AKTA design UV-900 Manuel utilisateur
User Manual
18-1120-05
Monitor UV-900
18-1120-05_AB.qxd 1998-10-22 18:01 Sida 1 (1,1)
Trademarks
ÄKTA and UNICORN are trademarks of Amersham
Pharmacia Biotech Limited or its subsidiaries.
Amersham is a trademark of Nycomed Amersham plc.
Pharmacia and Drop Design are trademarks of
Pharmacia & Upjohn Inc.
Terms and Conditions of Sale
All goods and services are sold subject to the terms
and conditions of sale of the company within the
Amersham Pharmacia Biotech group which supplies
them. A copy of these terms and conditions of sale is
available on request.
Adresses
Amersham Pharmacia Biotech UK Limited
Amersham Place
Little Chalfont
Buckinghamshire
England HP7 9NA
Amersham Pharmacia Biotech AB
SE-751 84 Uppsala
Sweden
Amersham Pharmacia Biotech Inc
800 Centennial Avenue
PO Box 1327
Piscataway
NJ 08855 USA
© Amersham Pharmacia Biotech AB 1998
– All rights reserved
Important user information
Meaning: Consult the instruction manual to avoid
personal injury or damage to the product or other
equipment.
WARNING!
The Warning sign is used to call attention to the
necessity to follow an instruction in detail to avoid
personal injury. Be sure not to proceed until the
instructions are clearly understood and all stated
conditions are met.
CAUTION!
The Caution sign is used to call attention to
instructions or conditions that shall be followed to
avoid damage to the product or other equipment. Be
sure not to proceed until the instructions are clearly
understood and all stated conditions are met.
Note
The Note sign is used to indicate information
important for trouble-free or optimal use of the
product.
Should you have any comments on this instruction, we
will be pleased to receive them at:
Amersham Pharmacia Biotech AB
SE–751 84 Uppsala
Sweden
!
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Contents
1 Introduction
1.1 General ..................................................................................................................1
1.2 Safety.....................................................................................................................1
2 Installation
2.1 Unpacking..............................................................................................................2
2.2 General precautions ...............................................................................................2
2.3 Installing the flow cell............................................................................................2
2.4 Connecting electrical signal cables .........................................................................5
2.5 Connecting to communication link........................................................................5
2.6 Connecting to supply voltage.................................................................................6
2.7 Connecting the tubing............................................................................................6
2.8 Mounting the cell holder cover..............................................................................6
3 Operation
3.1 On/off....................................................................................................................7
3.2 Menu selection and settings ...................................................................................7
3.3 Main menu overview .............................................................................................8
3.4 Setting lamp on/off ................................................................................................9
3.5 Reading absorbance value......................................................................................9
3.6 Setting wavelength .................................................................................................9
3.7 Autozero..............................................................................................................10
3.8 Storage and shut-down ........................................................................................10
3.9 Using an external chart recorder..........................................................................10
3.10 Filtering noise ......................................................................................................11
3.11 Changing flow cell...............................................................................................12
3.12 Restart after power failure...................................................................................12
4 Maintenance
4.1 Periodic maintenance ...........................................................................................13
4.2 Cleaning-in-place .................................................................................................13
4.3 Checking the instrument......................................................................................13
4.4 Cleaning the flow cell and optical fibre connectors..............................................14
4.5 Instrument housing ..............................................................................................14
5 Trouble shooting
5.1 General ................................................................................................................15
5.2 Faults and actions................................................................................................15
5.3 Error messages.....................................................................................................16
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User Manual 18-1120-05 Edition AB i
Contents
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Reference information
A Description ..........................................................................................................17
A.1 Instrument.................................................................................................17
A.2 Flow cell....................................................................................................18
A.3 Monitor principle......................................................................................18
B Menus..................................................................................................................20
B.1 Check menu ..............................................................................................20
B.1.1 Checking autozero level............................................................20
B.1.2 Lamp intensity, Lamp Ontime, Fliptime ...................................20
B.1.3 Checking the wavelength ..........................................................20
B.1.4 Recalibrate................................................................................20
B.1.5 Check Analogue Out ................................................................21
B.1.6 Check Service Mode .................................................................21
B.2 Setup menu ...............................................................................................21
B.2.1 Setup language..........................................................................21
B.2.2 Setup unit number ....................................................................22
B.2.3 Setup display angle ...................................................................22
B.3 Setting and using the alarm timer..............................................................22
B.4 Service displays .........................................................................................23
B.5 Menu overview .........................................................................................24
C Technical specifications .......................................................................................25
D Accessories and spare parts..................................................................................27
Short instructions on back page
About this manual
This manual comprises two parts; a practical part (sections 1 – 5)
and a reference part (sections A – D).
