pipette, refer to “Readme.txt”in the WinCT-Pipette CD-ROM.

System requirements

Windows XP SP2 or later

CPU

Pentium or the equivalent, 1GHz or more recommended

RAM

512 MB or more recommended

Hard disk available space

Approximately 50 MB

Display

1024 x 768 or more recommended

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