Affinité Instruments P4SPR Manuel utilisateur

www.affiniteinstruments.com Rev 2.0

Portable 4-Channel Surface

Plamson Resonance (P4SPR)

User Manual

Research Use Only

Revision History

October 13 2020: First version for production version of P4SPR

October 26 2020: Fixed Figure 37

November 20 2020: revised technical specifications

November 25 2020: correcte page numbers

December 09 2020: Updated driver install for Flame T

December 17 2020: updated some maximum rating conditions

iii 
 
Table of Contents 
GENERAL INFORMATION ............................................................................................................ 2 
Purpose of this manual..........................................................................................................................................2 
Device overview ........................................................................................................................................................2 
Before you start.....................................................................................................................................................2 
P4SPR Starter-Kit Checklist........................................................................................................................................2 
Unpacking instructions..............................................................................................................................................4 
TECHNICAL SPECIFICATIONS ....................................................................................................... 5 
System Specification..............................................................................................................................................5 
Microfluidic cell .....................................................................................................................................................6 
Analytical performances........................................................................................................................................6 
Limitations of liability ............................................................................................................................................6 
Safety Notes ..........................................................................................................................................................7 
Environmental requirements.................................................................................................................................7 
Chemical compatiblity ...........................................................................................................................................7 
INSTALLATION ............................................................................................................................ 9 
Instrument Installation ..........................................................................................................................................9 
SPR sensor insertion................................................................................................................................................10 
Tubing connection...................................................................................................................................................11 
Leak Test..................................................................................................................................................................12 
Software Installation ........................................................................................................................................... 12 
Installation troubleshooting....................................................................................................................................16 
OPERATION INSTRUCTIONS .......................................................................................................19 
General guidelines ............................................................................................................................................... 19 
Overall setup of the P4SPR .................................................................................................................................. 20 
Sensor set up...........................................................................................................................................................20 
Microfluidic setup ...................................................................................................................................................21 
SOFTWARE AND GUI..................................................................................................................22 

iv 
 
Overview of P4SPR data acquisition ....................................................................................................................22 
Software and device setup................................................................................................................................... 23 
Graphical User Interface (GUI) Quick Start........................................................................................................... 24 
GUI Tabs .............................................................................................................................................................. 27 
Data Acquisition Tab ...............................................................................................................................................27 
Spectroscopy Tab ....................................................................................................................................................28 
Parameter Tab.........................................................................................................................................................29 
Additional GUI Hints ............................................................................................................................................30 
Graphic display........................................................................................................................................................30 
Cursor control .........................................................................................................................................................31 
Graphic and Channel Display –Full Sensorgram View............................................................................................32 
Graphic and Channel Display –Segment of Interest...............................................................................................33 
Segments table........................................................................................................................................................34 
Display Channel Average on Segment of Interest graph.........................................................................................35 
Activate reference...................................................................................................................................................35 
Data Processing Tab ................................................................................................................................................36 
Graphics Tab............................................................................................................................................................37 
GUI Example: Determining of SPR shift of different solutions..............................................................................38 
Export data..............................................................................................................................................................39 
Cleaning procedure and store device......................................................................................................................39 
DATA OUTPUT AND ANALYSIS....................................................................................................40 
Processed Data Representation........................................................................................................................... 40 
Structure in saved folder .....................................................................................................................................41 
Data processing of Table in Excel (case of calibration curve with NaCl in water).................................................. 41 
TROUBLESHOOTING ..................................................................................................................43 
Setting up a microfluidic cell................................................................................................................................43 
Troubleshooting via Spectroscopy Tab ................................................................................................................ 43 
Out of range refractive index ..................................................................................................................................43 
Air bubble................................................................................................................................................................44 
Leak in sample holder .............................................................................................................................................46 
Saturating signal......................................................................................................................................................47 
Misplaced sample cell holder..................................................................................................................................48 
Sensor transmittance above T20%..........................................................................................................................48 
MAINTENANCE..........................................................................................................................49 

Rinsing before and after each run........................................................................................................................49

Microfluidic cell ................................................................................................................................................... 49

Sensor.................................................................................................................................................................. 49

Tubing.................................................................................................................................................................. 49

Check valves ........................................................................................................................................................ 49

Dust..................................................................................................................................................................... 50

ORDER PARTS AND CONSUMABLES ...........................................................................................51

List of Tables

Table 1 - P4SPR technical specification........................................................................................ 5

Table 2 - Microfluidic cell technical specification ........................................................................ 6

Table 3 - Analytical performances technical specification ......................................................... 6

Table 4 List of parts and consumables ...................................................................................... 51