Sections 1 – 5 contain the necessary information for operating the
instrument.
Monitor UV-900
User Manual 18-1120-05 Edition AB
ii
Contents
18-1120-05_AB.qxd 1998-10-22 18:01 Sida ii (1,1)
1 Introduction
1.1 General
Monitor UV-900 is a UV-absorption monitor for use in liquid
chromatography. Monitor UV-900 features:
• Variable wavelength for detection in the range 190–700 nm.
• Up to 3 wavelengths can be monitored simultaneously.
• Two alternative flow cells with path length 2 mm and 10 mm.
• Accurate and reliable monitoring through self-test, self-calibration
and fibre optics.
1.2 Safety
• The instrument is designed for indoor use only.
• Do not use in a dusty atmosphere or close to spraying water.
Monitor UV-900
Monitor UV-900
User Manual 18-1120-05 Edition AB 1
WARNING! The instrument must not be opened by the user. It
contains high voltage circuits which can give a lethal electric shock.
WARNING! The instrument uses high intensity ultra-violet light.
Do not disconnect the optical fibres while the monitor is running.
WARNING! Always disconnect the power supply before
attempting to replace any item on the instrument.
WARNING! The instrument must be connected to a grounded
mains socket.
Introduction
1
WARNING! When using hazardous chemicals, all suitable
protective measures, such as protective glasses, must be taken.
WARNING! The flow cells must not be used at pressures above
2 MPa (20 bar, 290 psi). At higher pressures the flow cells may
break.
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2 Installation
2.1 Unpacking
Unpack the instrument and check the items against the supplied
packing list. Inspect the items for obvious damage which may have
occurred during transportation.
It is recommended that all packing materials should be retained if
onward transport of the instrument is expected.
2.2 General precautions
The instrument should not be installed in a corrosive atmosphere or
in an atmosphere where deposits are liable to form on the optical
surfaces.
The instrument should be located in a place of low temperature
variations, away from heat sources, draughts and direct sunlight.
The instrument may be operated at normal ambient temperatures in
the range +4 to +40 °C.
The instrument should be installed on a stable laboratory bench or
in ÄKTA™ explorer or ÄKTApurifier. To ensure correct ventilation a
free space of 0.1 m is required behind and in front of the instrument.
Place the instrument directly on the bench. Do not use any soft
material under the instrument, to ensure that the ventilation inlet in
the front is not blocked.
2.3 Installing the flow cell
Fixing the flow cell
Handle the optical fibres with care, do not bend excessively.
Two flow cells are available: UV-900/2 with 2 mm light path length
(cell volume 2 µl) and UV-900/10 with 10 mm path length (8 µl).
CAUTION! The following information should be read carefully
to ensure that the instrument is installed correctly.
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User Manual 18-1120-05 Edition AB
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2
Installation
UV-900
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1Unpack the flow cell. Do not remove the red protective caps from
the inlet or outlet, or the black protective caps on the optical
fibre connectors.
2 Slide the rear white clip on the cell holder to its inner position for
a 2 mm cell and to its outer position for the 10 mm cell.