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Table of Figures 
Figure 1- Device Components ...................................................................................................... 3 
Figure 2 - P4SPR Installed Setup (version may slightly vary)...................................................... 9 
Figure 3 - SPR sensor chip handing............................................................................................ 10 
Figure 4 Aligning holes on the cell holder arm with microfluidic cells.................................... 11 
Figure 5 Inserting connectors through aligned holes............................................................... 11 
Figure 6 Connector in microfluidic cell ...................................................................................... 12 
Figure 7 P4SPR installer folder ................................................................................................... 13 
Figure 8 P4SPR installation directory ......................................................................................... 13 
Figure 9 P4SPR installation license agreement......................................................................... 14 
Figure 10 P4SPR installation Review .......................................................................................... 14 
Figure 11 P4SPR installation complete ...................................................................................... 15 
Figure 12 Restart PC .................................................................................................................... 15 
Figure 13 P4SPR Control Software Icon..................................................................................... 16 
Figure 14 P4SPR Optical Detector Driver in Device Manager .................................................. 16 
Figure 15 P4SPR Optical Detector Driver Property WIndow .................................................... 17 
Figure 16 Update P4SPR Optical Detector Driver ..................................................................... 17 
Figure 17 P4SPR Optical Detector Driver Path (Left) and USB4000 folder unzipped on USB 
stick............................................................................................................................................... 18 
Figure 18 Overall P4SPR Setup Picture ...................................................................................... 20 
Figure 19 SPR Sensor Setup Picture........................................................................................... 20 
Figure 20 Overview of P4SPR Control GraphicalUser Interface............................................... 22 
Figure 21 P4SPR Software On Button ........................................................................................ 23 
Figure 22 P4SPR Software Connect Device Window................................................................. 23 
Figure 23 P4SPR Software Connection Confirmatino Window ................................................ 24 
Figure 24 GUI Message board .................................................................................................... 24 
Figure 25 GUI Spectroscopy Tab Initial Display......................................................................... 25 
Figure 26 GUI Data Acquisition Tab ........................................................................................... 26 
Figure 27 GUI Data Acquiring Tab.............................................................................................. 27 
Figure 28 GUI Spectroscopy Tab ................................................................................................ 28 
Figure 29 GUI Parameter Tab ..................................................................................................... 29 
Figure 30 GUI Graphical Display................................................................................................. 30 
Figure 31 GUI Cursor Control ..................................................................................................... 31 
Figure 32 GUI Channel Display ................................................................................................... 32 
Figure 33 GUI Segment of Interest Display ............................................................................... 33 
Figure 34 GUI Segment Table ..................................................................................................... 34 
Figure 35 GUI Average Display option ....................................................................................... 35 
Figure 36 GUI Data Recording .................................................................................................... 36 

viii 
 
Figure 37 GUI Graphics Display .................................................................................................. 37 
Figure 38 GUI Example : Segment Table.................................................................................... 38 
Figure 39 Raw Data...................................................................................................................... 40 
Figure 40 Overlaid Sensorgram Segments................................................................................ 40 
Figure 41 Shifts ............................................................................................................................ 41 
Figure 42 Data Structure in Saved Folder.................................................................................. 41 
Figure 43 Setting up the microfluidic cell .................................................................................. 43 
Figure 44 Transmission Spectra ................................................................................................. 44 
Figure 45 Troubleshooting Full Sensorgram ............................................................................. 44 
Figure 46 Air Bubbles in Microfluidic Channels ........................................................................ 44 
Figure 47 Large bubble Effect on Sensorgram graph............................................................... 45 
Figure 48 Large bubble effect on Transmission Spectra graph............................................... 45 
Figure 49 Leak Warning............................................................................................................... 46 
Figure 50 Weak LED intensity in channel A (white line) due to a leak..................................... 46 
Figure 51 Water Leak................................................................................................................... 46 
Figure 52 Transmission Spectra after clearing leak.................................................................. 47 
Figure 53 Signal saturation ......................................................................................................... 48 
 
 
 
 

General Information

Purpose of this manual

This user manual describes all the steps needed to run the Portable, 4-Channel Surface

Plasmon Resonance (P4SPR), from device setup to software installation, taking

measurements and finally analyzing the obtained data. Please read this guide carefully

before using the P4SPR and keep it available to ensure maximum device performance.

This manual is frequently updated. Contact Affinité Instruments

info@affiniteinstruments.com or visit our website at www.affiniteinstruments.com to get the

latest version.

Device overview

The P4SPR delivers high-quality Surface Plasmon Resonance (SPR) data wherever you

need it. Its proprietary optical design has no moving parts, making it compact and

robust. Additionally, the P4SPR is built on the benchmark SPR technology using thin

metallic film sensors similar to the state-of-the-art devices. P4SPR is USB powered with

each sample measured in triplicate along with a reference signal for high precision

calibrated data. The output data in TXT can be rapidly processed with your preferred

data processing software, or third-party software TraceDrawer™ to extract qualitative

and quantitative information such as concentration, binding, selectivity, kinetic and

affinity. Typical SPR applications of P4SPR are those related to biomolecular interactions,

with other applications such as measuring and monitoring small molecules and

nanoparticles.

Before you start

P4SPR Starter-Kit

Checklist

1. P4SPR hard shell plastic case

2. P4SPR instrument (1)

3. USB Cables (2 x 1 USB 2.0 mini-B)

4. Gold (Au) sensors or surface modified Gold sensors (10)

5. PDMS microfluidic cells (3)

6. Tubing and connectors

7. TraceDrawer™Licence for biomolecular analysis (optional)

Checklist may not apply for non starter-kits

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