3 Place the flow-cell in the opening between the white clips. The
cell should be positioned with the text and serial number facing
upwards and to the front. Press the cell into the clips to fasten it.
CAUTION! To avoid damaging the optical fibres, press only on
the cell body, never on the optical fibres.
To improve the access to the tubing connections, the flow cell can
alternatively be positioned with the tubing connections
horizontal.
Normal position Alternative position
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User Manual 18-1120-05 Edition AB 3
Installation
2
10 mm 2 mm
Serial
Number Serial
Number
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Connecting the optical fibres
1 Remove the 2 black protective caps from the optical fibre
connectors.
2 Remove the 2 rubber protective caps from the optical fibre
receptacles on the right side of the housing.
3 Connect the 2 optical fibres to the housing by carefully inserting
them into the sockets and tightening the nuts fingertight using the
fibre detachment tool supplied.
4 Slide the rubber sleeves on the 2 optical fibres onto the
connectors. Make sure that the sleeves are pushed tight to the
housing to prevent dust or fluid entering the connection.
5. To “run-in” the new optical fibres prior to its use in normal
operation, run the monitor for 8 hours at 215 nm.
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User Manual 18-1120-05 Edition AB
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2
Installation
CAUTION! Do not touch the tips of the optical fibres with
your fingers as this will result in poor monitor performance. If
you accidentally touch the optical fibre tip, it can be wiped
with 30% isopropanol using lens paper.
Rubber sleeve
Fibre
detachment
tool
CAUTION! Do not twist the optical fibres during tightening.
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2.4 Connecting electrical signal cables
The sockets for electrical signals are located on the back panel.
Connecting to recorder (if used)
1 Connect the recorder to the miniDIN-socket Analogue out using
the cable supplied. Each wavelength signal is available on a
separate channel using the following wires:
λ1Wire 1 +
Wire 2 –
λ2 Wire 3 +
Wire 4 –
λ3 Wire 5 +
Wire 6 –
Note: The signal cable is delivered with protective covers on each wire.
Do not remove the protective covers from unused connections as a
short circuit may disturb the measurements.
2 Set the recorder to 0–1 V input, full scale, 0 V offset.
2.5 Connecting to communication link
The monitor can be used in ÄKTAexplorer or ÄKTApurifier and
controlled from a PC running UNICORN™version 2.20 or higher,
using UniNet cables.
1 Connect two UniNet cables to the UniNet 1 connectors. The
instrument can be connected in series anywhere in the chain
between the PC and the termination plug. The UniNet 1 link
connects, in series, the PC with Pump P-900, Monitor pH/C-900,
Monitor UV-900 and Frac-900. The termination plug is
connected to the last instrument in the chain.
Analogue out
0-1V Remote UniNet 1 Mains On/off
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User Manual 18-1120-05 Edition AB 5
CAUTION! The mains power to ÄKTAexplorer or ÄKTApurifier
must be switched OFF before connecting the instrument to the
UniNet 1 link.
Installation
2
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2.6 Connecting to supply voltage
1Make sure the on/off switch is in the OFF-position (O).
2 Connect a mains cable between the instrument and a grounded
mains socket. The instrument is delivered with both European
and US type mains cables, as standard. Any voltage
100–240 V AC, 50–60 Hz can be used.
The instrument contains no user replaceable fuse.
2.7 Connecting the tubing
1Remove the red protective caps from the inlet and outlet of the
flow-cell and connect the tubing with 1/16" “Fingertights”.
The inlet is on the top of the flow cell, the same side as the serial
number.
2.8 Mounting the cell holder cover
The cover is a simple push fit onto the cell holder. Two small lugs
on the cover locate in holes at the front and rear of the cell holder
The cover is then lowered over the cell holder.
Inlet
Outlet
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2
Installation
WARNING! The instrument must be connected to a grounded
mains socket.
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